Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P10_pT_I07
(690 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 146 3e-36
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 67 2e-12
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 64 2e-11
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 47 2e-06
SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc... 27 1.9
SPCPB16A4.04c |trm8||tRNA |Schizosaccharomyces pombe|chr 3|||Manual 27 2.6
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 27 3.4
SPBC13E7.06 |msd1|mug172|spindle pole body protein Msd1|Schizosa... 26 5.9
SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 25 7.8
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 146 bits (353), Expect = 3e-36
Identities = 64/83 (77%), Positives = 74/83 (89%)
Frame = -3
Query: 688 VCDIPPKGLKMSXTFIGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFNEAE 509
VC +PPK LKMS TFIGN+T+IQE+F+R+ +QFSAMFRRKAFLHWYTGEGMDEMEF EAE
Sbjct: 353 VCSVPPKDLKMSATFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAE 412
Query: 508 SNVNDLVSEYQQYQEATAEDDTE 440
SN+NDLVSEYQQYQEA ++ E
Sbjct: 413 SNMNDLVSEYQQYQEAGIDEGDE 435
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 67.3 bits (157), Expect = 2e-12
Identities = 31/89 (34%), Positives = 53/89 (59%), Gaps = 8/89 (8%)
Frame = -3
Query: 688 VCDIPPKGLKMSXT--------FIGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMD 533
+CD PP+ ++ S + NTT+I E + R+ +F M+ ++AF+HWY GEGM+
Sbjct: 355 ICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGME 414
Query: 532 EMEFNEAESNVNDLVSEYQQYQEATAEDD 446
E EF+EA ++ L +Y++ + + E D
Sbjct: 415 EGEFSEAREDLAALERDYEEVGQDSMEVD 443
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 64.1 bits (149), Expect = 2e-11
Identities = 24/66 (36%), Positives = 45/66 (68%)
Frame = -3
Query: 643 IGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFNEAESNVNDLVSEYQQYQE 464
+ NTT+I E + R+ +F M+ ++AF+HWY GEGM+E EF+EA ++ L +Y++ +
Sbjct: 382 LSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALERDYEEVGQ 441
Query: 463 ATAEDD 446
+ +++
Sbjct: 442 DSMDNE 447
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 47.2 bits (107), Expect = 2e-06
Identities = 24/69 (34%), Positives = 41/69 (59%), Gaps = 3/69 (4%)
Frame = -3
Query: 661 KMSXTFIGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFNEAESN---VNDL 491
++S + N T+I LFKR +Q+ + +R AFL Y E + E + NE +S+ V DL
Sbjct: 372 RVSGLMLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVVADL 431
Query: 490 VSEYQQYQE 464
++EY+ ++
Sbjct: 432 INEYEACED 440
>SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase
Shk1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 658
Score = 27.5 bits (58), Expect = 1.9
Identities = 24/79 (30%), Positives = 32/79 (40%), Gaps = 3/79 (3%)
Frame = +1
Query: 196 IESFEMVVSPPGTLGLVSARDLASNYTREG*I*QHPRDS---PYYAASMRFRFESELQRS 366
I E V P L ++ + Y EG PR+S P + S S LQ +
Sbjct: 184 ITKSEQVQHPQAVLDAMAFYSQSKKYLEEGAKPPFPRESTEKPLLSVSA-LSSSSHLQPT 242
Query: 367 **GSEVKSSHGARPAPTPP 423
S + +RPAPTPP
Sbjct: 243 SATSSSSRLYPSRPAPTPP 261
>SPCPB16A4.04c |trm8||tRNA |Schizosaccharomyces pombe|chr 3|||Manual
Length = 273
Score = 27.1 bits (57), Expect = 2.6
Identities = 14/37 (37%), Positives = 19/37 (51%)
Frame = -2
Query: 254 RADTRPSVPGGLTTISKLSMRXRTTTRHAFDEAQSAK 144
RAD VPGG IS L M + + F++ Q +K
Sbjct: 130 RADHEEPVPGGYKNISVLRMNCQKFLPNFFEKGQLSK 166
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 26.6 bits (56), Expect = 3.4
Identities = 19/59 (32%), Positives = 29/59 (49%)
Frame = -3
Query: 643 IGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFNEAESNVNDLVSEYQQYQ 467
+ N + L+K + E+FS +F RK L WY G+ E + N+N SE + Q
Sbjct: 1703 LNNPHLLFTLYKLL-ERFSLIFLRKCALLWYCRYGVS----FETQPNLNFQNSELSRLQ 1756
>SPBC13E7.06 |msd1|mug172|spindle pole body protein
Msd1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 331
Score = 25.8 bits (54), Expect = 5.9
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = +3
Query: 498 LTLLSASLNSISSMPSPVYQ 557
LT LS +LNS+ M SP+Y+
Sbjct: 234 LTCLSGTLNSLVEMLSPLYE 253
>SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase
Ppk29|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 25.4 bits (53), Expect = 7.8
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = -3
Query: 637 NTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDE 530
N++ IQ L K I+ + +R ++ Y+G G+DE
Sbjct: 193 NSSEIQALEKSINTFTTYQYRAPEMINLYSGLGIDE 228
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,204,398
Number of Sequences: 5004
Number of extensions: 35293
Number of successful extensions: 81
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 78
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 80
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 319939482
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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