Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P10_pT_M09
(780 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr... 174 1e-44
SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochr... 30 0.43
SPAC25B8.14 |mal2||kinetochore protein Mal2 |Schizosaccharomyces... 29 0.75
SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch... 28 1.3
SPAC16E8.04c |||chorismate mutase |Schizosaccharomyces pombe|chr... 27 2.3
SPBC651.03c |gyp10||GTPase activating protein Gyp10|Schizosaccha... 27 4.0
SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po... 27 4.0
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 26 5.3
SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor Cwf22|Schizosacch... 26 7.0
SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomy... 25 9.2
SPAC17C9.10 |stm1||G-protein coupled receptor Stm1|Schizosacchar... 25 9.2
SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ... 25 9.2
>SPAC3F10.03 |||glycine tRNA-ligase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 652
Score = 174 bits (424), Expect = 1e-44
Identities = 85/219 (38%), Positives = 135/219 (61%)
Frame = -3
Query: 778 DSQAINDTLXTLDNAALEEMQKELDSNGEYTLITXRGEFKLTPSLVNVKKTQKTIHVEEI 599
D++A+ + + + + E +L + G+ + E + LV ++K + T H+
Sbjct: 434 DAKAVEEAMISWPESEKVEKSAQLVAEGKIIVNVNGVEHTVESDLVTIEKRKHTEHIRTY 493
Query: 598 IPSVIEPSFGVGRILYCILEHNFRMREGDEQRTYFSLPPTVAPMKCVVLPLSGNAEFQPF 419
P+VIEPSFG+GRILY ++EH + R D R S P ++AP+K +++PLS NAEF PF
Sbjct: 494 TPNVIEPSFGLGRILYVLMEHAYWTRPEDVNRGVLSFPASIAPIKALIVPLSRNAEFAPF 553
Query: 418 VRDLSQELITVDVSHKVDDSSGSIGRRYARTDELGVPYAVTVDFDTIKEPHTVTLRERDS 239
V+ LS +L + +S+K+DDS+ +IGRRYAR DELG P+ +TVDF+T++ T+TLRERDS
Sbjct: 554 VKKLSAKLRNLGISNKIDDSNANIGRRYARNDELGTPFGLTVDFETLQN-ETITLRERDS 612
Query: 238 MRQVRLPMADVPTVVRDLSNSKILWSDVEQKYPKFEQQE 122
+QVR +V + + K + D K+ +F+ +
Sbjct: 613 TKQVRGSQDEVIAALVSMVEGKSSFEDALAKFGEFKSTQ 651
>SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated
heterochromatin assembly Hrr1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1015
Score = 29.9 bits (64), Expect = 0.43
Identities = 30/91 (32%), Positives = 45/91 (49%), Gaps = 8/91 (8%)
Frame = -3
Query: 745 LDNAALEEMQKELDSNGEYTLITXRGEF-KLTPSLVNVKKTQKTIHVEEI-----IPSVI 584
+D LEE+QKE + +G RG+F L + K T T+H +EI IP++
Sbjct: 571 IDPEKLEEIQKEAEDSGALMEEELRGKFINLRCKYLFSKLT--TLHEKEIDTLLTIPNIW 628
Query: 583 E-PSFGVGRILYC-ILEHNFRMREGDEQRTY 497
+ P + G I+YC LE + E + R Y
Sbjct: 629 DIPEYSRG-IIYCRWLESAYAAAEKELNRLY 658
>SPAC25B8.14 |mal2||kinetochore protein Mal2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 303
Score = 29.1 bits (62), Expect = 0.75
Identities = 13/42 (30%), Positives = 24/42 (57%)
Frame = +3
Query: 171 ILLLDKSLTTVGTSAMGNLTCLILSLSLRVTVWGSLIVSKST 296
+L ++KSLT GT+ NL+C L + + + +L+ S +
Sbjct: 213 LLQINKSLTIDGTNISANLSCTHLVIQIPKVIQATLVCSSES 254
>SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 425
Score = 28.3 bits (60), Expect = 1.3
Identities = 16/66 (24%), Positives = 31/66 (46%), Gaps = 1/66 (1%)
Frame = -3
Query: 487 PPTVAPMKCVVLPLSGN-AEFQPFVRDLSQELITVDVSHKVDDSSGSIGRRYARTDELGV 311
P ++AP K +V+P S N + V D + ++ D D + + G + + +G
Sbjct: 323 PSSIAPWKVLVVPTSDNHIQSAETVYDATANVVGFDNVLLEDRQNRAFGYKMRDAELIGY 382
Query: 310 PYAVTV 293
P+ + V
Sbjct: 383 PFVIVV 388
>SPAC16E8.04c |||chorismate mutase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 251
Score = 27.5 bits (58), Expect = 2.3
Identities = 14/43 (32%), Positives = 21/43 (48%)
Frame = -3
Query: 706 DSNGEYTLITXRGEFKLTPSLVNVKKTQKTIHVEEIIPSVIEP 578
D+ E L G+F L P+ VNV ++ EI+P + P
Sbjct: 86 DNLPEPILPKFSGKFPLHPNNVNVNSEILEYYINEIVPKISSP 128
>SPBC651.03c |gyp10||GTPase activating protein
Gyp10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 373
Score = 26.6 bits (56), Expect = 4.0
Identities = 15/33 (45%), Positives = 20/33 (60%)
Frame = +2
Query: 56 SCSISKSSCANLRQLHRCTFHSFLLFKLRIFLL 154
S S S+SS A+ Q+H + SF +KL FLL
Sbjct: 61 SRSTSQSSYADQNQVHLDSERSFFQYKLNPFLL 93
>SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 949
Score = 26.6 bits (56), Expect = 4.0
Identities = 9/27 (33%), Positives = 18/27 (66%)
Frame = -3
Query: 478 VAPMKCVVLPLSGNAEFQPFVRDLSQE 398
+ P+ +P++ EFQP++ ++SQE
Sbjct: 59 ITPVTLSPIPITPVREFQPYLHEISQE 85
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 26.2 bits (55), Expect = 5.3
Identities = 31/110 (28%), Positives = 46/110 (41%), Gaps = 6/110 (5%)
Frame = +3
Query: 24 TSSQSIQSMXHHAQSPKAAVQTCVSYTD----VPFTVSCCSNLGYFCSTSDHNILLLDKS 191
TSS + S + S AA S T T S S++ +T + S
Sbjct: 988 TSSVATASATDSSTSSIAAASVTGSSTSSVATASVTDSSTSSVATASATDSSTSSIAVAS 1047
Query: 192 LTTVGTSAMGNLTCLILSLSLRVT--VWGSLIVSKSTVTA*GTPSSSVLA 335
+T TS++ + S S T + GSL S +T + G+P+SSV A
Sbjct: 1048 VTGSSTSSVATASATDSSTSSVATASITGSLSSSIATASVTGSPTSSVTA 1097
>SPBC13E7.01 |cwf22|SPBC15D4.16|splicing factor
Cwf22|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 25.8 bits (54), Expect = 7.0
Identities = 21/65 (32%), Positives = 33/65 (50%), Gaps = 2/65 (3%)
Frame = -3
Query: 736 AALEEMQKEL-DSNG-EYTLITXRGEFKLTPSLVNVKKTQKTIHVEEIIPSVIEPSFGVG 563
A L+ +Q +L D N EY + K L+N K K+ ++ +IIP + + + G
Sbjct: 98 AKLKALQAQLTDVNTPEYQRMQWEALKKSINGLIN--KVNKS-NIRDIIPELFQENIIRG 154
Query: 562 RILYC 548
R LYC
Sbjct: 155 RALYC 159
>SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 603
Score = 25.4 bits (53), Expect = 9.2
Identities = 12/36 (33%), Positives = 19/36 (52%)
Frame = -3
Query: 226 RLPMADVPTVVRDLSNSKILWSDVEQKYPKFEQQET 119
+LP A PT+ RD S + ++ + Y E +ET
Sbjct: 390 QLPSASPPTMFRDHSFAPLVSPSIVSPYGVLENEET 425
>SPAC17C9.10 |stm1||G-protein coupled receptor
Stm1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 271
Score = 25.4 bits (53), Expect = 9.2
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = +3
Query: 111 PFTVSCCSNLGYFCSTSDHNILLLDKSLTTVGTS 212
PFT C S++ YFC+ I+ K+ +T G S
Sbjct: 178 PFTAGCISSVLYFCARIP-QIIKNHKAKSTEGLS 210
>SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2111
Score = 25.4 bits (53), Expect = 9.2
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +3
Query: 555 KILPTPNEGSMTLGIISS 608
++ P P EG+ TLGII S
Sbjct: 1707 RVCPAPCEGACTLGIIES 1724
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,242,939
Number of Sequences: 5004
Number of extensions: 67911
Number of successful extensions: 192
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 191
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 377352472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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