Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P10_F_L21
(790 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC25H2.03 |||vacuolar protein involved in phosphoinositide met... 108 8e-25
SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces pomb... 34 0.027
SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein Sap155|Sc... 30 0.43
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 28 1.8
SPAP8A3.09c |paa1||protein phosphatase regulatory subunit Paa1|S... 28 1.8
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 27 4.0
SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ... 27 4.0
SPAPB8E5.07c |||ribosome biogenesis protein Rrp12|Schizosaccharo... 26 7.1
SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizo... 26 7.1
SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid alpha... 26 7.1
SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|c... 25 9.4
SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces pomb... 25 9.4
>SPBC25H2.03 |||vacuolar protein involved in phosphoinositide
metabolism|Schizosaccharomyces pombe|chr 2|||Manual
Length = 811
Score = 108 bits (260), Expect = 8e-25
Identities = 54/132 (40%), Positives = 82/132 (62%), Gaps = 2/132 (1%)
Frame = +2
Query: 221 MVKDFNDAKNTSQIKKLIKVLGQDLMSST--NPNMKNGALMGLSTVAVGLGKASVDYLPE 394
+VK + + T +I+ +I L D + S PN G L+GL+ VA+ LG Y+
Sbjct: 27 VVKGYLENDETEKIRAVISQLANDFVYSPARGPNATFGGLIGLAAVAIALGPKIDSYMES 86
Query: 395 LTNPIVACFSESESRVRYQAAEALFNVLKIVRGASLSQFPVIFDALAKLAADPEQQVKHA 574
+ P++ CF++S+S++RY A E+++N+ K+ +G F +IFD L KL AD E VK+
Sbjct: 87 ILLPVLYCFNDSDSKIRYYACESMYNIGKVAKGEVFRYFNLIFDVLCKLFADTEITVKNG 146
Query: 575 AELLDRLLKDIV 610
AELLDRL+KDIV
Sbjct: 147 AELLDRLIKDIV 158
Score = 48.0 bits (109), Expect = 2e-06
Identities = 20/44 (45%), Positives = 29/44 (65%)
Frame = +2
Query: 659 ERMYTRSAPARQLAVSWVAVLDAVPDLNVLAHLPDLLDGLFXML 790
ER+Y + R VSW+ +LD++PDL +++LP LLDGL L
Sbjct: 211 ERLYVINPNTRMFLVSWIRLLDSIPDLEFISYLPFLLDGLMNYL 254
>SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1095
Score = 33.9 bits (74), Expect = 0.027
Identities = 25/97 (25%), Positives = 47/97 (48%), Gaps = 2/97 (2%)
Frame = +2
Query: 293 LMSSTNPNMKNGALMGLSTVAVGLGKASVDYLPELTNPIVACFSESESRVRYQAAEALFN 472
L+ S + ++ ALM +S++A G K L + + ++ ++ RVR+ A A+
Sbjct: 379 LIPSQKWSERHAALMAISSIAEGAEKLMKKELSRVLDMVLPLLADPHPRVRWAACNAVGQ 438
Query: 473 VLKIVRGASLSQFPV-IFDALAKLAADPEQQVK-HAA 577
+ ++P I +AL + PE +V+ HAA
Sbjct: 439 MSTDFAPDMQVKYPSRILEALVPVLESPESRVQAHAA 475
>SPAC27F1.09c |prp10|sap155|U2 snRNP-associated protein
Sap155|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1188
Score = 29.9 bits (64), Expect = 0.43
Identities = 14/49 (28%), Positives = 27/49 (55%)
Frame = +2
Query: 344 STVAVGLGKASVDYLPELTNPIVACFSESESRVRYQAAEALFNVLKIVR 490
STV LG YLP++ + I+ + + VR QAA+ + ++ +++
Sbjct: 799 STVVNALGTRCKPYLPQIVSTILYRLNNKSANVREQAADLVSSITIVLK 847
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 27.9 bits (59), Expect = 1.8
Identities = 18/44 (40%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
Frame = +2
Query: 269 LIKVLGQDLMSSTNPNMKNGALMGLSTVAVGLGKASV-DYLPEL 397
LI L L S + + GA GLS + GLG A + D LPE+
Sbjct: 1680 LIPELFNVLRSECSEVDRQGAAQGLSEILAGLGLARLEDVLPEI 1723
>SPAP8A3.09c |paa1||protein phosphatase regulatory subunit
Paa1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 590
Score = 27.9 bits (59), Expect = 1.8
Identities = 20/60 (33%), Positives = 29/60 (48%)
Frame = +2
Query: 395 LTNPIVACFSESESRVRYQAAEALFNVLKIVRGASLSQFPVIFDALAKLAADPEQQVKHA 574
L P+ + S+S R RY A + K+V G SL + +I L D EQ+V+ A
Sbjct: 246 LLQPLRSFVSDSSWRTRYMVAANFVKLAKVV-GPSLIKDELI-KPFVLLMKDTEQEVRRA 303
>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 874
Score = 26.6 bits (56), Expect = 4.0
Identities = 24/82 (29%), Positives = 34/82 (41%), Gaps = 10/82 (12%)
Frame = +2
Query: 209 TTKPMVKDFNDAKNTSQIKKLIK--------VLGQDLMSSTNPNMKNGALMGLSTVAVGL 364
T +V D N K SQ + I V +L+ S + N L V +
Sbjct: 703 TKLTIVDDHNSRKLESQYNEFISQCSAHYENVFSLNLVISDAMKLTNNIADALKNNKVNI 762
Query: 365 G--KASVDYLPELTNPIVACFS 424
G KAS++ L + PI+ CFS
Sbjct: 763 GTIKASLEVLVKCIAPIIPCFS 784
>SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 937
Score = 26.6 bits (56), Expect = 4.0
Identities = 20/69 (28%), Positives = 28/69 (40%), Gaps = 3/69 (4%)
Frame = +2
Query: 398 TNPIVACFSESESRVRYQAAEALFNVLKIVRGASLSQFPVIFDALAKLAAD---PEQQVK 568
TN + A Q+ AL+ L +V+G PV+F L KL D PE+ +
Sbjct: 446 TNLLAAAIDTRMFHENTQSDAALYKRLTLVKGNKREFAPVMFRRLKKLGIDKTNPEELTE 505
Query: 569 HAAELLDRL 595
RL
Sbjct: 506 EEQRKFARL 514
>SPAPB8E5.07c |||ribosome biogenesis protein
Rrp12|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1163
Score = 25.8 bits (54), Expect = 7.1
Identities = 17/84 (20%), Positives = 34/84 (40%)
Frame = +2
Query: 230 DFNDAKNTSQIKKLIKVLGQDLMSSTNPNMKNGALMGLSTVAVGLGKASVDYLPELTNPI 409
D+ A T +K++ + L + + + L L+ + V +P+L
Sbjct: 724 DYGKAYATQHVKEIFEELSS-VADRVVSSTRKDRLASLNALYELQSSELVIAIPQLLPEA 782
Query: 410 VACFSESESRVRYQAAEALFNVLK 481
+ E + R+ A + LFN+ K
Sbjct: 783 IISLKEVNEKARHTAFQLLFNIAK 806
>SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1055
Score = 25.8 bits (54), Expect = 7.1
Identities = 23/53 (43%), Positives = 28/53 (52%)
Frame = +2
Query: 425 ESESRVRYQAAEALFNVLKIVRGASLSQFPVIFDALAKLAADPEQQVKHAAEL 583
E E R+R + EA N + V S S+F AL KLA DPE K A+EL
Sbjct: 186 EFEERLRRKDLEAATN--EFVEDYS-SKFSSEELALRKLADDPESWRKLASEL 235
>SPAC56F8.06c |alg10||dolichyl-phosphate-glucose-glycolipid
alpha-glucosyltransferase Alg10|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 445
Score = 25.8 bits (54), Expect = 7.1
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -2
Query: 273 INFFIWLVFFASLKSFTI 220
IN+F+W FF S S+ I
Sbjct: 259 INYFLWFFFFFSFPSYII 276
>SPBC16E9.14c |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 386
Score = 25.4 bits (53), Expect = 9.4
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = -2
Query: 369 LPSPTATVDRPISAPFFILGLVEDIKSWPKTLINFFIW 256
L S AT+ +S + G++ I W +TL +F +W
Sbjct: 112 LLSGDATILLTLSCSLIVEGVLIIINVWRETLDSFLVW 149
>SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 923
Score = 25.4 bits (53), Expect = 9.4
Identities = 16/52 (30%), Positives = 24/52 (46%)
Frame = -2
Query: 465 NASAAWYLTRDSLSLKQATIGFVNSGK*STDALPSPTATVDRPISAPFFILG 310
+ S WY +L L F+ G ++D S +A V RP+ P F +G
Sbjct: 332 STSTHWYSESGTLDL------FIFLGPKASDVYESYSALVGRPLLPPLFSIG 377
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,815,525
Number of Sequences: 5004
Number of extensions: 53420
Number of successful extensions: 156
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 156
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 383374054
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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