Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P08_F_P19
(768 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein... 69 1e-12
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual 67 2e-12
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 31 0.14
SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr 1||... 31 0.24
SPBC3D6.12 |||U3 snoRNA associted protein Dip2 |Schizosaccharomy... 26 5.2
SPAC823.12 |||zinc finger protein Pep5/Vps11 |Schizosaccharomyce... 26 5.2
SPBC17F3.01c |rga5|SPBC557.01|GTPase activating protein Rga5|Sch... 26 6.8
>SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein
Stg1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 174
Score = 68.5 bits (160), Expect = 1e-12
Identities = 50/162 (30%), Positives = 80/162 (49%), Gaps = 2/162 (1%)
Frame = +2
Query: 140 EKEAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENI 319
EKEA+EWIE L K D L+ G +LC++ + ++ + + F MENI
Sbjct: 6 EKEAREWIEETLHTKLNAQLDLLDQLQSGVILCRICKEALGANI-RYKESNMPFVQMENI 64
Query: 320 TNF-QSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFALGRETYRHAEWSGPCLGPKPA 496
+ F A + VP D+FQT DL+E+++ QV+ ++ + R + LGPK A
Sbjct: 65 SAFINYAQQVVHVPSQDMFQTSDLFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKLA 124
Query: 497 DECKRDFS-DEVLKAGQTVIGLQAGSNKGATQSGQNLGAGRK 619
++ R FS + + + V LQ GS TQ + + R+
Sbjct: 125 EKKPRVFSAQQQREFREGVNSLQYGSFDMPTQGTEKIAFSRR 166
>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1489
Score = 67.3 bits (157), Expect = 2e-12
Identities = 35/98 (35%), Positives = 55/98 (56%), Gaps = 1/98 (1%)
Frame = +2
Query: 146 EAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGG-QFKMMENIT 322
EA++WIE LG P FE L++G VL L+ K +P + KI + QF+ +NI
Sbjct: 46 EAKKWIEECLGTDLGPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNIN 105
Query: 323 NFQSAIKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 436
F I G+P+I F+ D++E K++ +V+ + AL
Sbjct: 106 KFLDFIHGIGLPEIFHFELTDIYEGKNLPKVIYCIHAL 143
>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
2|||Manual
Length = 962
Score = 31.5 bits (68), Expect = 0.14
Identities = 28/101 (27%), Positives = 49/101 (48%), Gaps = 4/101 (3%)
Frame = +2
Query: 146 EAQEWIEGVLGAKFPPGELFEDVLKDGTVLCQLINKLKPGSVPKINTTGGQFKMMENITN 325
EA++W+E ++ + F D L +G VLCQL K P + ++++ E T
Sbjct: 69 EAKKWLEEETNNEYQNLDDFVDALVNGKVLCQLAFKYYP---KLASNWKPRYQISERNTV 125
Query: 326 FQSA----IKAYGVPDIDVFQTVDLWEKKDIAQVVSTLFAL 436
+ +A + G+ F+T DL + +I +V+ L AL
Sbjct: 126 YLNAFFHFLDFIGMFTPFRFETKDLVRRFNIPKVIYCLHAL 166
>SPAC4F10.13c |mpd2||GYF domain|Schizosaccharomyces pombe|chr
1|||Manual
Length = 992
Score = 30.7 bits (66), Expect = 0.24
Identities = 23/64 (35%), Positives = 33/64 (51%), Gaps = 7/64 (10%)
Frame = -1
Query: 264 PGFSLLMSWQR----TVPSLRTSSNSSPGG--NLA-PRTPSIHSWASFSFYRVALGSDLS 106
PG L QR T P L TSS++ PGG N+A T S+ +S +++ + +S
Sbjct: 210 PGVGTLQQPQRAGSDTFPDLNTSSSNQPGGEPNVASANTHSLEILSSSAYHPSGSSNGIS 269
Query: 105 ANLT 94
A LT
Sbjct: 270 AGLT 273
>SPBC3D6.12 |||U3 snoRNA associted protein Dip2 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 922
Score = 26.2 bits (55), Expect = 5.2
Identities = 22/72 (30%), Positives = 33/72 (45%), Gaps = 7/72 (9%)
Frame = +2
Query: 65 SHNHTTCLWNVKFALRSLPS-----ATL*KEKEAQ-EWIEGVLGAKF-PPGELFEDVLKD 223
S +HT LW++K + +P TL E+ Q ++ + VL K P G L D
Sbjct: 496 SADHTVKLWSLKSSFDFVPGTTRKVTTLKLEQTRQIDFTDDVLAVKISPDGRFVAASLLD 555
Query: 224 GTVLCQLINKLK 259
TV ++ LK
Sbjct: 556 NTVKVYYLDSLK 567
>SPAC823.12 |||zinc finger protein Pep5/Vps11 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 906
Score = 26.2 bits (55), Expect = 5.2
Identities = 13/53 (24%), Positives = 26/53 (49%)
Frame = +3
Query: 36 RIISTLGKSTHTITQHVSGTSSSR*DRFQAQPCRRRRKPKSGLRASSELNSPL 194
+++ LGKS + +H+S + S D ++ ++ + G R E+ S L
Sbjct: 804 QLLDILGKSVNIKLEHISDSMQSVLDNYRESISKQNEAIEMGKRDIEEITSQL 856
>SPBC17F3.01c |rga5|SPBC557.01|GTPase activating protein
Rga5|Schizosaccharomyces pombe|chr 2|||Manual
Length = 361
Score = 25.8 bits (54), Expect = 6.8
Identities = 20/67 (29%), Positives = 30/67 (44%)
Frame = -1
Query: 312 SIILNCPPVVLILGTEPGFSLLMSWQRTVPSLRTSSNSSPGGNLAPRTPSIHSWASFSFY 133
+I L+ P ++ T P S + Q P+ +S S + PR SI W SFS +
Sbjct: 247 TIPLSSLPPLVAAPTSPNTSSTLITQSAAPT-PIASTSIRINSFNPRNGSIKRWRSFSRH 305
Query: 132 RVALGSD 112
A S+
Sbjct: 306 SSATYSN 312
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,236,955
Number of Sequences: 5004
Number of extensions: 71236
Number of successful extensions: 184
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 184
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 369323696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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