Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P10_pT_N23
(710 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 57 3e-09
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 44 2e-05
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 43 4e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 30 0.38
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.5
SPAC22F8.07c |rtf1||replication termination factor Rtf1|Schizosa... 26 4.6
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 6.1
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 56.8 bits (131), Expect = 3e-09
Identities = 29/75 (38%), Positives = 44/75 (58%)
Frame = -1
Query: 611 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 432
A+K KD E+ E K++DK+ NG + ELTH L +LGE+L EVA++ ++
Sbjct: 75 ARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA--DT 132
Query: 431 DDDGMIPYAAFLKKV 387
D DG+I Y F + +
Sbjct: 133 DGDGVINYEEFSRVI 147
Score = 29.1 bits (62), Expect = 0.66
Identities = 15/61 (24%), Positives = 32/61 (52%)
Frame = -1
Query: 578 DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 399
+F E L+D++++G + EL + +LG+ +E+ ++ + D +G I + F
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEF 70
Query: 398 L 396
L
Sbjct: 71 L 71
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 44.0 bits (99), Expect = 2e-05
Identities = 23/70 (32%), Positives = 42/70 (60%)
Frame = -1
Query: 590 GAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIP 411
G E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K DGM+
Sbjct: 74 GDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PVKDGMVN 130
Query: 410 YAAFLKKVMA 381
Y F++ ++A
Sbjct: 131 YHDFVQMILA 140
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 43.2 bits (97), Expect = 4e-05
Identities = 21/77 (27%), Positives = 46/77 (59%)
Frame = -1
Query: 611 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 432
+ K ++ + E++++ +++DK+ +G + A+ + LGEKL D+EV + ++ DP
Sbjct: 68 SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP- 125
Query: 431 DDDGMIPYAAFLKKVMA 381
+ G Y F++++MA
Sbjct: 126 TNSGSFDYYDFVQRIMA 142
Score = 30.7 bits (66), Expect = 0.21
Identities = 14/60 (23%), Positives = 34/60 (56%)
Frame = -1
Query: 581 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 402
++ E LYD +++GL+ + + L +LG + D+E+A+++ + D D+ + + +
Sbjct: 9 DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 29.9 bits (64), Expect = 0.38
Identities = 16/61 (26%), Positives = 31/61 (50%)
Frame = -1
Query: 629 LPXYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTK 450
L Y+ + + + +D E KL+D +++ + EL + ALG + SEV ++ +
Sbjct: 21 LGGYAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILR 80
Query: 449 D 447
D
Sbjct: 81 D 81
Score = 27.1 bits (57), Expect = 2.6
Identities = 18/75 (24%), Positives = 33/75 (44%)
Frame = -1
Query: 608 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 429
+K ++ E+ +L+D +E G + L L E +DD E+ + ++ D
Sbjct: 101 EKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DLD 158
Query: 428 DDGMIPYAAFLKKVM 384
DG I F+ +M
Sbjct: 159 QDGEINEQEFIAIMM 173
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 1.5
Identities = 13/31 (41%), Positives = 15/31 (48%)
Frame = +1
Query: 232 APPEELSPPRALPAPVPQSRASVF*GPSHRT 324
APP PP A P P+P S A P R+
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750
>SPAC22F8.07c |rtf1||replication termination factor
Rtf1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 466
Score = 26.2 bits (55), Expect = 4.6
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = -2
Query: 79 CKTHVVRVFKLLFIYLLSTF 20
CK+H R+ K LFI LSTF
Sbjct: 410 CKSHFERIKKTLFIDGLSTF 429
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 6.1
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = -1
Query: 494 LGEKLDDSEVAEVTKDCMDPED 429
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,436,328
Number of Sequences: 5004
Number of extensions: 42940
Number of successful extensions: 115
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 107
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 115
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 331187010
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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