Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P09_pT_F13
(847 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 94 2e-20
SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces pombe... 43 6e-05
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 34 0.022
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 31 0.16
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 31 0.21
SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces ... 30 0.47
SPBC16A3.02c |||mitochondrial peptidase |Schizosaccharomyces pom... 29 0.63
SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyce... 28 1.9
SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz... 27 3.3
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 26 5.8
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 26 7.7
SPBC1215.01 |shy1||SURF-family protein Shy1|Schizosaccharomyces ... 26 7.7
>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 360
Score = 94.3 bits (224), Expect = 2e-20
Identities = 70/239 (29%), Positives = 105/239 (43%), Gaps = 18/239 (7%)
Frame = -1
Query: 847 EFSGTITDVGKK-SVFRNGQKVVVDPNRACSLCDFCRKGKYQYCLTAGINSTVGIWRDGG 671
E +G + +VGK S + G V V+P C LCD+CR G+Y C +T DG
Sbjct: 68 ESAGVVVEVGKGVSSLKPGDPVAVEPGCVCRLCDYCRSGRYNLCPHMEFAATPPY--DGT 125
Query: 670 WAQYVKVPQDQVYLLPDGVSTEQGGLCEPYSCVAHGYDRASPLLVGEKILIVGAGIIGNL 491
Y +D LP +S E+G L EP S H R + L G ++L++G G +G L
Sbjct: 126 LRTYYITTEDFCTKLPKQISVEEGALFEPMSVAVHAMTRGN-LKCGSRVLVMGCGTVGLL 184
Query: 490 WVTSLHQLGHRDVTVSEMNKVRLEIVNKLETGYRLVTP----------DVLEKEKQL--- 350
+ G D+ + + R+E K G + TP D ++ KQ
Sbjct: 185 MMAVAKAYGAIDIVAVDASPSRVEFAQKY-VGAKPFTPIAAKENESLPDYAQRYKQAIIE 243
Query: 349 ----YDVIIDCTGVGKVMEISFNYLRHGGKYVLFGCCPPTHQASINPFQIYDKELTIIG 185
+D +D TGVG + + L+ GG +V G P IN I + E+ ++G
Sbjct: 244 KYGEFDFAVDATGVGICIHTAVLALKRGGTFVQAGNGKPVIDFPIN--HIINYEINVLG 300
>SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 325
Score = 42.7 bits (96), Expect = 6e-05
Identities = 49/193 (25%), Positives = 82/193 (42%), Gaps = 3/193 (1%)
Frame = -1
Query: 700 STVGIWRDGGWAQYVKVPQDQVYLLPDGVS-TEQGGLCEPYSCVAHGYDRASPLLVGEKI 524
S + +DG A+Y +P+ +P +S TE + P++ R + +
Sbjct: 94 SELSFTKDGTHAEYCIIPEKAAVRMPSNLSFTEAASVGVPFTTAYLALSRGETK-GSDIV 152
Query: 523 LIVGA-GIIGNLWVTSLHQLGHRDVTVSEMNKVRLEIVNKLETGYRLVTPDVLEKEKQLY 347
L+VGA G +G+ G + +TVS + V ++ + V +++EK
Sbjct: 153 LVVGALGAVGSAVCQIAEDWGCKVITVSRSGSTDINTV--VDPELKRVH-ELVEK----V 205
Query: 346 DVIIDCTGVGKVMEISFNYLRHGGKYVLFGCCPP-THQASINPFQIYDKELTIIGVKINP 170
DV+ID G +M+ + N L GG+ + + S + QIY K L IIG
Sbjct: 206 DVVIDTVGDPLLMKSALNQLGIGGRLSYISAPKQGSIEFSYDMKQIYRKNLKIIGCNSLL 265
Query: 169 FSFPNALGWLKAM 131
S + LK M
Sbjct: 266 LSLVESNSLLKNM 278
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 34.3 bits (75), Expect = 0.022
Identities = 42/179 (23%), Positives = 73/179 (40%), Gaps = 23/179 (12%)
Frame = -1
Query: 796 GQKVVVDPNRACSLCDFCRKGKYQYCLTAG------IN------STVGIWR-----DGGW 668
G +VV+ + AC C FC++ +Y C T +N + G + G
Sbjct: 116 GDRVVIAFDLACGQCSFCKRHEYAACDTTNDSKLMDVNYGSHHSAIFGYTKLLGDVPGCQ 175
Query: 667 AQYVKVPQDQVYL--LPDGVSTEQGGLCEPYSCVAHGYDRASPLLVGEKILIVGAGIIGN 494
A+Y++VP ++ LPD + +G C + + G+ + I G G IG
Sbjct: 176 AEYIRVPFAEINCCKLPDDIPDSEGLFMSDVLCTSLHACTLGEVKKGDTVAIWGMGPIGL 235
Query: 493 LWVTSLHQLGHRDVTVSEMNKVRLEIVNKLETGYRLV----TPDVLEKEKQLYDVIIDC 329
LG V E+ R+E+ + + G+ ++ DV +K +L +DC
Sbjct: 236 YAGRWAQILGASKVIGIEVVPERIELARQ-KFGFTVIDRNEVSDVPKKIMELVSNGVDC 293
>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
Adh1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 350
Score = 31.5 bits (68), Expect = 0.16
Identities = 35/139 (25%), Positives = 58/139 (41%), Gaps = 5/139 (3%)
Frame = -1
Query: 847 EFSGTITDVGKKSV-FRNGQKVVVD-PNRACSLCDFCRKGKYQYCLTAGINSTVGIWRDG 674
E +G + VG + G +V V N +C C++C K + C ++ G DG
Sbjct: 70 EGAGVVVKVGAGVTRLKIGDRVGVKWMNSSCGNCEYCMKAEETICPHIQLS---GYTVDG 126
Query: 673 GWAQYVKVPQDQVYLLPDGVSTEQGG--LCEPYSCVAHGYDRASPLLVGEKILIVGA-GI 503
+ Y ++P+ V E +C +C + + S + GE I I GA G
Sbjct: 127 TFQHYCIANATHATIIPESVPLEVAAPIMCAGITC--YRALKESKVGPGEWICIPGAGGG 184
Query: 502 IGNLWVTSLHQLGHRDVTV 446
+G+L V + R V +
Sbjct: 185 LGHLAVQYAKAMAMRVVAI 203
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 31.1 bits (67), Expect = 0.21
Identities = 17/59 (28%), Positives = 25/59 (42%), Gaps = 1/59 (1%)
Frame = -1
Query: 847 EFSGTITDVGKKSV-FRNGQKVVVDPNRACSLCDFCRKGKYQYCLTAGINSTVGIWRDG 674
E +G + +G+ + R G V++ C C FCR GK C G+ DG
Sbjct: 70 EGAGIVESIGEGVINVRPGDHVILLYTPECKECKFCRSGKTNLCSKIRETQGRGLMPDG 128
>SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 444
Score = 29.9 bits (64), Expect = 0.47
Identities = 15/41 (36%), Positives = 26/41 (63%)
Frame = -1
Query: 529 KILIVGAGIIGNLWVTSLHQLGHRDVTVSEMNKVRLEIVNK 407
KILI+GAG +G + L G RD++V +M+ + + +N+
Sbjct: 46 KILIIGAGGLGCEILKDLALSGFRDLSVIDMDTIDITNLNR 86
>SPBC16A3.02c |||mitochondrial peptidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 347
Score = 29.5 bits (63), Expect = 0.63
Identities = 35/146 (23%), Positives = 57/146 (39%), Gaps = 6/146 (4%)
Frame = -1
Query: 676 GGWAQYVKVPQDQVYLLPDGVSTEQG-GLCEPYSCVAHGYDRASPLLVGEKILIVGA-GI 503
G A ++ V+ LPDGVS +G G R + G K++I GA G
Sbjct: 118 GSCATHIVTGDKDVWHLPDGVSFNEGAGFGIAGLTAWEVLVRQMKVKPGTKLVIEGASGG 177
Query: 502 IGNLWVTSLHQLGHRDVTVSEMNKVRLEIVNKLETGYRL--VTPDVLEKEKQL--YDVII 335
+G V L T+S L++ L + L +++E+ L YD +
Sbjct: 178 VGTFAVALAKALECEVTTISSTE--NLDLCKSLGATHTLDYKKDNLVERLADLGPYDFVF 235
Query: 334 DCTGVGKVMEISFNYLRHGGKYVLFG 257
DC + S +++ G + G
Sbjct: 236 DCVNDNVLYRASSKFVKPDGAFFGIG 261
>SPCC297.05 |||diacylglycerol binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 27.9 bits (59), Expect = 1.9
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +3
Query: 42 PALKSFRALSYSDRAYVFTPSFS*FTYL 125
PAL SFR+ ++S R YV +P + F L
Sbjct: 631 PALLSFRSSNFSKRPYVLSPILNGFLKL 658
>SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme
Fub2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 628
Score = 27.1 bits (57), Expect = 3.3
Identities = 13/41 (31%), Positives = 26/41 (63%)
Frame = -1
Query: 529 KILIVGAGIIGNLWVTSLHQLGHRDVTVSEMNKVRLEIVNK 407
K+L+VGAG IG + +L G ++V + +++ + L +N+
Sbjct: 27 KVLLVGAGGIGCELLKNLLMSGVKEVHIIDLDTIDLSNLNR 67
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 26.2 bits (55), Expect = 5.8
Identities = 15/32 (46%), Positives = 18/32 (56%), Gaps = 1/32 (3%)
Frame = -2
Query: 573 WRTATIALPLYLSERKSSSSE-PELLVTCGSR 481
W TA IAL Y E S+ E +LLV C S+
Sbjct: 1287 WNTALIALMTYYREAAISTPELLDLLVKCESK 1318
>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2310
Score = 25.8 bits (54), Expect = 7.7
Identities = 15/53 (28%), Positives = 28/53 (52%)
Frame = +3
Query: 48 LKSFRALSYSDRAYVFTPSFS*FTYLLPMAFSQPKALGKLNGLILTPMMVSSL 206
LKS +++++ + F P S +TY LP+ +AL + + P++ S L
Sbjct: 627 LKSLLLIAFANNGFAFHPKSSSWTYDLPVINRSFEALSSYD---IPPLLASLL 676
>SPBC1215.01 |shy1||SURF-family protein Shy1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 290
Score = 25.8 bits (54), Expect = 7.7
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = +2
Query: 188 DDGQFLVVNLEWIYRSLMSRRTASE*HVFPAVTKIIETDLHHFTD 322
DDG+ ++VN WI RS + +++ + P +IE L TD
Sbjct: 133 DDGRRILVNRGWIARS-FAEQSSRDPSSLPKGPVVIEGLLRQHTD 176
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,439,870
Number of Sequences: 5004
Number of extensions: 73833
Number of successful extensions: 244
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 236
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 243
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 418457710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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