Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P12_F_B12
(692 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_A7T6B8 Cluster: Predicted protein; n=1; Nematostella ve... 195 7e-49
UniRef50_Q4S0L0 Cluster: Malic enzyme; n=2; Tetraodon nigrovirid... 191 2e-47
UniRef50_Q5BX10 Cluster: Malic enzyme; n=1; Schistosoma japonicu... 184 2e-45
UniRef50_Q16798 Cluster: NADP-dependent malic enzyme, mitochondr... 183 4e-45
UniRef50_P23368 Cluster: NAD-dependent malic enzyme, mitochondri... 182 7e-45
UniRef50_P48163 Cluster: NADP-dependent malic enzyme; n=63; Euka... 173 3e-42
UniRef50_P06801 Cluster: NADP-dependent malic enzyme; n=52; cell... 169 4e-41
UniRef50_A0L5P5 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 165 1e-39
UniRef50_A2ZQ54 Cluster: Malic enzyme; n=9; Oryza sativa|Rep: Ma... 163 4e-39
UniRef50_Q89G76 Cluster: Malic enzyme; n=3; cellular organisms|R... 160 3e-38
UniRef50_P16243 Cluster: NADP-dependent malic enzyme, chloroplas... 159 6e-38
UniRef50_A7CWP9 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 157 2e-37
UniRef50_P78715 Cluster: Malic enzyme, hydrogenosomal precursor;... 154 2e-36
UniRef50_A7PC00 Cluster: Chromosome chr2 scaffold_11, whole geno... 148 1e-34
UniRef50_Q0AIF8 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 146 6e-34
UniRef50_A7IMB8 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 144 2e-33
UniRef50_Q016K2 Cluster: NADP dependent malic enzyme; n=2; Ostre... 142 7e-33
UniRef50_Q8D911 Cluster: NAD-dependent malic enzyme; n=187; cell... 141 1e-32
UniRef50_Q86NT5 Cluster: Malic enzyme; n=2; Drosophila melanogas... 138 2e-31
UniRef50_Q875H8 Cluster: Malic enzyme; n=1; Mucor circinelloides... 134 2e-30
UniRef50_Q8I8I4 Cluster: Malic enzyme; n=4; Eukaryota|Rep: Malic... 130 3e-29
UniRef50_P37221 Cluster: NAD-dependent malic enzyme 62 kDa isofo... 130 4e-29
UniRef50_A1ZAF7 Cluster: Malic enzyme; n=5; Sophophora|Rep: Mali... 129 6e-29
UniRef50_Q00XN9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Ma... 128 1e-28
UniRef50_A4RQC9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Ma... 126 7e-28
UniRef50_Q4QAQ6 Cluster: Malic enzyme, putative; n=20; Trypanoso... 124 2e-27
UniRef50_A2EKE3 Cluster: Malic enzyme; n=14; Trichomonadidae|Rep... 122 1e-26
UniRef50_A6SA55 Cluster: Malic enzyme; n=2; Sclerotiniaceae|Rep:... 121 1e-26
UniRef50_Q7SHJ8 Cluster: Malic enzyme; n=12; Pezizomycotina|Rep:... 120 3e-26
UniRef50_A1SVL3 Cluster: Malic enzyme aka malate dehydrogenase (... 120 3e-26
UniRef50_A0Q531 Cluster: NAD-dependent malic enzyme; n=10; Franc... 119 6e-26
UniRef50_Q9RYN4 Cluster: Malate oxidoreductase; n=6; Deinococci|... 119 8e-26
UniRef50_P45868 Cluster: Probable NAD-dependent malic enzyme 2; ... 118 1e-25
UniRef50_UPI0000D9F768 Cluster: PREDICTED: similar to Y48B6A.12,... 116 5e-25
UniRef50_Q4PC56 Cluster: Malic enzyme; n=1; Ustilago maydis|Rep:... 115 1e-24
UniRef50_Q4X1Z2 Cluster: NADP-dependent malic enzyme MaeA; n=11;... 113 3e-24
UniRef50_A3QW96 Cluster: Malic enzyme; n=10; Tigriopus californi... 112 9e-24
UniRef50_Q9HE50 Cluster: Malic enzyme; n=6; Pezizomycotina|Rep: ... 111 2e-23
UniRef50_A2QY66 Cluster: Malic enzyme; n=2; cellular organisms|R... 111 2e-23
UniRef50_Q01AM5 Cluster: NADP+-dependent malic enzyme; n=2; Ostr... 109 5e-23
UniRef50_Q7K3R0 Cluster: Malic enzyme; n=2; Sophophora|Rep: Mali... 107 3e-22
UniRef50_Q95061 Cluster: Malic enzyme; n=2; Giardia intestinalis... 103 5e-21
UniRef50_Q5CS07 Cluster: Malic enzyme; n=2; Cryptosporidium|Rep:... 103 5e-21
UniRef50_Q6AL43 Cluster: Related to NAD-dependent malic enzyme; ... 101 2e-20
UniRef50_Q8Y5Y8 Cluster: Lmo1915 protein; n=15; Firmicutes|Rep: ... 100 4e-20
UniRef50_P40375 Cluster: NAD-dependent malic enzyme; n=3; Schizo... 100 4e-20
UniRef50_A3BK03 Cluster: Malic enzyme; n=2; Oryza sativa|Rep: Ma... 95 2e-18
UniRef50_Q48796 Cluster: Malolactic enzyme; n=49; Bacteria|Rep: ... 95 2e-18
UniRef50_P36013 Cluster: NAD-dependent malic enzyme, mitochondri... 90 4e-17
UniRef50_Q9S4T5 Cluster: NAD-malate oxidoreductase homolog; n=15... 90 5e-17
UniRef50_Q2HCG7 Cluster: Malic enzyme; n=1; Chaetomium globosum|... 86 7e-16
UniRef50_A6XP71 Cluster: Malic enzyme protein 2; n=2; Mucoromyco... 85 2e-15
UniRef50_Q5KEY3 Cluster: Malic enzyme; n=1; Filobasidiella neofo... 84 4e-15
UniRef50_A4SKB8 Cluster: NAD-dependent malic enzyme; n=2; Aeromo... 81 3e-14
UniRef50_Q5K758 Cluster: Malic enzyme; n=1; Filobasidiella neofo... 79 1e-13
UniRef50_Q5KBK5 Cluster: Nad-dependent malic enzyme, putative; n... 77 3e-13
UniRef50_A3YYQ0 Cluster: Malate oxidoreductase; n=1; Synechococc... 73 9e-12
UniRef50_A4RZU1 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Ma... 61 3e-08
UniRef50_Q8S484 Cluster: Putative NADP-dependent malic enzyme; n... 45 0.002
UniRef50_UPI0000DB7FF6 Cluster: PREDICTED: hypothetical protein,... 39 0.10
UniRef50_Q116W6 Cluster: Putative uncharacterized protein; n=1; ... 34 2.9
UniRef50_Q08PM3 Cluster: Hemin ABC transporter, periplasmic hemi... 34 2.9
UniRef50_A1CKF3 Cluster: Stress response protein (Ish1), putativ... 34 2.9
UniRef50_Q4SHK3 Cluster: Chromosome 5 SCAF14581, whole genome sh... 34 3.8
UniRef50_Q4RNM6 Cluster: Chromosome 21 SCAF15012, whole genome s... 33 5.0
UniRef50_UPI0001560FE5 Cluster: PREDICTED: similar to KIAA2007 p... 33 6.6
UniRef50_Q1EI20 Cluster: Putative uncharacterized protein; n=2; ... 33 8.8
UniRef50_A0WC26 Cluster: Multi-sensor hybrid histidine kinase pr... 33 8.8
>UniRef50_A7T6B8 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 365
Score = 195 bits (476), Expect = 7e-49
Identities = 87/143 (60%), Positives = 112/143 (78%)
Frame = +2
Query: 65 LSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKY 244
+ G D ++ LNKG+AFT+EERQ+LGIHGLLPP V +QE Q + + R N L KY
Sbjct: 13 IRGTDIMRDSHLNKGLAFTLEERQILGIHGLLPPCVISQEIQAQRVYRELQRKPNDLEKY 72
Query: 245 IYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGH 424
I LM LL+RNE LF+R + D E+MPIVYTPTVGLAC+K+G+++RRPRGLFI+IHDKGH
Sbjct: 73 IQLMALLERNESLFFRVLFDYTEELMPIVYTPTVGLACRKYGMIFRRPRGLFISIHDKGH 132
Query: 425 VYDVLKNWPETDVRAIVVTDGER 493
+ D++ NWP T+V+AIV+TDGER
Sbjct: 133 IRDIVSNWPTTEVKAIVMTDGER 155
>UniRef50_Q4S0L0 Cluster: Malic enzyme; n=2; Tetraodon
nigroviridis|Rep: Malic enzyme - Tetraodon nigroviridis
(Green puffer)
Length = 694
Score = 191 bits (465), Expect = 2e-47
Identities = 87/152 (57%), Positives = 112/152 (73%)
Frame = +2
Query: 38 SGDGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSID 217
+ +G + G D ++P LNKGMAFT+EER +GIHGLLPP +Q+ QV S +
Sbjct: 51 ASEGSVRTKKRGYDITRNPHLNKGMAFTLEERLQMGIHGLLPPCFLSQDVQVLRVMKSYE 110
Query: 218 RYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGL 397
NPL+KYI LM L DRNE LFYR + ++ + MPIVYTPTVGLACQ++GL +RRPRGL
Sbjct: 111 TRSNPLDKYILLMTLQDRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYGLAFRRPRGL 170
Query: 398 FITIHDKGHVYDVLKNWPETDVRAIVVTDGER 493
FITIHD+GH+ +L +WPE D++A+VVTDGER
Sbjct: 171 FITIHDRGHIATMLNSWPEEDIKAVVVTDGER 202
>UniRef50_Q5BX10 Cluster: Malic enzyme; n=1; Schistosoma
japonicum|Rep: Malic enzyme - Schistosoma japonicum
(Blood fluke)
Length = 216
Score = 184 bits (447), Expect = 2e-45
Identities = 82/143 (57%), Positives = 107/143 (74%)
Frame = +2
Query: 65 LSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKY 244
L G+D ++ P N+G AFT+ ERQLLGIHGLLPP V T E+QV ++ + L +Y
Sbjct: 52 LLGIDVVRDPRTNRGTAFTVNERQLLGIHGLLPPSVLTLEQQVSKMLANLKNMNDNLQRY 111
Query: 245 IYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGH 424
IYL L DRNE LFY+ V ++V MP++YTPTVGLACQ++G+V+RRPRGL+ITIHD+ H
Sbjct: 112 IYLTSLQDRNEALFYKLVIEHVEYCMPLIYTPTVGLACQRYGVVFRRPRGLYITIHDRHH 171
Query: 425 VYDVLKNWPETDVRAIVVTDGER 493
+ ++L NWPE V+AIV TDGER
Sbjct: 172 IPEILNNWPEPIVKAIVFTDGER 194
>UniRef50_Q16798 Cluster: NADP-dependent malic enzyme, mitochondrial
precursor; n=15; Bilateria|Rep: NADP-dependent malic
enzyme, mitochondrial precursor - Homo sapiens (Human)
Length = 604
Score = 183 bits (445), Expect = 4e-45
Identities = 84/141 (59%), Positives = 107/141 (75%)
Frame = +2
Query: 71 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIY 250
G D ++P LNKGMAFT+EER LGIHGL+PP +Q+ Q+ +R ++ L+KYI
Sbjct: 50 GYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYII 109
Query: 251 LMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVY 430
LM L DRNE LFYR + +V + MPIVYTPTVGLACQ +GL +RRPRGLFITIHDKGH+
Sbjct: 110 LMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHLA 169
Query: 431 DVLKNWPETDVRAIVVTDGER 493
+L +WPE +++A+VVTDGER
Sbjct: 170 TMLNSWPEDNIKAVVVTDGER 190
>UniRef50_P23368 Cluster: NAD-dependent malic enzyme, mitochondrial
precursor; n=53; Eumetazoa|Rep: NAD-dependent malic
enzyme, mitochondrial precursor - Homo sapiens (Human)
Length = 584
Score = 182 bits (443), Expect = 7e-45
Identities = 76/135 (56%), Positives = 107/135 (79%)
Frame = +2
Query: 89 HPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGLLD 268
+P NKGMAFT++ERQ+LG+ GLLPP+++TQ+ Q ++ + +PL KYIY+MG+ +
Sbjct: 31 NPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRNLKKMTSPLEKYIYIMGIQE 90
Query: 269 RNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNW 448
RNE LFYR + D++ +MPIVYTPTVGLAC ++G ++RRP+GLFI+I D+GHV ++ NW
Sbjct: 91 RNEKLFYRILQDDIESLMPIVYTPTVGLACSQYGHIFRRPKGLFISISDRGHVRSIVDNW 150
Query: 449 PETDVRAIVVTDGER 493
PE V+A+VVTDGER
Sbjct: 151 PENHVKAVVVTDGER 165
>UniRef50_P48163 Cluster: NADP-dependent malic enzyme; n=63;
Eukaryota|Rep: NADP-dependent malic enzyme - Homo
sapiens (Human)
Length = 572
Score = 173 bits (421), Expect = 3e-42
Identities = 78/136 (57%), Positives = 101/136 (74%)
Frame = +2
Query: 86 KHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGLL 265
++P LNK +AFT+EERQ L IHGLLPP +QE QV + + + ++Y+ LM L
Sbjct: 20 RNPHLNKDLAFTLEERQQLNIHGLLPPSFNSQEIQVLRVVKNFEHLNSDFDRYLLLMDLQ 79
Query: 266 DRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKN 445
DRNE LFYR + ++ + MPIVYTPTVGLACQ++ LV+R+PRGLFITIHD+GH+ VL
Sbjct: 80 DRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYSLVFRKPRGLFITIHDRGHIASVLNA 139
Query: 446 WPETDVRAIVVTDGER 493
WPE ++AIVVTDGER
Sbjct: 140 WPEDVIKAIVVTDGER 155
>UniRef50_P06801 Cluster: NADP-dependent malic enzyme; n=52;
cellular organisms|Rep: NADP-dependent malic enzyme -
Mus musculus (Mouse)
Length = 572
Score = 169 bits (412), Expect = 4e-41
Identities = 79/136 (58%), Positives = 100/136 (73%)
Frame = +2
Query: 86 KHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGLL 265
+ P LNK +AFT+EERQ L IHGLLPP + +QE QV + +R + ++Y+ LM L
Sbjct: 20 RDPHLNKDLAFTLEERQQLNIHGLLPPCIISQELQVLRIIKNFERLNSDFDRYLLLMDLQ 79
Query: 266 DRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKN 445
DRNE LFY + +V + MPIVYTPTVGLACQ++ L +R+PRGLFI+IHDKGH+ VL
Sbjct: 80 DRNEKLFYSVLMSDVEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDKGHIASVLNA 139
Query: 446 WPETDVRAIVVTDGER 493
WPE V+AIVVTDGER
Sbjct: 140 WPEDVVKAIVVTDGER 155
>UniRef50_A0L5P5 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=1;
Magnetococcus sp. MC-1|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Magnetococcus
sp. (strain MC-1)
Length = 556
Score = 165 bits (400), Expect = 1e-39
Identities = 78/146 (53%), Positives = 101/146 (69%)
Frame = +2
Query: 56 TSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 235
T + L+ L P +NKG+AFT EER L + GLLPPRV+T E Q+ + N L
Sbjct: 14 TGHANPLEILNDPYMNKGVAFTEEERDLFHLRGLLPPRVQTMEAQLGRALDNFRCKPNDL 73
Query: 236 NKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHD 415
KYI+L GL +RNE LFYR V N+ EM+PI+YTPTVG ACQ +G ++RRP+G+FI+I+D
Sbjct: 74 EKYIFLTGLQERNETLFYRLVMTNIEEMLPIIYTPTVGKACQTYGHIFRRPQGMFISIND 133
Query: 416 KGHVYDVLKNWPETDVRAIVVTDGER 493
KG + ++L NW DVR IVVTDG R
Sbjct: 134 KGRIAELLGNWVHKDVRVIVVTDGSR 159
>UniRef50_A2ZQ54 Cluster: Malic enzyme; n=9; Oryza sativa|Rep: Malic
enzyme - Oryza sativa subsp. japonica (Rice)
Length = 613
Score = 163 bits (396), Expect = 4e-39
Identities = 72/142 (50%), Positives = 105/142 (73%)
Frame = +2
Query: 68 SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYI 247
SG L+ P NKG+AF+ +ER + GLLPP V +Q+ QV+ ++ +Y PL +Y+
Sbjct: 99 SGYTLLRDPHHNKGLAFSEKERDAHYLRGLLPPAVVSQDLQVKKIMHNLRQYSVPLQRYM 158
Query: 248 YLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHV 427
+M L +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G ++R+P+GL++++ DKG V
Sbjct: 159 AMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRQPQGLYVSLKDKGKV 218
Query: 428 YDVLKNWPETDVRAIVVTDGER 493
DVL+NWPE +++ IVVTDGER
Sbjct: 219 LDVLRNWPERNIQVIVVTDGER 240
>UniRef50_Q89G76 Cluster: Malic enzyme; n=3; cellular organisms|Rep:
Malic enzyme - Bradyrhizobium japonicum
Length = 531
Score = 160 bits (388), Expect = 3e-38
Identities = 76/137 (55%), Positives = 96/137 (70%)
Frame = +2
Query: 83 LKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGL 262
L+ P LNKG AFT ER LG+ GLLPP V T E QV+ ++ L KY+ L L
Sbjct: 4 LRDPLLNKGTAFTEAERAALGLRGLLPPCVLTMETQVDRVLTNLRMLPTDLEKYVALNAL 63
Query: 263 LDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLK 442
DRNE LF+R V DN+ E+ PI+YTPTVGLACQK+GL+++RPRG+FI+ D+G + ++LK
Sbjct: 64 HDRNEALFFRVVVDNIDEIQPIIYTPTVGLACQKYGLIFQRPRGMFISSRDRGQIAEILK 123
Query: 443 NWPETDVRAIVVTDGER 493
NWP R IVVTDGER
Sbjct: 124 NWP-YPARLIVVTDGER 139
>UniRef50_P16243 Cluster: NADP-dependent malic enzyme, chloroplast
precursor; n=79; Magnoliophyta|Rep: NADP-dependent malic
enzyme, chloroplast precursor - Zea mays (Maize)
Length = 636
Score = 159 bits (386), Expect = 6e-38
Identities = 72/146 (49%), Positives = 103/146 (70%)
Frame = +2
Query: 56 TSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPL 235
TS SG L+ P NKG+AFT EER + GLLPP V +QE Q++ ++ +Y+ PL
Sbjct: 92 TSVASGYTLLRDPHHNKGLAFTEEERDGHYLRGLLPPAVLSQELQIKKFMNTLRQYQTPL 151
Query: 236 NKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHD 415
+YI +M L + +E LFY+ + DNV E++P VYTPTVG ACQK+G ++ RP+GL++++ D
Sbjct: 152 QRYIAMMNLQETDERLFYKLLIDNVVELLPFVYTPTVGEACQKYGSIFGRPQGLYVSLKD 211
Query: 416 KGHVYDVLKNWPETDVRAIVVTDGER 493
KG V +VL+NWP +++ I VTDGER
Sbjct: 212 KGKVLEVLRNWPHRNIQVICVTDGER 237
>UniRef50_A7CWP9 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=1;
Opitutaceae bacterium TAV2|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Opitutaceae
bacterium TAV2
Length = 561
Score = 157 bits (381), Expect = 2e-37
Identities = 77/159 (48%), Positives = 104/159 (65%)
Frame = +2
Query: 17 DRIGLWGSGDGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVE 196
D++ G + L G L LNKG AF+ ER LG+ GLLPPRV T E+Q +
Sbjct: 6 DQVSSLGLSARGARASLRGTALLGDSVLNKGTAFSERERDALGLRGLLPPRVFTLEQQEQ 65
Query: 197 LCKLSIDRYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLV 376
++ + + + KYIYL L RNE LFYR + ++ EM+P+VYTPTVG AC ++G
Sbjct: 66 RALNAMAKKPSAIEKYIYLTTLQSRNETLFYRLLTNHAEEMIPLVYTPTVGQACLEYGAN 125
Query: 377 YRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVTDGER 493
+RRPRGLFI+I D+G + ++L++WP TDVR IVVTDGER
Sbjct: 126 FRRPRGLFISIKDRGRIAEILRHWPITDVRMIVVTDGER 164
>UniRef50_P78715 Cluster: Malic enzyme, hydrogenosomal precursor;
n=1; Neocallimastix frontalis|Rep: Malic enzyme,
hydrogenosomal precursor - Neocallimastix frontalis
(Rumen fungus)
Length = 592
Score = 154 bits (374), Expect = 2e-36
Identities = 69/142 (48%), Positives = 96/142 (67%)
Frame = +2
Query: 68 SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYI 247
+GLD L P LNKG AFT +E+ LGI GL+PPR ++ E Q + CK ++D+ +PL K+I
Sbjct: 46 TGLDILNDPKLNKGSAFTADEKDRLGIRGLVPPRPQSLEAQYKRCKTNLDKISDPLEKFI 105
Query: 248 YLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHV 427
YL L +RNE L+Y+ + +N E+ PI+YTP VG ACQKF ++ + RG++ + D+G +
Sbjct: 106 YLNHLQNRNETLYYKMILENFVELAPIIYTPVVGEACQKFHKIFTQTRGMYFSTADRGQM 165
Query: 428 YDVLKNWPETDVRAIVVTDGER 493
V NWP DV IVVTDG R
Sbjct: 166 SAVAANWPYDDVDVIVVTDGSR 187
>UniRef50_A7PC00 Cluster: Chromosome chr2 scaffold_11, whole genome
shotgun sequence; n=3; Vitis vinifera|Rep: Chromosome
chr2 scaffold_11, whole genome shotgun sequence - Vitis
vinifera (Grape)
Length = 573
Score = 148 bits (359), Expect = 1e-34
Identities = 68/142 (47%), Positives = 97/142 (68%)
Frame = +2
Query: 68 SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYI 247
SG L+ P NKG+AFT +ER + GLLPP V Q+ Q + ++ +Y+ PL +Y+
Sbjct: 196 SGYTLLRDPHHNKGLAFTEKERDAHYLRGLLPPAVLNQDLQEKRLMHNLRQYKVPLQRYM 255
Query: 248 YLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHV 427
+M +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G ++RRP+ L+I G +
Sbjct: 256 AMMDFQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQSLYIIDFFMGKI 315
Query: 428 YDVLKNWPETDVRAIVVTDGER 493
+VLKNWPE ++ IVVT+GER
Sbjct: 316 LEVLKNWPERSIQVIVVTNGER 337
>UniRef50_Q0AIF8 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=2;
Nitrosomonas|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Nitrosomonas
eutropha (strain C71)
Length = 536
Score = 146 bits (353), Expect = 6e-34
Identities = 70/143 (48%), Positives = 94/143 (65%)
Frame = +2
Query: 65 LSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKY 244
L G L P NK AFT EER+ G+ GLLP V ++Q + ++ + + KY
Sbjct: 4 LYGKTLLNDPVQNKSTAFTREEREHYGLQGLLPYGVTDIKKQQQRVLANLRNKSSNIEKY 63
Query: 245 IYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGH 424
IYL LL+RN+ LFYR + D++ E+MP+VYTPTVG AC K ++R+P+G +IT D+G
Sbjct: 64 IYLNDLLERNQQLFYRTLVDHIGEIMPLVYTPTVGEACVKLSHIFRKPQGFYITPEDRGE 123
Query: 425 VYDVLKNWPETDVRAIVVTDGER 493
+ LKNWPETDV+ IVVTDGER
Sbjct: 124 IISRLKNWPETDVQIIVVTDGER 146
>UniRef50_A7IMB8 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=2;
Alphaproteobacteria|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Xanthobacter
sp. (strain Py2)
Length = 550
Score = 144 bits (349), Expect = 2e-33
Identities = 69/147 (46%), Positives = 98/147 (66%)
Frame = +2
Query: 53 PTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENP 232
P S LSGL+ L P NKG A+T ++R+ LG+ GLLP V+T + QVE +D ++
Sbjct: 13 PKSNLSGLNLLHDPVRNKGTAYTRDDRRQLGLEGLLPHAVETLDRQVERVLDHLDHVKDE 72
Query: 233 LNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIH 412
L++Y YLM L RNE +FY+ V + +PI+Y PTV AC+ FG +YRRPRG++IT H
Sbjct: 73 LDQYSYLMDLEARNETVFYKAVMSDPKRFIPILYDPTVADACEAFGNLYRRPRGMYITRH 132
Query: 413 DKGHVYDVLKNWPETDVRAIVVTDGER 493
KG + +VL+NWP+ D+R + V+ G R
Sbjct: 133 MKGRMAEVLRNWPQKDIRFVCVSTGGR 159
>UniRef50_Q016K2 Cluster: NADP dependent malic enzyme; n=2;
Ostreococcus|Rep: NADP dependent malic enzyme -
Ostreococcus tauri
Length = 641
Score = 142 bits (344), Expect = 7e-33
Identities = 65/143 (45%), Positives = 98/143 (68%)
Frame = +2
Query: 65 LSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKY 244
+SG++ L+ NKGM+F +ER L + GLLPP V Q QVE + R + + K+
Sbjct: 81 ISGVELLRSGRYNKGMSFARDERDRLNLRGLLPPAVFDQATQVERVIERLRRVTSGVEKH 140
Query: 245 IYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGH 424
+L L +RNE LFYR V D++ E++P++ PTV C++ GL+YR+PRGL++++ DKG
Sbjct: 141 AWLPALYERNERLFYRVVKDHLEELLPVLAEPTVWQVCREAGLMYRQPRGLYVSMQDKGS 200
Query: 425 VYDVLKNWPETDVRAIVVTDGER 493
VY +LKNWP +V+A+V+TDG+R
Sbjct: 201 VYRLLKNWPVRNVKAVVLTDGQR 223
>UniRef50_Q8D911 Cluster: NAD-dependent malic enzyme; n=187;
cellular organisms|Rep: NAD-dependent malic enzyme -
Vibrio vulnificus
Length = 562
Score = 141 bits (342), Expect = 1e-32
Identities = 66/137 (48%), Positives = 91/137 (66%)
Frame = +2
Query: 83 LKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGL 262
L P LNKG AF+ EER + GLLP +T +EQVE + +E+ ++K+IYL +
Sbjct: 18 LSTPLLNKGSAFSAEERISFNLEGLLPETTETIQEQVERAYMQYKAFESDMDKHIYLRNI 77
Query: 263 LDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLK 442
D NE LFYR V +++ EMMPI+YTPTVG AC+ F +YRR RGLF++ ++ + D+L
Sbjct: 78 QDTNETLFYRLVQNHITEMMPIIYTPTVGAACENFSNIYRRGRGLFVSYANRDRIDDILN 137
Query: 443 NWPETDVRAIVVTDGER 493
N +V+ IVVTDGER
Sbjct: 138 NASNHNVKVIVVTDGER 154
>UniRef50_Q86NT5 Cluster: Malic enzyme; n=2; Drosophila
melanogaster|Rep: Malic enzyme - Drosophila melanogaster
(Fruit fly)
Length = 610
Score = 138 bits (333), Expect = 2e-31
Identities = 63/132 (47%), Positives = 91/132 (68%), Gaps = 1/132 (0%)
Frame = +2
Query: 101 NKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGLLDRNEH 280
NK +AFT+EERQ L IHGL+P V+T +EQ+ + + +E+ + +Y YL L E
Sbjct: 56 NKALAFTLEERQRLCIHGLMPACVRTYDEQMLAIESNFHSFESNVGRYRYLRALRQGYER 115
Query: 281 LFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWP-ET 457
L+++FV+ NV ++PI+YTPTVGLAC +G++YR G+ IT HD+GH+ +L NWP
Sbjct: 116 LYFQFVSKNVHAVLPIIYTPTVGLACTVYGMLYRGMTGIHITKHDRGHMKQILSNWPMRR 175
Query: 458 DVRAIVVTDGER 493
V+AI VTDG+R
Sbjct: 176 SVKAICVTDGQR 187
>UniRef50_Q875H8 Cluster: Malic enzyme; n=1; Mucor
circinelloides|Rep: Malic enzyme - Mucor circinelloides
Length = 617
Score = 134 bits (324), Expect = 2e-30
Identities = 62/141 (43%), Positives = 93/141 (65%)
Frame = +2
Query: 71 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIY 250
G++ L P L+KG AF+I ER+ L I GL+PPR + ++Q+ K ++D E PL K+++
Sbjct: 50 GVNLLHDPLLSKGTAFSIAERERLSIRGLVPPRCQEMDKQLLRIKRNLDACETPLAKFVF 109
Query: 251 LMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVY 430
L L DRNE L+Y+ + +++ E+ I+YTPTVGLA Q +YRR RG++ + D+G +
Sbjct: 110 LAALHDRNETLYYKIIMEHLEELAGIIYTPTVGLASQMSHSIYRRSRGMYFSSQDRGQMS 169
Query: 431 DVLKNWPETDVRAIVVTDGER 493
++ NWP V IVVTDG R
Sbjct: 170 AMVYNWPHDKVDVIVVTDGSR 190
>UniRef50_Q8I8I4 Cluster: Malic enzyme; n=4; Eukaryota|Rep: Malic
enzyme - Mastigamoeba balamuthi (Phreatamoeba balamuthi)
Length = 568
Score = 130 bits (314), Expect = 3e-29
Identities = 70/148 (47%), Positives = 87/148 (58%)
Frame = +2
Query: 50 QPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYEN 229
+P GL+G L G AFT EER+ L I GLLP V+T E Q +
Sbjct: 30 RPADGLAGPSWLTLAPT--GTAFTTEERKALRIRGLLPHAVETIEAQAARAYAQLTSQPT 87
Query: 230 PLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITI 409
PL KY+YL L RN+ LF+ V +V E +P+VYTPTVG C KF +R P GL+IT
Sbjct: 88 PLLKYLYLSQLSQRNQTLFFYLVQHHVEECVPLVYTPTVGEGCTKFSAEFRNPTGLYITP 147
Query: 410 HDKGHVYDVLKNWPETDVRAIVVTDGER 493
DKGHV ++L+NWP +V IVVTDG R
Sbjct: 148 EDKGHVAEILENWPH-EVEIIVVTDGGR 174
>UniRef50_P37221 Cluster: NAD-dependent malic enzyme 62 kDa isoform,
mitochondrial precursor; n=41; Eukaryota|Rep:
NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
precursor - Solanum tuberosum (Potato)
Length = 626
Score = 130 bits (313), Expect = 4e-29
Identities = 66/150 (44%), Positives = 91/150 (60%), Gaps = 10/150 (6%)
Frame = +2
Query: 74 LDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQV-----ELCKLSIDRYENPLN 238
LD L P NKG AF+ ER L I GLLPP V + E+Q+ +L +L + + P +
Sbjct: 50 LDILHDPWFNKGTAFSFTERDRLHIRGLLPPNVMSFEQQIARFMADLKRLEVQARDGPSD 109
Query: 239 KYIY-----LMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFI 403
Y+ L L DRNE L+Y+ + +N+ E PIVYTPTVGL CQK+ ++RRPRG++
Sbjct: 110 PYVLAKWRILNRLHDRNETLYYKVLMENIEEYAPIVYTPTVGLVCQKYSGLFRRPRGMYF 169
Query: 404 TIHDKGHVYDVLKNWPETDVRAIVVTDGER 493
+ D+G + ++ NWP V IVVTDG R
Sbjct: 170 SAEDRGEMMSMVYNWPADQVDMIVVTDGSR 199
>UniRef50_A1ZAF7 Cluster: Malic enzyme; n=5; Sophophora|Rep: Malic
enzyme - Drosophila melanogaster (Fruit fly)
Length = 603
Score = 129 bits (312), Expect = 6e-29
Identities = 57/131 (43%), Positives = 88/131 (67%)
Frame = +2
Query: 101 NKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGLLDRNEH 280
NKG+AFTI+ERQ LGI GL+P V++ ++Q+ + + + ++ YL + R+
Sbjct: 48 NKGLAFTIKERQRLGIMGLMPCSVRSMDDQMNAALANFEARPTDIARFTYLSAVHHRHRR 107
Query: 281 LFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETD 460
L+YRF+ +N+ + +PIVYTPTVG +GL +++ LFI+IHDKGH+ D++ NW +
Sbjct: 108 LYYRFIKENIEKSLPIVYTPTVGDVVATYGLNFQQAISLFISIHDKGHIRDLMHNWVDEG 167
Query: 461 VRAIVVTDGER 493
V+AI VTDG R
Sbjct: 168 VKAICVTDGGR 178
>UniRef50_Q00XN9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Malic
enzyme - Ostreococcus tauri
Length = 639
Score = 128 bits (309), Expect = 1e-28
Identities = 62/141 (43%), Positives = 87/141 (61%)
Frame = +2
Query: 71 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIY 250
G++ L +P NKG +FT ER+ LG+ GL+PPR +Q E PL+K+ +
Sbjct: 81 GIEVLHNPVYNKGTSFTASERERLGVRGLVPPRFFPIGQQATKIWAQNQSLERPLDKWQH 140
Query: 251 LMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVY 430
L L DRNE LFYR V D++ E+ PI+YTPTVG AC F + RR RG++ ++ D+G +
Sbjct: 141 LQDLKDRNETLFYRLVHDHIEELAPIIYTPTVGDACLNFSKLLRRARGMYFSVDDRGDIN 200
Query: 431 DVLKNWPETDVRAIVVTDGER 493
++ NW + V IVVTDG R
Sbjct: 201 SMMFNW-KRSVSVIVVTDGSR 220
>UniRef50_A4RQC9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Malic
enzyme - Ostreococcus lucimarinus CCE9901
Length = 549
Score = 126 bits (303), Expect = 7e-28
Identities = 60/137 (43%), Positives = 85/137 (62%)
Frame = +2
Query: 83 LKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGL 262
L P LNKG FT +R+ LG+ GLLP + +T E QV+ + +E +N+YI+L L
Sbjct: 13 LDSPSLNKGTGFTFTQRERLGLRGLLPRKYETVEIQVKRAWTQLCAFEEDMNRYIFLENL 72
Query: 263 LDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLK 442
+NE LFYR + +++ ++MPIVYTPTVG AC F +YR G++ + D G + +L
Sbjct: 73 HMQNERLFYRVLVEHLEDLMPIVYTPTVGEACINFDALYRNRCGMYFSRLDSGVMRRMLD 132
Query: 443 NWPETDVRAIVVTDGER 493
NWP + IVVTDG R
Sbjct: 133 NWPSPETEIIVVTDGGR 149
>UniRef50_Q4QAQ6 Cluster: Malic enzyme, putative; n=20;
Trypanosomatidae|Rep: Malic enzyme, putative -
Leishmania major
Length = 573
Score = 124 bits (300), Expect = 2e-27
Identities = 56/141 (39%), Positives = 92/141 (65%)
Frame = +2
Query: 71 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIY 250
G+D+L++ NKG AFT ER + + GLLPP V+T ++QVE ++R+ P+N+Y
Sbjct: 19 GVDYLRNRFTNKGTAFTAAERSHMNVEGLLPPSVETLDDQVERYWDQLNRFNEPINRYQL 78
Query: 251 LMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVY 430
L + + N L+Y + + + +PIVYTPTVG ACQ++G +Y++ GL++ + KG V
Sbjct: 79 LRNVQNTNVTLYYAILTRYLKQTLPIVYTPTVGEACQRYGDLYQKDHGLYLDVASKGKVR 138
Query: 431 DVLKNWPETDVRAIVVTDGER 493
+++N +T++ IV+TDG R
Sbjct: 139 RLIQNLRKTNIDVIVITDGSR 159
>UniRef50_A2EKE3 Cluster: Malic enzyme; n=14; Trichomonadidae|Rep:
Malic enzyme - Trichomonas vaginalis G3
Length = 567
Score = 122 bits (293), Expect = 1e-26
Identities = 62/143 (43%), Positives = 87/143 (60%), Gaps = 1/143 (0%)
Frame = +2
Query: 68 SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYI 247
+G+ L+ LNKG AFT EER + GLLP +V T++EQ + + PL KYI
Sbjct: 23 TGMTLLQDGDLNKGTAFTKEERDRFNLRGLLPYKVFTKDEQAARIRRQFELMPTPLLKYI 82
Query: 248 YLMGLLDRNEHLFYRFVADNVAE-MMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGH 424
+L ++N F+RF+ + E MPI+YTPTVG ACQK+ + RG++IT D G
Sbjct: 83 FLANEREKNSQSFWRFLFTHPPEETMPILYTPTVGEACQKWATHRQSYRGIYITPEDSGK 142
Query: 425 VYDVLKNWPETDVRAIVVTDGER 493
+ D+L+N+P D+R IVVTD R
Sbjct: 143 IKDILRNYPRQDIRCIVVTDAGR 165
>UniRef50_A6SA55 Cluster: Malic enzyme; n=2; Sclerotiniaceae|Rep:
Malic enzyme - Botryotinia fuckeliana B05.10
Length = 685
Score = 121 bits (292), Expect = 1e-26
Identities = 54/135 (40%), Positives = 88/135 (65%)
Frame = +2
Query: 89 HPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGLLD 268
HP L + +IE R+ GL+PP V++ + Q C ++ + P+ KY+YL L +
Sbjct: 97 HPIL-PDTSHSIELRRKNKTLGLVPPNVESHKLQTTRCLQQLNAKKTPIEKYMYLSNLRN 155
Query: 269 RNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNW 448
N HLFYR V +++ ++ P++YTPTVG AC ++ +Y++P GL+++ HD+G++ +VL NW
Sbjct: 156 NNVHLFYRLVQEHLTDITPLIYTPTVGEACLRWSEIYQQPEGLYLSYHDRGNLEEVLGNW 215
Query: 449 PETDVRAIVVTDGER 493
++DV VVTDG R
Sbjct: 216 RQSDVEMTVVTDGSR 230
>UniRef50_Q7SHJ8 Cluster: Malic enzyme; n=12; Pezizomycotina|Rep:
Malic enzyme - Neurospora crassa
Length = 611
Score = 120 bits (290), Expect = 3e-26
Identities = 65/153 (42%), Positives = 89/153 (58%), Gaps = 2/153 (1%)
Frame = +2
Query: 38 SGDGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSID 217
S G L G L HP NKG AFT EER+ +HGLLPP++++ E+QV+
Sbjct: 24 STSGPLECALKGTVLLNHPYFNKGSAFTKEERRDFALHGLLPPQIQSLEQQVQRAYEQYC 83
Query: 218 RYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGL 397
N L K ++ + ++NE LFYR + D++ EM +VYTPT G A Q + ++RRP G+
Sbjct: 84 SQPNDLAKNTFMTSMKEQNEVLFYRLLHDHLDEMFSVVYTPTEGEAIQNYSRLFRRPEGV 143
Query: 398 FITIHDKGHVYDVLKNW--PETDVRAIVVTDGE 490
F+ I+D V L W PE D+ IVVTDGE
Sbjct: 144 FLNINDMDSVKRDLAQWGKPE-DIDYIVVTDGE 175
>UniRef50_A1SVL3 Cluster: Malic enzyme aka malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=4;
Gammaproteobacteria|Rep: Malic enzyme aka malate
dehydrogenase (Oxaloacetate-decarboxylating) (NADP(+)) -
Psychromonas ingrahamii (strain 37)
Length = 571
Score = 120 bits (289), Expect = 3e-26
Identities = 63/156 (40%), Positives = 93/156 (59%), Gaps = 3/156 (1%)
Frame = +2
Query: 32 WGSGDG---QPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELC 202
W +G +P S L+G + L + LNK AFT +ER+ ++GLLPPRV+T E+Q++
Sbjct: 9 WEDSEGNVFRPVS-LTGNELLNNRTLNKSTAFTYQEREDFDLNGLLPPRVQTFEDQLKRV 67
Query: 203 KLSIDRYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYR 382
+ KY YL L DRNE LFY ++ N+ EM PI+YTPTVG ACQ+F ++
Sbjct: 68 YQGFSASSTDIEKYQYLRALQDRNETLFYALISRNIEEMTPIIYTPTVGKACQEFSHRFQ 127
Query: 383 RPRGLFITIHDKGHVYDVLKNWPETDVRAIVVTDGE 490
RGL++T + V + + + D++ IVVTD +
Sbjct: 128 IARGLYLTTDNIHDVGSMAREFTGKDIQIIVVTDSQ 163
>UniRef50_A0Q531 Cluster: NAD-dependent malic enzyme; n=10;
Francisella tularensis|Rep: NAD-dependent malic enzyme -
Francisella tularensis subsp. novicida (strain U112)
Length = 604
Score = 119 bits (287), Expect = 6e-26
Identities = 60/144 (41%), Positives = 88/144 (61%)
Frame = +2
Query: 59 SGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLN 238
+ L+G L + LNK +AF+ EER + G LP +V++ EEQ + +D N L
Sbjct: 23 TNLTGRQLLNNRVLNKDVAFSQEERIAFDLIGYLPEKVESLEEQAIRVRRQLDLKPNSLE 82
Query: 239 KYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 418
KY++L L D N LFY FV +N+ E+MPI+YTPTVG A QK+ +R+ GLFI+I K
Sbjct: 83 KYVFLNRLHDLNTTLFYHFVRENLEEIMPIIYTPTVGEAVQKYSSSFRKQSGLFISISHK 142
Query: 419 GHVYDVLKNWPETDVRAIVVTDGE 490
H+ +L+ + + ++VTDGE
Sbjct: 143 KHIARILERYEYNSIDLVLVTDGE 166
>UniRef50_Q9RYN4 Cluster: Malate oxidoreductase; n=6;
Deinococci|Rep: Malate oxidoreductase - Deinococcus
radiodurans
Length = 580
Score = 119 bits (286), Expect = 8e-26
Identities = 63/138 (45%), Positives = 81/138 (58%)
Frame = +2
Query: 71 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIY 250
G + + P LNKG AFT EER+ G+ GLL P+V + E +E + L K++Y
Sbjct: 37 GFNLTRIPLLNKGTAFTAEEREAHGLDGLLAPQVDSLEVLIERAYREFSKRGAALEKHVY 96
Query: 251 LMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVY 430
L L DRNE LFY ++ +V EM+PIVYTPTVG A +KF +YR PRGL ++
Sbjct: 97 LRNLQDRNEVLFYALLSHHVEEMLPIVYTPTVGDAVKKFSQIYRYPRGLTLSTRTIERAE 156
Query: 431 DVLKNWPETDVRAIVVTD 484
L N P DVR IV TD
Sbjct: 157 QALANVPLNDVRIIVATD 174
>UniRef50_P45868 Cluster: Probable NAD-dependent malic enzyme 2;
n=37; Bacteria|Rep: Probable NAD-dependent malic enzyme
2 - Bacillus subtilis
Length = 582
Score = 118 bits (285), Expect = 1e-25
Identities = 60/151 (39%), Positives = 93/151 (61%), Gaps = 2/151 (1%)
Frame = +2
Query: 44 DGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRY 223
+G + L G + L P LNKG+AF++EERQ LG+ GLLPP V + ++Q +
Sbjct: 26 EGHLETTLRGKEVLSIPTLNKGVAFSLEERQELGLEGLLPPTVLSLDQQAQRAYEQFQAQ 85
Query: 224 ENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFI 403
+ L + +YL L +RNE LFY+ + +++ EM+P+VYTPTVG A Q++ YRRP+G+++
Sbjct: 86 PDRLRQNVYLSDLANRNEVLFYKLLKNHLREMLPVVYTPTVGEAIQEYSHEYRRPQGIYL 145
Query: 404 TIHDKGHVYDVLKNWPET--DVRAIVVTDGE 490
+I + + +N T D+ IV TD E
Sbjct: 146 SIDNIDGIEKAFENLHATAGDIDLIVATDSE 176
>UniRef50_UPI0000D9F768 Cluster: PREDICTED: similar to Y48B6A.12,
partial; n=1; Macaca mulatta|Rep: PREDICTED: similar to
Y48B6A.12, partial - Macaca mulatta
Length = 456
Score = 116 bits (279), Expect = 5e-25
Identities = 55/114 (48%), Positives = 73/114 (64%)
Frame = +2
Query: 152 GLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIV 331
GLLPP+V + Q C L + N + KYIYL L DRNE L+++ + D+VAEMMPIV
Sbjct: 34 GLLPPKVLDLDVQSRRCYLQFSQNSNDIEKYIYLESLHDRNETLYFKLLVDHVAEMMPIV 93
Query: 332 YTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVTDGER 493
YTP VG ACQ FG ++R RGL+ + +KG+ +++ N D IVVTDG R
Sbjct: 94 YTPVVGKACQLFGHIFRNARGLYFNLSEKGNFKEMVWNSNVRDADIIVVTDGSR 147
>UniRef50_Q4PC56 Cluster: Malic enzyme; n=1; Ustilago maydis|Rep:
Malic enzyme - Ustilago maydis (Smut fungus)
Length = 634
Score = 115 bits (276), Expect = 1e-24
Identities = 57/143 (39%), Positives = 81/143 (56%), Gaps = 4/143 (2%)
Frame = +2
Query: 77 DH--LKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIY 250
DH + P N+ F+ E+R LG+ GLLPP ++ QV + PL K++
Sbjct: 77 DHRIISDPFPNQDTGFSYEKRDQLGLRGLLPPAKQSLNTQVLRVLHQLRSKSTPLEKHVM 136
Query: 251 LMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVY 430
L L N L+Y + N E++P++YTPTVG ACQKF +YRRP GL I++ DKG +
Sbjct: 137 LASLRQTNTRLYYATILANKEEILPLIYTPTVGEACQKFSHIYRRPEGLSISLEDKGKIA 196
Query: 431 DVLKNW--PETDVRAIVVTDGER 493
+++NW P R V+TDG R
Sbjct: 197 SIVENWPVPAGSPRIAVITDGSR 219
>UniRef50_Q4X1Z2 Cluster: NADP-dependent malic enzyme MaeA; n=11;
Pezizomycotina|Rep: NADP-dependent malic enzyme MaeA -
Aspergillus fumigatus (Sartorya fumigata)
Length = 661
Score = 113 bits (273), Expect = 3e-24
Identities = 47/121 (38%), Positives = 77/121 (63%)
Frame = +2
Query: 131 RQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGLLDRNEHLFYRFVADNV 310
R+ L +GL PPR ++ E Q C + + ++K++YL L N HLFYR V D++
Sbjct: 102 RKYLRTYGLTPPRAESYEIQKTRCLAQLALKQTAIDKFLYLSTLRKNNVHLFYRLVTDHL 161
Query: 311 AEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVTDGE 490
E+ P++YTP VG ACQK+ +Y++P G++++ D+G++ V+ NWP+ +V +TDG
Sbjct: 162 KELTPLIYTPVVGEACQKWSEIYQQPEGMYLSWEDRGNLAAVIANWPQPNVEITCITDGS 221
Query: 491 R 493
R
Sbjct: 222 R 222
>UniRef50_A3QW96 Cluster: Malic enzyme; n=10; Tigriopus
californicus|Rep: Malic enzyme - Tigriopus californicus
(Marine copepod)
Length = 322
Score = 112 bits (269), Expect = 9e-24
Identities = 60/160 (37%), Positives = 88/160 (55%), Gaps = 19/160 (11%)
Frame = +2
Query: 71 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDR------YENP 232
G + + P NKG+AF + ER L + GL+PPR+ + +EQ + R + P
Sbjct: 45 GRNLVSDPISNKGLAFPLSERDRLSVRGLVPPRILSIQEQERVIMDEYTRGWAARAEQEP 104
Query: 233 -------------LNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGL 373
+ K+ L + DRNE LFYR + DN +M PI+YTPTVG AC F
Sbjct: 105 EDEIIKSGVGPDNIRKWKVLQSVQDRNETLFYRILMDNFQDMAPIIYTPTVGWACSHFSQ 164
Query: 374 VYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVTDGER 493
+YRRPRG++ + D+G + ++ NW +V A+V+TDG R
Sbjct: 165 LYRRPRGMYFSHGDRGEMASMVYNWESDEVDAVVITDGSR 204
>UniRef50_Q9HE50 Cluster: Malic enzyme; n=6; Pezizomycotina|Rep:
Malic enzyme - Neurospora crassa
Length = 1023
Score = 111 bits (267), Expect = 2e-23
Identities = 51/130 (39%), Positives = 77/130 (59%)
Frame = +2
Query: 104 KGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGLLDRNEHL 283
KG +FT EER + GL+P ++ E+QVE I + +++Y+YL + +N L
Sbjct: 483 KGTSFTPEERVAKNLTGLIPHVMEDSEKQVERALKMIRTRQTDIDRYLYLSTIKSQNVDL 542
Query: 284 FYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDV 463
FYR + D+ EMMP+VYTPT+G C ++ +Y RP L+I+I + + +L+NWP
Sbjct: 543 FYRLLMDHAKEMMPLVYTPTIGDVCLQYSTLYTRPEALYISIKQRKSIRTILRNWPYPQP 602
Query: 464 RAIVVTDGER 493
VVTDG R
Sbjct: 603 EICVVTDGSR 612
>UniRef50_A2QY66 Cluster: Malic enzyme; n=2; cellular organisms|Rep:
Malic enzyme - Aspergillus niger
Length = 609
Score = 111 bits (266), Expect = 2e-23
Identities = 55/141 (39%), Positives = 84/141 (59%), Gaps = 1/141 (0%)
Frame = +2
Query: 71 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIY 250
G D L+ NKG AFT EER+ +HGLLPP ++T EEQV+ +N L K +
Sbjct: 57 GRDALQSCQFNKGSAFTEEERKTFKLHGLLPPNIQTLEEQVQRAYEQYSSRDNDLAKNTF 116
Query: 251 LMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVY 430
+ + +NE L+Y+ + ++ EM+ I+YTPT G A Q + ++R+P G F+ I D+ +
Sbjct: 117 MASMKAQNEVLYYKLIDTHLKEMLSIIYTPTEGDAIQNYSRLFRKPEGCFLNIRDQDRIE 176
Query: 431 DVLKNWPE-TDVRAIVVTDGE 490
+ L N+ +V IVV+DGE
Sbjct: 177 ECLSNFSRGEEVDYIVVSDGE 197
>UniRef50_Q01AM5 Cluster: NADP+-dependent malic enzyme; n=2;
Ostreococcus|Rep: NADP+-dependent malic enzyme -
Ostreococcus tauri
Length = 580
Score = 109 bits (263), Expect = 5e-23
Identities = 52/141 (36%), Positives = 83/141 (58%)
Frame = +2
Query: 71 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIY 250
G L+ +G+A +R+ G+ GL+P +E ++ ++ + E P KY
Sbjct: 48 GYASLRDASAYRGLA--TNDRRGKGVDGLIPAGEVGEEVEIARANAALAQCETPFEKYKQ 105
Query: 251 LMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVY 430
L+ L +E FYR + ++PI+YTPTVG AC KFG + +RP GL+++ +D G+V
Sbjct: 106 LVALQMTDESTFYRMLRSQTETLLPILYTPTVGEACVKFGTLVQRPMGLWVSSNDAGNVK 165
Query: 431 DVLKNWPETDVRAIVVTDGER 493
+++NWP TDV+ V+TDGER
Sbjct: 166 QLIRNWPATDVKIAVITDGER 186
>UniRef50_Q7K3R0 Cluster: Malic enzyme; n=2; Sophophora|Rep: Malic
enzyme - Drosophila melanogaster (Fruit fly)
Length = 633
Score = 107 bits (256), Expect = 3e-22
Identities = 54/143 (37%), Positives = 80/143 (55%)
Frame = +2
Query: 59 SGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLN 238
S + GL L NKG+AFT+ ER++L IHGLLP V+T +EQ E+C ++ + N +
Sbjct: 68 SKVDGLWMLNQSNYNKGLAFTLNERRVLSIHGLLPVAVRTIDEQAEICSNLLESFTNNVQ 127
Query: 239 KYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 418
+YIYL L RN LFY + N +P+ +++ +GL+I I D
Sbjct: 128 QYIYLTYLSRRNRRLFYYLLLSNPDRFVPMTDASGSIDLLMVHRMIHSMGQGLYICIKDL 187
Query: 419 GHVYDVLKNWPETDVRAIVVTDG 487
GHV +L NWP VR ++V++G
Sbjct: 188 GHVSQILSNWPFRCVRCLLVSNG 210
>UniRef50_Q95061 Cluster: Malic enzyme; n=2; Giardia
intestinalis|Rep: Malic enzyme - Giardia lamblia
(Giardia intestinalis)
Length = 557
Score = 103 bits (246), Expect = 5e-21
Identities = 55/138 (39%), Positives = 76/138 (55%), Gaps = 1/138 (0%)
Frame = +2
Query: 83 LKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGL 262
L++ NK AFT ER+ I LP RV+T E+Q+ C+ D P K++YL L
Sbjct: 9 LRNKDCNKDTAFTAAEREAHHIVARLPARVETIEQQISRCRAQFDVLTTPTEKWLYLTRL 68
Query: 263 LDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYR-RPRGLFITIHDKGHVYDVL 439
+ NE LF F + E++PIVYTPTVG AC + L+++ PRG ++ G V +
Sbjct: 69 QEVNETLFSGFCLKYLKEVLPIVYTPTVGTACSNYSLLWQGYPRGFYLNRTHLGKVKQIF 128
Query: 440 KNWPETDVRAIVVTDGER 493
WP + R IV TDG R
Sbjct: 129 DQWPYSP-RIIVATDGTR 145
>UniRef50_Q5CS07 Cluster: Malic enzyme; n=2; Cryptosporidium|Rep:
Malic enzyme - Cryptosporidium parvum Iowa II
Length = 614
Score = 103 bits (246), Expect = 5e-21
Identities = 52/147 (35%), Positives = 88/147 (59%), Gaps = 4/147 (2%)
Frame = +2
Query: 65 LSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKY 244
L G++ L++P NKG++FT+EER+ G+ GLLP + +T +EQV +I++ ++ + KY
Sbjct: 64 LKGIELLRNPFYNKGLSFTMEERKEYGLEGLLPAKYETIDEQVSRLWTAINKIDSNIGKY 123
Query: 245 IYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGL--VYRR--PRGLFITIH 412
+L + + LF+ + ++ P+VYTPTVG C +F R GL++
Sbjct: 124 TFLENIRSSSFILFHSLLDKYFKDLTPLVYTPTVGEGCIEFSRNPTIRNWLGSGLYLNKS 183
Query: 413 DKGHVYDVLKNWPETDVRAIVVTDGER 493
KG +Y++LK++ D+ IV+TDG R
Sbjct: 184 HKGRIYEILKDFKSDDIEIIVLTDGGR 210
>UniRef50_Q6AL43 Cluster: Related to NAD-dependent malic enzyme;
n=1; Desulfotalea psychrophila|Rep: Related to
NAD-dependent malic enzyme - Desulfotalea psychrophila
Length = 578
Score = 101 bits (241), Expect = 2e-20
Identities = 48/130 (36%), Positives = 73/130 (56%)
Frame = +2
Query: 101 NKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGLLDRNEH 280
NK AF+ +ER G+ G LPP + E QVE C + + E+ KYI++ L DRN
Sbjct: 35 NKSTAFSSKERDDFGLQGSLPPGFRDLEAQVENCHIKLGEKESEEEKYIFIRSLFDRNVT 94
Query: 281 LFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETD 460
L + + ++ + M I+YTPTVGLA QK+ ++R+ GL + D+L+ + D
Sbjct: 95 LAHALIQSDLEKFMGIIYTPTVGLAVQKYSAMFRQANGLHFSPDTIDQAEDILRRFAHRD 154
Query: 461 VRAIVVTDGE 490
+R VVTD +
Sbjct: 155 IRVAVVTDNQ 164
>UniRef50_Q8Y5Y8 Cluster: Lmo1915 protein; n=15; Firmicutes|Rep:
Lmo1915 protein - Listeria monocytogenes
Length = 547
Score = 100 bits (239), Expect = 4e-20
Identities = 54/144 (37%), Positives = 82/144 (56%), Gaps = 3/144 (2%)
Frame = +2
Query: 68 SGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYI 247
SG D++ +P LNKG AF+ EER + GLLPP ++T E+Q + I+ E PL+K+
Sbjct: 5 SGFDYMNNPLLNKGTAFSKEERASYQLDGLLPPIIETIEQQAVRIETQIENLETPLHKHR 64
Query: 248 YLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRP-RGLFITIHDKGH 424
L L + N L+Y V NV + +PI+YTPT+G A ++ Y P LF+
Sbjct: 65 LLTNLYNENRTLYYYVVTKNVTDYLPIIYTPTIGDAVIQYHKDYTAPDEALFVDAFAPEK 124
Query: 425 VYDVLKNWPET--DVRAIVVTDGE 490
+ +KN+ + ++ IV+TDGE
Sbjct: 125 LSASIKNYAKNNPNIDMIVITDGE 148
>UniRef50_P40375 Cluster: NAD-dependent malic enzyme; n=3;
Schizosaccharomyces pombe|Rep: NAD-dependent malic
enzyme - Schizosaccharomyces pombe (Fission yeast)
Length = 565
Score = 100 bits (239), Expect = 4e-20
Identities = 58/149 (38%), Positives = 86/149 (57%), Gaps = 7/149 (4%)
Frame = +2
Query: 65 LSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRY----ENP 232
L G+ L P NK AFT EERQ I LPP V+T ++QV+ C D+Y + P
Sbjct: 13 LKGVTLLNSPRYNKDTAFTPEERQKFEISSRLPPIVETLQQQVDRC---YDQYKAIGDEP 69
Query: 233 LNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITI- 409
L K +YL L N+ LFY ++ ++ EM+PI+YTPT G A ++F +YR P G ++ I
Sbjct: 70 LQKNLYLSQLSVTNQTLFYALISQHLIEMIPIIYTPTEGDAIKQFSDIYRYPEGCYLDID 129
Query: 410 -HDKGHVYDVLKNWPETD-VRAIVVTDGE 490
+D ++ L + ++D V I++TD E
Sbjct: 130 HNDLSYIKQQLSEFGKSDSVEYIIITDSE 158
>UniRef50_A3BK03 Cluster: Malic enzyme; n=2; Oryza sativa|Rep: Malic
enzyme - Oryza sativa subsp. japonica (Rice)
Length = 635
Score = 94.7 bits (225), Expect = 2e-18
Identities = 61/171 (35%), Positives = 87/171 (50%), Gaps = 10/171 (5%)
Frame = +2
Query: 11 ERDRIGLWGSGDGQPTSGLSGLDHLKHPGLNKGMAFTIEERQL------LGIHGLLPPRV 172
ERDR+GL G S +D P L F E + + L + P
Sbjct: 64 ERDRLGLRGLLPPNVVSSQQQIDRFT-PTLKYQYKFHSEIQHIDAKGLALNYLNISPAIC 122
Query: 173 KTQEEQVELCKLSIDRYEN--PLNKYIYLMGLLDRNEHLFYR--FVADNVAEMMPIVYTP 340
++ + V K + D + PL K+ L L DRNE ++Y+ + DN+ E PIVYTP
Sbjct: 123 ESSQYCVNADKYARDGPSDTYPLAKWRILNRLHDRNETMYYKAEVLIDNIEEHAPIVYTP 182
Query: 341 TVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVTDGER 493
TVGL CQ + ++RRPRG++ + D+G + ++ NWP V IVVTDG R
Sbjct: 183 TVGLVCQNYSGLFRRPRGMYFSAEDRGEMMSMVYNWPADQVDMIVVTDGSR 233
Score = 44.0 bits (99), Expect = 0.004
Identities = 18/42 (42%), Positives = 27/42 (64%)
Frame = +2
Query: 71 GLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVE 196
G D L P N+G F++ ER LG+ GLLPP V + ++Q++
Sbjct: 45 GSDILHDPWFNRGTGFSMTERDRLGLRGLLPPNVVSSQQQID 86
>UniRef50_Q48796 Cluster: Malolactic enzyme; n=49; Bacteria|Rep:
Malolactic enzyme - Oenococcus oeni (Leuconostoc oenos)
Length = 541
Score = 94.7 bits (225), Expect = 2e-18
Identities = 49/138 (35%), Positives = 79/138 (57%), Gaps = 2/138 (1%)
Frame = +2
Query: 83 LKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGL 262
L P +NKG AFT ER+ LG++GLLP +V+ +EQV+ + L K ++LM +
Sbjct: 8 LNDPFINKGTAFTEAEREELGLNGLLPAKVQALQEQVDQTYAQFQSKVSNLEKRLFLMEI 67
Query: 263 LDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRG-LFITIHDKGHVYDVL 439
+ N LFY+ + +V E MPIVY PT+ + + ++ P+G F+ I+ ++ L
Sbjct: 68 FNTNHVLFYKLFSQHVVEFMPIVYDPTIADTIENYSELFVEPQGAAFLDINHPENIQSTL 127
Query: 440 KNWPE-TDVRAIVVTDGE 490
KN D++ +VV+D E
Sbjct: 128 KNAANGRDIKLLVVSDAE 145
>UniRef50_P36013 Cluster: NAD-dependent malic enzyme, mitochondrial
precursor; n=15; Saccharomycetales|Rep: NAD-dependent
malic enzyme, mitochondrial precursor - Saccharomyces
cerevisiae (Baker's yeast)
Length = 669
Score = 90.2 bits (214), Expect = 4e-17
Identities = 49/143 (34%), Positives = 78/143 (54%), Gaps = 1/143 (0%)
Frame = +2
Query: 65 LSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKY 244
L L P NKG AFT EER+ + LLPP+V T +EQ+E + + PL K
Sbjct: 98 LESFQLLNSPLFNKGSAFTQEEREAFNLEALLPPQVNTLDEQLERSYKQLCYLKTPLAKN 157
Query: 245 IYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGH 424
++ L +N+ L++ + ++ E++PI+YTPT G A + +R+P G+F+ I +
Sbjct: 158 DFMTSLRVQNKVLYFALIRRHIKELVPIIYTPTEGDAIAAYSHRFRKPEGVFLDITEPDS 217
Query: 425 VYDVLKNW-PETDVRAIVVTDGE 490
+ L + + DV IVV+D E
Sbjct: 218 IECRLATYGGDKDVDYIVVSDSE 240
>UniRef50_Q9S4T5 Cluster: NAD-malate oxidoreductase homolog; n=15;
Legionellales|Rep: NAD-malate oxidoreductase homolog -
Legionella pneumophila
Length = 117
Score = 89.8 bits (213), Expect = 5e-17
Identities = 45/99 (45%), Positives = 63/99 (63%)
Frame = +2
Query: 59 SGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLN 238
+ L G L P LNKG AFT EER+ G+ G LP RV+T +EQV+ L Y L
Sbjct: 19 TSLCGKPLLTTPQLNKGTAFTQEERKDFGLLGKLPHRVETLDEQVKRAYLQYSSYTTRLQ 78
Query: 239 KYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLA 355
++IYL L D+N+ +FY+ ++ ++ EM+PI+YTP VG A
Sbjct: 79 QHIYLNNLHDKNQIVFYKLLSRHLGEMLPIIYTPIVGAA 117
>UniRef50_Q2HCG7 Cluster: Malic enzyme; n=1; Chaetomium
globosum|Rep: Malic enzyme - Chaetomium globosum (Soil
fungus)
Length = 586
Score = 86.2 bits (204), Expect = 7e-16
Identities = 52/149 (34%), Positives = 77/149 (51%), Gaps = 1/149 (0%)
Frame = +2
Query: 38 SGDGQPTSGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSID 217
S G L G L H NKG AFT EER+ + GLLP ++T ++QV+
Sbjct: 22 STSGPLECALKGTVLLNHSYFNKGSAFTKEERRDFELSGLLPQSIQTLDQQVQRAYEQYS 81
Query: 218 RYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGL 397
+ L K +L + ++NE L+++ M +VYTPT G A + F ++RRP+G+
Sbjct: 82 ARPDDLAKNTFLTSMKEQNEVLYFK--------MFSVVYTPTEGDAIENFSRLFRRPQGV 133
Query: 398 FITIHDKGHVYDVLKNW-PETDVRAIVVT 481
F+ +HD V+ L W D+ IVVT
Sbjct: 134 FLNVHDCDRVHHDLSLWGMPDDIDYIVVT 162
>UniRef50_A6XP71 Cluster: Malic enzyme protein 2; n=2;
Mucoromycotina|Rep: Malic enzyme protein 2 - Mortierella
alpina (Mortierella renispora)
Length = 669
Score = 84.6 bits (200), Expect = 2e-15
Identities = 50/155 (32%), Positives = 80/155 (51%), Gaps = 16/155 (10%)
Frame = +2
Query: 77 DHL----KHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKY 244
DHL +H G A E R L + GL P +V++ E Q + + + + KY
Sbjct: 95 DHLSNTHQHTDNMTGTATPTELRSALHLQGLTPAKVESFELQKKRALAQLRSKSSDIEKY 154
Query: 245 IYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVY------RRPRGLFIT 406
++L L + N LFY V D + E +P++YTPTVG ACQ + +Y +P GLF++
Sbjct: 155 VFLAWLRNTNVRLFYGLVGDQLEETLPLIYTPTVGTACQNYSSIYPFLAPPGQPDGLFLS 214
Query: 407 IHDKGHVYDVLKNW------PETDVRAIVVTDGER 493
I+D ++ +++N+ P + V+TDG R
Sbjct: 215 INDLPNLTQIIQNYKPFPQDPSLTPQIAVITDGSR 249
>UniRef50_Q5KEY3 Cluster: Malic enzyme; n=1; Filobasidiella
neoformans|Rep: Malic enzyme - Cryptococcus neoformans
(Filobasidiella neoformans)
Length = 629
Score = 83.8 bits (198), Expect = 4e-15
Identities = 52/160 (32%), Positives = 83/160 (51%), Gaps = 16/160 (10%)
Frame = +2
Query: 59 SGLSGLDHLKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLN 238
+ L G L P LNKG FT EERQ+ G+ G LP V + E+Q + + + +
Sbjct: 48 TNLRGSALLNTPRLNKGAGFTREERQIFGLEGFLPYDVHSLEKQCLRAYNQLCKQPSVIL 107
Query: 239 KYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHD- 415
K+ +L L D+N+ LFYR + D + E++ ++YTP A + ++RRP G +I+ +
Sbjct: 108 KHAFLASLRDQNQVLFYRLMQDRLKELLGVLYTPGAAEAVAGYSSLFRRPVGCYISFPNQ 167
Query: 416 -------KGHVYDVLK--------NWPETDVRAIVVTDGE 490
+GH+ DV + N P+ + +VVTD E
Sbjct: 168 DGMRAQLEGHLTDVNRTADVAYDSNKPDDAIDLVVVTDAE 207
>UniRef50_A4SKB8 Cluster: NAD-dependent malic enzyme; n=2;
Aeromonas|Rep: NAD-dependent malic enzyme - Aeromonas
salmonicida (strain A449)
Length = 516
Score = 81.0 bits (191), Expect = 3e-14
Identities = 41/129 (31%), Positives = 73/129 (56%)
Frame = +2
Query: 107 GMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGLLDRNEHLF 286
G + ER+ G+ G +P + ++ +Q + ++P ++++ L L + N LF
Sbjct: 7 GTSLPYAERKRQGLMGRMPHKEESLAQQRRRIYRLVSAMQSPFDQHLLLRQLQEDNPVLF 66
Query: 287 YRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVR 466
Y V ++ E++PI+YTP VG ACQ+ +Y R GL+++ HD+ + D + E +V
Sbjct: 67 YDLVRHHLPELLPIIYTPVVGEACQRHSDLYLRSHGLYLSWHDRDDL-DAIFAAVEQEVD 125
Query: 467 AIVVTDGER 493
IV++DGER
Sbjct: 126 VIVISDGER 134
>UniRef50_Q5K758 Cluster: Malic enzyme; n=1; Filobasidiella
neoformans|Rep: Malic enzyme - Cryptococcus neoformans
(Filobasidiella neoformans)
Length = 600
Score = 78.6 bits (185), Expect = 1e-13
Identities = 37/100 (37%), Positives = 58/100 (58%), Gaps = 3/100 (3%)
Frame = +2
Query: 203 KLSIDRYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYR 382
K + PL K+++L + + +LF+ + D + E+ PIVYTPTVG ACQK+ +Y
Sbjct: 95 KAHLQNLPTPLLKHVHLSKIRREDPNLFFSVMRDELTELAPIVYTPTVGEACQKYSQIYS 154
Query: 383 RPRGLFITIHDKGHVYDVLKNWPETDV---RAIVVTDGER 493
P GL++ I DK + ++L + V + +VVTDG R
Sbjct: 155 GPEGLYLNIEDKDRIPEILHQYASKLVAPPQILVVTDGSR 194
>UniRef50_Q5KBK5 Cluster: Nad-dependent malic enzyme, putative; n=2;
Filobasidiella neoformans|Rep: Nad-dependent malic
enzyme, putative - Cryptococcus neoformans
(Filobasidiella neoformans)
Length = 584
Score = 77.4 bits (182), Expect = 3e-13
Identities = 48/137 (35%), Positives = 69/137 (50%), Gaps = 1/137 (0%)
Frame = +2
Query: 83 LKHPGLNKGMAFTIEERQLLGIHGLLPPRVKTQEEQVELCKLSIDRYENPLNKYIYLMGL 262
L +P NKG AFT +ER + G LP V + E QV+ E + K +L +
Sbjct: 32 LNNPRFNKGSAFTHQERSEFALRGRLPYAVDSLEIQVKRAYEQYKSRETNILKNSFLQSM 91
Query: 263 LDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFIT-IHDKGHVYDVL 439
+N LFY + ++ EM PIVYTPT A + ++RR GL++T +K D L
Sbjct: 92 KAQNWTLFYALLQAHLVEMFPIVYTPTEADAIADYSHLFRRSEGLYLTPPGEKNMEEDFL 151
Query: 440 KNWPETDVRAIVVTDGE 490
++ IVV+DGE
Sbjct: 152 DACEGRELELIVVSDGE 168
>UniRef50_A3YYQ0 Cluster: Malate oxidoreductase; n=1; Synechococcus
sp. WH 5701|Rep: Malate oxidoreductase - Synechococcus
sp. WH 5701
Length = 517
Score = 72.5 bits (170), Expect = 9e-12
Identities = 40/108 (37%), Positives = 60/108 (55%), Gaps = 1/108 (0%)
Frame = +2
Query: 170 VKTQEEQVELCKLSIDRYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVG 349
+++ E QVE + N L ++ + + L N LF+RF+AD++ +MPIVYTPTVG
Sbjct: 1 MESLETQVERHWQAFLSLRNDLERFRFAVALRQANLTLFHRFLADHIEAVMPIVYTPTVG 60
Query: 350 LACQKFGLVYRRPR-GLFITIHDKGHVYDVLKNWPETDVRAIVVTDGE 490
A Q+F L YR P G+F+ D + VL V I++TD +
Sbjct: 61 AAIQRFSLDYRTPSGGVFLAAPDLERIESVLSQAATGPVDLILITDSQ 108
>UniRef50_A4RZU1 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Malic
enzyme - Ostreococcus lucimarinus CCE9901
Length = 539
Score = 60.9 bits (141), Expect = 3e-08
Identities = 28/67 (41%), Positives = 39/67 (58%)
Frame = +2
Query: 227 NPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFIT 406
+PL +Y+YL L + FYR + E+MP VYTPTVG AC+K+ + G++IT
Sbjct: 16 DPLERYVYLRELQRASAETFYRALVREPLELMPFVYTPTVGEACEKYHRLGIETNGVYIT 75
Query: 407 IHDKGHV 427
D G V
Sbjct: 76 ADDAGRV 82
>UniRef50_Q8S484 Cluster: Putative NADP-dependent malic enzyme; n=1;
Zea mays|Rep: Putative NADP-dependent malic enzyme - Zea
mays (Maize)
Length = 309
Score = 44.8 bits (101), Expect = 0.002
Identities = 28/74 (37%), Positives = 36/74 (48%)
Frame = +2
Query: 272 NEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDKGHVYDVLKNWP 451
+E LFY+ + DNV E++P VYT T G V DVLKNWP
Sbjct: 225 DERLFYKLLIDNVVELLPFVYTTT-------------------------GKVLDVLKNWP 259
Query: 452 ETDVRAIVVTDGER 493
+++ I VTD ER
Sbjct: 260 HRNIQVIFVTDSER 273
>UniRef50_UPI0000DB7FF6 Cluster: PREDICTED: hypothetical protein,
partial; n=1; Apis mellifera|Rep: PREDICTED:
hypothetical protein, partial - Apis mellifera
Length = 95
Score = 39.1 bits (87), Expect = 0.10
Identities = 17/33 (51%), Positives = 24/33 (72%)
Frame = +2
Query: 8 MERDRIGLWGSGDGQPTSGLSGLDHLKHPGLNK 106
++RD++G G GD ++ L GLDHLK+P LNK
Sbjct: 63 VQRDQLGQRGHGDAMCSNLLRGLDHLKNPRLNK 95
>UniRef50_Q116W6 Cluster: Putative uncharacterized protein; n=1;
Trichodesmium erythraeum IMS101|Rep: Putative
uncharacterized protein - Trichodesmium erythraeum
(strain IMS101)
Length = 1107
Score = 34.3 bits (75), Expect = 2.9
Identities = 27/88 (30%), Positives = 42/88 (47%), Gaps = 2/88 (2%)
Frame = +2
Query: 35 GSGDGQPTSGLSGLDHLKHPGL-NKGMAFTIEERQLLGIHGL-LPPRVKTQEEQVELCKL 208
GSG G +G + GL G++ E LLGI + LP ++K E + + +L
Sbjct: 609 GSGFGALGLKFTGCKIVDGFGLITLGISQNEFEINLLGIATMMLPTKLKDNYEPIAMAEL 668
Query: 209 SIDRYENPLNKYIYLMGLLDRNEHLFYR 292
+I NP+ I + G L N ++F R
Sbjct: 669 AIAGRFNPMEGVILIQGQLTENSYIFSR 696
>UniRef50_Q08PM3 Cluster: Hemin ABC transporter, periplasmic
hemin-binding protein; n=1; Stigmatella aurantiaca
DW4/3-1|Rep: Hemin ABC transporter, periplasmic
hemin-binding protein - Stigmatella aurantiaca DW4/3-1
Length = 332
Score = 34.3 bits (75), Expect = 2.9
Identities = 32/104 (30%), Positives = 44/104 (42%)
Frame = +2
Query: 176 TQEEQVELCKLSIDRYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLA 355
T EQ+ KL + EN + I GLL R E L F A+ + M T V
Sbjct: 143 TSAEQLRGAKLPVLILENSSKEGI--SGLLRRIEVLARVFNAEEAGQRMKQDITRQVAEL 200
Query: 356 CQKFGLVYRRPRGLFITIHDKGHVYDVLKNWPETDVRAIVVTDG 487
+K L ++PR LF+ H G + K ET A++ G
Sbjct: 201 EKKIALAKKKPRVLFLYAHSPGEAFVYGK---ETGTHALIELAG 241
>UniRef50_A1CKF3 Cluster: Stress response protein (Ish1), putative;
n=5; Pezizomycotina|Rep: Stress response protein (Ish1),
putative - Aspergillus clavatus
Length = 516
Score = 34.3 bits (75), Expect = 2.9
Identities = 24/64 (37%), Positives = 30/64 (46%), Gaps = 9/64 (14%)
Frame = -2
Query: 397 EAPW-SAVHKPELLTGQANRRSVHNWHHFSHVIRDETVEQML--------IAVQEPHKID 245
+A W S V KP GQA + HNWHH I D + L + V +P K D
Sbjct: 63 KANWDSKVQKP---LGQAAEHTTHNWHHAKEWIFDTWSDSQLKAFLDRHGVPVPQPRKRD 119
Query: 244 VLVK 233
VL+K
Sbjct: 120 VLLK 123
>UniRef50_Q4SHK3 Cluster: Chromosome 5 SCAF14581, whole genome
shotgun sequence; n=1; Tetraodon nigroviridis|Rep:
Chromosome 5 SCAF14581, whole genome shotgun sequence -
Tetraodon nigroviridis (Green puffer)
Length = 72
Score = 33.9 bits (74), Expect = 3.8
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = -3
Query: 204 LHSSTCSSCVLTRGGNNPCMPKS*RSSMVKAMPLLRPGCLRWSRPERPLV 55
L S SS +RG P P SS ++ R G RWSRP RP++
Sbjct: 22 LKGSAASSAAASRGQKTPASPTVPGSSQLQT----RSGQARWSRPRRPML 67
>UniRef50_Q4RNM6 Cluster: Chromosome 21 SCAF15012, whole genome
shotgun sequence; n=1; Tetraodon nigroviridis|Rep:
Chromosome 21 SCAF15012, whole genome shotgun sequence -
Tetraodon nigroviridis (Green puffer)
Length = 1187
Score = 33.5 bits (73), Expect = 5.0
Identities = 20/51 (39%), Positives = 26/51 (50%)
Frame = -3
Query: 222 YLSIESLHSSTCSSCVLTRGGNNPCMPKS*RSSMVKAMPLLRPGCLRWSRP 70
Y S+ES + T SC L G+ +P+S RSS L RP +WS P
Sbjct: 255 YNSLESSYQRTLQSC-LKSSGSVASLPQSDRSSSSSQESLNRPLTSKWSAP 304
>UniRef50_UPI0001560FE5 Cluster: PREDICTED: similar to KIAA2007
protein; n=2; Equus caballus|Rep: PREDICTED: similar to
KIAA2007 protein - Equus caballus
Length = 745
Score = 33.1 bits (72), Expect = 6.6
Identities = 19/49 (38%), Positives = 24/49 (48%)
Frame = -3
Query: 189 CSSCVLTRGGNNPCMPKS*RSSMVKAMPLLRPGCLRWSRPERPLVGCPS 43
C+S V + P+ RSS +PLLRP LR+S P P C S
Sbjct: 167 CASLVRASASPSRLFPRR-RSSSAVRVPLLRPLALRFSGPATPPCACES 214
>UniRef50_Q1EI20 Cluster: Putative uncharacterized protein; n=2;
root|Rep: Putative uncharacterized protein - uncultured
organism
Length = 302
Score = 32.7 bits (71), Expect = 8.8
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = +2
Query: 392 GLFITIHDKGHVYDVLKNWP 451
G+++TI + G + D+LKNWP
Sbjct: 210 GVYLTIREPGQITDILKNWP 229
>UniRef50_A0WC26 Cluster: Multi-sensor hybrid histidine kinase
precursor; n=2; Geobacter lovleyi SZ|Rep: Multi-sensor
hybrid histidine kinase precursor - Geobacter lovleyi SZ
Length = 1007
Score = 32.7 bits (71), Expect = 8.8
Identities = 18/48 (37%), Positives = 26/48 (54%), Gaps = 1/48 (2%)
Frame = +2
Query: 347 GLACQKFGLVYRRPRGL-FITIHDKGHVYDVLKNWPETDVRAIVVTDG 487
G+ K +YR R FI HD+ H+++ +KN PE + IV DG
Sbjct: 198 GIFDSKGVFLYRTARANEFIGKHDQPHLFEQMKNGPEEGIIDIVSNDG 245
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 699,142,256
Number of Sequences: 1657284
Number of extensions: 14509848
Number of successful extensions: 40294
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 38654
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 40274
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 54545459628
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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