Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P09_F_O09
(808 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|... 345 3e-96
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase... 345 6e-96
SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces pom... 26 5.5
SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 26 5.5
SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 26 5.5
SPAC1B1.04c |||poly|Schizosaccharomyces pombe|chr 1|||Manual 26 7.2
SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 26 7.2
SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase Ubp10|Schizosa... 25 9.6
SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomy... 25 9.6
>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 335
Score = 345 bits (849), Expect = 3e-96
Identities = 170/237 (71%), Positives = 191/237 (80%), Gaps = 1/237 (0%)
Frame = +3
Query: 99 KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 275
K+GINGFGRIGR+VLR +I G QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE +
Sbjct: 5 KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64
Query: 276 GFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAP 455
G LV++G+ I V +ERDP I W +GAEYV+ESTGVFTT + ASAHL+GGAK+VIISAP
Sbjct: 65 GKLVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAP 124
Query: 456 SADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXX 635
S DAPMFVVGVNLE ++PS KVISNASCTTNCLAPLAKVI+D F I EGLM
Sbjct: 125 SKDAPMFVVGVNLEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGLMTTVHATTAT 184
Query: 636 XXXXDGPSGKLWRDGRGAQQNIIPASTGAAKAVGKVIPALNGKLTGMAFRVPVANVS 806
DGPS K WR GRGA NIIP+STGAAKAVGKVIPALNGKLTGMAFRVP +VS
Sbjct: 185 QKTVDGPSKKDWRGGRGASANIIPSSTGAAKAVGKVIPALNGKLTGMAFRVPTPDVS 241
>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 336
Score = 345 bits (847), Expect = 6e-96
Identities = 169/237 (71%), Positives = 191/237 (80%), Gaps = 1/237 (0%)
Frame = +3
Query: 99 KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 275
K+GINGFGRIGR+VLR A + K QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++D
Sbjct: 5 KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64
Query: 276 GFLVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAP 455
G LV++GN I V +ERDP I W +GA+YV+ESTGVFTT + ASAHL+GGAK+VIISAP
Sbjct: 65 GKLVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAP 124
Query: 456 SADAPMFVVGVNLEAYDPSFKVISNASCTTNCLAPLAKVIHDNFEIVEGLMXXXXXXXXX 635
S DAPM+VVGVN E ++PS KVISNASCTTNCLAPLAKVI+D F I EGLM
Sbjct: 125 SKDAPMYVVGVNEEKFNPSEKVISNASCTTNCLAPLAKVINDTFGIEEGLMTTVHATTAT 184
Query: 636 XXXXDGPSGKLWRDGRGAQQNIIPASTGAAKAVGKVIPALNGKLTGMAFRVPVANVS 806
DGPS K WR GRGA NIIP+STGAAKAVGKVIPALNGKLTGMAFRVP +VS
Sbjct: 185 QKTVDGPSKKDWRGGRGASANIIPSSTGAAKAVGKVIPALNGKLTGMAFRVPTPDVS 241
>SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 381
Score = 26.2 bits (55), Expect = 5.5
Identities = 16/62 (25%), Positives = 29/62 (46%), Gaps = 1/62 (1%)
Frame = -3
Query: 464 ISTGS*YNNFFSTSLQVSRCFICSGKDTSRLYNIFSPSFSPRNGLRVPF*E-NSNFVTIN 288
+ +G +N +S + C +C G + ++ +P F R +R+P N N TI
Sbjct: 153 VCSGQGFNPKYSADKAIESCPVCGGSGFRVIEHMIAPGF--RQQMRMPCNACNGNGRTIK 210
Query: 287 NK 282
+K
Sbjct: 211 HK 212
>SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein
Ubi3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 26.2 bits (55), Expect = 5.5
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +2
Query: 23 LHLVLNVRGGGQLFNKFSITCRXHVKN 103
LHLVL +RGGG+ K + T +K+
Sbjct: 67 LHLVLRLRGGGKKRKKKTYTTPKKIKH 93
>SPAC589.10c |||ribomal-ubiquitin fusion protein
Ubi5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 150
Score = 26.2 bits (55), Expect = 5.5
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +2
Query: 23 LHLVLNVRGGGQLFNKFSITCRXHVKN 103
LHLVL +RGGG+ K + T +K+
Sbjct: 67 LHLVLRLRGGGKKRKKKTYTTPKKIKH 93
>SPAC1B1.04c |||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 604
Score = 25.8 bits (54), Expect = 7.2
Identities = 10/31 (32%), Positives = 19/31 (61%)
Frame = -3
Query: 350 FSPRNGLRVPF*ENSNFVTINNKESILNLNT 258
FSP V E+++ +++N +S+ NLN+
Sbjct: 67 FSPSKATTVSLKESASLTSLSNNKSVSNLNS 97
>SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 382
Score = 25.8 bits (54), Expect = 7.2
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +2
Query: 23 LHLVLNVRGGGQLFNK 70
LHLVL +RGG Q+F K
Sbjct: 67 LHLVLRLRGGMQIFVK 82
Score = 25.8 bits (54), Expect = 7.2
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +2
Query: 23 LHLVLNVRGGGQLFNK 70
LHLVL +RGG Q+F K
Sbjct: 143 LHLVLRLRGGMQIFVK 158
Score = 25.8 bits (54), Expect = 7.2
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +2
Query: 23 LHLVLNVRGGGQLFNK 70
LHLVL +RGG Q+F K
Sbjct: 219 LHLVLRLRGGMQIFVK 234
Score = 25.8 bits (54), Expect = 7.2
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +2
Query: 23 LHLVLNVRGGGQLFNK 70
LHLVL +RGG Q+F K
Sbjct: 295 LHLVLRLRGGMQIFVK 310
>SPBC577.07 |ubp10||ubiquitin C-terminal hydrolase
Ubp10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 502
Score = 25.4 bits (53), Expect = 9.6
Identities = 14/37 (37%), Positives = 19/37 (51%)
Frame = +3
Query: 114 GFGRIGRLVLRASIEKGAQVVAINDPFIGLDYMVYLF 224
GF RI +R EKG QVV D I + + +L+
Sbjct: 298 GFVRIESQKIRQHAEKGEQVVFTGDRVIQTNVVPFLY 334
>SPAC31A2.05c |mis4||cohesin loading factor Mis4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1583
Score = 25.4 bits (53), Expect = 9.6
Identities = 17/50 (34%), Positives = 27/50 (54%), Gaps = 3/50 (6%)
Frame = -3
Query: 305 NFVTINNKESILNLNTALKTAMGGIILEKINHIVK---TDERVIYSDHLS 165
+F + N+ESIL+L L + + E I++IV+ +D R HLS
Sbjct: 338 SFKKLQNEESILHLLNILHSIFEYTVPEAIDNIVQSKTSDARTSEIQHLS 387
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,426,453
Number of Sequences: 5004
Number of extensions: 73216
Number of successful extensions: 222
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 211
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 220
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 392429240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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