Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P08_F_O14
(833 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 126 4e-30
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 103 2e-23
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 47 3e-06
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 42 1e-04
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 27 3.3
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 27 4.3
SPCC736.11 |ago1|csp9|argonaute|Schizosaccharomyces pombe|chr 3|... 26 5.7
SPBC1709.09 |||mitochondrial translation termination factor|Schi... 26 7.6
SPAC56F8.08 |mud1|ucp1, ddi1|UBA domain protein Mud1|Schizosacch... 26 7.6
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 126 bits (304), Expect = 4e-30
Identities = 57/174 (32%), Positives = 90/174 (51%), Gaps = 2/174 (1%)
Frame = +3
Query: 231 CNACEKPI-VGQVITALGRTWHPEHFTCAHCNQELGTRNFFERDGRPYCEPDYHNLFSPR 407
C++C + G++I+A G+ HP+ F C C+Q L F+ R+G+ YC DYH FSPR
Sbjct: 258 CHSCGGSLRAGRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHLDYHEQFSPR 317
Query: 408 CAYCNGPILDKCVTALEKTWHTEHFFCAQCGQQFGEE-GFHERDGKPYCRADYFDMFAPK 584
C +C PI D+ V +H H FCA C + F RD +C+ Y + +A K
Sbjct: 318 CKHCKTPIEDQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRDDLYWCQTCYDNKYAVK 377
Query: 585 CGGCNKPIMENYISALNTQWHPXCFVCKDCXNPVKGKSFYAMEGKPVCPTCVGV 746
C C KPI+ + + ++H C+ C C + + ++ +E P+C C +
Sbjct: 378 CKKCRKPILGISVKGSDGEYHSQCWTCGACNALLGDEGYFMIENTPICRPCKAI 431
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 103 bits (248), Expect = 2e-23
Identities = 55/153 (35%), Positives = 77/153 (50%), Gaps = 11/153 (7%)
Frame = +3
Query: 231 CNACEKPIVGQVITALGRTWHPEHFTCAHCNQELGTRNFFERDGR------PYCEPDYHN 392
C +C + I GQ + ALG +H E F C CN + ++ FF D P CE DY
Sbjct: 116 CASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASK-FFPIDDPTLNKQVPLCETDYFR 174
Query: 393 LFSPRCAYCNGPILDKCVTALEKTWHTEHFFCAQCGQQFG-EEGFHERDGKPYCRADYFD 569
CA C + +TAL K +H EHF C+ C FG + ++E +GK YC Y
Sbjct: 175 RLDLLCASCGMALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYHYST 234
Query: 570 MFAPKCGGCNKPIM----ENYISALNTQWHPXC 656
+FA +C GC+ PI+ E Y + ++ WH C
Sbjct: 235 LFAARCCGCDGPILRQFVEVYRNGVSQNWHVPC 267
Score = 58.0 bits (134), Expect = 2e-09
Identities = 36/113 (31%), Positives = 44/113 (38%), Gaps = 6/113 (5%)
Frame = +3
Query: 408 CAYCNGPILDKCVTALEKTWHTEHFFCAQCGQQFGEEGFHERDGK-----PYCRADYFDM 572
CA C I + V AL +H E F C C + F D P C DYF
Sbjct: 116 CASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFRR 175
Query: 573 FAPKCGGCNKPIMENYISALNTQWHPXCFVCKDCXNPV-KGKSFYAMEGKPVC 728
C C + YI+ALN ++H F C C S+Y EGK C
Sbjct: 176 LDLLCASCGMALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYC 228
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 47.2 bits (107), Expect = 3e-06
Identities = 28/103 (27%), Positives = 48/103 (46%), Gaps = 3/103 (2%)
Frame = +3
Query: 459 KTWHTEHFFCAQCGQQF--GEEGFHERDGKP-YCRADYFDMFAPKCGGCNKPIMENYISA 629
K WH++ F C C ++ E F + D K +C+ + C GC+ PI E + +
Sbjct: 42 KCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCK-----LCVDICNGCSTPICE-FNAV 95
Query: 630 LNTQWHPXCFVCKDCXNPVKGKSFYAMEGKPVCPTCVGVDDDE 758
N+Q + C C +C + +F +G+ C C+ D+E
Sbjct: 96 SNSQANHPC--CSNCRAFINSNAFGKFKGQHYCLPCLRKQDEE 136
Score = 43.2 bits (97), Expect = 5e-05
Identities = 31/125 (24%), Positives = 49/125 (39%), Gaps = 5/125 (4%)
Frame = +3
Query: 192 MSRQGVQTP-QKGC-CNACEK--PIVGQVITALGRTWHPEHFTCAHCNQELGTRNFFERD 359
+ R ++TP ++ C C C + P QV G+ WH + F C +CN++L +
Sbjct: 9 LDRLSLETPSERTCFCIKCWESVPSTSQVWFG-GKCWHSDCFKCVNCNKKL------DPS 61
Query: 360 GRPYCEPDYHNLFSPRCA-YCNGPILDKCVTALEKTWHTEHFFCAQCGQQFGEEGFHERD 536
+ + D +F C CNG C H C+ C F +
Sbjct: 62 SEDFSQDDQKQIFCKLCVDICNGCSTPICEFNAVSNSQANHPCCSNCRAFINSNAFGKFK 121
Query: 537 GKPYC 551
G+ YC
Sbjct: 122 GQHYC 126
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 41.5 bits (93), Expect = 1e-04
Identities = 25/94 (26%), Positives = 37/94 (39%), Gaps = 3/94 (3%)
Frame = +3
Query: 279 GRTWHPEHFTCAHCNQELGTRNFF---ERDGRPYCEPDYHNLFSPRCAYCNGPILDKCVT 449
G WH + F C C++ L + DGRP C H C C I D +
Sbjct: 37 GHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSCAHT-----CTACRMRIKDYALM 91
Query: 450 ALEKTWHTEHFFCAQCGQQFGEEGFHERDGKPYC 551
+ ++H E F C C +Q + F + +C
Sbjct: 92 SGYDSYHRECFRCHDCRKQIIDSNFKRDNRTIFC 125
Score = 38.7 bits (86), Expect = 0.001
Identities = 23/94 (24%), Positives = 37/94 (39%), Gaps = 3/94 (3%)
Frame = +3
Query: 465 WHTEHFFCAQC--GQQFGEEGFHER-DGKPYCRADYFDMFAPKCGGCNKPIMENYISALN 635
WH + F C +C G + ++ + DG+P C + A C C I + + +
Sbjct: 40 WHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSS-----CAHTCTACRMRIKDYALMSGY 94
Query: 636 TQWHPXCFVCKDCXNPVKGKSFYAMEGKPVCPTC 737
+H CF C DC + +F C C
Sbjct: 95 DSYHRECFRCHDCRKQIIDSNFKRDNRTIFCNDC 128
Score = 31.1 bits (67), Expect = 0.20
Identities = 14/45 (31%), Positives = 19/45 (42%), Gaps = 3/45 (6%)
Frame = +3
Query: 612 ENYISALNTQWHPXCFVCKDCXNPVKGKS---FYAMEGKPVCPTC 737
E IS WH CF C C ++ +G+PVC +C
Sbjct: 30 ETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSSC 74
Score = 28.3 bits (60), Expect = 1.4
Identities = 14/45 (31%), Positives = 22/45 (48%)
Frame = +3
Query: 231 CNACEKPIVGQVITALGRTWHPEHFTCAHCNQELGTRNFFERDGR 365
C AC I + + ++H E F C C +++ N F+RD R
Sbjct: 78 CTACRMRIKDYALMSGYDSYHRECFRCHDCRKQIIDSN-FKRDNR 121
>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
Srb9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1223
Score = 27.1 bits (57), Expect = 3.3
Identities = 16/40 (40%), Positives = 21/40 (52%)
Frame = +1
Query: 469 TRSTSFALNAVSNLGKRVSMKGTGSPTAGLITLTCSPQNA 588
TR T + L+ V NL K++ +PT L TLT NA
Sbjct: 215 TRQTVYRLSEVDNLVKKLDTVIRLAPTGCLATLTSVHANA 254
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 26.6 bits (56), Expect = 4.3
Identities = 12/50 (24%), Positives = 22/50 (44%)
Frame = +3
Query: 480 FFCAQCGQQFGEEGFHERDGKPYCRADYFDMFAPKCGGCNKPIMENYISA 629
++C C +F G P C +D+ +M P + P N+++A
Sbjct: 8 WYCHSCSNEFQRPG-----NCPRCNSDFVEMVEPNTAPEDDPRAANFVNA 52
>SPCC736.11 |ago1|csp9|argonaute|Schizosaccharomyces pombe|chr
3|||Manual
Length = 834
Score = 26.2 bits (55), Expect = 5.7
Identities = 10/27 (37%), Positives = 19/27 (70%)
Frame = -2
Query: 475 SVCHVFSRAVTHLSKIGPLQ*AHLGEN 395
++C+V++RA + +S + P+ AHL N
Sbjct: 766 NLCYVYARATSAVSLVPPVYYAHLVSN 792
>SPBC1709.09 |||mitochondrial translation termination
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 244
Score = 25.8 bits (54), Expect = 7.6
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = +3
Query: 390 NLFSPRCAYCNGPILDK 440
++ SPRCA+ NG IL K
Sbjct: 20 SILSPRCAFHNGGILHK 36
>SPAC56F8.08 |mud1|ucp1, ddi1|UBA domain protein
Mud1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 332
Score = 25.8 bits (54), Expect = 7.6
Identities = 10/39 (25%), Positives = 19/39 (48%)
Frame = +2
Query: 104 TRVAGTVPHQPASTRVRVSRAYARISSGRHEPPRSTNPP 220
++ + + P+ + TR+ + A + G PP NPP
Sbjct: 253 SKASASSPNPQSGTRLGTKESVAPNNEGSSNPPSLVNPP 291
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,597,147
Number of Sequences: 5004
Number of extensions: 77506
Number of successful extensions: 203
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 410448950
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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