Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P10_F_O04
         (756 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch...    71   2e-13
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb...    71   2e-13
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces...    54   2e-08
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma...    31   0.18 
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    28   1.7  
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo...    27   2.2  
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy...    26   6.7  

>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 150

 Score = 70.9 bits (166), Expect = 2e-13
 Identities = 47/151 (31%), Positives = 76/151 (50%), Gaps = 3/151 (1%)
 Frame = +2

Query: 65  MSDLSXXDVERASF--AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXX 238
           M+  +  D + A F  AFS++D +  G I +  LG ++R+L  +PT A +          
Sbjct: 1   MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDAD 60

Query: 239 XXXXXXXX-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKL 415
                    FL + ++  KD D    E+  E  K++DK+ NG +   ELTH L +LGE+L
Sbjct: 61  GNGTIDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERL 118

Query: 416 DDSEVAEVTKDCMDPEDDDGMIPYAAFLKKV 508
              EVA++ ++     D DG+I Y  F + +
Sbjct: 119 SQEEVADMIREA--DTDGDGVINYEEFSRVI 147


>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 141

 Score = 70.9 bits (166), Expect = 2e-13
 Identities = 44/136 (32%), Positives = 71/136 (52%)
 Frame = +2

Query: 107 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 286
           AFS++D  G G+I   ++GDLLRA   NPTLA I                  FL + ++ 
Sbjct: 11  AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67

Query: 287 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 466
                 G  E+F++  +++DK+  G++   EL + L +LGEKL + E+ E+ K       
Sbjct: 68  NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124

Query: 467 DDGMIPYAAFLKKVMA 514
            DGM+ Y  F++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140


>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 143

 Score = 54.4 bits (125), Expect = 2e-08
 Identities = 35/136 (25%), Positives = 68/136 (50%)
 Frame = +2

Query: 107 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 286
           AF +YD +  G I   ++G +LR+L  N T A +                  F+     +
Sbjct: 14  AFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFV-----S 68

Query: 287 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 466
            K ++  + E++++  +++DK+ +G +  A+    +  LGEKL D+EV  + ++  DP  
Sbjct: 69  NKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP-T 126

Query: 467 DDGMIPYAAFLKKVMA 514
           + G   Y  F++++MA
Sbjct: 127 NSGSFDYYDFVQRIMA 142



 Score = 30.7 bits (66), Expect = 0.23
 Identities = 14/60 (23%), Positives = 34/60 (56%)
 Frame = +2

Query: 314 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 493
           ++  E   LYD +++GL+  + +   L +LG  + D+E+A+++ +  D  D+   + + +
Sbjct: 9   DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68


>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 176

 Score = 31.1 bits (67), Expect = 0.18
 Identities = 29/136 (21%), Positives = 53/136 (38%), Gaps = 1/136 (0%)
 Frame = +2

Query: 107 AFSIYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXX-FLPIYSQ 283
           AF ++D +    ID   L   +RAL  N   + +                   F+ + ++
Sbjct: 42  AFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRVMTE 101

Query: 284 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 463
              ++D    E+     +L+D +E G +    L      L E +DD E+  + ++     
Sbjct: 102 KIVERDP--LEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DL 157

Query: 464 DDDGMIPYAAFLKKVM 511
           D DG I    F+  +M
Sbjct: 158 DQDGEINEQEFIAIMM 173



 Score = 29.5 bits (63), Expect = 0.54
 Identities = 15/58 (25%), Positives = 30/58 (51%)
 Frame = +2

Query: 275 YSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKD 448
           Y+  + +  +   +D  E  KL+D +++  +   EL   + ALG   + SEV ++ +D
Sbjct: 24  YAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81



 Score = 25.4 bits (53), Expect = 8.8
 Identities = 12/34 (35%), Positives = 18/34 (52%)
 Frame = +2

Query: 89  VERASFAFSIYDFEGKGKIDAFNLGDLLRALNSN 190
           +E    AF ++D +  GKI   NL  + + LN N
Sbjct: 109 LEEIKRAFELFDDDETGKISLRNLRRVAKELNEN 142


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 13/31 (41%), Positives = 15/31 (48%)
 Frame = -1

Query: 663  APPEELSPPRALPAPVPQSRASVF*GPSHRT 571
            APP    PP A P P+P S A     P  R+
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750


>SPBC215.01 ||SPBC3B9.20|GTPase activating
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 13/29 (44%), Positives = 18/29 (62%), Gaps = 1/29 (3%)
 Frame = +2

Query: 65  MSDLSXXDVER-ASFAFSIYDFEGKGKID 148
           +++L   DV R  SF F +YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633


>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1142

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 9/22 (40%), Positives = 14/22 (63%)
 Frame = +2

Query: 401 LGEKLDDSEVAEVTKDCMDPED 466
           L EK+ D +   +  DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,613,149
Number of Sequences: 5004
Number of extensions: 44021
Number of successful extensions: 125
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 121
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)