Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P11_F_N21
(667 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 165 4e-42
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 31 0.20
SPBC1347.04 |tim54||TIM22 inner membrane protein import complex ... 28 1.4
SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 27 2.4
SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pomb... 27 3.2
SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyc... 26 5.6
SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 26 5.6
SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|ch... 25 7.4
SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyce... 25 7.4
SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces ... 25 7.4
SPBC146.12 |coq6||monooxygenase Coq6|Schizosaccharomyces pombe|c... 25 9.8
SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr... 25 9.8
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 25 9.8
SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c... 25 9.8
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 165 bits (402), Expect = 4e-42
Identities = 76/181 (41%), Positives = 113/181 (62%)
Frame = +2
Query: 122 KVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSAFMRNAKIQSST 301
K+ IVG G VG AF++L + I ++D+ K +GE MDL H + ++
Sbjct: 22 KIVIVGAGNVGSTTAFTLLLSGLAAEIVIIDLNKKKAEGEAMDLNHAAPLSHETRVYLG- 80
Query: 302 DYSITAGSKICVVTAGVRQREGESRLDLVQRNTDVLKQIIPQLIKYSPDTILVIASNPVD 481
DY + V+TAG Q+ GE+R+DL++ N + K+I+ ++ KY+ D IL++A+NPVD
Sbjct: 81 DYKDCKDATAVVITAGKNQKPGETRMDLLKANISIFKEILREVTKYTKDAILLVATNPVD 140
Query: 482 ILTYVTWKISGLPKHRVIGSGTNLDSARFRYLLSDRLGIATTSCHGYIIGEHGDSSVPXW 661
+LTY T K++G P RVIGSGT +D+ARF+YL+ G+ S + IIGEHGDS + W
Sbjct: 141 VLTYATLKLTGFPAERVIGSGTIIDTARFQYLIGKLYGLDPQSVNADIIGEHGDSELAVW 200
Query: 662 S 664
S
Sbjct: 201 S 201
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 30.7 bits (66), Expect = 0.20
Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 1/74 (1%)
Frame = +2
Query: 95 HEKVDETWSKVTIVGVGQVGMAAAFSMLTQNVTNNIALVDMMADKLKGEMMDLQHGSA-F 271
H E+ S V +VG G VG+AA + + IA +D+ ADK E+ + G+ F
Sbjct: 187 HSAKVESGSTVAVVGCGCVGLAAMQGAVAAGASRIIA-IDINADK---EVYAKKFGATDF 242
Query: 272 MRNAKIQSSTDYSI 313
+ ++K++ Y I
Sbjct: 243 IDSSKVKDLVQYVI 256
>SPBC1347.04 |tim54||TIM22 inner membrane protein import complex
subunit Tim54|Schizosaccharomyces pombe|chr 2|||Manual
Length = 347
Score = 27.9 bits (59), Expect = 1.4
Identities = 12/48 (25%), Positives = 21/48 (43%)
Frame = -1
Query: 514 PANLPRNIRQNIHGVTGDHQYCVRTVLYQLRDYLFKHIGVSLHEIETR 371
P LPR ++ +HG GD Y R + +F + +E++
Sbjct: 56 PLELPRKLKVYLHGPPGDGIYVAREEFEEYIRPIFNAAAIEFETVESK 103
>SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit
Psm3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1194
Score = 27.1 bits (57), Expect = 2.4
Identities = 11/38 (28%), Positives = 21/38 (55%)
Frame = +2
Query: 224 DKLKGEMMDLQHGSAFMRNAKIQSSTDYSITAGSKICV 337
+++K E+ +L H +R K Q+ DY+ SK+ +
Sbjct: 268 ERIKAEITELNHSLELLRVEKQQNDEDYTNIMKSKVAL 305
>SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1073
Score = 26.6 bits (56), Expect = 3.2
Identities = 22/84 (26%), Positives = 36/84 (42%), Gaps = 1/84 (1%)
Frame = +2
Query: 407 LKQIIPQLIKYSPDTILVIASNPVDILTYVTWKISGLPKHRVIGSGTNLDSARFRYLLSD 586
L I L K + ++I+SN + YVT + G+P +V + + D SD
Sbjct: 611 LPHSISYLRKSNRAVYVLISSNSTLLTVYVTLTLEGVPDFKVYSNPISTDLPLTLQSPSD 670
Query: 587 RLG-IATTSCHGYIIGEHGDSSVP 655
I S + YI+G ++ P
Sbjct: 671 EFSTIYAWSDYLYIVGIDMETEQP 694
>SPAC821.09 |eng1||endo-1,3-beta-glucanase Eng1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1016
Score = 25.8 bits (54), Expect = 5.6
Identities = 12/40 (30%), Positives = 21/40 (52%)
Frame = -2
Query: 453 IVSGLYFISCGIICLSTSVFLCTRSRRDSPSRCRTPAVTT 334
I+ G+ ++SC C + S ++C+ SP+ T TT
Sbjct: 901 IIDGVDYLSCNGACYNPSQYVCSDGSL-SPNTVTTTKATT 939
>SPAPB1A10.07c |||sphingolipid biosynthesis
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 25.8 bits (54), Expect = 5.6
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = -1
Query: 271 EC*SVLQVHHLSFQFVSHHVY 209
+C SV+ VH LSF V H++
Sbjct: 87 KCYSVIAVHRLSFTLVMFHLF 107
>SPCC320.03 |||transcription factor |Schizosaccharomyces pombe|chr
3|||Manual
Length = 867
Score = 25.4 bits (53), Expect = 7.4
Identities = 9/45 (20%), Positives = 23/45 (51%)
Frame = +2
Query: 401 DVLKQIIPQLIKYSPDTILVIASNPVDILTYVTWKISGLPKHRVI 535
D + ++P + + V +++P + +Y T SG+ +H ++
Sbjct: 333 DRVSNVLPSITSFDSSVTTVPSNSPATLNSYTTSVPSGMSRHPML 377
>SPBC16A3.07c |nrm1||negative regulator of MBF|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 342
Score = 25.4 bits (53), Expect = 7.4
Identities = 20/72 (27%), Positives = 35/72 (48%), Gaps = 4/72 (5%)
Frame = -1
Query: 355 SNTSSNHADLRASCYRIIRTRLDLGV----PHEC*SVLQVHHLSFQFVSHHVY*SDVIRN 188
+NT++NH D C + +R RL+L + ++ S LQ + + H+ S+ + +
Sbjct: 120 TNTNNNHLDDIQCCAKNLRLRLELAMYKVQVNQTFSPLQDLPIVAKTKLHNCPNSEPVTS 179
Query: 187 ILRQHRESCGHP 152
I Q S G P
Sbjct: 180 IWNQRSLSSGKP 191
>SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 817
Score = 25.4 bits (53), Expect = 7.4
Identities = 15/40 (37%), Positives = 20/40 (50%), Gaps = 1/40 (2%)
Frame = +2
Query: 449 TILVIASNPVDILTYVTWKISGLPKHR-VIGSGTNLDSAR 565
TI I S P+D VTW + K+R V + N S+R
Sbjct: 644 TITNITSEPLDTPEQVTWLLDQASKNRSVAATNANEHSSR 683
>SPBC146.12 |coq6||monooxygenase Coq6|Schizosaccharomyces pombe|chr
2|||Manual
Length = 466
Score = 25.0 bits (52), Expect = 9.8
Identities = 16/48 (33%), Positives = 27/48 (56%), Gaps = 2/48 (4%)
Frame = +2
Query: 98 EKVDETWSKVTIVGVGQVGMAAAFSMLTQNVTNN--IALVDMMADKLK 235
+K+ + V IVG G VG+A A + + VT + + L+D+ D +K
Sbjct: 18 QKILQRQFDVVIVGSGPVGLALAAGLQSNPVTQSLKVGLLDIQ-DTMK 64
>SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 633
Score = 25.0 bits (52), Expect = 9.8
Identities = 12/43 (27%), Positives = 22/43 (51%)
Frame = +2
Query: 2 LVETSXHELADRLQRQAETRWSPXKKLFQPVHEKVDETWSKVT 130
LVET + A + + E W +KL++ + ++ T K+T
Sbjct: 160 LVETEAEKRAAITKEECEKSWLEQQKLYKDMEQENASTIQKLT 202
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.0 bits (52), Expect = 9.8
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = +2
Query: 599 ATTSCHGYIIGEHGDSSVPXWS 664
+ TSCHGY+I D V W+
Sbjct: 1194 SVTSCHGYLIA-FNDEFVEIWN 1214
>SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 25.0 bits (52), Expect = 9.8
Identities = 18/52 (34%), Positives = 30/52 (57%), Gaps = 3/52 (5%)
Frame = -2
Query: 480 STGLLAITNIVSGLYFISCGIICLSTSV---FLCTRSRRDSPSRCRTPAVTT 334
+TG A N++ L F GI+C++ V F TR+R +PS +T ++T+
Sbjct: 306 TTGHWAHANVLCYLIF---GILCIAGFVVNEFYTTRTRIITPSAFKTLSLTS 354
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,642,059
Number of Sequences: 5004
Number of extensions: 54693
Number of successful extensions: 170
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 169
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 303841898
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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