Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P11_F_I13
(719 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 245 4e-66
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 93 4e-20
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 55 9e-09
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 42 7e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 36 0.004
SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 31 0.17
SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccha... 29 0.51
SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4 |S... 27 2.7
SPBC2D10.18 |abc1|coq8|ABC1 kinase family protein|Schizosaccharo... 26 4.7
SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 26 4.7
SPBP22H7.08 |rps1002|rps10-2, rps10B|40S ribosomal protein S10|S... 26 4.7
SPBC30D10.15 |||snoRNP assembly factor |Schizosaccharomyces pomb... 26 6.2
SPBC1105.07c |||nuclear pore associated protein Thp1-Sac3 comple... 26 6.2
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 25 8.2
SPAC14C4.07 |||membrane transporter|Schizosaccharomyces pombe|ch... 25 8.2
SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pom... 25 8.2
SPAC1071.01c |pta1|SPAC4H3.15c|mRNA cleavage and polyadenylation... 25 8.2
SPAC21E11.05c |cyp8||cyclophilin family peptidyl-prolyl cis-tran... 25 8.2
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 245 bits (600), Expect = 4e-66
Identities = 110/168 (65%), Positives = 133/168 (79%)
Frame = +3
Query: 201 MGKQNSKLKPEVLEDLKQNTEFSDAEIQEWYKGFLKDCPSGHLSVEEFKKIYGNFFPYGD 380
MGK SKL + L+DL ++T F E+Q+WYKGF KDCPSGHL+ EF+KIY FFP+GD
Sbjct: 1 MGKSQSKLSQDQLQDLVRSTRFDKKELQQWYKGFFKDCPSGHLNKSEFQKIYKQFFPFGD 60
Query: 381 ASKFAEHVFRTFDANGDGTIDFREFLCALSVTSRGKLEQKLKWAFSMYDLDGNGYISRQE 560
S FAE+VF FDA+ +G IDF+EF+CALSVTSRG+L KL WAF +YDLD NG IS E
Sbjct: 61 PSAFAEYVFNVFDADKNGYIDFKEFICALSVTSRGELNDKLIWAFQLYDLDNNGLISYDE 120
Query: 561 MLEIVTAIYKMVGSVMKMPEDESTPEKRTDKIFRQMDRNKDGKLSLEE 704
ML IV AIYKMVGS++K+PEDE TPEKR +KIF MD+NKDG+L+LEE
Sbjct: 121 MLRIVDAIYKMVGSMVKLPEDEDTPEKRVNKIFNMMDKNKDGQLTLEE 168
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 93.1 bits (221), Expect = 4e-20
Identities = 60/161 (37%), Positives = 95/161 (59%), Gaps = 3/161 (1%)
Frame = +3
Query: 231 EVLEDLKQNTEFSDAEIQEWYKGFLK-DC-PSGHLSVEEFKKIYGNFFPYGDASKFAEHV 404
++ EDL N+ FS+ EI+ K F+K D SG + EF I P ++ A +
Sbjct: 7 QIFEDLISNSSFSNEEIERIRKRFIKIDANQSGSIDRNEFLSI-----PSVASNPLASRL 61
Query: 405 FRTFDANGDGTIDFREFLCALSVTS-RGKLEQKLKWAFSMYDLDGNGYISRQEMLEIVTA 581
F D +G G +DF+EF+ +LSV S G E+KLK+AF +YD+D +GYIS E+ +
Sbjct: 62 FSVVDEDGGGDVDFQEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLV--- 118
Query: 582 IYKMVGSVMKMPEDESTPEKRTDKIFRQMDRNKDGKLSLEE 704
+ MVG+ ++ ED+ ++ DK ++D+++DGK+S EE
Sbjct: 119 LKMMVGTNLR--EDQL--QQIVDKTIMEVDKDRDGKISFEE 155
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 55.2 bits (127), Expect = 9e-09
Identities = 39/150 (26%), Positives = 77/150 (51%), Gaps = 1/150 (0%)
Frame = +3
Query: 258 TEFSDAEIQEWYKGFLKDCPSGHLSVEEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGT 437
T+ AE +E + F +D G+++ E + + A++ + + DA+G+GT
Sbjct: 7 TDEQIAEFREAFSLFDRD-QDGNITSNELGVVMRSLGQSPTAAELQDMI-NEVDADGNGT 64
Query: 438 IDFREFLCALSVTSRGK-LEQKLKWAFSMYDLDGNGYISRQEMLEIVTAIYKMVGSVMKM 614
IDF EFL ++ + E++++ AF ++D DGNGYI+ +E+ ++T++
Sbjct: 65 IDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSL---------- 114
Query: 615 PEDESTPEKRTDKIFRQMDRNKDGKLSLEE 704
E ++ + R+ D + DG ++ EE
Sbjct: 115 --GERLSQEEVADMIREADTDGDGVINYEE 142
Score = 32.7 bits (71), Expect = 0.054
Identities = 22/103 (21%), Positives = 43/103 (41%)
Frame = +3
Query: 273 AEIQEWYKGFLKDCPSGHLSVEEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFRE 452
AE+Q+ D +G + EF + D + F+ FD +G+G I E
Sbjct: 48 AELQDMINEVDAD-GNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEE 106
Query: 453 FLCALSVTSRGKLEQKLKWAFSMYDLDGNGYISRQEMLEIVTA 581
L+ ++++ D DG+G I+ +E ++++
Sbjct: 107 LTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEFSRVISS 149
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 42.3 bits (95), Expect = 7e-05
Identities = 15/69 (21%), Positives = 40/69 (57%)
Frame = +3
Query: 378 DASKFAEHVFRTFDANGDGTIDFREFLCALSVTSRGKLEQKLKWAFSMYDLDGNGYISRQ 557
++S F H+F FD + G++ ++ + ++ + + + + F +YD +G+G++ +
Sbjct: 576 NSSSFLRHLFLRFDKSMTGSLSLQDLVSGIAELKFRDVMRNISFIFELYDFNGDGFMDKP 635
Query: 558 EMLEIVTAI 584
++L++ AI
Sbjct: 636 DVLKVSEAI 644
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 36.3 bits (80), Expect = 0.004
Identities = 21/85 (24%), Positives = 38/85 (44%)
Frame = +3
Query: 321 GHLSVEEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFLCALSVTSRGKLEQK 500
G+L +E+F ++ D + + F FD + G I R + +Q+
Sbjct: 88 GYLQMEDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQE 147
Query: 501 LKWAFSMYDLDGNGYISRQEMLEIV 575
L+ +DLD +G I+ QE + I+
Sbjct: 148 LEAMIEEFDLDQDGEINEQEFIAIM 172
Score = 35.1 bits (77), Expect = 0.010
Identities = 23/98 (23%), Positives = 44/98 (44%)
Frame = +3
Query: 405 FRTFDANGDGTIDFREFLCALSVTSRGKLEQKLKWAFSMYDLDGNGYISRQEMLEIVTAI 584
F+ FD++ D ID+ E A+ + ++ +D G GY+ ++ + ++T
Sbjct: 43 FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTE- 101
Query: 585 YKMVGSVMKMPEDESTPEKRTDKIFRQMDRNKDGKLSL 698
K+V E P + + F D ++ GK+SL
Sbjct: 102 -KIV---------ERDPLEEIKRAFELFDDDETGKISL 129
>SPAC1687.14c |||EF hand family protein, unknown
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 31.1 bits (67), Expect = 0.17
Identities = 20/61 (32%), Positives = 30/61 (49%), Gaps = 1/61 (1%)
Frame = +3
Query: 393 AEHVFRTFDANGDGTIDFREFLCALSVTSRGKLEQKLKWAFSMYDLDG-NGYISRQEMLE 569
AE F FD G IDF + + + +++L+ M DL G NG +SR+E E
Sbjct: 14 AEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQ---LMLDLAGTNGKVSREEFAE 70
Query: 570 I 572
+
Sbjct: 71 L 71
>SPCC1322.14c |vtc4||vacuolar transporter chaperone
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 721
Score = 29.5 bits (63), Expect = 0.51
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +1
Query: 166 KLQVQSGNNPE*WVNKIVNS 225
KLQ Q G +P WVN +VNS
Sbjct: 423 KLQTQFGQDPPEWVNNLVNS 442
>SPCC31H12.08c |ccr4|SPCC5E4.02c|CCR4-Not complex subunit Ccr4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 690
Score = 27.1 bits (57), Expect = 2.7
Identities = 29/121 (23%), Positives = 45/121 (37%), Gaps = 1/121 (0%)
Frame = -2
Query: 433 PSPFASNVRNTC-SANLLASP*GKKFPYIFLNSSTERWPLGQSFRNPLYHSCISASENSV 257
PS + N++ T S N+L +++ L T W L S+R L + +
Sbjct: 327 PSTTSKNLKFTIMSYNVLC----ERYATSTLYGYTPSWALSWSYRKDLIMQELGGYNADI 382
Query: 256 FCFRSSNTSGLSLLFCLPIILDYYRFALVVFGYEFTIRTNTTESRLVDQVFCPLFFEPCL 77
C + + F + L Y+ V F N E R+VD C FF+
Sbjct: 383 ICLQEVDVENYDTFFAPQMSLKGYKG--VHFPKSRVRTMNEVERRIVDG--CATFFKTSK 438
Query: 76 Y 74
Y
Sbjct: 439 Y 439
>SPBC2D10.18 |abc1|coq8|ABC1 kinase family
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 610
Score = 26.2 bits (55), Expect = 4.7
Identities = 18/82 (21%), Positives = 42/82 (51%), Gaps = 2/82 (2%)
Frame = +3
Query: 477 SRGKLEQKLKWAFSMYDLDGNGYISRQEMLEIVTAIYKMVGSVMKMPEDESTPEKR--TD 650
S G + +K+K + + DG ++ + + +V + +M G+ +KM + S + +
Sbjct: 198 SVGAIGEKMKRMWGISKDDGALLLNERNVEILVNKLTQMRGAALKMGQMLSFQDSKLIDP 257
Query: 651 KIFRQMDRNKDGKLSLEESXKE 716
++ + ++R +DG S+ E E
Sbjct: 258 RVSQILERVRDGAHSMPEKQLE 279
>SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 26.2 bits (55), Expect = 4.7
Identities = 8/20 (40%), Positives = 13/20 (65%)
Frame = +3
Query: 303 LKDCPSGHLSVEEFKKIYGN 362
L P+G +EE+K++Y N
Sbjct: 255 LASAPNGSFDIEEYKRVYSN 274
>SPBP22H7.08 |rps1002|rps10-2, rps10B|40S ribosomal protein
S10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 26.2 bits (55), Expect = 4.7
Identities = 21/80 (26%), Positives = 34/80 (42%), Gaps = 7/80 (8%)
Frame = +3
Query: 468 SVTSRGKLEQKLKWAFSMYDLDGNGYISRQEML----EIVTAIYKMVGSVMKMPEDESTP 635
S+ SRG L+ + W + Y L G +E L E+V A +K P
Sbjct: 48 SLDSRGYLKTRYNWGWFYYTLTNEGVEYLREYLHLPAEVVPATHKRQVRPAAPRAGRPEP 107
Query: 636 EKRT---DKIFRQMDRNKDG 686
+R+ D +R+ ++ DG
Sbjct: 108 RERSSAADAGYRRAEKKDDG 127
>SPBC30D10.15 |||snoRNP assembly factor |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 516
Score = 25.8 bits (54), Expect = 6.2
Identities = 9/29 (31%), Positives = 18/29 (62%)
Frame = +3
Query: 201 MGKQNSKLKPEVLEDLKQNTEFSDAEIQE 287
+GK ++ L + D + NT FS+ +++E
Sbjct: 180 IGKSSADLSSDSSSDSESNTSFSETDVEE 208
>SPBC1105.07c |||nuclear pore associated protein Thp1-Sac3 complex
subunit |Schizosaccharomyces pombe|chr 2|||Manual
Length = 442
Score = 25.8 bits (54), Expect = 6.2
Identities = 12/43 (27%), Positives = 22/43 (51%)
Frame = +3
Query: 531 DGNGYISRQEMLEIVTAIYKMVGSVMKMPEDESTPEKRTDKIF 659
DGNG+I R+ + + + + S++ + P K+ D IF
Sbjct: 141 DGNGFIQRKYVSDASRNVLRTFNSILSDRQQNINPSKK-DAIF 182
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 25.4 bits (53), Expect = 8.2
Identities = 17/55 (30%), Positives = 29/55 (52%)
Frame = -2
Query: 346 LNSSTERWPLGQSFRNPLYHSCISASENSVFCFRSSNTSGLSLLFCLPIILDYYR 182
L++S + W L Q F + L + C+ +S NS + ++ SLL CL + Y +
Sbjct: 154 LSNSNDSW-LWQRFSSLLLN-CLVSSINSHRIEGTDTSAETSLLHCLAYVAPYLK 206
>SPAC14C4.07 |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 644
Score = 25.4 bits (53), Expect = 8.2
Identities = 13/33 (39%), Positives = 15/33 (45%)
Frame = -2
Query: 133 TESRLVDQVFCPLFFEPCLYPCCVITKRFIRLY 35
TES V F +Y CC I F+RLY
Sbjct: 605 TESMKVSYPNMIFFLSAVIYGCCFIIIFFMRLY 637
>SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 166
Score = 25.4 bits (53), Expect = 8.2
Identities = 13/23 (56%), Positives = 16/23 (69%), Gaps = 1/23 (4%)
Frame = -2
Query: 454 NSLKSI-VPSPFASNVRNTCSAN 389
NS SI +P+PF+S VRN AN
Sbjct: 53 NSFVSIHMPAPFSSVVRNALKAN 75
>SPAC1071.01c |pta1|SPAC4H3.15c|mRNA cleavage and polyadenylation
specificity factor complex subunit
Pta1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.4 bits (53), Expect = 8.2
Identities = 10/38 (26%), Positives = 20/38 (52%)
Frame = +3
Query: 192 SRIMGKQNSKLKPEVLEDLKQNTEFSDAEIQEWYKGFL 305
+R + K + L PE++ ++ +SD ++W FL
Sbjct: 16 ARDIVKTDQSLFPEIVRNILSVANYSDIRYKKWMANFL 53
>SPAC21E11.05c |cyp8||cyclophilin family peptidyl-prolyl cis-trans
isomerase Cyp8|Schizosaccharomyces pombe|chr 1|||Manual
Length = 516
Score = 25.4 bits (53), Expect = 8.2
Identities = 8/29 (27%), Positives = 17/29 (58%)
Frame = -2
Query: 145 RTNTTESRLVDQVFCPLFFEPCLYPCCVI 59
+ +TT + + +C L +P +PCC++
Sbjct: 31 KNSTTSYKQLPFNYCSLSLQPFNHPCCLV 59
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,277,326
Number of Sequences: 5004
Number of extensions: 75658
Number of successful extensions: 246
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 231
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 241
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 337208592
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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