Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P08_F_N19
         (884 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC191.09c |gst1||glutathione S-transferase Gst1|Schizosaccharo...    64   3e-11
SPCC965.07c |gst2||glutathione S-transferase Gst2|Schizosaccharo...    62   1e-10
SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyce...    54   3e-08
SPAC31G5.06 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    30   0.50 
SPCC1620.07c |||lunapark homolog|Schizosaccharomyces pombe|chr 3...    29   0.67 
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc...    26   6.2  

>SPCC191.09c |gst1||glutathione S-transferase
           Gst1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 229

 Score = 64.1 bits (149), Expect = 3e-11
 Identities = 46/139 (33%), Positives = 71/139 (51%), Gaps = 8/139 (5%)
 Frame = +2

Query: 203 EQLKPEYLKLNPQHTVPTLVD---DGLSIWESRAIITYLVNKYAKGSSL-YPEDPKARVL 370
           EQ  PE+L LNP   VPTL+D   +  +IWES AI+ YL +KY     +  P D      
Sbjct: 40  EQKSPEHLALNPNGRVPTLIDHHNNDYTIWESDAILIYLADKYDTERKISLPRDHPEYYK 99

Query: 371 VDQRLYFDI---GTLYQRFSDY-FYPQVFAGAPADKAKNEKVQEALQLLDKFLEGQKYVA 538
           V Q L+F     G ++ +   +  Y Q    +   + +NE ++  L +L+  L+ + Y+ 
Sbjct: 100 VIQYLFFQASGQGIIWGQAGWFSVYHQELVISAITRYRNE-IKRVLGVLEDILKDRDYLV 158

Query: 539 GPNLTVADLSLIASVSSLE 595
               T+ADLS I+  + LE
Sbjct: 159 ANRFTIADLSFISWNNFLE 177


>SPCC965.07c |gst2||glutathione S-transferase
           Gst2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 230

 Score = 62.1 bits (144), Expect = 1e-10
 Identities = 47/135 (34%), Positives = 75/135 (55%), Gaps = 10/135 (7%)
 Frame = +2

Query: 200 GEQLKPEYLKLNPQHTVPTLVD---DGLSIWESRAIITYLVNKY--AKGSSLYPEDPKAR 364
           GEQ   E+L LNP   VPTLVD   +  +IWES AI+ YL +KY   +  SL  +DP+  
Sbjct: 39  GEQKCKEHLALNPNGRVPTLVDHKNNDYTIWESDAILIYLADKYDTDRKISLSFDDPEYY 98

Query: 365 VLVDQRLYFDI---GTLYQR--FSDYFYPQVFAGAPADKAKNEKVQEALQLLDKFLEGQK 529
            L+ Q L+F     G ++ +  + ++F+ +    A   + +NE ++  L +L+  L+ + 
Sbjct: 99  KLI-QYLFFQASGQGVIWGQAGWFNFFHHEPVVSA-VTRYRNE-IKRVLGVLEDILKDRD 155

Query: 530 YVAGPNLTVADLSLI 574
           Y+     T+ADLS I
Sbjct: 156 YLVANKYTIADLSFI 170


>SPAC688.04c |gst3||glutathione S-transferase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 242

 Score = 54.0 bits (124), Expect = 3e-08
 Identities = 43/163 (26%), Positives = 65/163 (39%), Gaps = 17/163 (10%)
 Frame = +2

Query: 215 PEYLKLNPQHTVPTLVDDGLSIWESRAIITYLVNKYAKGSSLYPEDPKARVLVDQRLYFD 394
           P Y KL+P    P +VDDG++  ES AI+ +LV KY  G S  P +     L    L+  
Sbjct: 40  PAYTKLSPLGKSPIVVDDGVTYIESAAILEHLVRKY--GPSFKPSEEDVAELEKYELWMH 97

Query: 395 IG--------------TLYQRFSDYFYPQV---FAGAPADKAKNEKVQEALQLLDKFLEG 523
                            L    +  F+  +   F      K  +++    L  +D  L  
Sbjct: 98  FSEASLMPFIWASHVLDLSVNMTPIFFRYIVRQFVNGIKSKYLSKETFLNLDYIDNHLAS 157

Query: 524 QKYVAGPNLTVADLSLIASVSSLEASDIDFKKYANVKRWYETV 652
            +Y AG   T AD  +   + + +   +  K Y N+KRW   V
Sbjct: 158 NEYFAGEQFTAADPQMCFPIFAAQRDYLSQKPYKNIKRWMRVV 200


>SPAC31G5.06 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 234

 Score = 29.9 bits (64), Expect = 0.50
 Identities = 15/48 (31%), Positives = 30/48 (62%), Gaps = 1/48 (2%)
 Frame = +3

Query: 690 KDWRHSRDLSIXCLKSE-HLSTRNENLFKITSLELNLTN*AELKTSLL 830
           K+  H++ +S+ CL S+ +L   ++ +FK  ++ELN  N  ++K  +L
Sbjct: 58  KNHLHNKHVSLYCLLSDRYLQPPSKRIFKRWNMELNNLNCPDIKRYIL 105


>SPCC1620.07c |||lunapark homolog|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 334

 Score = 29.5 bits (63), Expect = 0.67
 Identities = 22/83 (26%), Positives = 34/83 (40%), Gaps = 4/83 (4%)
 Frame = +2

Query: 467 AKNEKVQEALQLLDKFLEGQ----KYVAGPNLTVADLSLIASVSSLEASDIDFKKYANVK 634
           A+  K+ +AL+   ++ E Q    KY   P L    LS  A+  S+  S        + +
Sbjct: 116 AEKRKILDALKSRKEYFETQALLEKYGEQPTLAQKKLSNAAAAKSVPGSSSSSSDPMHPQ 175

Query: 635 RWYETVKSTAPGYQEANXKGLEA 703
            WY+ V     G  E +    EA
Sbjct: 176 HWYDRVLEGLVGANENSENNREA 198


>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 721

 Score = 26.2 bits (55), Expect = 6.2
 Identities = 33/118 (27%), Positives = 51/118 (43%)
 Frame = +2

Query: 266 DGLSIWESRAIITYLVNKYAKGSSLYPEDPKARVLVDQRLYFDIGTLYQRFSDYFYPQVF 445
           + LSI ESR  I  +  ++   S L+P D K     + RL F +G  + R +      + 
Sbjct: 263 NSLSI-ESRLTIANMTTEWGALSGLFPTDEKLLAWYEDRLKF-LGPNHPRVNRETLDAIK 320

Query: 446 AGAPADKAKNEKVQEALQLLDKFLEGQKYVAGPNLTVADLSLIASVSSLEASDIDFKK 619
           A +P     +E    A  L+         V+GPN     + +  S ++LE  DI  KK
Sbjct: 321 A-SPI--LADEGAFYAKHLILDLSTLSPAVSGPN----SVKVYNSAATLEKKDILIKK 371


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,975,997
Number of Sequences: 5004
Number of extensions: 55701
Number of successful extensions: 184
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)