Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P09_pT_D12
(772 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 251 7e-68
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 251 7e-68
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 176 2e-45
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 97 3e-21
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 45 1e-05
SPCP31B10.05 |||tyrosyl-DNA phosphodiesterase |Schizosaccharomyc... 29 0.97
SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+, L-... 27 2.2
SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein |Schizo... 26 5.2
SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces pom... 26 5.2
SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosacchar... 25 9.1
SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomy... 25 9.1
SPBC3H7.09 |mug142||palmitoyltransferase|Schizosaccharomyces pom... 25 9.1
>SPCP31B10.07 |eft202||translation elongation factor 2
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 842
Score = 251 bits (615), Expect = 7e-68
Identities = 115/176 (65%), Positives = 131/176 (74%)
Frame = -2
Query: 768 FQWAXKEGVMAEENLRGVRFNXYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLME 589
F WA KEG M EENLR RFN DV LH DAIHRGGGQIIPT RR +YA L A P + E
Sbjct: 667 FAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQE 726
Query: 588 PVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRS 409
PV+L EIQ E A+GGIY VLN++RGHVF E Q GTP++ +KAYLPVNESFGFT +LR
Sbjct: 727 PVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFTGELRQ 786
Query: 408 NTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRKGLKEGLPDLTQYLDKL 241
T GQAFPQ VFDHW + GDP +P SKP +V E RKRKGLKE +PD T+Y D+L
Sbjct: 787 ATAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRKGLKENVPDYTEYYDRL 842
>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
elongation factor 2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 842
Score = 251 bits (615), Expect = 7e-68
Identities = 115/176 (65%), Positives = 131/176 (74%)
Frame = -2
Query: 768 FQWAXKEGVMAEENLRGVRFNXYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLME 589
F WA KEG M EENLR RFN DV LH DAIHRGGGQIIPT RR +YA L A P + E
Sbjct: 667 FAWASKEGPMFEENLRSCRFNILDVVLHADAIHRGGGQIIPTARRVVYASTLLASPIIQE 726
Query: 588 PVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRS 409
PV+L EIQ E A+GGIY VLN++RGHVF E Q GTP++ +KAYLPVNESFGFT +LR
Sbjct: 727 PVFLVEIQVSENAMGGIYSVLNKKRGHVFSEEQRVGTPLYNIKAYLPVNESFGFTGELRQ 786
Query: 408 NTGGQAFPQCVFDHWQVLPGDPCEPQSKPYNVVQETRKRKGLKEGLPDLTQYLDKL 241
T GQAFPQ VFDHW + GDP +P SKP +V E RKRKGLKE +PD T+Y D+L
Sbjct: 787 ATAGQAFPQLVFDHWSPMSGDPLDPTSKPGQIVCEARKRKGLKENVPDYTEYYDRL 842
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 176 bits (429), Expect = 2e-45
Identities = 88/188 (46%), Positives = 119/188 (63%), Gaps = 12/188 (6%)
Frame = -2
Query: 771 GFQWAXKEGVMAEENLRGVRFNXYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLM 592
GFQW +EG + +E +R V F DV L + I+RGGGQIIPT RR Y+ LTA PRLM
Sbjct: 782 GFQWGTREGPLCDETIRNVNFRLMDVVLAPEQIYRGGGQIIPTARRVCYSSFLTASPRLM 841
Query: 591 EPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLR 412
EPVY+ E+ P ++ IY +L RRRGHV ++ G+P+++V+A +PV +S GF DLR
Sbjct: 842 EPVYMVEVHAPADSLPIIYDLLTRRRGHVLQDIPRPGSPLYLVRALIPVIDSCGFETDLR 901
Query: 411 SNTGGQAFPQCVFDHWQVLPGDPCEPQSKP------------YNVVQETRKRKGLKEGLP 268
+T GQA Q VFDHWQV+PGDP + KP + + +TR+RKGL E +
Sbjct: 902 VHTQGQAMCQMVFDHWQVVPGDPLDKSIKPKPLEPARGSDLARDFLIKTRRRKGLVEDV- 960
Query: 267 DLTQYLDK 244
T+Y D+
Sbjct: 961 STTRYFDQ 968
>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1000
Score = 96.7 bits (230), Expect = 3e-21
Identities = 43/106 (40%), Positives = 64/106 (60%)
Frame = -2
Query: 660 GQIIPTTRRCLYACLLTAQPRLMEPVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAG 481
GQ+I + + L PRLM +Y C++Q +G +YGV+++RRG V +E G
Sbjct: 841 GQVISVVKESIRHGFLGWSPRLMLAMYSCDVQATSEVLGRVYGVVSKRRGRVIDEEMKEG 900
Query: 480 TPMFIVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQVLPGDP 343
TP FIVKA +PV ESFGF ++ T G A+PQ +F +++L +P
Sbjct: 901 TPFFIVKALIPVVESFGFAVEILKRTSGAAYPQLIFHGFEMLDENP 946
>SPBC1306.01c ||SPBC409.22c|translation elongation factor
G|Schizosaccharomyces pombe|chr 2|||Manual
Length = 770
Score = 45.2 bits (102), Expect = 1e-05
Identities = 30/125 (24%), Positives = 55/125 (44%)
Frame = -2
Query: 768 FQWAXKEGVMAEENLRGVRFNXYDVTLHTDAIHRGGGQIIPTTRRCLYACLLTAQPRLME 589
F A K+G + ++ RF D H ++ T L A P ++E
Sbjct: 619 FYEALKKGFLIGHPIKNCRFVLEDGAYHPVDSSELAFRL--ATISAFRTAFLQANPMVLE 676
Query: 588 PVYLCEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTPMFIVKAYLPVNESFGFTADLRS 409
P+ I P GG+ G L++R+ + + F ++A +P+N F +++D+R+
Sbjct: 677 PIMNVSITAPVEHQGGVIGNLDKRKATIVDSD--TDEDEFTLQAEVPLNSMFSYSSDIRA 734
Query: 408 NTGGQ 394
T G+
Sbjct: 735 LTKGK 739
>SPCP31B10.05 |||tyrosyl-DNA phosphodiesterase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 536
Score = 28.7 bits (61), Expect = 0.97
Identities = 30/139 (21%), Positives = 55/139 (39%), Gaps = 4/139 (2%)
Frame = -3
Query: 758 PXRKELWLKRICVVLDSTXMM*HSILMPSIEV-VAKSFQQLEDACTHVC*LLS--PVSWS 588
P K+ W CV L+ H+ + P+++V + + D+ + ++ PV+
Sbjct: 50 PQLKQTWQFNFCVDLNFLLENMHASVFPTVDVRITHGYDSKSDSLARLTAQMNHCPVNVK 109
Query: 587 LYIFVKFSVLK*LWXXXXXXXXXXXXTFSKSPRWQVHLCSL*RPTYLSMSRSVL-LPICV 411
LY SV +W S + +H +L P ++ MS+++ P+
Sbjct: 110 LY-----SVYVPMWGTHHSKIMVNFFK-DDSCQIVIHTANLVEPDWIGMSQAIFKTPLLY 163
Query: 410 PTPADRPSRSAYSTIGRSS 354
P D S S+ G S
Sbjct: 164 PKANDSLSTSSVPEYGNPS 182
>SPAC227.18 |lys3|SPAC2F7.01|saccharopine dehydrogenase [NAD+,
L-lysine forming] |Schizosaccharomyces pombe|chr
1|||Manual
Length = 368
Score = 27.5 bits (58), Expect = 2.2
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +2
Query: 641 VVGMIWPPPLWMASVWSVTSXMLNLTPRKFSSAITPSLXA 760
V G+ PPPL + S+ + + + + FS A+ PSL A
Sbjct: 305 VKGVTTPPPLEVISIDHLPTLLPRESSEAFSEALIPSLLA 344
>SPAC1006.08 |etd1||ethanol-hypersensitive mutant protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 391
Score = 26.2 bits (55), Expect = 5.2
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Frame = +1
Query: 586 RLHETGL-SS*QTCVQASSSCWNDLATTSMDGISMECYIIXVESNTTQI 729
++ ET L ++ Q + CW++L+TTS + + II + TT I
Sbjct: 191 QIQETSLGATTQEDESSDGICWDELSTTSPESSKVSEPIIQDNTQTTHI 239
>SPAC227.16c |||GINS complex subunit Psf3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 166
Score = 26.2 bits (55), Expect = 5.2
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +2
Query: 665 PLWMASVWSVTSXMLNLTPRKFSSAITPSLXAHWN 769
P W+A V ++ S + P FSS + +L A+ N
Sbjct: 43 PFWLAEVLAINSFVSIHMPAPFSSVVRNALKANPN 77
>SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 232
Score = 25.4 bits (53), Expect = 9.1
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = -2
Query: 369 HWQVLPGDPCEPQSKPYNVVQETRKRK 289
H+Q L GDP + PY VQ RK
Sbjct: 78 HFQNLSGDPVSQMATPYPPVQIELMRK 104
>SPBC1734.15 |rsc4|brd1|RSC complex subunit Rsc4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 25.4 bits (53), Expect = 9.1
Identities = 17/67 (25%), Positives = 29/67 (43%), Gaps = 1/67 (1%)
Frame = -2
Query: 651 IPTTRRCLYACLLTAQPRLMEPVYL-CEIQCPEVAVGGIYGVLNRRRGHVFEESQVAGTP 475
IP T RC+ + + + P+L P L + CP + + V+ H F + P
Sbjct: 386 IPNTERCILSAFICSPPQLPLPNPLRMYLPCPSLNSTEV-SVITLAPQHSFLNIVINLNP 444
Query: 474 MFIVKAY 454
+K+Y
Sbjct: 445 ALALKSY 451
>SPBC3H7.09 |mug142||palmitoyltransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 350
Score = 25.4 bits (53), Expect = 9.1
Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 3/39 (7%)
Frame = +3
Query: 126 SHLRVCFNMMRNM---CC*K*TCVKRFPYRYVF*FALTM 233
SH +C N + + C TC+ R YRY F F L++
Sbjct: 196 SHCHLCDNCVEYLDHHCIWLNTCIGRRNYRYYFIFLLSV 234
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,255,410
Number of Sequences: 5004
Number of extensions: 69063
Number of successful extensions: 154
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 153
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 371330890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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