BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P11_pT_C19
(664 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC17D1.02 |||diphthamide biosynthesis protein |Schizosaccharom... 108 8e-25
SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomy... 48 2e-06
SPCC757.02c |||epimarase |Schizosaccharomyces pombe|chr 3|||Manual 27 2.4
SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces pombe... 26 5.6
SPAC19D5.01 |pyp2||tyrosine phosphatase Pyp2|Schizosaccharomyces... 25 9.7
SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate... 25 9.7
>SPBC17D1.02 |||diphthamide biosynthesis protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 503
Score = 108 bits (259), Expect = 8e-25
Identities = 62/169 (36%), Positives = 103/169 (60%), Gaps = 7/169 (4%)
Frame = -1
Query: 652 KRRRFLIEKCKDANIIGILICKLSGKQTKDIISRMKELCKVNGKKSYIVSVGKPNSAKLA 473
+RR L+++C+DA +IGI+I L + ++++++++ GKK Y+++VGK N AKLA
Sbjct: 262 RRRYALVQRCRDAGVIGIVIGTLGVHRYLHVLNQLRKMILNAGKKPYMLAVGKLNPAKLA 321
Query: 472 NFPEIDIYVMIACPENDLYSSRDFYRPIVFPYELEIALNSNREPFPTYHVTDYDELLPGR 293
NF EI+ +V+IAC EN L S++FYRPIV P+EL AL+S+ + + +DE+L
Sbjct: 322 NFQEIECFVLIACGENSLIDSKEFYRPIVTPFELVKALSSDMS-WNNDFILSFDEVLKLS 380
Query: 292 RHYGDIKPIQEIAD------VSLITGR-IREAKVNHLDGSSQELAEQQN 167
+P + + + SLITG+ + + HLD + E A+ +N
Sbjct: 381 EGKQSKEPSEVLTEESAEPHFSLITGKFVNSTPMRHLD-VTLETADAKN 428
>SPBC3B8.05 |||diphthamide biosynthesis protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 436
Score = 47.6 bits (108), Expect = 2e-06
Identities = 23/93 (24%), Positives = 44/93 (47%)
Frame = -1
Query: 634 IEKCKDANIIGILICKLSGKQTKDIISRMKELCKVNGKKSYIVSVGKPNSAKLANFPEID 455
+EK + A G++ L + + ++ +K + N K V + + ++L F +ID
Sbjct: 302 VEKARTAKKFGLIQGTLGRQGSPKVLENLKNTLRKNNKDFVCVLMSEIFPSRLGQFSDID 361
Query: 454 IYVMIACPENDLYSSRDFYRPIVFPYELEIALN 356
++ +ACP + F P++ PYE A N
Sbjct: 362 AWIQVACPRLSIDWGYAFPAPLLTPYEASAAFN 394
>SPCC757.02c |||epimarase |Schizosaccharomyces pombe|chr 3|||Manual
Length = 405
Score = 27.1 bits (57), Expect = 2.4
Identities = 12/38 (31%), Positives = 22/38 (57%)
Frame = -2
Query: 372 LKLPSIPTENLFPHTMSQTMMNFYLEEDTMEILNRYKR 259
++LP I T+ P T S T +Y++ED ++ + K+
Sbjct: 135 VRLPMIETDIRVPETFSGTPNFYYVQEDILKEFSNGKK 172
>SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 377
Score = 25.8 bits (54), Expect = 5.6
Identities = 13/43 (30%), Positives = 21/43 (48%)
Frame = +1
Query: 286 SVFFQVEVHHSL*HGMWEKVLCWN*GQFQVRTGKLLVYKSPAN 414
+V V+VH+ L G W ++ W + KLL ++ AN
Sbjct: 244 NVPIMVQVHNFLNEGAWSEIKAWEREAGENTEPKLLRFEGNAN 286
>SPAC19D5.01 |pyp2||tyrosine phosphatase Pyp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 711
Score = 25.0 bits (52), Expect = 9.7
Identities = 12/35 (34%), Positives = 23/35 (65%)
Frame = +3
Query: 84 FLQFRLVFVLALSKSLSWRVFPIDSKAQFCCSASS 188
F +F+ + + +++SL++ DSK+ +CC ASS
Sbjct: 423 FYKFKRLEEMEMTRSLAFN----DSKSDWCCLASS 453
>SPAC3F10.06c |||initiator methionine tRNA 2'-O-ribosyl phosphate
transferase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 453
Score = 25.0 bits (52), Expect = 9.7
Identities = 11/25 (44%), Positives = 19/25 (76%)
Frame = +3
Query: 576 LPDSLHISIPIILASLHFSIRKRLR 650
+PD+L +IPI +A+L+ + +RLR
Sbjct: 111 MPDALSKTIPIWIATLNKCVFERLR 135
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,465,245
Number of Sequences: 5004
Number of extensions: 51259
Number of successful extensions: 146
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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