Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P11_F_I19
(813 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom... 193 2e-50
SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom... 160 2e-40
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 63 5e-11
SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase Le... 30 0.34
SPAC19G12.02c |pms1||MutL family mismatch-repair protein Pms1|Sc... 29 0.79
SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase... 28 1.4
SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase Lac... 27 2.4
SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Sp... 27 4.2
SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces ... 26 5.5
SPAC13D6.01 |pof14||F-box protein Pof14|Schizosaccharomyces pomb... 25 9.7
>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 356
Score = 193 bits (471), Expect = 2e-50
Identities = 99/188 (52%), Positives = 129/188 (68%), Gaps = 3/188 (1%)
Frame = +3
Query: 258 GRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEV---NPTLSAPLEDVVNSI 428
G+ TLIPGDG+G E AV E+FK A++P++FE + + N + L + + S+
Sbjct: 19 GKYTVTLIPGDGIGRETSNAVTEIFKTANVPIEFEEIDVTGMEKNNKSSGDALHEAIQSL 78
Query: 429 AVNKICIKGILATPDFSHTGELQTLNMKLRNALDLYANVVHVKSLPNVKCRHQDVDCIII 608
NK+ +KGIL TP F G + N+ LR LD+YA++V +K++P K RH +VD II
Sbjct: 79 KRNKVGLKGILFTP-FEKGGHT-SFNVALRKELDIYASLVLIKNIPGFKTRHDNVDFAII 136
Query: 609 REQTEGEYSALEHESVPGVVECLKIITAAKSERIAKFAFDYAVXMGRKKVTAVHKANIMX 788
RE TEGEYS LEH+SVPGVVE LKIIT KS+RIA+FAFD+A+ GRK VT +HKANIM
Sbjct: 137 RENTEGEYSGLEHQSVPGVVESLKIITEYKSKRIAQFAFDFALQNGRKSVTCIHKANIMK 196
Query: 789 LGDGLFLR 812
L DGLF R
Sbjct: 197 LADGLFRR 204
>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 160 bits (389), Expect = 2e-40
Identities = 82/199 (41%), Positives = 121/199 (60%), Gaps = 2/199 (1%)
Frame = +3
Query: 219 APFGALQPRATKEGRIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFESFFFSEV--NPT 392
A G + G T+I GDG+GPE+ +V+ +FKAA +P+++E + N T
Sbjct: 31 AAAGTYEGVKNANGNYTVTMIAGDGIGPEIAQSVERIFKAAKVPIEWERVKVYPILKNGT 90
Query: 393 LSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKLRNALDLYANVVHVKSLPNV 572
+ P +D S+ NK+ +KG LATP G + ++N+ LR L+ANV S+
Sbjct: 91 TTIP-DDAKESVRKNKVALKGPLATP--IGKGHV-SMNLTLRRTFGLFANVRPCVSITGY 146
Query: 573 KCRHQDVDCIIIREQTEGEYSALEHESVPGVVECLKIITAAKSERIAKFAFDYAVXMGRK 752
K + +V+ ++IRE TEGEYS +EHE +PGVV+ +K+IT A SER+ ++AF YA G+
Sbjct: 147 KTPYDNVNTVLIRENTEGEYSGIEHEVIPGVVQSIKLITRAASERVIRYAFQYARQTGKN 206
Query: 753 KVTAVHKANIMXLGDGLFL 809
+T VHKA IM + DGLFL
Sbjct: 207 NITVVHKATIMRMADGLFL 225
>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 362
Score = 62.9 bits (146), Expect = 5e-11
Identities = 65/207 (31%), Positives = 98/207 (47%), Gaps = 25/207 (12%)
Frame = +3
Query: 261 RIKCTLIPGDGVGPELVYAVQEVFKAASIPVDFE-SFFFSEVNP-------TLSAPLEDV 416
RI LIP DG+G E+V A + + + ++P + F F +++ T A E
Sbjct: 6 RIVLGLIPADGIGKEVVPAARRLME--NLPAKHKLKFDFIDLDAGWGTFERTGKALPERT 63
Query: 417 VNSIAVN-KICIKGILATPDFSHTGELQTLNMKLRNALDLYANVVHVKSLPNVKCRHQDV 593
V + + G + +P G + + LR + LYANV VKSL K + V
Sbjct: 64 VERLKTECNAALFGAVQSPTHKVAGYSSPI-VALRKKMGLYANVRPVKSLDGAKGK--PV 120
Query: 594 DCIIIREQTEGEYSALEH--ESVPG--VVECLKIITAAKSERIAKFAFDYAVXMGR---- 749
D +I+RE TE Y E ++ PG V E ++ I+ S +I K AF+ A +
Sbjct: 121 DLVIVRENTECLYVKEERMVQNTPGKRVAEAIRRISEEASTKIGKMAFEIAKSRQKIRES 180
Query: 750 ------KK--VTAVHKANIMXLGDGLF 806
KK VT +HK+N+M + DGLF
Sbjct: 181 GTYSIHKKPLVTIIHKSNVMSVTDGLF 207
>SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase
Leu1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 371
Score = 30.3 bits (65), Expect = 0.34
Identities = 24/101 (23%), Positives = 47/101 (46%), Gaps = 8/101 (7%)
Frame = +3
Query: 267 KCTLIPGDGVGPELVYAVQEVFKAA-----SIPVDFESFFFSEVN-PTLSAPLED-VVNS 425
K ++PGD +GPE+V + EV K + ++FE + PL D V +
Sbjct: 5 KIVVLPGDHIGPEIVASALEVLKVVEKKRPELKLEFEEHKIGGASIDAYGTPLTDETVKA 64
Query: 426 IAVNKICIKGILATPDFSHTG-ELQTLNMKLRNALDLYANV 545
+ G + P++++ + +KLR ++ ++AN+
Sbjct: 65 CLEADGVLLGAVGGPEWTNPNCRPEQGLLKLRKSMGVWANL 105
>SPAC19G12.02c |pms1||MutL family mismatch-repair protein
Pms1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 794
Score = 29.1 bits (62), Expect = 0.79
Identities = 25/83 (30%), Positives = 39/83 (46%)
Frame = +3
Query: 300 PELVYAVQEVFKAASIPVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFS 479
P A+Q++ S P+D + F ++N +LS +D+V S A+ K K I D S
Sbjct: 533 PSKTAALQKLKFFQSRPLDGLNKFSKKINISLSGVQKDIVRSDALLKFSNK-IGVVHDIS 591
Query: 480 HTGELQTLNMKLRNALDLYANVV 548
+ LN+ + A L VV
Sbjct: 592 DENQEDHLNLTVHKADFLRMRVV 614
>SPBPJ4664.06 |gpt1||UDP-glucose-glycoprotein glucosyltransferase
Gpt1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1448
Score = 28.3 bits (60), Expect = 1.4
Identities = 16/70 (22%), Positives = 31/70 (44%)
Frame = +3
Query: 348 PVDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKLRNAL 527
P F S N L D + ++ +KI I +LAT + + ++ T +L
Sbjct: 245 PASFTSSRNKRSNERLFGMTSDSLQTVTPDKIAILDLLATQSIASSTDMLTAFRELTQDF 304
Query: 528 DLYANVVHVK 557
+YA+ + ++
Sbjct: 305 PIYAHYLSIQ 314
>SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase
Lac1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 384
Score = 27.5 bits (58), Expect = 2.4
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = -2
Query: 443 NLVNSDRVYDVFEWCTKCWIHFRKEKGFKVHW 348
N VN+ VY +F WI+ R FK+ W
Sbjct: 269 NYVNTVIVYPIFVIFVFVWIYMRHYLNFKIMW 300
>SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor
Spt6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1365
Score = 26.6 bits (56), Expect = 4.2
Identities = 11/37 (29%), Positives = 24/37 (64%), Gaps = 1/37 (2%)
Frame = -2
Query: 446 TNLVNSDRVYDVFEWCTKCW-IHFRKEKGFKVHWNAG 339
T L++ + ++++F CTK W +H +K+ K++ + G
Sbjct: 355 TPLLSQNDLWNIFFLCTKFWSLHSKKQDILKLYSDLG 391
>SPCC622.13c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1098
Score = 26.2 bits (55), Expect = 5.5
Identities = 16/54 (29%), Positives = 24/54 (44%)
Frame = +3
Query: 375 SEVNPTLSAPLEDVVNSIAVNKICIKGILATPDFSHTGELQTLNMKLRNALDLY 536
S++N A LED N +C++ +A S LQ +K R+ L Y
Sbjct: 541 SQLNVFTKASLEDKQKKEKYNLLCVRSCIAIDSISWISSLQ--GVKFRSKLMAY 592
>SPAC13D6.01 |pof14||F-box protein Pof14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 431
Score = 25.4 bits (53), Expect = 9.7
Identities = 17/61 (27%), Positives = 30/61 (49%), Gaps = 3/61 (4%)
Frame = -3
Query: 778 LALWTAVTFLRPXFTA*SKANFA---IRSDFAAVMIFKHSTTPGTDSCSKAEYSPSVCSL 608
+A +T + + P F S ++A I SD +++ T GT SC ++ Y C+L
Sbjct: 255 VAKFTDIPTIIPIFLDPSYQSYAAKTIHSDTSSLASSIIQTGDGTQSCDESTYIELACNL 314
Query: 607 M 605
+
Sbjct: 315 V 315
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,438,160
Number of Sequences: 5004
Number of extensions: 76504
Number of successful extensions: 220
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 210
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 214
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 396433620
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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