Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P08_pT_E08
(794 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC2F7.13c |||tryptophan-tRNA ligase |Schizosaccharomyces pombe... 289 4e-79
SPCC1672.05c |||tyrosine-tRNA ligase |Schizosaccharomyces pombe|... 32 0.082
SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces po... 28 1.8
SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc... 28 1.8
SPAC821.04c |cid13||poly|Schizosaccharomyces pombe|chr 1|||Manual 27 3.1
SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 27 4.1
SPBC428.05c |arg12||argininosuccinate synthase |Schizosaccharomy... 27 4.1
SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pomb... 26 5.4
SPAC31F12.01 |zds1|SPAC637.14, mug88|zds family protein Zds1|Sch... 26 7.1
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 26 7.1
SPAC1B3.13 |||U3 snoRNP-associated protein Nan1|Schizosaccharomy... 26 7.1
SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 25 9.4
SPBC14C8.14c |pol5||DNA polymerase phi|Schizosaccharomyces pombe... 25 9.4
>SPAC2F7.13c |||tryptophan-tRNA ligase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 395
Score = 289 bits (708), Expect = 4e-79
Identities = 137/236 (58%), Positives = 178/236 (75%), Gaps = 1/236 (0%)
Frame = -1
Query: 791 GFDPSNTFIFNDLDFIGQCPAFYQNMLRIQKCVTYNQVKGIFGFGDSDVIGKITFPSIEA 612
GFDP TFIF + ++G AFYQN++RI KC+T NQ K FGF DSD IGKI F SI+A
Sbjct: 154 GFDPKKTFIFMNSTYVGG--AFYQNVVRIAKCITANQSKACFGFTDSDSIGKIHFASIQA 211
Query: 611 APAFSTTFPFIFEN-KIVHCLVPCAIDQDPYFRMTRDVAVRLKLPKPSLIHSTFLPALQG 435
AP+FS++FP IF K + CL+PCAIDQDPYFR+TRDV+ RLK KP+L+HS F PALQG
Sbjct: 212 APSFSSSFPHIFNGAKDIPCLIPCAIDQDPYFRLTRDVSGRLKFKKPALLHSRFFPALQG 271
Query: 434 AQHKMSASDCNASIFLNDTPKQIKNKINKYAFSGGQITVEEHREKGGNTDVDISYKYLVF 255
Q KMSAS +++IF+ DTP +IKNKIN++AFSGG T+E HREKGGN DVD++Y+YL F
Sbjct: 272 PQSKMSASKDSSAIFMTDTPNKIKNKINRHAFSGGGATIEIHREKGGNPDVDVAYQYLSF 331
Query: 254 FLEDDDKLAEIKKSYESGQMLTGELKKIAIDTITGYVQDYQARRAKVTDEVMHEYF 87
FL+DD+KL ++ +Y++G + TGE+K I + +V D+QA R+KV DE + F
Sbjct: 332 FLDDDEKLKQLYNTYKAGTLSTGEMKGECIKLLQQFVSDFQAARSKV-DEATLDMF 386
>SPCC1672.05c |||tyrosine-tRNA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 401
Score = 32.3 bits (70), Expect = 0.082
Identities = 20/62 (32%), Positives = 30/62 (48%)
Frame = -1
Query: 539 IDQDPYFRMTRDVAVRLKLPKPSLIHSTFLPALQGAQHKMSASDCNASIFLNDTPKQIKN 360
+DQ F M +V L K + + +P+L G + SA+ NA I + D PK +
Sbjct: 184 VDQRKIFVMAEEVLPVLGYKKRGHLMNPMVPSLAGGKMSSSAA-ANAKIDILDDPKTVNK 242
Query: 359 KI 354
KI
Sbjct: 243 KI 244
>SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 334
Score = 27.9 bits (59), Expect = 1.8
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = -1
Query: 752 DFIGQCPAFYQNMLRIQKCVTYNQVKGIFGFGDSDVIGKITFPSIE 615
DF+ +C +QN+ I C TYN K G D ++I + PS++
Sbjct: 41 DFLAKCKTEFQNVKAI--CRTYNS-KFYMGIFDKEIIDNLP-PSVK 82
>SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1284
Score = 27.9 bits (59), Expect = 1.8
Identities = 21/59 (35%), Positives = 30/59 (50%), Gaps = 1/59 (1%)
Frame = +3
Query: 258 N*ILVRNIHISVTTLLSVLLHCDLTSRKRIFVYLI-FYLLRSVVQEYRSVTITSRHFVL 431
N I RN+ +V L SVL +C + S LI FY ++ + VT + RHF+L
Sbjct: 663 NSIYARNLFNTVEQLQSVLPNCHVPSNLSTESLLIKFYTNQNKIS--ADVTASDRHFLL 719
>SPAC821.04c |cid13||poly|Schizosaccharomyces pombe|chr 1|||Manual
Length = 578
Score = 27.1 bits (57), Expect = 3.1
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = +3
Query: 627 ECNLANDIRITETEYALYLVVCDTFLYP*HVLVKSWA 737
+CN+ I T V+CD + P V++K WA
Sbjct: 164 DCNINKTISTLNTRLMRSYVLCDPRVRPLIVMIKYWA 200
>SPBC29A10.10c |||tRNA-splicing endonuclease positive effector
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1944
Score = 26.6 bits (56), Expect = 4.1
Identities = 11/15 (73%), Positives = 13/15 (86%)
Frame = +1
Query: 379 VSFKNIEALQSLADI 423
V FKN++ LQSLADI
Sbjct: 386 VGFKNVQLLQSLADI 400
>SPBC428.05c |arg12||argininosuccinate synthase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 410
Score = 26.6 bits (56), Expect = 4.1
Identities = 19/79 (24%), Positives = 32/79 (40%), Gaps = 3/79 (3%)
Frame = -1
Query: 635 ITFPSIEAAPAFSTTF-PFIFENKIVHCLVPCAIDQDPYFRMTRDVAVRLKLPK--PSLI 465
+ + + + P TT P+ + IVHC I +DP +D+ PK P +
Sbjct: 162 LEYAAAKGIPVTQTTKKPWSMDENIVHCSYEAGILEDPSMTPPKDMWKLTVDPKDAPDEV 221
Query: 464 HSTFLPALQGAQHKMSASD 408
+ +GA K+ D
Sbjct: 222 EELSIHFEKGAPTKLECKD 240
>SPCC895.08c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 490
Score = 26.2 bits (55), Expect = 5.4
Identities = 11/20 (55%), Positives = 11/20 (55%)
Frame = +2
Query: 275 KYPHQCYHPSLCAPPL*FDL 334
KYP QCY C PL DL
Sbjct: 179 KYPQQCYKGPGCVCPLLIDL 198
>SPAC31F12.01 |zds1|SPAC637.14, mug88|zds family protein
Zds1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 938
Score = 25.8 bits (54), Expect = 7.1
Identities = 13/44 (29%), Positives = 21/44 (47%)
Frame = -1
Query: 479 KPSLIHSTFLPALQGAQHKMSASDCNASIFLNDTPKQIKNKINK 348
KP + H +P + +K S ++ S + TPKQ + NK
Sbjct: 625 KPEIFHERHIPTPETGPNKPSKNNILKSTQVPVTPKQKSSTANK 668
>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1957
Score = 25.8 bits (54), Expect = 7.1
Identities = 11/39 (28%), Positives = 22/39 (56%)
Frame = -1
Query: 185 ELKKIAIDTITGYVQDYQARRAKVTDEVMHEYFRIRKID 69
++K ++++ + QAR A++ DEV +IR+ D
Sbjct: 1663 KIKSNTVESLQDVLTSVQARNAELEDEVSRSVDKIRRRD 1701
>SPAC1B3.13 |||U3 snoRNP-associated protein Nan1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 800
Score = 25.8 bits (54), Expect = 7.1
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = -1
Query: 746 IGQCPAFYQNMLRIQKCVTYNQVKGIFGFGDSDVIGKITFPS 621
+ + PA Y N + +TY+ GIF D I +I FP+
Sbjct: 27 VSKIPAVYSNDNKFV-FLTYDTFIGIFSLITGDCINRIFFPN 67
>SPBC4F6.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 674
Score = 25.4 bits (53), Expect = 9.4
Identities = 19/75 (25%), Positives = 30/75 (40%)
Frame = -1
Query: 509 RDVAVRLKLPKPSLIHSTFLPALQGAQHKMSASDCNASIFLNDTPKQIKNKINKYAFSGG 330
R +AV + K + +T A ++K SD SI D +++ S G
Sbjct: 176 RPIAVDWAVSKDAFEATTLKDASSEEENKEFVSDEGHSIVTEDASADSESEEEVDGHSEG 235
Query: 329 QITVEEHREKGGNTD 285
+ E E+G N D
Sbjct: 236 KELAGESEEEGSNVD 250
>SPBC14C8.14c |pol5||DNA polymerase phi|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 959
Score = 25.4 bits (53), Expect = 9.4
Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 4/44 (9%)
Frame = -1
Query: 581 IFENKIVHCLVPCAIDQDPY-FRMTRDVAVRL-KLPK--PSLIH 462
IF +HCL+ D+D Y +R + V +L K+ K P+L++
Sbjct: 315 IFSKNFLHCLINHLSDEDRYLYRAAKRVTSKLEKVSKQNPTLVY 358
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,314,934
Number of Sequences: 5004
Number of extensions: 70176
Number of successful extensions: 202
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 191
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 387388442
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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