Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P08_F_J20
(825 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F3.09 |mug161||CwfJ family protein|Schizosaccharomyces pomb... 81 2e-16
SPAC17A5.02c |dbr1||RNA lariat debranching enzyme Dbr1 |Schizosa... 36 0.007
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 31 0.26
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 27 2.4
SPBC1348.05 |||membrane transporter|Schizosaccharomyces pombe|ch... 27 3.2
SPBPB2B2.16c |||MFS family membrane transporter |Schizosaccharom... 27 3.2
SPAC750.02c |||membrane transporter|Schizosaccharomyces pombe|ch... 27 3.2
SPBC17G9.11c |pyr1||pyruvate carboxylase|Schizosaccharomyces pom... 27 4.3
SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2 |Schizosacc... 26 5.6
SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 26 5.6
SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase |Schizo... 26 5.6
SPBC19F5.02c |||U3 snoRNP protein Utp4 |Schizosaccharomyces pomb... 26 7.5
>SPAC1F3.09 |mug161||CwfJ family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 561
Score = 81.0 bits (191), Expect = 2e-16
Identities = 69/256 (26%), Positives = 111/256 (43%), Gaps = 10/256 (3%)
Frame = +2
Query: 56 KMDKQKTLICGDVNGNFNALFTRVESIVKKSGQFDLLLCVGTFFGDDNS-QLDAYKMGTR 232
K+ L+ G +G + + K+ G F +C+G F + D K+
Sbjct: 6 KLKPADVLVIGSADGRVIEAIEYIADLHKQHG-FKFAICLGNLFSHKRTTSADVVKLKNE 64
Query: 233 KV--PVTTYVFGPSN---PDHV-HHYCEEGSEIVPNVIYMGRRGLFNTSTDVKIAYLTGL 394
KV P+ Y FG P+ + H G E+ PN+ MG G T IA L G
Sbjct: 65 KVKVPIPVY-FGVGTAGLPESIISHMAMYGPEVAPNLFCMGICGFMKTFYKFTIAQLGGS 123
Query: 395 SRRELGKDIPMCTYEPSDCSAVRDACFRG---QSEFRGVDVLITTLWPSGIQQDECQKVD 565
E P +E S + + CF Q + D+L ++ WP +Q++ +
Sbjct: 124 YNEEKYYQPPE-KFEQS----LNEKCFHRSDVQKLSKRCDILFSSEWPEDVQENSTLP-E 177
Query: 566 VEQDRLSDLLSWLAIHIKPRYHFVPSKDKFFERQPYRNQSMHQDYRECATRFIALXPVGN 745
+ + L+ LA + P+Y FVP ++ER+PY+N + T F+AL P N
Sbjct: 178 RKLPKGCMPLAALAANCMPQYFFVPGP-VYYEREPYKNSAAINVNTGTVTHFVALAPFKN 236
Query: 746 NLKEKWIYACSLQPIS 793
+ EK+ YA +L P++
Sbjct: 237 SKNEKFSYAFTLYPLT 252
>SPAC17A5.02c |dbr1||RNA lariat debranching enzyme Dbr1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 466
Score = 35.9 bits (79), Expect = 0.007
Identities = 43/191 (22%), Positives = 78/191 (40%), Gaps = 23/191 (12%)
Frame = +2
Query: 215 YKMGTRKVPVTTYVFGPSNPDHVHHYCEE---GSEIVPNVIYMGRRGLFNTSTDVKIAYL 385
Y G K P+ T G ++ +Y +E G + PN+ YMGR + N ++IA +
Sbjct: 76 YYNGRNKAPILTIFVGGNH--EASNYLDELPYGGWVAPNIYYMGRSSVINVG-GLRIAGI 132
Query: 386 TGLSRRELGKDIPMCTYE--PSDCSAVRDACFRGQSEF-------RGVDVLITTLWPSGI 538
+G+ D YE P + ++ + + + +D+ ++ WP GI
Sbjct: 133 SGIYS---AMDYKKGRYEGLPYNYKMLKSIYHTREFDVLSLKSLQKPIDIFLSHDWPRGI 189
Query: 539 QQ-DECQKV---------DVEQDRL-SDLLSWLAIHIKPRYHFVPSKDKFFERQPYRNQS 685
+Q + K+ +VE++ L S L L + +KPRY F + N
Sbjct: 190 EQHGDVAKLLRHKPFFRNEVERNDLGSPALEELLVELKPRYWMAAHLHTKFTAVVHHNSQ 249
Query: 686 MHQDYRECATR 718
C+++
Sbjct: 250 EDDGKLSCSSK 260
>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1811
Score = 30.7 bits (66), Expect = 0.26
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +3
Query: 624 DIILCHPKTSSLNGNHIEIRVCIKTIENVQHVLLHXL 734
D+I C K + LNGN C+ ++N++H L+ L
Sbjct: 1348 DLISCITKFAKLNGNQKFCLSCVDMLKNLEHELIKHL 1384
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 27.5 bits (58), Expect = 2.4
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = +1
Query: 16 QSNYQFKCLSLVYKNG*TENVNMW 87
Q+NY+F+ L KN EN N W
Sbjct: 185 QTNYEFELQKLTQKNSILENNNTW 208
>SPBC1348.05 |||membrane transporter|Schizosaccharomyces pombe|chr
2|||Manual
Length = 485
Score = 27.1 bits (57), Expect = 3.2
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = -1
Query: 204 CELSSPKNVPTHKSKSNCPD 145
C+ SS K+VP+ ++NCPD
Sbjct: 10 CDSSSIKHVPSTPLETNCPD 29
>SPBPB2B2.16c |||MFS family membrane transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 485
Score = 27.1 bits (57), Expect = 3.2
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = -1
Query: 204 CELSSPKNVPTHKSKSNCPD 145
C+ SS K+VP+ ++NCPD
Sbjct: 10 CDSSSIKHVPSTPLETNCPD 29
>SPAC750.02c |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 485
Score = 27.1 bits (57), Expect = 3.2
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = -1
Query: 204 CELSSPKNVPTHKSKSNCPD 145
C+ SS K+VP+ ++NCPD
Sbjct: 10 CDSSSIKHVPSTPLETNCPD 29
>SPBC17G9.11c |pyr1||pyruvate carboxylase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1185
Score = 26.6 bits (56), Expect = 4.3
Identities = 13/43 (30%), Positives = 23/43 (53%), Gaps = 3/43 (6%)
Frame = +2
Query: 650 KFFERQPYRNQSMHQDYRECAT---RFIALXPVGNNLKEKWIY 769
KFF +P N+ MH + + T +F+AL P+ ++ +Y
Sbjct: 1040 KFFLSRPEMNEEMHVEIDQGKTLIVKFVALGPLNPRTGQREVY 1082
>SPAC3G6.09c |tps2||trehalose-phosphate synthase Tps2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 849
Score = 26.2 bits (55), Expect = 5.6
Identities = 14/52 (26%), Positives = 27/52 (51%)
Frame = +2
Query: 44 HLCIKMDKQKTLICGDVNGNFNALFTRVESIVKKSGQFDLLLCVGTFFGDDN 199
H +++ K+ + +V+ N+ + V I+K+SG D + C G D+N
Sbjct: 723 HYDVEISPAKSFL--EVHPNYLNKGSIVRRILKRSGSVDFVFCAGDDKTDEN 772
>SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 504
Score = 26.2 bits (55), Expect = 5.6
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +1
Query: 34 KCLSLVYKNG*TENVNMWRCKWEFQCFV 117
KC + + KNG ++ +CK+EF C+V
Sbjct: 293 KCSTTIEKNGGCNHMTCKKCKYEF-CWV 319
>SPACUNK4.16c |||alpha,alpha-trehalose-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 944
Score = 26.2 bits (55), Expect = 5.6
Identities = 23/70 (32%), Positives = 32/70 (45%), Gaps = 6/70 (8%)
Frame = +2
Query: 107 NALFTRVESIVKKSGQFDLLLCVGTFFGDDNSQLDAYKMGTRKVPVTTY---VFGPSNPD 277
NA F S +S QFD L VGT +S D+ K +T + V P++ D
Sbjct: 209 NASFYNAISAATRSKQFDDHLFVGTCGIPTDSLPDSLKERISHDYITEHSSLVVYPTDTD 268
Query: 278 ---HVHHYCE 298
H +HYC+
Sbjct: 269 FVGHYNHYCK 278
>SPBC19F5.02c |||U3 snoRNP protein Utp4 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 710
Score = 25.8 bits (54), Expect = 7.5
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +2
Query: 2 GRPMINQITNLNAFHLCIKMDKQKTLICGDVNGN 103
G+P++NQ +N A D+ KTL G +G+
Sbjct: 100 GKPLVNQDSNAGAIWSIAICDETKTLAVGCDDGS 133
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,601,417
Number of Sequences: 5004
Number of extensions: 79024
Number of successful extensions: 280
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 224
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 280
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 404442380
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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