Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P12_F_B14
(725 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 92 6e-20
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 77 3e-15
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 72 1e-13
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 44 2e-05
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 35 0.014
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 31 0.22
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 31 0.22
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 29 0.68
SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 27 2.1
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 2.7
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 26 4.8
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 92.3 bits (219), Expect = 6e-20
Identities = 48/126 (38%), Positives = 74/126 (58%)
Frame = +3
Query: 111 DSRGXGKXHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQAISKARSG 290
D G G+ IGD LRA GQNPT +++ + L P E + E FL + +
Sbjct: 16 DRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTL-PAE-VDMEQFLQVLNRPNGFDMP 73
Query: 291 DTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYEN 470
+F++G + FDKD G I ELR++L++LGEKLS++E+++LL+G G +NY +
Sbjct: 74 GDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGVPVKDGMVNYHD 133
Query: 471 FVHLIM 488
FV +I+
Sbjct: 134 FVQMIL 139
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 77.0 bits (181), Expect = 3e-15
Identities = 42/117 (35%), Positives = 67/117 (57%), Gaps = 3/117 (2%)
Frame = +3
Query: 144 QIGDALRALGQNPTESDVKKCTLHLKPDER--ISFEVFLPIYQAISKARSGDTANDFIEG 317
++G +R+LGQ+PT ++++ + D I F FL + K + D + E
Sbjct: 33 ELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMAR--KMKDTDNEEEVREA 90
Query: 318 LRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL-QGQEDSQGNINYENFVHLI 485
+ FDKDGNG+I+ EL H+L++LGE+LS +EV ++ + D G INYE F +I
Sbjct: 91 FKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVI 147
Score = 33.9 bits (74), Expect = 0.024
Identities = 17/62 (27%), Positives = 36/62 (58%), Gaps = 1/62 (1%)
Frame = +3
Query: 303 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLL-QGQEDSQGNINYENFVH 479
+F E FD+D +G I+S EL ++ +LG+ + E++ ++ + D G I++ F+
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLT 72
Query: 480 LI 485
++
Sbjct: 73 MM 74
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 71.7 bits (168), Expect = 1e-13
Identities = 40/122 (32%), Positives = 70/122 (57%), Gaps = 1/122 (0%)
Frame = +3
Query: 126 GKXHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQAISKARSGDTAND 305
G + +G LR+LG N T++++ K + L + I + F+ +K R ++ +
Sbjct: 24 GLIPTSHVGSVLRSLGINVTDAELAKLSNEL--GDAIDEKKFMSFVS--NKLRETESEEE 79
Query: 306 FIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ-GQEDSQGNINYENFVHL 482
+I+ R FDKD +G+I +A+ + TLGEKLSD+EV+ ++Q + G+ +Y +FV
Sbjct: 80 YIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQR 139
Query: 483 IM 488
IM
Sbjct: 140 IM 141
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 44.4 bits (100), Expect = 2e-05
Identities = 34/125 (27%), Positives = 54/125 (43%)
Frame = +3
Query: 111 DSRGXGKXHVAQIGDALRALGQNPTESDVKKCTLHLKPDERISFEVFLPIYQAISKARSG 290
D G G + L +L Q+ +E + + P I+ FL ++ S
Sbjct: 58 DKDGDGNIGREDVKTMLTSLNQDASEDSINHMFESINPP--INLAAFLTAMGSMLCRIS- 114
Query: 291 DTANDFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYEN 470
ND +E FD +G I + +R LS++G+++ EVE +L+ S G YE
Sbjct: 115 -PRNDLLEAFSTFDDTQSGKIPISTMRDALSSMGDRMDPQEVESILRSY-TSHGVFYYEK 172
Query: 471 FVHLI 485
FV I
Sbjct: 173 FVDAI 177
Score = 29.9 bits (64), Expect = 0.39
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = +3
Query: 312 EGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQ 431
E DKDG+G I +++ +L++L + S+D + + +
Sbjct: 52 EAFALLDKDGDGNIGREDVKTMLTSLNQDASEDSINHMFE 91
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 34.7 bits (76), Expect = 0.014
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = +3
Query: 321 RHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQ 491
RHF+K + ++ E L++LG +E L +S+ + YE F ++M+
Sbjct: 494 RHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEGVTYERFTEIVME 550
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 30.7 bits (66), Expect = 0.22
Identities = 18/64 (28%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Frame = +3
Query: 303 DFIEGLRHFDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQG-QEDSQGNINYENFVH 479
D E + FD D + I ELR + LG EV ++L+ + +G + E+FV
Sbjct: 38 DINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVR 97
Query: 480 LIMQ 491
++ +
Sbjct: 98 VMTE 101
Score = 25.4 bits (53), Expect = 8.3
Identities = 14/59 (23%), Positives = 32/59 (54%), Gaps = 2/59 (3%)
Frame = +3
Query: 300 NDFIEGLRHFDKDGNGFISSAE-LRHLLSTLGEKLSDDEVEQLLQ-GQEDSQGNINYEN 470
++ ++ LR FDK G G++ + +R + + E+ +E+++ + +D G I+ N
Sbjct: 73 SEVLKILRDFDKTGKGYLQMEDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRN 131
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 30.7 bits (66), Expect = 0.22
Identities = 16/52 (30%), Positives = 27/52 (51%)
Frame = +3
Query: 327 FDKDGNGFISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHL 482
FD G+I +LR + LGE L+ +++ QL+ + G ++ E F L
Sbjct: 21 FDVTHKGYIDFEDLRRSCAQLGENLTKEQL-QLMLDLAGTNGKVSREEFAEL 71
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 29.1 bits (62), Expect = 0.68
Identities = 19/57 (33%), Positives = 29/57 (50%)
Frame = -1
Query: 419 LYLIITKLLSESREQVSQFRRRDEPIAIFVKMAQTLNKVISSVATACFRYGLVNWQK 249
L+L+ L++ +Q + R + IAIF K + +K I SVA R+ L QK
Sbjct: 1377 LHLLFASLVAHKFDQPQHAQTRTKIIAIFFKDLYSPHKEIYSVAIDALRHVLSQNQK 1433
>SPAC1565.07c |||TATA binding protein interacting protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1220
Score = 27.5 bits (58), Expect = 2.1
Identities = 20/66 (30%), Positives = 37/66 (56%), Gaps = 4/66 (6%)
Frame = +3
Query: 243 EVFLPIYQAISKARSGDTANDFI--EGLRHFDKDGNGFISS-AELRHLLSTLGEK-LSDD 410
++F+ + I+K +G + I + RH DK GN F ++ EL +L +G+K L++
Sbjct: 643 DIFMSVTD-ITKIENGTKIYEEILQDCCRHIDKSGNEFTTAYLELLEVLLKVGQKYLAES 701
Query: 411 EVEQLL 428
+E +L
Sbjct: 702 LLEHIL 707
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 27.1 bits (57), Expect = 2.7
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +3
Query: 351 ISSAELRHLLSTLGEKLSDDEVEQLLQGQEDSQGNINYENFVHLIMQG 494
I A++ LLSTL E D ++EQ+ Q + + Y +F+ ++QG
Sbjct: 42 IVKAQILFLLSTLREDQYDTKLEQIRQLINKNAPRV-YHHFLRRLIQG 88
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 26.2 bits (55), Expect = 4.8
Identities = 16/67 (23%), Positives = 32/67 (47%)
Frame = -1
Query: 587 LTVSHYGSNCMVTDLVSLQIKVLQQMMT*NSALHDEVNKVLIVDISLRVFLSLQELLYLI 408
L S+Y ++ D V Q+K L Q +S + + + + RV S+ + L+L+
Sbjct: 539 LETSYYNCMTVLEDEVIAQLKSLLQYSKTSSQMFTTLMRFQPLFFRTRVRTSISDCLHLL 598
Query: 407 ITKLLSE 387
+ ++ E
Sbjct: 599 VNRIKQE 605
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,561,637
Number of Sequences: 5004
Number of extensions: 49086
Number of successful extensions: 157
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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