Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P08_pT_D03
(804 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom... 288 5e-79
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 31 0.19
SPBC16A3.13 |meu7|aah4|alpha-amylase homolog Aah4|Schizosaccharo... 29 1.0
SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom... 27 3.1
SPAC926.02 |||conserved fungal protein|Schizosaccharomyces pombe... 26 5.5
SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 25 9.5
>SPAC6G10.08 |idp1||isocitrate dehydrogenase
Idp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 418
Score = 288 bits (707), Expect = 5e-79
Identities = 134/210 (63%), Positives = 158/210 (75%)
Frame = -2
Query: 803 SFKFALDXKYPLYLSTKNTILKKYDGRFKDIFQDIYDREYKXQXEDAGIWYEHRLIDDMV 624
SF+ AL K PLYLSTKNTILKKYDGRFKD FQ++Y+ +YK + E+ G+WY+HRLIDDMV
Sbjct: 206 SFQMALQKKMPLYLSTKNTILKKYDGRFKDTFQEVYESDYKQKFEELGLWYQHRLIDDMV 265
Query: 623 AYAMKSEGGFVWACXNYDGDVQSDSVAQGYGSLGLXTSVLICPDGKTVEAEAAHGTVTRH 444
A A+KS GGFVWAC NYDGDV SD VAQ YGSLGL TSVLI P+G+T E+EAAHGTV RH
Sbjct: 266 AQAIKSNGGFVWACKNYDGDVMSDVVAQAYGSLGLMTSVLIHPNGRTFESEAAHGTVQRH 325
Query: 443 FRFYQQGKETSTNPIASIFAWTRGLLHRAKLDNNDALXNFAETLEKVCIETIESGIMTKD 264
+ Y +GK+TSTN IASIFAWTRGL HR +LD N+ L FA LE C+ +E GIMTKD
Sbjct: 326 YMQYLKGKKTSTNSIASIFAWTRGLAHRGRLDGNERLVKFANALEHACVRCVEKGIMTKD 385
Query: 263 LAICIKGMNNVKRSDYYETFEFMDKLAENL 174
L + K N Y +TFEF+D + L
Sbjct: 386 LYLLSKSPNG-----YVDTFEFLDAVKSEL 410
>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 362
Score = 31.1 bits (67), Expect = 0.19
Identities = 23/71 (32%), Positives = 32/71 (45%), Gaps = 1/71 (1%)
Frame = -2
Query: 665 AGIWYEHRLIDDMVAYAMKSEGGF-VWACXNYDGDVQSDSVAQGYGSLGLXTSVLICPDG 489
A I + +++D MV + F V N GD+ SD A GSLGL S +
Sbjct: 222 ASINVDEQIVDSMVYRLFREPECFDVVVAPNLYGDILSDGAASLIGSLGLVPSANV--GD 279
Query: 488 KTVEAEAAHGT 456
V +E HG+
Sbjct: 280 NFVMSEPVHGS 290
>SPBC16A3.13 |meu7|aah4|alpha-amylase homolog
Aah4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 774
Score = 28.7 bits (61), Expect = 1.0
Identities = 19/57 (33%), Positives = 25/57 (43%), Gaps = 1/57 (1%)
Frame = +2
Query: 533 HSPEQHYQIVRLHHNFXRPRQIHPLIS*HKPPYHQLACVHTRYQHPXTVSCT-PCHK 700
H P +H + H F RP + HKP H+ C H R+ P + T P HK
Sbjct: 278 HKPWKHEEHCH-HGKFPRPIPHNGTKPDHKPWKHEEHCHHGRFPRPVPHNGTKPDHK 333
Score = 27.5 bits (58), Expect = 2.4
Identities = 18/57 (31%), Positives = 25/57 (43%), Gaps = 1/57 (1%)
Frame = +2
Query: 533 HSPEQHYQIVRLHHNFXRPRQIHPLIS*HKPPYHQLACVHTRYQHPXTVSCT-PCHK 700
H P +H + H F RP + HKP H+ C H ++ P + T P HK
Sbjct: 361 HKPWKHEEHCH-HGKFLRPVPHNVTKPDHKPWKHEEHCHHGKFPRPVPHNGTKPDHK 416
Score = 25.8 bits (54), Expect = 7.2
Identities = 18/59 (30%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = +2
Query: 530 THSPEQHYQIVRLHHN-FXRPRQIHPLIS*HKPPYHQLACVHTRYQHPXTVSCT-PCHK 700
TH + + HH+ F RP + HKP H+ C H ++ P + T P HK
Sbjct: 163 THIDHKCKEHCSCHHDKFPRPVPHNGTKPDHKPWKHEEHCHHGKFPRPVPHNGTKPDHK 221
>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 27.1 bits (57), Expect = 3.1
Identities = 51/185 (27%), Positives = 76/185 (41%), Gaps = 6/185 (3%)
Frame = -2
Query: 755 KNTILKKYDGRF----KDIFQDIYDREYKXQXEDAGIWYEHRLIDDMVAYAMKSEGGFVW 588
K TI++ DG F K++ + D E + + D +++ D V Y V
Sbjct: 213 KATIMRMADGLFLECAKELAPEYPDIELREEILDNACL---KIVTDPVPY-----NNTVM 264
Query: 587 ACXNYDGDVQSDSVAQGYGSLGLXTSVLICPDGKTVEAEAAHGTVTRHFRFYQQGKETST 408
N GD+ SD A G LGL S I + EA HGT GK +
Sbjct: 265 VMPNLYGDIVSDMCAGLIGGLGLTPSGNI--GNQASIFEAVHGTAPD-----IAGKGLA- 316
Query: 407 NPIASIFAWTRGLLHRAKLDNNDALXNFAETLEKVCIETIESG--IMTKDLAICIKGMNN 234
NP A + + L H ++ ND +A+ +E +T+ + TKDL NN
Sbjct: 317 NPTALLLSSVMMLKH---MNLND----YAKRIESAIFDTLANNPDARTKDLG---GKSNN 366
Query: 233 VKRSD 219
V+ +D
Sbjct: 367 VQYTD 371
>SPAC926.02 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 443
Score = 26.2 bits (55), Expect = 5.5
Identities = 15/31 (48%), Positives = 18/31 (58%), Gaps = 3/31 (9%)
Frame = -2
Query: 350 DNNDALXNFAETLEKVCIETIESG---IMTK 267
D DA +FAETL C+ T ES IMT+
Sbjct: 301 DKADAYIDFAETLLDSCVSTEESASIEIMTR 331
>SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase
Cdc25|Schizosaccharomyces pombe|chr 1|||Manual
Length = 596
Score = 25.4 bits (53), Expect = 9.5
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = -2
Query: 758 TKNTILKKYDGRFKDIF 708
T+ T+L DG+FKDIF
Sbjct: 414 TQETLLGLLDGKFKDIF 430
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,038,830
Number of Sequences: 5004
Number of extensions: 57743
Number of successful extensions: 132
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 116
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 126
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 390427050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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