Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P08_F_I24
(859 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC26F1.12c |||conserved eukaryotic protein|Schizosaccharomyces... 79 1e-15
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 29 0.64
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 28 1.5
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 28 2.0
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 27 3.4
SPBC887.17 |||uracil permease |Schizosaccharomyces pombe|chr 2||... 27 3.4
SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar... 27 4.5
SPBC216.06c |swi1||replication fork protection complex subunit S... 27 4.5
SPAC27E2.03c |||GTP binding protein |Schizosaccharomyces pombe|c... 26 6.0
SPBC1604.15 |gpi16||pig-T |Schizosaccharomyces pombe|chr 2|||Manual 26 6.0
SPCC576.10c |rpt3||19S proteasome regulatory subunit Rpt3|Schizo... 26 7.9
SPAC26F1.08c |||conserved protein|Schizosaccharomyces pombe|chr ... 26 7.9
SPBP23A10.07 |rpa2||DNA-directed RNA polymerase I complex subuni... 26 7.9
>SPAC26F1.12c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 356
Score = 78.6 bits (185), Expect = 1e-15
Identities = 67/213 (31%), Positives = 110/213 (51%), Gaps = 14/213 (6%)
Frame = +3
Query: 258 EIAKHALLMLVNISAYTRGATELLKYKPLRYKNIIDLLISYVLNPNKTDADAACMTLSNI 437
+IA A+ LVN+S E ++ K L + L+ S V NP AD +CM L N+
Sbjct: 60 QIASQAVTALVNVSQ-----NEKVR-KVLMDDEFLQLIFSIVTNPLHGLADLSCMLLCNL 113
Query: 438 TRLED-----ELEVCLNTFIPHLN---DIMNAFV-NTEFN-KTGSNLHYLAPMFSNLACS 587
+ ED +++V L F N +M+ FV T+ +N +LA +F+++
Sbjct: 114 AKEEDFARILDMQVPLREFSLSKNIIDQLMDLFVKGTDHGINEYANFDFLANVFADMTRF 173
Query: 588 HRIRKWL--CEENPHV-PLIKLIPFCNYDVSNIRKGGAIGTIRNISFDTDYHEFLVSPD- 755
R RK+ +E HV P KL+ F + S +R+ G I+NISFD + + L+ +
Sbjct: 174 ERGRKYFTTLQEYDHVIPASKLVVFTEHK-SLLRRTGVAAIIKNISFDIPFQKVLMDEEG 232
Query: 756 LDLLTYILYPLMGNEDYPDDEMETLPVTLQYLP 854
+++L Y+L PL G E+ +++M+ + LQ LP
Sbjct: 233 INVLPYLLLPLAGPEELSEEDMDGMFDELQLLP 265
>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 611
Score = 29.5 bits (63), Expect = 0.64
Identities = 28/106 (26%), Positives = 52/106 (49%), Gaps = 1/106 (0%)
Frame = +3
Query: 219 TQAVIELTD-DRIKEIAKHALLMLVNISAYTRGATELLKYKPLRYKNIIDLLISYVLNPN 395
TQ +E++ D+ EI + +LV + ++ +G T ++ + Y++ ID S +
Sbjct: 251 TQIDLEMSFVDKPHEIMEVVEDLLVRLVSFAKGITLAKPFQHITYQHAIDKYGS-----D 305
Query: 396 KTDADAACMTLSNITRLEDELEVCLNTFIPHLNDIMNAFVNTEFNK 533
K D + L NIT L + + ++T I ND+ ++ N E K
Sbjct: 306 KPDIRFE-LPLKNITSLLPKQDPLISTEILVYNDLSHSLSNAESRK 350
>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1428
Score = 28.3 bits (60), Expect = 1.5
Identities = 20/74 (27%), Positives = 34/74 (45%), Gaps = 2/74 (2%)
Frame = +3
Query: 135 LRHIS-MDYLVSLSGTEDGVNKMLEHERITQAVIELTDDRIK-EIAKHALLMLVNISAYT 308
L H + +D+L + S +L I + +ELT + ++A H M +++ A
Sbjct: 797 LNHFTILDFLKTKSAKTPTSPAILTFNAIEKTKVELTWAHFELKVASHVEYMQLSVKAKA 856
Query: 309 RGATELLKYKPLRY 350
R LL Y PL +
Sbjct: 857 RSHILLLYYDPLEF 870
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 27.9 bits (59), Expect = 2.0
Identities = 26/108 (24%), Positives = 47/108 (43%), Gaps = 3/108 (2%)
Frame = +3
Query: 126 RIDLRHISMDYLVSLSGTEDGVNKMLEHERITQAVIELTDDRIKEIAKH--ALLMLVNIS 299
++ L H+ D+ L GT G L++E +L I K AL +V+ S
Sbjct: 498 QLPLAHLVEDFFGRLKGTTSGF-ATLDYEESDYVPADLVRLSIFMSGKSVDALCSIVHRS 556
Query: 300 -AYTRGATELLKYKPLRYKNIIDLLISYVLNPNKTDADAACMTLSNIT 440
A RG + + KPL K + ++++ V++ ++ N+T
Sbjct: 557 LALQRGREWIQRLKPLVPKQLYEVILQAVIDNRVVARESISALRKNVT 604
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 27.1 bits (57), Expect = 3.4
Identities = 21/85 (24%), Positives = 42/85 (49%), Gaps = 7/85 (8%)
Frame = +3
Query: 282 MLVNISAYTRGATE-LLKYKPLRYKNIIDLLISYVLNPNKTDADAA------CMTLSNIT 440
+LVN+ YT G+TE + K + ++ II L+I + + A+ + S ++
Sbjct: 1662 LLVNVVKYTEGSTEGTVSSKSVLFRKIIALIIGVFAELHNSMAEFVHQKTFFRLFSSILS 1721
Query: 441 RLEDELEVCLNTFIPHLNDIMNAFV 515
L+D +V + F+ + I+ F+
Sbjct: 1722 ELDDAKDVLESCFVDIYSVILECFL 1746
>SPBC887.17 |||uracil permease |Schizosaccharomyces pombe|chr
2|||Manual
Length = 625
Score = 27.1 bits (57), Expect = 3.4
Identities = 14/49 (28%), Positives = 28/49 (57%), Gaps = 3/49 (6%)
Frame = +3
Query: 117 PESRIDLRHISMDYLVSLSGTEDGVNKMLEHERITQ---AVIELTDDRI 254
P+ R + + +++ S S +EDG N+ HE +T ++ ++ D+RI
Sbjct: 543 PDDRKAMDNATLEMATSRSYSEDGKNEKTTHEDVTMKETSLKKMDDERI 591
>SPAC343.18 |rfp2||ubiquitin-protein ligase E3
Rfp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 205
Score = 26.6 bits (56), Expect = 4.5
Identities = 12/37 (32%), Positives = 22/37 (59%)
Frame = -1
Query: 169 RLTR*SMDMCLKSILDSGFRNSINSSKESSAIVCNRI 59
R TR +++L++GFRNS +++SS + R+
Sbjct: 93 RRTRNRSQTQRRTLLENGFRNSRKKAQDSSNSIAERV 129
>SPBC216.06c |swi1||replication fork protection complex subunit
Swi1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 971
Score = 26.6 bits (56), Expect = 4.5
Identities = 18/50 (36%), Positives = 24/50 (48%)
Frame = +3
Query: 396 KTDADAACMTLSNITRLEDELEVCLNTFIPHLNDIMNAFVNTEFNKTGSN 545
K A AC+ N + L DEL+V LN I I A N N+ G++
Sbjct: 705 KVAAVVACLINENKSDLLDELKVQLNCLISERKLITLADENKYINEGGND 754
>SPAC27E2.03c |||GTP binding protein |Schizosaccharomyces pombe|chr
1|||Manual
Length = 392
Score = 26.2 bits (55), Expect = 6.0
Identities = 17/55 (30%), Positives = 23/55 (41%), Gaps = 3/55 (5%)
Frame = +3
Query: 84 DSFDELIEFLKPESRIDLRHISMDYLVSLSGTEDGV---NKMLEHERITQAVIEL 239
+ FD L E KP+SR+ D G GV N L H R A+ ++
Sbjct: 68 ERFDWLCEAYKPKSRVPAFLTVFDIAGLTKGASTGVGLGNAFLSHVRAVDAIYQV 122
>SPBC1604.15 |gpi16||pig-T |Schizosaccharomyces pombe|chr 2|||Manual
Length = 545
Score = 26.2 bits (55), Expect = 6.0
Identities = 18/48 (37%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = +3
Query: 606 LCEENPHVPLIKLIPFCNYDVSNIRKGGAIGTI-RNISFDTDYHEFLV 746
+C EN PL KL+P C RK G + ++ FDTD+H F +
Sbjct: 181 VCTENLS-PLFKLLP-CK------RKAGIASLLDSHLFFDTDWHSFSI 220
>SPCC576.10c |rpt3||19S proteasome regulatory subunit
Rpt3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 389
Score = 25.8 bits (54), Expect = 7.9
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = +3
Query: 705 RNISFDTDYHEFLVSPDLDLLTYILYP 785
R + F T + L+SP++DL T+I+ P
Sbjct: 311 RRLVFQTITAKMLLSPEVDLDTFIMRP 337
>SPAC26F1.08c |||conserved protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 977
Score = 25.8 bits (54), Expect = 7.9
Identities = 10/32 (31%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = +3
Query: 540 SNLHYLAPMF-SNLACSHRIRKWLCEENPHVP 632
SN+ +++ + +L +H I KWL + H+P
Sbjct: 371 SNVDHMSSLILGSLKLAHAIMKWLVSDVEHIP 402
>SPBP23A10.07 |rpa2||DNA-directed RNA polymerase I complex subunit
Rpa2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1227
Score = 25.8 bits (54), Expect = 7.9
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = +2
Query: 77 GARFFRRIDRIPETRVQDRLETHIHGLSRQPIGDGRR 187
G +++R+ + + Q R IH L+RQP+ +R
Sbjct: 1067 GVVYYQRLRHMVSDKFQVRTTGPIHNLTRQPVKGRKR 1103
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,549,245
Number of Sequences: 5004
Number of extensions: 76092
Number of successful extensions: 233
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 223
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 232
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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