Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P09_F_I21
(790 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 46 5e-06
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 44 2e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 35 0.015
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 32 0.11
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 31 0.14
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 29 0.57
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 29 0.57
SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|c... 29 1.0
SPCC74.02c |||mRNA cleavage and polyadenylation specificity fact... 27 2.3
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 27 3.1
SPBPB10D8.01 |||cysteine transporter |Schizosaccharomyces pombe|... 27 3.1
SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces pombe... 27 4.0
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 27 4.0
SPBC582.10c |||ATP-dependent DNA helicase Rhp16b |Schizosaccharo... 26 5.4
SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharo... 25 9.4
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 25 9.4
SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr 2... 25 9.4
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 46.4 bits (105), Expect = 5e-06
Identities = 25/89 (28%), Positives = 46/89 (51%), Gaps = 1/89 (1%)
Frame = +1
Query: 505 DDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSES 684
D DG+ + E ++ G +E +++ ++ D D +G+I EFL + M ++
Sbjct: 24 DQDGN--ITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDT 81
Query: 685 -RRNIVXQAFKKLDKTGDGAITIDDIKGV 768
V +AFK DK G+G IT++++ V
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHV 110
Score = 37.1 bits (82), Expect = 0.003
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +1
Query: 493 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEF 651
F+ D DG+ + EE + + G L++ E ++ + DTD G I+ +EF
Sbjct: 91 FKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEF 143
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 44.0 bits (99), Expect = 2e-05
Identities = 33/144 (22%), Positives = 66/144 (45%), Gaps = 6/144 (4%)
Frame = +1
Query: 376 EELMQKSARAMTQATDPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEFLYGI 555
E + ++ + + +++ L + AS L R+F +D+DG ++ +EF+ +
Sbjct: 24 ERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLA-SRLFSVVDEDGGGDVDFQEFINSL 82
Query: 556 KETGLELNKSEAEEL-FSQFDTDNSGSISLDEFLIKIRPPMSESRR-----NIVXQAFKK 717
+ NK E + F +D D G IS E + ++ + + R IV + +
Sbjct: 83 SVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDKTIME 142
Query: 718 LDKTGDGAITIDDIKGVYSVDSXT 789
+DK DG I+ ++ K + S + T
Sbjct: 143 VDKDRDGKISFEEFKDIVSGSNVT 166
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 34.7 bits (76), Expect = 0.015
Identities = 18/97 (18%), Positives = 41/97 (42%), Gaps = 1/97 (1%)
Frame = +1
Query: 481 LGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFL-I 657
+ F+ D D ++ E ++ G KSE ++ FD G + +++F+ +
Sbjct: 39 INEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRV 98
Query: 658 KIRPPMSESRRNIVXQAFKKLDKTGDGAITIDDIKGV 768
+ + +AF+ D G I++ +++ V
Sbjct: 99 MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRV 135
Score = 33.5 bits (73), Expect = 0.035
Identities = 16/62 (25%), Positives = 29/62 (46%)
Frame = +1
Query: 571 ELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVXQAFKKLDKTGDGAITI 750
E + + E F FD+D +I E +R + ++ V + + DKTG G + +
Sbjct: 33 EEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQM 92
Query: 751 DD 756
+D
Sbjct: 93 ED 94
Score = 33.1 bits (72), Expect = 0.047
Identities = 16/56 (28%), Positives = 25/56 (44%)
Frame = +1
Query: 487 RIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFL 654
R F DDD + ++ KE ++ E E + +FD D G I+ EF+
Sbjct: 114 RAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDGEINEQEFI 169
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 31.9 bits (69), Expect = 0.11
Identities = 23/79 (29%), Positives = 37/79 (46%), Gaps = 1/79 (1%)
Frame = +1
Query: 526 LNKEEFLYGIKETGLELNKSE-AEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVX 702
LNK EF K+ + S AE +F+ FD D +G I EF+ + + +
Sbjct: 43 LNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFICALSVTSRGELNDKLI 102
Query: 703 QAFKKLDKTGDGAITIDDI 759
AF+ D +G I+ D++
Sbjct: 103 WAFQLYDLDNNGLISYDEM 121
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 31.5 bits (68), Expect = 0.14
Identities = 22/96 (22%), Positives = 40/96 (41%), Gaps = 2/96 (2%)
Frame = +1
Query: 478 GLGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLI 657
G+ ++FR + S +LN+ EF + GL + E LF + G ++ + F
Sbjct: 488 GITKVFRHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEG-VTYERFTE 546
Query: 658 KIRPPMSESRRNIVXQAFKKLDKTGDGA--ITIDDI 759
+ + + R+ Q DG +T DD+
Sbjct: 547 IVMEELED--RDSARQVLYAFCDVADGKSYVTSDDL 580
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 29.5 bits (63), Expect = 0.57
Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 1/58 (1%)
Frame = +2
Query: 581 KVKPKNSSVNSTQTIVAQSVLMNSLLKSVLLCRNRVVT-LXNKHSRSLTRLVTVQSQL 751
K+ KN+ S + + ++ L L L + +++ L NK S TRL +QSQL
Sbjct: 765 KLNKKNADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEESTRLQELQSQL 822
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 29.5 bits (63), Expect = 0.57
Identities = 21/75 (28%), Positives = 34/75 (45%), Gaps = 1/75 (1%)
Frame = +1
Query: 538 EFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLIKIRP-PMSESRRNIVXQAFK 714
E+ G+ + + S LF +FD +GS+SL + + I + RNI F+
Sbjct: 564 EWAKGLDAAAINNSSSFLRHLFLRFDKSMTGSLSLQDLVSGIAELKFRDVMRNISF-IFE 622
Query: 715 KLDKTGDGAITIDDI 759
D GDG + D+
Sbjct: 623 LYDFNGDGFMDKPDV 637
>SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 488
Score = 28.7 bits (61), Expect = 1.0
Identities = 15/43 (34%), Positives = 23/43 (53%)
Frame = +1
Query: 265 NYENSPKVKAEMESSGKPKTSSNIAWHRPMSAGSVQEEELMQK 393
N+ N + E S KPK S ++W+ + GS +E EL+ K
Sbjct: 41 NFNNQERSDLEHIHS-KPKKDSLLSWNILLKKGSYKENELLAK 82
>SPCC74.02c |||mRNA cleavage and polyadenylation specificity factor
complex associated protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 710
Score = 27.5 bits (58), Expect = 2.3
Identities = 21/62 (33%), Positives = 30/62 (48%), Gaps = 3/62 (4%)
Frame = +1
Query: 268 YENSPKVKAEMESSGKPKTSSNIAWHR-PMSAGSVQEEEL--MQKSARAMTQATDPLEKL 438
Y N+P + SG +S N A H P S V + L ++ +R ++DPLE L
Sbjct: 138 YVNNPSHTSRNPDSG---SSLNTASHEVPSSKSDVNVQMLARLKSKSRQKISSSDPLEDL 194
Query: 439 RL 444
RL
Sbjct: 195 RL 196
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 27.1 bits (57), Expect = 3.1
Identities = 14/30 (46%), Positives = 18/30 (60%), Gaps = 2/30 (6%)
Frame = +1
Query: 568 LELNKSEA--EELFSQFDTDNSGSISLDEF 651
L LN S EE F + D D+SG +S +EF
Sbjct: 326 LHLNASMEFLEETFQKADADHSGKLSFEEF 355
>SPBPB10D8.01 |||cysteine transporter |Schizosaccharomyces pombe|chr
2|||Manual
Length = 552
Score = 27.1 bits (57), Expect = 3.1
Identities = 14/47 (29%), Positives = 24/47 (51%)
Frame = +3
Query: 72 RIVTAVPRLYIIEAISINRY*PIVCDTIV*KTIIQQLVIDVSWLFSF 212
R +T RLY ++ + + + I T +I+ L V+WLF+F
Sbjct: 276 RFLTKKERLYTVDRVRRSNHSGIESKTFKKYQVIEALKDPVTWLFTF 322
>SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 746
Score = 26.6 bits (56), Expect = 4.0
Identities = 10/34 (29%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -1
Query: 550 HKGIL-LCSVVYFHHHPCDGKFFQVPRSLKHLET 452
H+G++ +C++VY KF + P++++ L T
Sbjct: 686 HRGLVCICNIVYSKDQEIFNKFIKTPKAVETLRT 719
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 26.6 bits (56), Expect = 4.0
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +1
Query: 673 MSESRRNIVXQAFKKLDKTGDGAITIDDIK 762
++ S+ + +AF LDK GDG I +D+K
Sbjct: 42 LTSSQIQELKEAFALLDKDGDGNIGREDVK 71
>SPBC582.10c |||ATP-dependent DNA helicase Rhp16b
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 830
Score = 26.2 bits (55), Expect = 5.4
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = -2
Query: 297 FSFYFWRILVVEASFILN 244
F+FY+WRI++ EA I N
Sbjct: 374 FAFYWWRIILDEAHTIKN 391
>SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1369
Score = 25.4 bits (53), Expect = 9.4
Identities = 10/31 (32%), Positives = 21/31 (67%)
Frame = -2
Query: 282 WRILVVEASFILNSFLFLFRTMA*KKTAN*H 190
W+I+V+ SF++ ++L +F + +K +N H
Sbjct: 1191 WKIVVMTVSFLVGAWLIIFYVL--RKLSNKH 1219
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 25.4 bits (53), Expect = 9.4
Identities = 10/31 (32%), Positives = 16/31 (51%)
Frame = +2
Query: 434 NCACFACLEVLQGSWDLEEFSVAWMMMEVNY 526
N F +E + +D EEFSV W + + +
Sbjct: 41 NTTAFWFVEFTESKYDKEEFSVIWNEVSMEF 71
>SPBC337.07c |||carboxypeptidase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 497
Score = 25.4 bits (53), Expect = 9.4
Identities = 25/101 (24%), Positives = 43/101 (42%), Gaps = 5/101 (4%)
Frame = +1
Query: 364 SVQEEELMQKSARAMTQATDPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEF 543
S Q E + R M L +L + ++ ILGL R D+G K+ NK+
Sbjct: 182 SYQNLESINSWLRLMASLYKDLSELVPVGITAEGRTILGLKLNGRHPSDNGEKIRNKKVI 241
Query: 544 L-----YGIKETGLELNKSEAEELFSQFDTDNSGSISLDEF 651
+ + + G+ A +L +++D+D LD+F
Sbjct: 242 IIQGGSHAREWIGIPSVCYAAWQLLAKYDSDGHVRKLLDKF 282
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,062,981
Number of Sequences: 5004
Number of extensions: 63821
Number of successful extensions: 241
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 219
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 239
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 383374054
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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