Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P10_pT_I02
(784 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 184 1e-47
SPAC2F7.05c |||translation initiation factor eIF5 |Schizosacchar... 49 9e-07
SPBC1347.07 |rex2||RNA exonuclease|Schizosaccharomyces pombe|chr... 28 1.3
SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 27 3.0
SPBC646.15c |||Pex16 family protein|Schizosaccharomyces pombe|ch... 27 4.0
SPBC16G5.01 |rpn12|mts3, SPBC342.07|19S proteasome regulatory su... 26 5.3
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 26 7.0
SPAC9E9.12c |ybt1|abc1|ABC transporter Ybt1|Schizosaccharomyces ... 25 9.3
SPAC16E8.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 25 9.3
>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 321
Score = 184 bits (448), Expect = 1e-47
Identities = 88/142 (61%), Positives = 110/142 (77%)
Frame = -3
Query: 779 RVFDIMREKNPSMVSGKKQKFIMRPPQVVRIGTKKTSFANFTEICKTLHRQAKHLLDFLL 600
R F ++R NP + +G+K+K+ + PP V R G KKT FAN ++I K +HR H++ FL
Sbjct: 176 RFFTLLRTNNPEL-AGEKRKYTIVPPSVHREG-KKTIFANISDISKRMHRSLDHVIQFLF 233
Query: 599 AELGTSGSVDGNSQLIIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLFFLQ 420
AELGTSGSVDG+S+LIIKGRFQQKQIENVLRRYI EYVTC TC+SPDTIL K+ R+FF+
Sbjct: 234 AELGTSGSVDGSSRLIIKGRFQQKQIENVLRRYIVEYVTCKTCKSPDTILTKENRIFFMT 293
Query: 419 CETCGSRCSVASIKSGFQAVTG 354
CE CGS SV +IK+G+QA G
Sbjct: 294 CEACGSVRSVQAIKTGYQAQIG 315
>SPAC2F7.05c |||translation initiation factor eIF5
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 395
Score = 48.8 bits (111), Expect = 9e-07
Identities = 26/96 (27%), Positives = 49/96 (51%), Gaps = 3/96 (3%)
Frame = -3
Query: 677 KTSFANFTEICKTLHRQAKHLLDFLLAELGTSGSVDGN-SQLIIKGRFQQKQIENVLRRY 501
KT N ++I K L R ++ F ELG ++ + + I+ G +++++L +
Sbjct: 34 KTVIPNMSDIAKALGRPPLYVTKFFGFELGAQTTIIADMDRYIVNGAHDAGKLQDLLDVF 93
Query: 500 IKEYVTCHTCRSPDTIL--QKDTRLFFLQCETCGSR 399
I+ +V C +C++P+T L K + C+ CG R
Sbjct: 94 IRRFVLCASCQNPETELSINKKDQTISYDCKACGYR 129
>SPBC1347.07 |rex2||RNA exonuclease|Schizosaccharomyces pombe|chr
2|||Manual
Length = 180
Score = 28.3 bits (60), Expect = 1.3
Identities = 18/77 (23%), Positives = 32/77 (41%)
Frame = -3
Query: 716 IMRPPQVVRIGTKKTSFANFTEICKTLHRQAKHLLDFLLAELGTSGSVDGNSQLIIKGRF 537
+M ++ G + F + K +Q + D+ + + G SG + Q
Sbjct: 25 LMEVAAIITDGNLRPVEEKFDAVIKLDEKQLSEMNDWCIEQHGKSGLTERCRQ----SNL 80
Query: 536 QQKQIENVLRRYIKEYV 486
K +EN L YIK+Y+
Sbjct: 81 TVKDVENQLLAYIKKYI 97
>SPBC211.03c |||guanyl-nucleotide exchange
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1462
Score = 27.1 bits (57), Expect = 3.0
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +1
Query: 379 LMLATEQRDPHVSH*RKNNLVSFCKIVSG 465
+ML T+Q +P++ R+ L FC+ V G
Sbjct: 680 IMLNTDQHNPNIKSQRRMTLDDFCRNVRG 708
>SPBC646.15c |||Pex16 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 376
Score = 26.6 bits (56), Expect = 4.0
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = -1
Query: 682 QRKPPLPILRKFVKLCIGRPSICWIFFWRNW 590
+R P L I F+K+C RP I +F W +W
Sbjct: 211 KRLPNLRIFSNFIKVC--RPLIYMLFMW-HW 238
>SPBC16G5.01 |rpn12|mts3, SPBC342.07|19S proteasome regulatory
subunit Rpn12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 270
Score = 26.2 bits (55), Expect = 5.3
Identities = 10/21 (47%), Positives = 17/21 (80%)
Frame = +1
Query: 625 ACRCKVLQISVKLAKEVFFVP 687
AC+ ++L++ V+LAK+ FVP
Sbjct: 20 ACKKELLKLKVELAKQNLFVP 40
>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1428
Score = 25.8 bits (54), Expect = 7.0
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +2
Query: 89 VLSTPGLLYYVLLKLRTT 142
VL TPGLL Y++ K R T
Sbjct: 342 VLKTPGLLAYLITKYRCT 359
>SPAC9E9.12c |ybt1|abc1|ABC transporter Ybt1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1427
Score = 25.4 bits (53), Expect = 9.3
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = +3
Query: 453 NCIRGAACMTCYILFYIPT*NILYLFLLEATF 548
N R A C +LFY+ ++ LFL++ F
Sbjct: 15 NFFRNATLDQCLLLFYLSLFSLTNLFLIQKLF 46
>SPAC16E8.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 269
Score = 25.4 bits (53), Expect = 9.3
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = +1
Query: 577 EPLVPNSARRKSNKCLACRCKVLQISVKLAKEVFF 681
EPL N + NK L C+ K + KLA ++ +
Sbjct: 22 EPLEANLFGKLHNKLLICKAKKENLEKKLAYQMMY 56
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,475,189
Number of Sequences: 5004
Number of extensions: 74782
Number of successful extensions: 185
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 180
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 184
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 379359666
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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