Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P10_F_G08
(853 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 113 3e-26
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 79 8e-16
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 77 2e-15
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 49 7e-07
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 46 5e-06
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 44 2e-05
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 40 4e-04
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 40 6e-04
SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|ch... 32 0.12
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 29 0.63
SPAC4H3.12c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 2.6
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 113 bits (272), Expect = 3e-26
Identities = 70/217 (32%), Positives = 101/217 (46%), Gaps = 7/217 (3%)
Frame = +1
Query: 220 SSDVIELTPSN-FDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALK--GIVKVGA 390
+S V+EL N + + S + +IEF+A WCGHCKSL P Y++ + V +G
Sbjct: 19 ASGVVELQSLNELENTIRASKKGALIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGK 78
Query: 391 LDADEHRSVSQKYGVTGFPTIKIF--TGSKHTPYQGQRTAEGFVXXXXXXXXXXXXXNLX 564
+DAD H V+ KY +TGFPT+ F GS+ Y R +
Sbjct: 79 IDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSLT---------QFVSEKT 129
Query: 565 XXXXXXXXXXXXVITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKG 744
V+ L NF ++V+D LVEFYA WCG+CK L P + K
Sbjct: 130 GIKKRKIVLPSNVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLGKVFKN 189
Query: 745 K--VKLGALXATVHTTMASRYQVQGYPTIKLFPSGXK 849
+ V++ + A V + ++V +PTIK FP K
Sbjct: 190 EPNVEIVKINADVFADIGRLHEVASFPTIKFFPKDDK 226
Score = 73.7 bits (173), Expect = 3e-14
Identities = 37/103 (35%), Positives = 58/103 (56%), Gaps = 5/103 (4%)
Frame = +1
Query: 223 SDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKG--IVKVGALD 396
S+V+EL NFDK+V + + ++EF+A WCG+CK L P Y+ + K V++ ++
Sbjct: 140 SNVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLGKVFKNEPNVEIVKIN 199
Query: 397 ADEHRSVSQKYGVTGFPTIKIF-TGSKHTP--YQGQRTAEGFV 516
AD + + + V FPTIK F K P Y+G R+ E +
Sbjct: 200 ADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLI 242
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 79.0 bits (186), Expect = 8e-16
Identities = 44/101 (43%), Positives = 60/101 (59%), Gaps = 8/101 (7%)
Frame = +1
Query: 223 SDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGAL--D 396
S+ IEL NF K V ++ F+APWCG+CK LVP Y+K A L ++ V A+ D
Sbjct: 31 SNTIELNSKNFRKFVKAKGPSLVV-FYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCD 89
Query: 397 ADEHRSVSQKYGVTGFPTIK-IFTGSK-----HTPYQGQRT 501
AD++R+V +Y V GFPTIK ++ SK T Y G R+
Sbjct: 90 ADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRS 130
Score = 65.7 bits (153), Expect = 8e-12
Identities = 37/86 (43%), Positives = 50/86 (58%), Gaps = 3/86 (3%)
Frame = +1
Query: 604 ITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKLGAL--XATV 777
I L NF++ V LV FYAPWCG+CK L P + K A+ L + + A+ A
Sbjct: 34 IELNSKNFRKFVKAKGPS-LVVFYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQ 92
Query: 778 HTTMASRYQVQGYPTIKL-FPSGXKS 852
+ + S+YQVQG+PTIKL +PS S
Sbjct: 93 NRAVCSQYQVQGFPTIKLVYPSSKGS 118
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 77.4 bits (182), Expect = 2e-15
Identities = 34/102 (33%), Positives = 63/102 (61%), Gaps = 2/102 (1%)
Frame = +1
Query: 217 SSSDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARAL-KGIVKVGAL 393
+S++V ++ ++L+T +D++ +++F+APWCGHCK+L PEY+ AA L K + + +
Sbjct: 20 ASAEVPKVNKEGLNELIT-ADKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEV 78
Query: 394 DADEHRSVSQKYGVTGFPTIKIF-TGSKHTPYQGQRTAEGFV 516
D E + +Y + G+PT+ +F G + + Y G R + V
Sbjct: 79 DCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALV 120
Score = 74.9 bits (176), Expect = 1e-14
Identities = 41/103 (39%), Positives = 58/103 (56%), Gaps = 5/103 (4%)
Frame = +1
Query: 214 DSSSDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALK--GIVKVG 387
+S D++ L NFD +V + + ++EF+APWCGHCK+L P Y+K A V V
Sbjct: 352 ESQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVA 411
Query: 388 ALDADEHRSVSQKYGVTGFPTIKIF-TGSKHTP--YQGQRTAE 507
+DA E+ +S ++GFPTI F K P Y+G RT E
Sbjct: 412 KIDATEN-DIS--VSISGFPTIMFFKANDKVNPVRYEGDRTLE 451
Score = 69.3 bits (162), Expect = 6e-13
Identities = 29/70 (41%), Positives = 44/70 (62%), Gaps = 1/70 (1%)
Frame = +1
Query: 637 VLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATEL-KGKVKLGALXATVHTTMASRYQVQG 813
++ +D + +V+FYAPWCGHCK L P + AA EL K + L + T + S Y ++G
Sbjct: 35 LITADKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRG 94
Query: 814 YPTIKLFPSG 843
YPT+ +F +G
Sbjct: 95 YPTLNVFKNG 104
Score = 66.9 bits (156), Expect = 3e-12
Identities = 34/85 (40%), Positives = 46/85 (54%), Gaps = 2/85 (2%)
Frame = +1
Query: 601 VITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELK--GKVKLGALXAT 774
++ L NF ++V+D LVEFYAPWCGHCKNL P + K A E V + + AT
Sbjct: 357 LVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDAT 416
Query: 775 VHTTMASRYQVQGYPTIKLFPSGXK 849
+ S + G+PTI F + K
Sbjct: 417 ENDISVS---ISGFPTIMFFKANDK 438
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 49.2 bits (112), Expect = 7e-07
Identities = 21/64 (32%), Positives = 40/64 (62%)
Frame = +1
Query: 271 NSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPT 450
++D++ +++F+A WCG CK L P +K + + + A++AD+ ++QK GV PT
Sbjct: 33 SADKVTVVDFYADWCGPCKYLKPFLEKLSEQNQKASFI-AVNADKFSDIAQKNGVYALPT 91
Query: 451 IKIF 462
+ +F
Sbjct: 92 MVLF 95
Score = 46.0 bits (104), Expect = 7e-06
Identities = 24/68 (35%), Positives = 36/68 (52%)
Frame = +1
Query: 640 LDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKLGALXATVHTTMASRYQVQGYP 819
+ +D + +V+FYA WCG CK L+P K +E K A+ A + +A + V P
Sbjct: 32 ISADKVTVVDFYADWCGPCKYLKPFLEK-LSEQNQKASFIAVNADKFSDIAQKNGVYALP 90
Query: 820 TIKLFPSG 843
T+ LF G
Sbjct: 91 TMVLFRKG 98
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 46.4 bits (105), Expect = 5e-06
Identities = 22/94 (23%), Positives = 49/94 (52%), Gaps = 1/94 (1%)
Frame = +1
Query: 229 VIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEH 408
V +++ S+ K + D++ +++FFA WCG CK++ P++++ + + +D D+
Sbjct: 2 VKQVSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFI-KVDVDQL 60
Query: 409 RSVSQKYGVTGFPTIKIF-TGSKHTPYQGQRTAE 507
++ + GV P+ ++ G K G A+
Sbjct: 61 SEIAAEAGVHAMPSFFLYKNGEKIEEIVGANPAK 94
Score = 36.7 bits (81), Expect = 0.004
Identities = 21/77 (27%), Positives = 37/77 (48%)
Frame = +1
Query: 619 SNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKLGALXATVHTTMASR 798
S FK +V D L +V+F+A WCG CK + P + + + + + + +A+
Sbjct: 9 SEFKSIVCQ-DKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFI-KVDVDQLSEIAAE 66
Query: 799 YQVQGYPTIKLFPSGXK 849
V P+ L+ +G K
Sbjct: 67 AGVHAMPSFFLYKNGEK 83
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 44.4 bits (100), Expect = 2e-05
Identities = 16/68 (23%), Positives = 31/68 (45%)
Frame = +1
Query: 631 ELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKLGALXATVHTTMASRYQVQ 810
+ L + W ++FY+ C C ++ W A ++GK+ + + V +Y +Q
Sbjct: 292 DAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRMRGKLNVAHINCAVSKRACKQYSIQ 351
Query: 811 GYPTIKLF 834
+PT F
Sbjct: 352 YFPTFLFF 359
Score = 40.3 bits (90), Expect = 3e-04
Identities = 22/83 (26%), Positives = 39/83 (46%), Gaps = 3/83 (3%)
Frame = +1
Query: 604 ITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKLGAL---XAT 774
+ LTD++ + V S W +++Y P CG CK L P W + K +V+
Sbjct: 29 VPLTDNDLESEV--SKGTWFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVD 86
Query: 775 VHTTMASRYQVQGYPTIKLFPSG 843
++S ++ PT+ L+ +G
Sbjct: 87 CSKELSSCANIRAVPTLYLYQNG 109
Score = 37.1 bits (82), Expect = 0.003
Identities = 18/82 (21%), Positives = 36/82 (43%)
Frame = +1
Query: 247 SNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQK 426
++ D +T+ E W I+F++ C C + + A ++G + V ++ + ++
Sbjct: 289 ADIDAALTDK-EGWFIQFYSSECDDCDDVSTAWYAMANRMRGKLNVAHINCAVSKRACKQ 347
Query: 427 YGVTGFPTIKIFTGSKHTPYQG 492
Y + FPT F Y G
Sbjct: 348 YSIQYFPTFLFFKEEAFVEYVG 369
Score = 30.3 bits (65), Expect = 0.36
Identities = 18/80 (22%), Positives = 35/80 (43%), Gaps = 3/80 (3%)
Frame = +1
Query: 232 IELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGAL---DAD 402
+ LT ++ + V+ W I+++ P CG CK L P + K V+ + D
Sbjct: 29 VPLTDNDLESEVSKGT--WFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVD 86
Query: 403 EHRSVSQKYGVTGFPTIKIF 462
+ +S + PT+ ++
Sbjct: 87 CSKELSSCANIRAVPTLYLY 106
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 39.9 bits (89), Expect = 4e-04
Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
Frame = +1
Query: 292 IEFFAPWCGHCKSLVPEYKKAARALKGIVKVGA-LDADEHRSVSQKYGVTGFPTIKIFTG 468
++ +A WCG CK++ P + + A V A ++ DE R ++ GV PT F
Sbjct: 24 VDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFEN 83
Query: 469 SK 474
K
Sbjct: 84 GK 85
Score = 35.1 bits (77), Expect = 0.013
Identities = 18/61 (29%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Frame = +1
Query: 664 VEFYAPWCGHCKNLEPHWAKAATELKG-KVKLGALXATVHTTMASRYQVQGYPTIKLFPS 840
V+ YA WCG CK + P +++ A++ K + +AS V+ PT F +
Sbjct: 24 VDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFEN 83
Query: 841 G 843
G
Sbjct: 84 G 84
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 39.5 bits (88), Expect = 6e-04
Identities = 20/83 (24%), Positives = 43/83 (51%), Gaps = 2/83 (2%)
Frame = +1
Query: 232 IELT-PSNFDKLVTNSDE-IWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADE 405
+E+T F +++ N E I ++ F+APW CK + + + A+ K V + ++A++
Sbjct: 3 VEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAVFL-KIEAEK 61
Query: 406 HRSVSQKYGVTGFPTIKIFTGSK 474
+++ + V P + G+K
Sbjct: 62 FSDIAESFDVNAVPLFVLIHGAK 84
Score = 33.1 bits (72), Expect = 0.051
Identities = 19/70 (27%), Positives = 34/70 (48%), Gaps = 1/70 (1%)
Frame = +1
Query: 625 FKELVLDS-DDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKLGALXATVHTTMASRY 801
F+E++ + + + L+ FYAPW CK + + + A + K V L + A + +A +
Sbjct: 11 FQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAVFL-KIEAEKFSDIAESF 69
Query: 802 QVQGYPTIKL 831
V P L
Sbjct: 70 DVNAVPLFVL 79
>SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 764
Score = 31.9 bits (69), Expect = 0.12
Identities = 16/37 (43%), Positives = 20/37 (54%)
Frame = +1
Query: 193 TGSLALYDSSSDVIELTPSNFDKLVTNSDEIWIIEFF 303
TGS + SDV+ L+ S L+ SD WII FF
Sbjct: 428 TGSPLAHVLCSDVVNLSVSTVFNLLCGSDTTWIINFF 464
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 29.5 bits (63), Expect = 0.63
Identities = 13/60 (21%), Positives = 24/60 (40%)
Frame = +1
Query: 313 CGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPTIKIFTGSKHTPYQG 492
C C + R +K+ ++ DE + + + + FPT ++F G Y G
Sbjct: 209 CEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNG 268
Score = 27.1 bits (57), Expect = 3.4
Identities = 9/50 (18%), Positives = 22/50 (44%)
Frame = +1
Query: 685 CGHCKNLEPHWAKAATELKGKVKLGALXATVHTTMASRYQVQGYPTIKLF 834
C C + E W+ ++K+ + M + + ++ +PT ++F
Sbjct: 209 CEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVF 258
>SPAC4H3.12c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 101
Score = 27.5 bits (58), Expect = 2.6
Identities = 8/26 (30%), Positives = 18/26 (69%)
Frame = +2
Query: 5 YTPLLLRFVNXF*SLXCLFYVFKLXI 82
+ P+LL+ + ++ C+FYV+K+ +
Sbjct: 25 FNPILLKIILFLNTIVCIFYVYKIAL 50
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,403,813
Number of Sequences: 5004
Number of extensions: 67573
Number of successful extensions: 214
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 207
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 422462090
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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