Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P10_F_C09
         (879 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   389   e-109
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   386   e-108
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   196   3e-51
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...   159   5e-40
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom...    31   0.16 
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   8.1  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   8.1  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  389 bits (958), Expect = e-109
 Identities = 173/245 (70%), Positives = 206/245 (84%)
 Frame = +2

Query: 140 HVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVF 319
           HVGQAG QIGNACWELYCLEHGIQP+G M  +      D  F+TFFSETG GK+VPR+++
Sbjct: 8   HVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIY 67

Query: 320 VDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLAD 499
           VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD
Sbjct: 68  VDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIAD 127

Query: 500 QCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNS 679
            C+GLQGFL+FH            LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS
Sbjct: 128 NCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNS 187

Query: 680 ILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLXFDGA 859
           +LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASL F+G+
Sbjct: 188 VLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGS 247

Query: 860 LNVDL 874
           LNVDL
Sbjct: 248 LNVDL 252


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  386 bits (949), Expect = e-108
 Identities = 176/250 (70%), Positives = 208/250 (83%), Gaps = 5/250 (2%)
 Frame = +2

Query: 140 HVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHV 304
           HVGQAGVQIGNACWELYCLEHGI PDG  PT+     K     +D F TFFSETG GK V
Sbjct: 8   HVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFV 66

Query: 305 PRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRI 484
           PR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RI
Sbjct: 67  PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126

Query: 485 RKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVV 664
           R++AD C+GLQGFL+FH            LL+ERL+++YGKKS L+F++YPAPQVST+VV
Sbjct: 127 RRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVV 186

Query: 665 EPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASL 844
           EPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSITASL
Sbjct: 187 EPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASL 246

Query: 845 XFDGALNVDL 874
            F G+LNVDL
Sbjct: 247 RFAGSLNVDL 256


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  196 bits (478), Expect = 3e-51
 Identities = 96/243 (39%), Positives = 138/243 (56%)
 Frame = +2

Query: 146 GQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 325
           GQ G Q+G A W     EHG+   G      T     +  N +F+E   GK+VPRAV VD
Sbjct: 10  GQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVD 67

Query: 326 LEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQC 505
           LEP  +D V++G +  LF P+ +I G+  A N +A+GHYT G E+ D VLD +R+ A+ C
Sbjct: 68  LEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEAC 127

Query: 506 TGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSIL 685
             LQGF + H            LL+ ++  +Y  +    F++ PAP+ S  VVEPYN+ L
Sbjct: 128 DALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATL 187

Query: 686 TTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLXFDGALN 865
           + H  +E+SD  F +DNEA+  I    L I+ P+Y +LN L+  +++ +T S  F G LN
Sbjct: 188 SMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELN 247

Query: 866 VDL 874
            DL
Sbjct: 248 SDL 250


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score =  159 bits (386), Expect = 5e-40
 Identities = 86/246 (34%), Positives = 137/246 (55%), Gaps = 3/246 (1%)
 Frame = +2

Query: 146 GQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVD 325
           GQ G QIG+  W+  CLEHGI PDG + +  T   G D  + FF ++   +++PRA+ +D
Sbjct: 11  GQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILID 68

Query: 326 LEPTVVDEVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVLDRIRKLAD 499
           LEP VV+ + + TY  L++PE ++  K    A NN+A G Y+  + I + ++D I + AD
Sbjct: 69  LEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREAD 127

Query: 500 QCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYN 676
               L+GF + H             L+ERL+  Y KK    ++++P  Q VS  VV+PYN
Sbjct: 128 GSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYN 187

Query: 677 SILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLXFDG 856
           S+L       ++D   ++DN A+  I    L  + PT+   N+L+  ++S+ T +L + G
Sbjct: 188 SLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPG 247

Query: 857 ALNVDL 874
            +N DL
Sbjct: 248 YMNNDL 253


>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
           Msp1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 903

 Score = 31.5 bits (68), Expect = 0.16
 Identities = 16/43 (37%), Positives = 26/43 (60%)
 Frame = -3

Query: 868 HIQSAVEXQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 740
           H  ++ + Q+SSD  ++ +D T  VG+G AL   I + D +DG
Sbjct: 176 HRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +3

Query: 198 STASSLMARCPQTRPSGVETILSTLSSARPELAST 302
           ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +1

Query: 217 WPDAHRQDHRGWRRFFQHFLQR 282
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,449,679
Number of Sequences: 5004
Number of extensions: 69732
Number of successful extensions: 208
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 203
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)