SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P08_pT_J07
         (388 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces pomb...    29   0.34 
SPBC31F10.14c |hip3|hir3|HIRA interacting protein Hip3|Schizosac...    27   0.78 
SPAC23C11.06c |||hydrolase |Schizosaccharomyces pombe|chr 1|||Ma...    27   1.4  
SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynei...    26   1.8  
SPBC2A9.12 |orc6|SPBC2D10.02|origin recognition complex subunit ...    25   4.1  
SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Sc...    24   7.2  
SPBC16C6.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2||...    24   7.2  
SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pomb...    24   7.2  
SPBC1289.01c |chr4|cfh3, SPBC1539.11c|chitin synthase regulatory...    24   9.6  
SPBC211.08c |||ribonuclease PH-like|Schizosaccharomyces pombe|ch...    24   9.6  
SPCPB1C11.03 |||cysteine transporter |Schizosaccharomyces pombe|...    24   9.6  

>SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 782

 Score = 28.7 bits (61), Expect = 0.34
 Identities = 16/46 (34%), Positives = 25/46 (54%)
 Frame = -2

Query: 297 INRRLLV*NTTII*NYIMPHFRIETNISRSKIPADFVVKAIPVLAK 160
           +NR L    TT   N ++PH+  + ++   K+ ADFV     +LAK
Sbjct: 612 VNRTLKF--TTAKYNGLVPHYLTDPSVGAGKLKADFVKDVNALLAK 655


>SPBC31F10.14c |hip3|hir3|HIRA interacting protein
            Hip3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1630

 Score = 27.5 bits (58), Expect = 0.78
 Identities = 16/39 (41%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
 Frame = +3

Query: 264  LLCFKREDVDLFRRNAKFTIFSMS*RLEIQYKQ--NHRP 374
            LL  K EDV++++RNA   I      + I  KQ  +HRP
Sbjct: 1315 LLEVKNEDVNIYKRNALLFILKALAEMRILDKQSWHHRP 1353


>SPAC23C11.06c |||hydrolase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 535

 Score = 26.6 bits (56), Expect = 1.4
 Identities = 19/65 (29%), Positives = 27/65 (41%), Gaps = 3/65 (4%)
 Frame = -2

Query: 243 PHF-RIETNISRSKIPADFVVKAIPV--LAKALGKPEQHVHNLPRRTYRQRWI*RNDFPR 73
           P F R E   ++S +P D  V  I      K   K       L + T R  W   N+ P 
Sbjct: 450 PSFSRYELQSAQSVLPKDLPVSVISAGKSMKRFKKWPDEQRQLSKLTQRTVWDIVNNAPH 509

Query: 72  DLWIN 58
           D+W++
Sbjct: 510 DVWLS 514


>SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynein
           Mcp5/Num1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 968

 Score = 26.2 bits (55), Expect = 1.8
 Identities = 11/23 (47%), Positives = 16/23 (69%)
 Frame = -1

Query: 274 KHNNNLKLYNASL*NRNQHFKIK 206
           K +N ++LY+  L NRN+  KIK
Sbjct: 4   KQDNEVELYSIDLKNRNESAKIK 26


>SPBC2A9.12 |orc6|SPBC2D10.02|origin recognition complex subunit
           Orc6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 264

 Score = 25.0 bits (52), Expect = 4.1
 Identities = 14/40 (35%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
 Frame = -2

Query: 231 IETNISRSKIPADFVVKA-IPVLAKALGKPEQHVHNLPRR 115
           + TN  R +     ++ A + V AKA GK E H+++  RR
Sbjct: 133 VVTNDMRIEHKRGIIIAAYLLVSAKAFGKDELHINHTERR 172


>SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1063

 Score = 24.2 bits (50), Expect = 7.2
 Identities = 10/30 (33%), Positives = 18/30 (60%)
 Frame = +1

Query: 229 YSKVRHYIILDYCCVLNEKTSIYLGEMQNL 318
           +S+    I L YC   NE+ +I L +M+++
Sbjct: 635 FSRTNQLICLFYCLTGNEEATIQLIQMRHI 664


>SPBC16C6.04 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 350

 Score = 24.2 bits (50), Expect = 7.2
 Identities = 12/37 (32%), Positives = 20/37 (54%)
 Frame = -1

Query: 280 RLKHNNNLKLYNASL*NRNQHFKIKNPS*FCGKSYTG 170
           +L +N NL   + S    N+H ++KN    C  S++G
Sbjct: 104 QLSYNENLDQRSFSK-TPNKHIEVKNLKDLCSPSHSG 139


>SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 420

 Score = 24.2 bits (50), Expect = 7.2
 Identities = 10/22 (45%), Positives = 15/22 (68%)
 Frame = +3

Query: 267 LCFKREDVDLFRRNAKFTIFSM 332
           +CFK+EDVD  ++   FT  S+
Sbjct: 13  VCFKQEDVDSLKKPNWFTDVSI 34


>SPBC1289.01c |chr4|cfh3, SPBC1539.11c|chitin synthase regulatory
           factor |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 633

 Score = 23.8 bits (49), Expect = 9.6
 Identities = 11/26 (42%), Positives = 14/26 (53%)
 Frame = -2

Query: 222 NISRSKIPADFVVKAIPVLAKALGKP 145
           NI+ S+ P   V+K IPV A     P
Sbjct: 564 NITSSQYPNQDVIKEIPVTASETSPP 589


>SPBC211.08c |||ribonuclease PH-like|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 257

 Score = 23.8 bits (49), Expect = 9.6
 Identities = 10/23 (43%), Positives = 13/23 (56%)
 Frame = -1

Query: 352 CISSLYDIEKIVNFAFLLNKSTS 284
           CI +  D   ++N A LL KS S
Sbjct: 232 CIKNAKDTRLVINHALLLEKSKS 254


>SPCPB1C11.03 |||cysteine transporter |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 570

 Score = 23.8 bits (49), Expect = 9.6
 Identities = 11/33 (33%), Positives = 17/33 (51%)
 Frame = +2

Query: 131 CTCCSGLPRALANTGIAFTTKSAGIFDLEMLVS 229
           C  C G+P      G+ F TKS   + L M+++
Sbjct: 235 CLSC-GIPAGFIAYGLEFVTKSIAPWKLFMIIT 266


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,690,923
Number of Sequences: 5004
Number of extensions: 34451
Number of successful extensions: 104
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 102
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 104
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 128344734
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -