Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P09_pT_E12
(914 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom... 333 2e-92
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 33 0.075
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 28 1.6
SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom... 28 1.6
SPBC16A3.13 |meu7|aah4|alpha-amylase homolog Aah4|Schizosaccharo... 27 2.8
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 27 3.7
SPBC27.01c |||secretory pathway protein Pga2 |Schizosaccharomyce... 27 4.9
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 27 4.9
SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa... 27 4.9
SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 26 6.5
>SPAC6G10.08 |idp1||isocitrate dehydrogenase
Idp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 418
Score = 333 bits (819), Expect = 2e-92
Identities = 156/250 (62%), Positives = 189/250 (75%), Gaps = 2/250 (0%)
Frame = -1
Query: 914 FKPXSGEAIKHV--VHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTI 741
F P + K V+E+ G+GVA++M+NTD SI FAHSSF+ AL +K PLYLSTKNTI
Sbjct: 166 FTPKGNPSAKETYNVYEFNGSGVAMSMYNTDDSIRGFAHSSFQMALQKKMPLYLSTKNTI 225
Query: 740 LKKYDGRFKDIFQDIYDREYKKQFEDAGIWYEHRLIDDMVAYAMKSEGGFVWACKNYDGD 561
LKKYDGRFKD FQ++Y+ +YK++FE+ G+WY+HRLIDDMVA A+KS GGFVWACKNYDGD
Sbjct: 226 LKKYDGRFKDTFQEVYESDYKQKFEELGLWYQHRLIDDMVAQAIKSNGGFVWACKNYDGD 285
Query: 560 VQSDSVAQGYGSLGLMTSVLICPDGKTVEAEAAHGTVTRHFRFYQQGKETSTNPIASIFA 381
V SD VAQ YGSLGLMTSVLI P+G+T E+EAAHGTV RH+ Y +GK+TSTN IASIFA
Sbjct: 286 VMSDVVAQAYGSLGLMTSVLIHPNGRTFESEAAHGTVQRHYMQYLKGKKTSTNSIASIFA 345
Query: 380 WTRGLLHRAKLDNNDALKNFAETLEKVCIETIESGIMTKDLAICIKGMNNVKRSDYYETF 201
WTRGL HR +LD N+ L FA LE C+ +E GIMTKDL + K N Y +TF
Sbjct: 346 WTRGLAHRGRLDGNERLVKFANALEHACVRCVEKGIMTKDLYLLSKSPNG-----YVDTF 400
Query: 200 EFMDKLAENL 171
EF+D + L
Sbjct: 401 EFLDAVKSEL 410
>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 362
Score = 32.7 bits (71), Expect = 0.075
Identities = 28/114 (24%), Positives = 51/114 (44%), Gaps = 1/114 (0%)
Frame = -1
Query: 791 FALDRKYPLYLSTKNTILKKYDGRFKDIFQDIYDREYKKQFEDAGIWYEHRLIDDMVAYA 612
+++ +K + + K+ ++ DG F++ + + A I + +++D MV
Sbjct: 183 YSIHKKPLVTIIHKSNVMSVTDGLFRESCRHAQSLDPSY----ASINVDEQIVDSMVYRL 238
Query: 611 MKSEGGF-VWACKNYDGDVQSDSVAQGYGSLGLMTSVLICPDGKTVEAEAAHGT 453
+ F V N GD+ SD A GSLGL+ S + V +E HG+
Sbjct: 239 FREPECFDVVVAPNLYGDILSDGAASLIGSLGLVPSANV--GDNFVMSEPVHGS 290
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 28.3 bits (60), Expect = 1.6
Identities = 18/94 (19%), Positives = 40/94 (42%)
Frame = -1
Query: 902 SGEAIKHVVHEYKGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYDG 723
SG+ + H V Y+G + A+ D + D K ++R Y + + I++
Sbjct: 155 SGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKE 214
Query: 722 RFKDIFQDIYDREYKKQFEDAGIWYEHRLIDDMV 621
+ + D +++E + + + + + L D V
Sbjct: 215 KLCYVALD-FEQELQTAAQSSSLEKSYELPDGQV 247
>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 28.3 bits (60), Expect = 1.6
Identities = 51/185 (27%), Positives = 77/185 (41%), Gaps = 6/185 (3%)
Frame = -1
Query: 752 KNTILKKYDGRF----KDIFQDIYDREYKKQFEDAGIWYEHRLIDDMVAYAMKSEGGFVW 585
K TI++ DG F K++ + D E +++ D +++ D V Y V
Sbjct: 213 KATIMRMADGLFLECAKELAPEYPDIELREEILDNACL---KIVTDPVPY-----NNTVM 264
Query: 584 ACKNYDGDVQSDSVAQGYGSLGLMTSVLICPDGKTVEAEAAHGTVTRHFRFYQQGKETST 405
N GD+ SD A G LGL S I + EA HGT GK +
Sbjct: 265 VMPNLYGDIVSDMCAGLIGGLGLTPSGNI--GNQASIFEAVHGTAPD-----IAGKGLA- 316
Query: 404 NPIASIFAWTRGLLHRAKLDNNDALKNFAETLEKVCIETIESG--IMTKDLAICIKGMNN 231
NP A + + L H ++ ND +A+ +E +T+ + TKDL NN
Sbjct: 317 NPTALLLSSVMMLKH---MNLND----YAKRIESAIFDTLANNPDARTKDLG---GKSNN 366
Query: 230 VKRSD 216
V+ +D
Sbjct: 367 VQYTD 371
>SPBC16A3.13 |meu7|aah4|alpha-amylase homolog
Aah4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 774
Score = 27.5 bits (58), Expect = 2.8
Identities = 19/57 (33%), Positives = 25/57 (43%), Gaps = 1/57 (1%)
Frame = +2
Query: 530 HSPEQHYQIVRLHHNFCRPRQIHPLIS*HKPPYHQLACVHTRYQHPQTVSCT-PCHK 697
H P +H + H F RP + HKP H+ C H R+ P + T P HK
Sbjct: 278 HKPWKHEEHCH-HGKFPRPIPHNGTKPDHKPWKHEEHCHHGRFPRPVPHNGTKPDHK 333
Score = 27.1 bits (57), Expect = 3.7
Identities = 18/57 (31%), Positives = 25/57 (43%), Gaps = 1/57 (1%)
Frame = +2
Query: 530 HSPEQHYQIVRLHHNFCRPRQIHPLIS*HKPPYHQLACVHTRYQHPQTVSCT-PCHK 697
H P +H + H F RP + HKP H+ C H ++ P + T P HK
Sbjct: 361 HKPWKHEEHCH-HGKFLRPVPHNVTKPDHKPWKHEEHCHHGKFPRPVPHNGTKPDHK 416
>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
Prp43|Schizosaccharomyces pombe|chr 2|||Manual
Length = 735
Score = 27.1 bits (57), Expect = 3.7
Identities = 17/73 (23%), Positives = 33/73 (45%)
Frame = -1
Query: 461 HGTVTRHFRFYQQGKETSTNPIASIFAWTRGLLHRAKLDNNDALKNFAETLEKVCIETIE 282
H T+ + Y+ G+ T+ + W L HRA + ++ K T+E+ +E I
Sbjct: 561 HLTLLNVYHAYKSGEGTAD------WCWNHFLSHRALISADNVRKQLRRTMERQEVELIS 614
Query: 281 SGIMTKDLAICIK 243
+ K+ + I+
Sbjct: 615 TPFDDKNYYVNIR 627
>SPBC27.01c |||secretory pathway protein Pga2 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 132
Score = 26.6 bits (56), Expect = 4.9
Identities = 18/57 (31%), Positives = 24/57 (42%), Gaps = 1/57 (1%)
Frame = -1
Query: 491 DGKTVEAEAAHGTVTR-HFRFYQQGKETSTNPIASIFAWTRGLLHRAKLDNNDALKN 324
DG T++ E HGT + H F +E NP A F W R + + KN
Sbjct: 56 DG-TLDPEMTHGTKPKEHGEFDTDDEEEEENPDAE-FRWGYSARRRIRKQREEYFKN 110
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 26.6 bits (56), Expect = 4.9
Identities = 17/63 (26%), Positives = 29/63 (46%), Gaps = 4/63 (6%)
Frame = -1
Query: 653 WYEH-RLIDDMVAYAMKSEGGFVWACKNYDGDVQSD---SVAQGYGSLGLMTSVLICPDG 486
W H DD+ + G V+ KNYDGDV++D + + G+ + + P+G
Sbjct: 794 WLGHPSYFDDLTSLDPNLYEGLVFL-KNYDGDVENDMALNFTVVHEEFGVRNVIDLIPNG 852
Query: 485 KTV 477
+
Sbjct: 853 SNI 855
>SPCC550.03c |||RNA helicase involved in mRNA
catabolism|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1213
Score = 26.6 bits (56), Expect = 4.9
Identities = 16/57 (28%), Positives = 28/57 (49%)
Frame = -1
Query: 824 SIIDFAHSSFKFALDRKYPLYLSTKNTILKKYDGRFKDIFQDIYDREYKKQFEDAGI 654
S+ AH+S + +Y + L+ K+ Y K + + R++K +FED GI
Sbjct: 297 SVFVAAHTSAGKTVVAEYAIALAQKHMTKAIYTSPIKALSNQKF-RDFKHKFEDVGI 352
>SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase
Cdc25|Schizosaccharomyces pombe|chr 1|||Manual
Length = 596
Score = 26.2 bits (55), Expect = 6.5
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = -1
Query: 755 TKNTILKKYDGRFKDIFQDIYDREYKKQFEDAG 657
T+ T+L DG+FKDIF + + ++E G
Sbjct: 414 TQETLLGLLDGKFKDIFDKCIIIDCRFEYEYLG 446
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,540,843
Number of Sequences: 5004
Number of extensions: 71850
Number of successful extensions: 187
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 181
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 464508080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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