Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P09_F_G10
         (476 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc...    60   2e-10
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac...    53   2e-08
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po...    52   5e-08
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha...    48   7e-07
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c...    45   7e-06
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ...    36   0.004
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po...    34   0.013
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ...    32   0.052
SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2...    25   4.5  
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce...    25   4.5  
SPAC8C9.09c |mug129||sequence orphan|Schizosaccharomyces pombe|c...    25   4.5  
SPAC29E6.07 ||SPAC30.11|sequence orphan|Schizosaccharomyces pomb...    25   5.9  
SPBC530.02 |||membrane transporter|Schizosaccharomyces pombe|chr...    25   7.9  

>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 103

 Score = 60.1 bits (139), Expect = 2e-10
 Identities = 28/74 (37%), Positives = 40/74 (54%)
 Frame = +1

Query: 4   KLVVLAFMAPWCGPCKXIGPKLDEIAAEMXXXXXXXXXXXXXXXXXASEYNINSMPTFVF 183
           KLVV+ F A WCGPCK I PK ++  +                   A+E  +++MP+F  
Sbjct: 19  KLVVVDFFATWCGPCKAIAPKFEQF-SNTYSDATFIKVDVDQLSEIAAEAGVHAMPSFFL 77

Query: 184 VKNGKKLDEFSGAN 225
            KNG+K++E  GAN
Sbjct: 78  YKNGEKIEEIVGAN 91


>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
           Txl1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 290

 Score = 53.2 bits (122), Expect = 2e-08
 Identities = 25/67 (37%), Positives = 35/67 (52%), Gaps = 1/67 (1%)
 Frame = +1

Query: 28  APWCGPCKXIGPKLDEIAAEMXXXXXXXXXXXXXXXXX-ASEYNINSMPTFVFVKNGKKL 204
           A WCGPCK I P   ++A++                   AS   + +MPTFVF +NGK++
Sbjct: 28  ADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFENGKQI 87

Query: 205 DEFSGAN 225
           D  +GAN
Sbjct: 88  DMLTGAN 94


>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 492

 Score = 52.0 bits (119), Expect = 5e-08
 Identities = 24/73 (32%), Positives = 37/73 (50%), Gaps = 1/73 (1%)
 Frame = +1

Query: 4   KLVVLAFMAPWCGPCKXIGPKLDEIAAEM-XXXXXXXXXXXXXXXXXASEYNINSMPTFV 180
           K++++ F APWCG CK + P+ +  A E+                   SEY+I   PT  
Sbjct: 40  KVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRGYPTLN 99

Query: 181 FVKNGKKLDEFSG 219
             KNGK++ ++SG
Sbjct: 100 VFKNGKQISQYSG 112



 Score = 38.3 bits (85), Expect = 6e-04
 Identities = 20/68 (29%), Positives = 31/68 (45%)
 Frame = +1

Query: 4   KLVVLAFMAPWCGPCKXIGPKLDEIAAEMXXXXXXXXXXXXXXXXXASEYNINSMPTFVF 183
           K V++ F APWCG CK + P  +++A E                   S  +I+  PT +F
Sbjct: 374 KDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDATENDIS-VSISGFPTIMF 432

Query: 184 VKNGKKLD 207
            K   K++
Sbjct: 433 FKANDKVN 440


>SPBC12D12.07c |trx2||mitochondrial thioredoxin
           Trx2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 121

 Score = 48.0 bits (109), Expect = 7e-07
 Identities = 23/75 (30%), Positives = 38/75 (50%)
 Frame = +1

Query: 4   KLVVLAFMAPWCGPCKXIGPKLDEIAAEMXXXXXXXXXXXXXXXXXASEYNINSMPTFVF 183
           K+ V+ F A WCGPCK + P L+++ +E                  A +  + ++PT V 
Sbjct: 36  KVTVVDFYADWCGPCKYLKPFLEKL-SEQNQKASFIAVNADKFSDIAQKNGVYALPTMVL 94

Query: 184 VKNGKKLDEFSGANV 228
            + G++LD   GA+V
Sbjct: 95  FRKGQELDRIVGADV 109


>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 244

 Score = 44.8 bits (101), Expect = 7e-06
 Identities = 21/74 (28%), Positives = 34/74 (45%)
 Frame = +1

Query: 4   KLVVLAFMAPWCGPCKXIGPKLDEIAAEMXXXXXXXXXXXXXXXXXASEYNINSMPTFVF 183
           ++++L F APW  PCK +    D+ A +                  A  +++N++P FV 
Sbjct: 21  QIILLNFYAPWAAPCKQMNQVFDQFAKD-TKNAVFLKIEAEKFSDIAESFDVNAVPLFVL 79

Query: 184 VKNGKKLDEFSGAN 225
           +   K L   SGAN
Sbjct: 80  IHGAKVLARISGAN 93


>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 359

 Score = 35.5 bits (78), Expect = 0.004
 Identities = 21/76 (27%), Positives = 33/76 (43%), Gaps = 3/76 (3%)
 Frame = +1

Query: 4   KLVVLAFMAPWCGPCKXIGPKLDEIAA--EMXXXXXXXXXXXXXXXXXASEYNINSMPTF 177
           K  ++ F A WCG CK + P  +E+ A  E                  A +Y+I   PT 
Sbjct: 40  KGALIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGKIDADTHSDVADKYHITGFPTL 99

Query: 178 V-FVKNGKKLDEFSGA 222
           + F  +G +  ++S A
Sbjct: 100 IWFPPDGSEPVQYSNA 115



 Score = 28.3 bits (60), Expect = 0.64
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = +1

Query: 4   KLVVLAFMAPWCGPCKXIGPKLDEI 78
           K V++ F A WCG CK + P  + +
Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETL 183


>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 363

 Score = 33.9 bits (74), Expect = 0.013
 Identities = 18/73 (24%), Positives = 28/73 (38%), Gaps = 2/73 (2%)
 Frame = +1

Query: 13  VLAFMAPWCGPCKXIGPKLDEIAAEMXXXXXXXXXXXXXXXXXA--SEYNINSMPTFVFV 186
           ++ F APWCG CK + P   ++A+ +                 A  S+Y +   PT   V
Sbjct: 52  LVVFYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLV 111

Query: 187 KNGKKLDEFSGAN 225
               K    S  +
Sbjct: 112 YPSSKGSSLSSTD 124


>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 726

 Score = 31.9 bits (69), Expect = 0.052
 Identities = 19/68 (27%), Positives = 29/68 (42%), Gaps = 3/68 (4%)
 Frame = +1

Query: 16  LAFMAPWCGPCKXIGPKLD---EIAAEMXXXXXXXXXXXXXXXXXASEYNINSMPTFVFV 186
           + +  P CG CK +GP  D   E A E                  +S  NI ++PT    
Sbjct: 47  IKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSKELSSCANIRAVPTLYLY 106

Query: 187 KNGKKLDE 210
           +NG+ ++E
Sbjct: 107 QNGEIVEE 114


>SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis
           Mei2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 750

 Score = 25.4 bits (53), Expect = 4.5
 Identities = 12/36 (33%), Positives = 18/36 (50%)
 Frame = -2

Query: 391 NIFKFITDVKNIFSSFIKNVYMFFFF*FHLVSIGGC 284
           N   ++TD +NIF +F+ N   + F    L  I  C
Sbjct: 76  NSIDYLTDTQNIFPNFVNNENNYQFSTAPLNPIDAC 111


>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 817

 Score = 25.4 bits (53), Expect = 4.5
 Identities = 11/32 (34%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
 Frame = +3

Query: 336 FLINEEKMFLTSVMNLKILIFSVC-SLWNAKN 428
           + +N +K+  +S+  L  LIF VC  LW +++
Sbjct: 223 YQLNLDKVIHSSLKKLSFLIFKVCIRLWGSQS 254


>SPAC8C9.09c |mug129||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 302

 Score = 25.4 bits (53), Expect = 4.5
 Identities = 10/22 (45%), Positives = 16/22 (72%)
 Frame = +3

Query: 321 KNMYTFLINEEKMFLTSVMNLK 386
           +N Y +L+NE+  FLTS+  +K
Sbjct: 262 RNHYIYLLNEQVDFLTSIEIMK 283


>SPAC29E6.07 ||SPAC30.11|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 116

 Score = 25.0 bits (52), Expect = 5.9
 Identities = 12/30 (40%), Positives = 16/30 (53%)
 Frame = -2

Query: 466 FYLLNCLITTGPIFFAFQRLQTLKINIFKF 377
           FYLL+  I T P+F       T  I+I K+
Sbjct: 46  FYLLHVRINTSPLFIKTTTRPTALIHITKY 75


>SPBC530.02 |||membrane transporter|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 541

 Score = 24.6 bits (51), Expect = 7.9
 Identities = 15/43 (34%), Positives = 25/43 (58%), Gaps = 1/43 (2%)
 Frame = -2

Query: 400 LKINIFKFI-TDVKNIFSSFIKNVYMFFFF*FHLVSIGGCVQL 275
           ++I++  F+ T   +++SS I NV M F     + S+G CV L
Sbjct: 103 VQISLIAFVVTFGSSVYSSGIGNVSMDFGVSISVSSLGSCVFL 145


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,577,238
Number of Sequences: 5004
Number of extensions: 27212
Number of successful extensions: 81
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 71
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 76
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 184476110
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)