Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P11_F_B16
(888 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr... 29 0.88
SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyc... 28 1.5
SPAC4F10.07c |atg13|apg13, mug78|autophagy associated protein At... 27 2.7
SPBC1105.02c |lys4||homocitrate synthase |Schizosaccharomyces po... 27 3.6
SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomy... 27 4.7
SPCC1442.09 |trp3||anthranilate synthase component I|Schizosacch... 26 6.2
SPAC26H5.12 |rpo41||mitochondrial DNA-directed RNA polymerase|Sc... 26 6.2
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 8.2
SPCC306.04c |set1||histone lysine methyltransferase Set1|Schizos... 26 8.2
SPBC32F12.02 |rec14||recombination protein Rec14|Schizosaccharom... 26 8.2
SPAPB1A10.12c |alo1||D-arabinono-1,4-lactone oxidase|Schizosacch... 26 8.2
>SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 652
Score = 29.1 bits (62), Expect = 0.88
Identities = 34/124 (27%), Positives = 54/124 (43%), Gaps = 10/124 (8%)
Frame = +3
Query: 516 ITIKDFEHFLDHRTIVNEETDPIFG--RNLFSLKGQEW----KDM-RSTLS-PAFTSSKM 671
+TI+ +H RT +P FG R L+ L + +D+ R LS PA +
Sbjct: 479 VTIEKRKHTEHIRTYTPNVIEPSFGLGRILYVLMEHAYWTRPEDVNRGVLSFPASIAPIK 538
Query: 672 KLMMPLIVEVG-EQMINALKKNIKNSGVGY-VDIDTKDLTTRYANDVIASCAFGLKVDSL 845
L++PL + L ++N G+ +D ++ RYA + FGL VD
Sbjct: 539 ALIVPLSRNAEFAPFVKKLSAKLRNLGISNKIDDSNANIGRRYARNDELGTPFGLTVDFE 598
Query: 846 TEEN 857
T +N
Sbjct: 599 TLQN 602
>SPBP8B7.27 |mug30||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 807
Score = 28.3 bits (60), Expect = 1.5
Identities = 16/47 (34%), Positives = 22/47 (46%)
Frame = -3
Query: 415 KWLILKTILPMLPSMGSGVMLLISCLLK*QYICFRYSKRAKPITNVH 275
+WL+L T P G L ++C Y+ F YS R+K I H
Sbjct: 479 EWLLLLTRKVFSPEFG----LFVNCEESSNYLWFNYSHRSKEIDYYH 521
>SPAC4F10.07c |atg13|apg13, mug78|autophagy associated protein Atg13
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 758
Score = 27.5 bits (58), Expect = 2.7
Identities = 14/32 (43%), Positives = 21/32 (65%)
Frame = +1
Query: 7 LPLIRVASKPLSSLSLNISKXQSPVCARTSIS 102
+P SK L+SL ++ K QSP+CAR++ S
Sbjct: 621 VPSANDTSKQLASLH-DMRKSQSPICARSATS 651
>SPBC1105.02c |lys4||homocitrate synthase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 418
Score = 27.1 bits (57), Expect = 3.6
Identities = 13/42 (30%), Positives = 21/42 (50%)
Frame = +3
Query: 723 LKKNIKNSGVGYVDIDTKDLTTRYANDVIASCAFGLKVDSLT 848
+ K + N GV Y+++ + + + D A C GLK LT
Sbjct: 61 IAKALDNFGVDYIELTSPVASEQSRQDCEAICKLGLKCKILT 102
>SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1201
Score = 26.6 bits (56), Expect = 4.7
Identities = 15/66 (22%), Positives = 32/66 (48%)
Frame = +3
Query: 390 KIVFKINHFVDDISQLYNKFPEERYEFVNPVIYIRDIEIVKRITIKDFEHFLDHRTIVNE 569
KI K++ ++ + Y KF + + + I + +VK + +FE+FL T+ +
Sbjct: 901 KIQVKLSSILEMLRSRYKKFKKPIIDLNDIYIDESNERVVKGKSKDNFEYFLSGNTVTRK 960
Query: 570 ETDPIF 587
+ + F
Sbjct: 961 KDNACF 966
>SPCC1442.09 |trp3||anthranilate synthase component
I|Schizosaccharomyces pombe|chr 3|||Manual
Length = 489
Score = 26.2 bits (55), Expect = 6.2
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = -3
Query: 676 SFILELVKAGDKVDRISFHSWPFK 605
SFILE V G+ V R SF P++
Sbjct: 49 SFILESVTQGESVSRYSFIGSPYR 72
>SPAC26H5.12 |rpo41||mitochondrial DNA-directed RNA
polymerase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1120
Score = 26.2 bits (55), Expect = 6.2
Identities = 11/25 (44%), Positives = 17/25 (68%)
Frame = -2
Query: 461 SLFWKFVVQLRNIINKMVNLEDDLA 387
SLF+ + VQL + + ++L DDLA
Sbjct: 291 SLFYSWYVQLEQLFKEEISLIDDLA 315
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.8 bits (54), Expect = 8.2
Identities = 19/76 (25%), Positives = 36/76 (47%)
Frame = +3
Query: 654 FTSSKMKLMMPLIVEVGEQMINALKKNIKNSGVGYVDIDTKDLTTRYANDVIASCAFGLK 833
F K++ P+I ++ + I +LKK I + ++ K L ANDV + + +
Sbjct: 979 FKEEKIESAPPIINDIDSE-IASLKKRIHEKSLVVNALEDKKLAATPANDV-QNDSLIYR 1036
Query: 834 VDSLTEENNQFYAMGK 881
+ S+ +E N+ K
Sbjct: 1037 IKSVQDEINRLSTSNK 1052
>SPCC306.04c |set1||histone lysine methyltransferase
Set1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 920
Score = 25.8 bits (54), Expect = 8.2
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +2
Query: 230 SYQTEQKKRYNDYHYMDISDRF 295
S +T+ K NDY Y+ I DRF
Sbjct: 262 SSETDVPKNINDYIYLLIDDRF 283
>SPBC32F12.02 |rec14||recombination protein
Rec14|Schizosaccharomyces pombe|chr 2|||Manual
Length = 302
Score = 25.8 bits (54), Expect = 8.2
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +3
Query: 780 LTTRYANDVIASCAFGLKVDSLTEENNQFYAMG 878
L T +V+ SC FG V E N+F +G
Sbjct: 65 LATSLDENVVVSCGFGQDVYVWNPETNEFRDLG 97
>SPAPB1A10.12c |alo1||D-arabinono-1,4-lactone
oxidase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 461
Score = 25.8 bits (54), Expect = 8.2
Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 2/49 (4%)
Frame = +3
Query: 318 QIYCYFSKHEIKSITPLPILGNMGKIVFKINHFVDDISQL--YNKFPEE 458
Q++CYFS+H S +P+ + IN+ VDD ++ Y +P E
Sbjct: 312 QMFCYFSQH--VSEWGIPLESAPDALEKLINYTVDDAGKIGAYTHWPIE 358
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,252,924
Number of Sequences: 5004
Number of extensions: 66212
Number of successful extensions: 223
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 219
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 223
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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