BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P09_pT_P13
(600 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC29B5.03c |rpl26||60S ribosomal protein L26|Schizosaccharomyc... 159 3e-40
SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Ma... 29 0.52
SPAC57A7.07c |||homocysteine methyltransferase |Schizosaccharomy... 27 2.1
SPAC22F8.06 |pam1||20S proteasome component beta 6|Schizosacchar... 26 4.8
SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch... 25 6.4
SPAC27E2.12 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual 25 8.5
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 25 8.5
>SPBC29B5.03c |rpl26||60S ribosomal protein L26|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 126
Score = 159 bits (386), Expect = 3e-40
Identities = 73/122 (59%), Positives = 100/122 (81%)
Frame = -1
Query: 462 MKFNKQVTSSRRKNRKRHFSAPSHIRRVLMSSPLSKELRQKFNVKSMPIRKDDEVQVVRG 283
MKF++ VTSSRRK RK HF APS +RRVLMS+PLSKELR+++ ++S+P+R+DD++ V+RG
Sbjct: 1 MKFSRDVTSSRRKQRKAHFGAPSSVRRVLMSAPLSKELREQYKIRSLPVRRDDQITVIRG 60
Query: 282 HYKGQQVGKVMQVYRKKFVVYIERIQREKANGATAYVGIHPSKCVIVKLKMNKDRKAILD 103
KG++ GK+ VYRKKF++ IER+ REKANGA+A VGI SK VI KL ++KDRK ++
Sbjct: 61 SNKGRE-GKITSVYRKKFLLLIERVTREKANGASAPVGIDASKVVITKLHLDKDRKDLIV 119
Query: 102 RR 97
R+
Sbjct: 120 RK 121
>SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr
2|||Manual
Length = 918
Score = 29.1 bits (62), Expect = 0.52
Identities = 19/53 (35%), Positives = 28/53 (52%), Gaps = 3/53 (5%)
Frame = +1
Query: 175 ICCCTIGLFSLNPLNVYNKLFTIHLHHFA---NLLAFVVSTYNLNFIVFANRH 324
+ C +I LFS PL+ N+ F I+L F+ L ++ST + F F RH
Sbjct: 825 LTCISIPLFSFEPLSKKNR-FLINLFRFSFSFILYNLLISTSTVFFAGFFRRH 876
>SPAC57A7.07c |||homocysteine methyltransferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 308
Score = 27.1 bits (57), Expect = 2.1
Identities = 14/42 (33%), Positives = 24/42 (57%)
Frame = -1
Query: 576 SSVKRRPVRI*YDSL*GI*MNCFSISVLSRVVLAKSDRMKFN 451
SS+ + + I +DS+ GI +NCF +S+L + S + N
Sbjct: 196 SSIISKILSINHDSIWGIGVNCFHLSLLEPIAKMLSSLLPSN 237
>SPAC22F8.06 |pam1||20S proteasome component beta
6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 225
Score = 25.8 bits (54), Expect = 4.8
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -2
Query: 293 LYVDTTKANRLAK*CRCIVKSLLYTLRGF 207
LY D + A+ C C+V++LLY R F
Sbjct: 78 LYHDNHERKMSAQSCACMVRTLLYGKRFF 106
>SPBC16D10.05 |mok13||alpha-1,3-glucan synthase
Mok13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2358
Score = 25.4 bits (53), Expect = 6.4
Identities = 23/75 (30%), Positives = 31/75 (41%), Gaps = 2/75 (2%)
Frame = +2
Query: 41 FPRCIYPXSLPSAASLPFALRSRIALRSLFIFNLTITHFE--G*MPTYAVAPLAFSL*IL 214
FP +YP L + S P AL + L IF L ++E G +P + A L L +
Sbjct: 2090 FPHEVYPLGLIAVFSWPLAL-VMLLFAILLIFGLPDYYWESPGNIPAFYTALLRRKLVLW 2148
Query: 215 SMYTTNFLRYTCITL 259
T Y TL
Sbjct: 2149 FFVATILQNYWLSTL 2163
>SPAC27E2.12 |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
Length = 76
Score = 25.0 bits (52), Expect = 8.5
Identities = 12/30 (40%), Positives = 14/30 (46%)
Frame = +1
Query: 7 ICLIRPPWXVAVSSVYLPLXFAKCSQSALC 96
+CL R W A+ V PL C S LC
Sbjct: 15 VCLERFQWRGAIFLVCYPLYCVVCFVSVLC 44
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 25.0 bits (52), Expect = 8.5
Identities = 10/50 (20%), Positives = 24/50 (48%)
Frame = -3
Query: 517 ELLFYFGS*PCRFGEERQNEVQQAGDFLKKEKQEEAFQCSFTYKASVDVL 368
+ L + S C F ++ +E++ G +L + +F C + V+++
Sbjct: 3290 QYLEQYSSFLCEFHHQKFDEIEVPGQYLLHKDNNNSFSCIERFLPEVELI 3339
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,449,232
Number of Sequences: 5004
Number of extensions: 50772
Number of successful extensions: 135
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 262236260
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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