Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P09_F_A11
(800 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 60 3e-10
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 56 8e-09
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 45 1e-05
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 35 0.015
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 28 1.8
SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 27 2.3
SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II comp... 27 3.1
SPCC126.07c |||human CTD-binding SR-like protein rA9 homolog|Sch... 25 9.5
SPAC4G9.04c |||cleavage and polyadenylation specificity factor |... 25 9.5
SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac... 25 9.5
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 60.5 bits (140), Expect = 3e-10
Identities = 37/139 (26%), Positives = 52/139 (37%), Gaps = 1/139 (0%)
Frame = +1
Query: 358 YESVFANGCEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGN 537
Y C C + + +S K H CF C C +L F + K YC
Sbjct: 250 YRGNSEKSCHSCGGSLRA-GRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHL 308
Query: 538 CYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHEKCFCCVVCKNPIGTK-SFIPREQEIYC 714
Y QF+ RC C + + +HE C C I R+ +C
Sbjct: 309 DYHEQFSPRCKHCKTPIE--DQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRDDLYWC 366
Query: 715 AGCYEDKFATRCVKCNKII 771
CY++K+A +C KC K I
Sbjct: 367 QTCYDNKYAVKCKKCRKPI 385
Score = 60.5 bits (140), Expect = 3e-10
Identities = 40/165 (24%), Positives = 66/165 (40%), Gaps = 1/165 (0%)
Frame = +1
Query: 232 GEYTKAMNKDWHSGHFCCWQCDESLTGQRYVLRDEHPYCIKCYESVFANGCEECNKIIGI 411
G A K H F C C ++L + R+ YC Y F+ C+ C I
Sbjct: 268 GRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHLDYHEQFSPRCKHCK--TPI 325
Query: 412 DSKDLSYKDKHWHEACFLCAKC-RVSLVDKQFGSKLDKIYCGNCYDAQFASRCDGCGEVF 588
+ + + + +HE CA C V V+ + D +C CYD ++A +C C +
Sbjct: 326 EDQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRDDLYWCQTCYDNKYAVKCKKCRKPI 385
Query: 589 RAGTKKMEYKTRQWHEKCFCCVVCKNPIGTKSFIPREQEIYCAGC 723
+ K ++H +C+ C C +G + + E C C
Sbjct: 386 LGISVK--GSDGEYHSQCWTCGACNALLGDEGYFMIENTPICRPC 428
Score = 47.2 bits (107), Expect = 3e-06
Identities = 25/86 (29%), Positives = 35/86 (40%)
Frame = +1
Query: 541 YDAQFASRCDGCGEVFRAGTKKMEYKTRQWHEKCFCCVVCKNPIGTKSFIPREQEIYCAG 720
Y C CG RAG + + ++ H +CF C C + F RE + YC
Sbjct: 250 YRGNSEKSCHSCGGSLRAG-RIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHL 308
Query: 721 CYEDKFATRCVKCNKIITQGGVTYXN 798
Y ++F+ RC C I V N
Sbjct: 309 DYHEQFSPRCKHCKTPIEDQAVHINN 334
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 55.6 bits (128), Expect = 8e-09
Identities = 33/104 (31%), Positives = 44/104 (42%), Gaps = 3/104 (2%)
Frame = +1
Query: 262 WHSGHFCCWQCDESLTGQRYVL---RDEHPYCIKCYESVFANGCEECNKIIGIDSKDLSY 432
WH FCC +CD+ L +L D P C C A+ C C I D +S
Sbjct: 40 WHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC-----AHTCTACRMRIK-DYALMSG 93
Query: 433 KDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGNCYDAQFASR 564
D + H CF C CR ++D F I+C +C + SR
Sbjct: 94 YDSY-HRECFRCHDCRKQIIDSNFKRDNRTIFCNDCKQVRHPSR 136
Score = 44.4 bits (100), Expect = 2e-05
Identities = 30/125 (24%), Positives = 48/125 (38%), Gaps = 3/125 (2%)
Frame = +1
Query: 382 CEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSL--VDKQFGSKLD-KIYCGNCYDAQ 552
C C + +S+ WH+ CF C KC L D+ D + C +C
Sbjct: 19 CFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC---- 74
Query: 553 FASRCDGCGEVFRAGTKKMEYKTRQWHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYED 732
A C C + Y + +H +CF C C+ I +F + I+C C +
Sbjct: 75 -AHTCTACRMRIKDYALMSGYDS--YHRECFRCHDCRKQIIDSNFKRDNRTIFCNDCKQV 131
Query: 733 KFATR 747
+ +R
Sbjct: 132 RHPSR 136
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 44.8 bits (101), Expect = 1e-05
Identities = 35/145 (24%), Positives = 58/145 (40%), Gaps = 8/145 (5%)
Frame = +1
Query: 232 GEYTKAMNKDWHSGHFCCWQCDESLTGQRYVLRD-----EHPYCIKCYESVFANGCEECN 396
G+Y +A+ +H F C C+ + + + + D + P C Y C C
Sbjct: 125 GQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFRRLDLLCASCG 184
Query: 397 KIIGIDSKDLSYKDKHWHEACFLCAKC-RVSLVDKQFGSKLDKIYCGNCYDAQFASRCDG 573
+ + ++ +K +H F C+ C V + + K+YC Y FA+RC G
Sbjct: 185 --MALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYHYSTLFAARCCG 242
Query: 574 C-GEVFRAGTKKMEYKTRQ-WHEKC 642
C G + R + Q WH C
Sbjct: 243 CDGPILRQFVEVYRNGVSQNWHVPC 267
Score = 42.7 bits (96), Expect = 6e-05
Identities = 37/146 (25%), Positives = 56/146 (38%), Gaps = 7/146 (4%)
Frame = +1
Query: 382 CEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSLVDKQF---GSKLDKIY--CGNCYD 546
C C ++I + + +H CF C C + K F L+K C Y
Sbjct: 116 CASCGQVIS--GQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYF 173
Query: 547 AQFASRCDGCGEVFRAGTKKMEYKTRQWHEKCFCCVVCKNPIGTK-SFIPREQEIYCAGC 723
+ C CG R + +++H + F C +C G S+ E ++YC
Sbjct: 174 RRLDLLCASCGMALRG--YYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYH 231
Query: 724 YEDKFATRCVKCN-KIITQGGVTYXN 798
Y FA RC C+ I+ Q Y N
Sbjct: 232 YSTLFAARCCGCDGPILRQFVEVYRN 257
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 34.7 bits (76), Expect = 0.015
Identities = 34/131 (25%), Positives = 50/131 (38%), Gaps = 4/131 (3%)
Frame = +1
Query: 343 YCIKCYESVFANGCEECNKIIGIDSKDLSYKDKHWHEACFLCAKCRVSL--VDKQFGSKL 516
+CIKC+ESV + + + + K WH CF C C L + F
Sbjct: 23 FCIKCWESVPS-------------TSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDD 69
Query: 517 DK-IYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHEKCF-CCVVCKNPIGTKSFI 690
K I+C C D C+GC T E+ + CC C+ I + +F
Sbjct: 70 QKQIFCKLCVDI-----CNGC------STPICEFNAVSNSQANHPCCSNCRAFINSNAFG 118
Query: 691 PREQEIYCAGC 723
+ + YC C
Sbjct: 119 KFKGQHYCLPC 129
Score = 29.1 bits (62), Expect = 0.77
Identities = 16/58 (27%), Positives = 28/58 (48%), Gaps = 3/58 (5%)
Frame = +1
Query: 565 CDGCGEVFRAGTKKMEYKTRQWHEKCFCCVVCKNPI--GTKSFIPREQ-EIYCAGCYE 729
C C E + T ++ + + WH CF CV C + ++ F +Q +I+C C +
Sbjct: 24 CIKCWESVPS-TSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLCVD 80
Score = 27.9 bits (59), Expect = 1.8
Identities = 25/102 (24%), Positives = 40/102 (39%), Gaps = 3/102 (2%)
Frame = +1
Query: 256 KDWHSGHFCCWQCDESL--TGQRYVLRDEHP-YCIKCYESVFANGCEECNKIIGIDSKDL 426
K WHS F C C++ L + + + D+ +C C + NGC I +
Sbjct: 42 KCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLCVD--ICNGCS--TPICEFN---- 93
Query: 427 SYKDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGNCYDAQ 552
+ + + C C+ CR + FG + YC C Q
Sbjct: 94 AVSNSQANHPC--CSNCRAFINSNAFGKFKGQHYCLPCLRKQ 133
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 27.9 bits (59), Expect = 1.8
Identities = 11/29 (37%), Positives = 13/29 (44%)
Frame = +1
Query: 271 GHFCCWQCDESLTGQRYVLRDEHPYCIKC 357
GHF C +C L GQ+Y KC
Sbjct: 623 GHFTCQECMSMLVGQKYGSSSTSTIIAKC 651
>SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 131
Score = 27.5 bits (58), Expect = 2.3
Identities = 23/84 (27%), Positives = 31/84 (36%)
Frame = +1
Query: 430 YKDKHWHEACFLCAKCRVSLVDKQFGSKLDKIYCGNCYDAQFASRCDGCGEVFRAGTKKM 609
Y H C++CAKC+ L K G L Y G A R + V K
Sbjct: 4 YYPVHLKSRCYVCAKCKTHLAFK--GHLLSHDYRGKNGPACLFKRVE---NVIEMEPKTE 58
Query: 610 EYKTRQWHEKCFCCVVCKNPIGTK 681
+ T ++ + C C IG K
Sbjct: 59 QMSTGRFIVRHIHCCRCHTYIGWK 82
>SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II complex
subunit Rpb2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1210
Score = 27.1 bits (57), Expect = 3.1
Identities = 17/45 (37%), Positives = 23/45 (51%)
Frame = -1
Query: 494 STRETRHLAHKKQASCQCLSLYDRSLESMPMILLHSSQPLAKTLS 360
S R T A KQA L+ Y +++M IL + +PLA T S
Sbjct: 753 SPRNTYQSAMGKQAMGVYLTNYQVRMDTMANILYYPQKPLATTRS 797
>SPCC126.07c |||human CTD-binding SR-like protein rA9
homolog|Schizosaccharomyces pombe|chr 3|||Manual
Length = 571
Score = 25.4 bits (53), Expect = 9.5
Identities = 24/98 (24%), Positives = 44/98 (44%), Gaps = 6/98 (6%)
Frame = +1
Query: 337 HPYCIKCYESVFANGCEECN-KIIGIDSKDLSYKDKHWHEACFLCAKCR-VSLVDKQF-- 504
H +C++ + V AN C C + + +D L + W+ A + K + V+ + F
Sbjct: 61 HNHCLESWCRV-ANTCPLCRTEFLKVDV--LEFVKGPWYRAYPVEEKTQSVANAGEPFED 117
Query: 505 -GSKLDK-IYCGNCYDAQFASRCDGCGEVFRAGTKKME 612
GS+ + + CG A+ CDGC + + M+
Sbjct: 118 EGSETCRCVICGRSDHAEVLLLCDGCDDAYHTYCLNMD 155
>SPAC4G9.04c |||cleavage and polyadenylation specificity factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 638
Score = 25.4 bits (53), Expect = 9.5
Identities = 12/33 (36%), Positives = 17/33 (51%), Gaps = 2/33 (6%)
Frame = +1
Query: 358 YESVFANGCEECNKIIGIDSKDLSYKDKH--WH 450
YE+ ++N C C + G D + DKH WH
Sbjct: 416 YEN-YSNQCANCGRRYGNDPESRKELDKHSDWH 447
>SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase
E3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 905
Score = 25.4 bits (53), Expect = 9.5
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = -3
Query: 522 LVELGAELLVDQRDAALGAQEAGLVPVLVLVRQILRVNADD 400
L LG ELL + D Q A L+ V+ ++ L A+D
Sbjct: 689 LTPLGMELLQNYIDHTSDVQTAALIAAFVVPKKFLDKRAED 729
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,359,605
Number of Sequences: 5004
Number of extensions: 40408
Number of successful extensions: 150
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 388424860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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