Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P08_pT_B04
(764 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 298 5e-82
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 298 5e-82
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 298 5e-82
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 95 1e-20
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 54 3e-08
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 44 3e-05
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.9
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 26 6.8
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos... 25 9.0
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 298 bits (732), Expect = 5e-82
Identities = 136/180 (75%), Positives = 155/180 (86%)
Frame = -3
Query: 762 VPVGRVEXGVLKPGTIVVFAPANIXXEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELR 583
VPVGRVE GV+KPG IV FAPA + EVKSVEMHHE+L +PGDNVGFNVKNVSVK++R
Sbjct: 260 VPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIR 319
Query: 582 RGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDR 403
RG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DR
Sbjct: 320 RGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDR 379
Query: 402 RTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 223
R+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 380 RSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 298 bits (732), Expect = 5e-82
Identities = 136/180 (75%), Positives = 155/180 (86%)
Frame = -3
Query: 762 VPVGRVEXGVLKPGTIVVFAPANIXXEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELR 583
VPVGRVE GV+KPG IV FAPA + EVKSVEMHHE+L +PGDNVGFNVKNVSVK++R
Sbjct: 260 VPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIR 319
Query: 582 RGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDR 403
RG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DR
Sbjct: 320 RGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDR 379
Query: 402 RTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 223
R+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 380 RSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 298 bits (732), Expect = 5e-82
Identities = 136/180 (75%), Positives = 155/180 (86%)
Frame = -3
Query: 762 VPVGRVEXGVLKPGTIVVFAPANIXXEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELR 583
VPVGRVE GV+KPG IV FAPA + EVKSVEMHHE+L +PGDNVGFNVKNVSVK++R
Sbjct: 260 VPVGRVETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIR 319
Query: 582 RGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDR 403
RG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLDCHTAHIACKFAE+ EK+DR
Sbjct: 320 RGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDR 379
Query: 402 RTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 223
R+GK E +PK +KSGDA I +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 380 RSGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 94.7 bits (225), Expect = 1e-20
Identities = 56/180 (31%), Positives = 95/180 (52%), Gaps = 2/180 (1%)
Frame = -3
Query: 753 GRVEXGVLKPGTIVVFAPANIXXEVKSV-EMHHEALQEAVPGDNVGFNVKNVSVKELRRG 577
G++E G +K + V+ P N EV ++ + E + ++ GD V V+ +++ G
Sbjct: 486 GKIEAGSIKKNSNVLVMPINQTLEVTAIYDEADEEISSSICGDQVRLRVRGDD-SDVQTG 544
Query: 576 YVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRT 397
YV +KN P F AQ+ +L P ++ GY+ V+ HTA FA++ K+D+ T
Sbjct: 545 YVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVMHIHTAVEEVSFAKLLHKLDK-T 602
Query: 396 GKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 220
+ ++ P G I L P+C+E F+++ +GRF +RD TVAVG V+K ++
Sbjct: 603 NRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 53.6 bits (123), Expect = 3e-08
Identities = 49/176 (27%), Positives = 79/176 (44%), Gaps = 1/176 (0%)
Frame = -3
Query: 753 GRVEXGVLKPGTIVVFAPANIXXEVKSVEMHHEALQE-AVPGDNVGFNVKNVSVKELRRG 577
GRVE G ++ ++ + VK+V + + AV GD V + ++ V +LR G
Sbjct: 423 GRVEAGNVQVNQVLYDVSSQEDAYVKNVIRNSDPSSTWAVAGDTVTLQLADIEVNQLRPG 482
Query: 576 YVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKVDRRT 397
+ + +N P + F A++ + G I +G T VL H+ + K+
Sbjct: 483 DILSNYEN-PVRRVRSFVAEIQTFDIHGPILSGSTLVL-----HLGRTVTSVSLKIVTVN 536
Query: 396 GKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 229
K + + S K I L PLC+ +E P LGRF +R TVA G++K
Sbjct: 537 NKRSR-HIASRKRALVRISFLDGLFPLCLA--EECPALGRFILRRSGDTVAAGIVK 589
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 43.6 bits (98), Expect = 3e-05
Identities = 42/179 (23%), Positives = 75/179 (41%), Gaps = 2/179 (1%)
Frame = -3
Query: 762 VPVGRVEXGVLKPGTIV--VFAPANIXXEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKE 589
V GRVE G LK G + V +++ V +EM + L AV GDN G ++++ ++
Sbjct: 269 VVTGRVERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQ 328
Query: 588 LRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFAEIKEKV 409
L+RG + P F A +L + T +D + + + +++ ++
Sbjct: 329 LKRGMIVAQPGTVAPH--QKFKASFYILTK--EEGGRRTGFVDKYRPQLYSRTSDVTVEL 384
Query: 408 DRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVI 232
T + K + GD + P+ +E Q RF VR+ TV ++
Sbjct: 385 ---THPDPNDSDKMVMPGDNVEMICTLIHPIVIEKGQ------RFTVREGGSTVGTALV 434
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.9
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 586 QFLDGHVLYVETYIVSRYSFLESFV 660
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 670
Score = 25.8 bits (54), Expect = 6.8
Identities = 24/85 (28%), Positives = 42/85 (49%), Gaps = 2/85 (2%)
Frame = +2
Query: 179 SAALVTLPPPASLKLTALMTPTATVCLMSRTAKRPRGGNSWKDSTH--RGLEGTKLTMAA 352
S +VTLPPPAS ++ T T T + S ++ G+ + +++ + ++++
Sbjct: 183 STDIVTLPPPAS-STSSFSTITNTSMIPSSSSFTTTTGSPYYNTSSFLPSSVISSASLSS 241
Query: 353 SPDLMDFGLTSVDLPVRRSTFSLIS 427
S L +TS PV S+ SL S
Sbjct: 242 SSVLPTSIITSTSTPVTVSSSSLSS 266
>SPAC631.01c |acp2||F-actin capping protein beta subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 268
Score = 25.4 bits (53), Expect = 9.0
Identities = 11/25 (44%), Positives = 18/25 (72%)
Frame = +2
Query: 344 MAASPDLMDFGLTSVDLPVRRSTFS 418
++ +PDL D L+SVD P++ +T S
Sbjct: 27 LSVAPDLADVLLSSVDQPLKVNTCS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,097,050
Number of Sequences: 5004
Number of extensions: 62828
Number of successful extensions: 175
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 169
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 367316502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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