Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P08_pT_O14
(603 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 102 5e-23
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 81 9e-17
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 48 1e-06
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 41 2e-04
SPBC29A10.08 |||1,3-beta-glucanosyltransferase |Schizosaccharomy... 30 0.23
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 27 2.1
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 27 2.8
SPCC736.11 |ago1|csp9|argonaute|Schizosaccharomyces pombe|chr 3|... 26 3.7
SPBC1709.09 |||mitochondrial translation termination factor|Schi... 26 4.9
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 26 4.9
SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 25 6.4
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac... 25 6.4
SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog... 25 6.4
SPAC27F1.03c |uch1||ubiquitin C-terminal hydrolase Uch1|Schizosa... 25 8.5
SPAC9.13c |cwf16|SPAPJ735.01c|splicing factor |Schizosaccharomyc... 25 8.5
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 102 bits (244), Expect = 5e-23
Identities = 46/142 (32%), Positives = 70/142 (49%), Gaps = 1/142 (0%)
Frame = -1
Query: 603 QELGTRNFFERDGRPYCEPDYHNLFSPRCAYCNGPILDKCVTALEKTWHTEHFFCAQCGQ 424
Q L F+ R+G+ YC DYH FSPRC +C PI D+ V +H H FCA C +
Sbjct: 290 QNLEHVGFYYREGKFYCHLDYHEQFSPRCKHCKTPIEDQAVHINNDWFHENHHFCAGCSE 349
Query: 423 QFGEE-GFHERDGKPYCRADYFDMFAPKCGGCNKPIMENYISALNTQWHPDCFVCKDCQN 247
F RD +C+ Y + +A KC C KPI+ + + ++H C+ C C
Sbjct: 350 VFNVNIPCIYRDDLYWCQTCYDNKYAVKCKKCRKPILGISVKGSDGEYHSQCWTCGACNA 409
Query: 246 PVKGKSFYAMEGKPVCPTCVGV 181
+ + ++ +E P+C C +
Sbjct: 410 LLGDEGYFMIENTPICRPCKAI 431
Score = 66.1 bits (154), Expect = 4e-12
Identities = 29/90 (32%), Positives = 43/90 (47%), Gaps = 1/90 (1%)
Frame = -1
Query: 519 CAYCNGPI-LDKCVTALEKTWHTEHFFCAQCGQQFGEEGFHERDGKPYCRADYFDMFAPK 343
C C G + + ++A K H + F C C Q GF+ R+GK YC DY + F+P+
Sbjct: 258 CHSCGGSLRAGRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHLDYHEQFSPR 317
Query: 342 CGGCNKPIMENYISALNTQWHPDCFVCKDC 253
C C PI + + N +H + C C
Sbjct: 318 CKHCKTPIEDQAVHINNDWFHENHHFCAGC 347
Score = 36.3 bits (80), Expect = 0.003
Identities = 18/48 (37%), Positives = 22/48 (45%)
Frame = -1
Query: 342 CGGCNKPIMENYISALNTQWHPDCFVCKDCQNPVKGKSFYAMEGKPVC 199
CGG + ISA + HP CF C C ++ FY EGK C
Sbjct: 261 CGGSLRA--GRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYC 306
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 81.4 bits (192), Expect = 9e-17
Identities = 38/102 (37%), Positives = 53/102 (51%), Gaps = 5/102 (4%)
Frame = -1
Query: 561 PYCEPDYHNLFSPRCAYCNGPILDKCVTALEKTWHTEHFFCAQCGQQFG-EEGFHERDGK 385
P CE DY CA C + +TAL K +H EHF C+ C FG + ++E +GK
Sbjct: 166 PLCETDYFRRLDLLCASCGMALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGK 225
Query: 384 PYCRADYFDMFAPKCGGCNKPIM----ENYISALNTQWHPDC 271
YC Y +FA +C GC+ PI+ E Y + ++ WH C
Sbjct: 226 VYCHYHYSTLFAARCCGCDGPILRQFVEVYRNGVSQNWHVPC 267
Score = 59.3 bits (137), Expect = 4e-10
Identities = 36/113 (31%), Positives = 45/113 (39%), Gaps = 6/113 (5%)
Frame = -1
Query: 519 CAYCNGPILDKCVTALEKTWHTEHFFCAQCGQQFGEEGFHERDGK-----PYCRADYFDM 355
CA C I + V AL +H E F C C + F D P C DYF
Sbjct: 116 CASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFRR 175
Query: 354 FAPKCGGCNKPIMENYISALNTQWHPDCFVCKDCQNPV-KGKSFYAMEGKPVC 199
C C + YI+ALN ++H + F C C S+Y EGK C
Sbjct: 176 LDLLCASCGMALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYC 228
Score = 41.5 bits (93), Expect = 9e-05
Identities = 17/47 (36%), Positives = 27/47 (57%), Gaps = 4/47 (8%)
Frame = -1
Query: 585 NFFERDGRPYCEPDYHNLFSPRCAYCNGPILDKCV----TALEKTWH 457
+++E +G+ YC Y LF+ RC C+GPIL + V + + WH
Sbjct: 218 SYYEYEGKVYCHYHYSTLFAARCCGCDGPILRQFVEVYRNGVSQNWH 264
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 48.0 bits (109), Expect = 1e-06
Identities = 28/103 (27%), Positives = 49/103 (47%), Gaps = 3/103 (2%)
Frame = -1
Query: 468 KTWHTEHFFCAQCGQQF--GEEGFHERDGKP-YCRADYFDMFAPKCGGCNKPIMENYISA 298
K WH++ F C C ++ E F + D K +C+ + C GC+ PI E + +
Sbjct: 42 KCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCK-----LCVDICNGCSTPICE-FNAV 95
Query: 297 LNTQWHPDCFVCKDCQNPVKGKSFYAMEGKPVCPTCVGVDDDE 169
N+Q + C C +C+ + +F +G+ C C+ D+E
Sbjct: 96 SNSQANHPC--CSNCRAFINSNAFGKFKGQHYCLPCLRKQDEE 136
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 40.7 bits (91), Expect = 2e-04
Identities = 23/94 (24%), Positives = 39/94 (41%), Gaps = 3/94 (3%)
Frame = -1
Query: 462 WHTEHFFCAQC--GQQFGEEGFHER-DGKPYCRADYFDMFAPKCGGCNKPIMENYISALN 292
WH + F C +C G + ++ + DG+P C + A C C I + + +
Sbjct: 40 WHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSS-----CAHTCTACRMRIKDYALMSGY 94
Query: 291 TQWHPDCFVCKDCQNPVKGKSFYAMEGKPVCPTC 190
+H +CF C DC+ + +F C C
Sbjct: 95 DSYHRECFRCHDCRKQIIDSNFKRDNRTIFCNDC 128
Score = 34.3 bits (75), Expect = 0.014
Identities = 15/45 (33%), Positives = 20/45 (44%), Gaps = 3/45 (6%)
Frame = -1
Query: 315 ENYISALNTQWHPDCFVCKDCQNPVKGKS---FYAMEGKPVCPTC 190
E IS WH DCF C C ++ +G+PVC +C
Sbjct: 30 ETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC 74
Score = 31.9 bits (69), Expect = 0.074
Identities = 18/65 (27%), Positives = 26/65 (40%)
Frame = -1
Query: 570 DGRPYCEPDYHNLFSPRCAYCNGPILDKCVTALEKTWHTEHFFCAQCGQQFGEEGFHERD 391
DGRP C H C C I D + + ++H E F C C +Q + F +
Sbjct: 66 DGRPVCSSCAHT-----CTACRMRIKDYALMSGYDSYHRECFRCHDCRKQIIDSNFKRDN 120
Query: 390 GKPYC 376
+C
Sbjct: 121 RTIFC 125
>SPBC29A10.08 |||1,3-beta-glucanosyltransferase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 459
Score = 30.3 bits (65), Expect = 0.23
Identities = 22/86 (25%), Positives = 38/86 (44%), Gaps = 3/86 (3%)
Frame = -2
Query: 458 TRSTSFALNAVSNLGKRVSMKGTGSPTAGLITLTCSPQNAV---VAINP*WKTISQRSTP 288
T +T+ NAV NL + +GS + +++ C N ++ +P T +
Sbjct: 368 TPNTTICSNAVKNLKCSANGTPSGSKISQVLSELCYYDNKACSSISSDPYEGTYGNYTGC 427
Query: 287 SGIQTVSFARTARIPSKGSPSMPWKG 210
+G+Q +S A A G+ S W G
Sbjct: 428 TGVQQLSIALNAYTQDHGADSCSWGG 453
>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
Srb9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1223
Score = 27.1 bits (57), Expect = 2.1
Identities = 16/40 (40%), Positives = 21/40 (52%)
Frame = -2
Query: 458 TRSTSFALNAVSNLGKRVSMKGTGSPTAGLITLTCSPQNA 339
TR T + L+ V NL K++ +PT L TLT NA
Sbjct: 215 TRQTVYRLSEVDNLVKKLDTVIRLAPTGCLATLTSVHANA 254
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 26.6 bits (56), Expect = 2.8
Identities = 12/50 (24%), Positives = 22/50 (44%)
Frame = -1
Query: 447 FFCAQCGQQFGEEGFHERDGKPYCRADYFDMFAPKCGGCNKPIMENYISA 298
++C C +F G P C +D+ +M P + P N+++A
Sbjct: 8 WYCHSCSNEFQRPG-----NCPRCNSDFVEMVEPNTAPEDDPRAANFVNA 52
>SPCC736.11 |ago1|csp9|argonaute|Schizosaccharomyces pombe|chr
3|||Manual
Length = 834
Score = 26.2 bits (55), Expect = 3.7
Identities = 10/27 (37%), Positives = 19/27 (70%)
Frame = +2
Query: 452 SVCHVFSRAVTHLSKIGPLQ*AHLGEN 532
++C+V++RA + +S + P+ AHL N
Sbjct: 766 NLCYVYARATSAVSLVPPVYYAHLVSN 792
>SPBC1709.09 |||mitochondrial translation termination
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 244
Score = 25.8 bits (54), Expect = 4.9
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = -1
Query: 537 NLFSPRCAYCNGPILDK 487
++ SPRCA+ NG IL K
Sbjct: 20 SILSPRCAFHNGGILHK 36
>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1334
Score = 25.8 bits (54), Expect = 4.9
Identities = 12/56 (21%), Positives = 25/56 (44%)
Frame = -2
Query: 242 SKGSPSMPWKGSRYAQHALESMMMNKECLRMTSPLRTNALPNFLQLCSNISKGMQT 75
+ SPS+ W S + ++ TSP+ + N L S++++ ++T
Sbjct: 144 NSNSPSLTWHTSSGDDSNQNPFFVRRQSQSSTSPVSDSVDENLLSAVSSVTESVET 199
>SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 504
Score = 25.4 bits (53), Expect = 6.4
Identities = 9/24 (37%), Positives = 12/24 (50%)
Frame = -1
Query: 489 KCVTALEKTWHTEHFFCAQCGQQF 418
KC T +EK H C +C +F
Sbjct: 293 KCSTTIEKNGGCNHMTCKKCKYEF 316
>SPCC736.14 |dis1||microtubule-associated protein Dis1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 882
Score = 25.4 bits (53), Expect = 6.4
Identities = 15/47 (31%), Positives = 24/47 (51%)
Frame = -2
Query: 491 INASQL*RKRGTRSTSFALNAVSNLGKRVSMKGTGSPTAGLITLTCS 351
+ S+L K+ ST A +A+ ++ SMK G L+T+T S
Sbjct: 643 LETSRLSTKKLPGSTMKAASALKEYPQQQSMKSGGEKQDNLVTITMS 689
>SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 982
Score = 25.4 bits (53), Expect = 6.4
Identities = 23/70 (32%), Positives = 31/70 (44%), Gaps = 4/70 (5%)
Frame = -2
Query: 443 FALNAVSNLGKRVSMKGTGSPTAGLITL-TCSPQNAVVAINP*WKTISQRST---PSGIQ 276
FA + +SNL KRV + G S G L SP N + + Q ST S +
Sbjct: 83 FAEDVLSNLAKRVEIWGANSAKTGFELLKQASPGNMQMLEDLLVSENYQESTAISDSSVS 142
Query: 275 TVSFARTARI 246
+V A T R+
Sbjct: 143 SVLLAVTTRV 152
>SPAC27F1.03c |uch1||ubiquitin C-terminal hydrolase
Uch1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 222
Score = 25.0 bits (52), Expect = 8.5
Identities = 17/73 (23%), Positives = 35/73 (47%), Gaps = 1/73 (1%)
Frame = -1
Query: 504 GPILDKCVTALEKTWHTE-HFFCAQCGQQFGEEGFHERDGKPYCRADYFDMFAPKCGGCN 328
GP L+ V E+ T+ HF C G+ + F+E DG+ + ++ +
Sbjct: 141 GPPLE--VEGSEEDVETDLHFICFVKGKSKDDNHFYELDGRQEGPVQHSEIESDLLNAEV 198
Query: 327 KPIMENYISALNT 289
+++NYI ++++
Sbjct: 199 LSVIKNYIQSIDS 211
>SPAC9.13c |cwf16|SPAPJ735.01c|splicing factor |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 25.0 bits (52), Expect = 8.5
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +3
Query: 438 RKRSAPCATFSLEL*RIYPRLVRCSKRISVRTD 536
RK FS+++ R Y R RC+ I+ TD
Sbjct: 64 RKEKTGEKYFSIDILRFYIRCTRCAAEITFITD 96
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,601,984
Number of Sequences: 5004
Number of extensions: 59969
Number of successful extensions: 157
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 264253462
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -