Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P08_F_G12
(842 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 53 4e-08
SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 45 2e-05
SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 41 3e-04
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 39 0.001
SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr ... 32 0.12
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 30 0.47
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 29 0.62
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 28 1.4
SPAC17G8.05 |med20||mediator complex subunit Med20|Schizosacchar... 28 1.9
SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces pomb... 28 1.9
SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharo... 28 1.9
SPAC926.03 |rlc1||myosin II regulatory light chain |Schizosaccha... 27 2.5
SPBC405.06 |||DNAJ protein Xdj1 |Schizosaccharomyces pombe|chr 2... 27 3.3
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 27 3.3
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 4.4
SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces pombe... 27 4.4
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 26 5.8
SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyce... 26 7.7
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 7.7
SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr... 26 7.7
SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate syntha... 26 7.7
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 53.2 bits (122), Expect = 4e-08
Identities = 39/136 (28%), Positives = 64/136 (47%), Gaps = 1/136 (0%)
Frame = +1
Query: 322 DDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSES 501
D DG+ + E ++ G +E +++ ++ D D +G+I EFL + M ++
Sbjct: 24 DQDGN--ITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDT 81
Query: 502 -RRNIVEQAFKKLDKTGDGAITIDDIKGVYSVDSQTRYKSGEETEDVIMKRFLANFESDG 678
V +AFK DK G+G IT++++ V + S S EE D+I E+D
Sbjct: 82 DNEEEVREAFKVFDKDGNGYITVEELTHV--LTSLGERLSQEEVADMIR-------EADT 132
Query: 679 TVDGKVTLEEFMNYYS 726
DG + EEF S
Sbjct: 133 DGDGVINYEEFSRVIS 148
Score = 37.1 bits (82), Expect = 0.003
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +1
Query: 310 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEF 468
F+ D DG+ + EE + + G L++ E ++ + DTD G I+ +EF
Sbjct: 91 FKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREADTDGDGVINYEEF 143
>SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 174
Score = 44.8 bits (101), Expect = 2e-05
Identities = 33/144 (22%), Positives = 67/144 (46%), Gaps = 6/144 (4%)
Frame = +1
Query: 193 EELMQKSARAMTQATDPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEFLYGI 372
E + ++ + + +++ L + AS L R+F +D+DG ++ +EF+ +
Sbjct: 24 ERIRKRFIKIDANQSGSIDRNEFLSIPSVASNPLA-SRLFSVVDEDGGGDVDFQEFINSL 82
Query: 373 KETGLELNKSEAEEL-FSQFDTDNSGSISLDEFLIKIRPPMSESRR-----NIVEQAFKK 534
+ NK E + F +D D G IS E + ++ + + R IV++ +
Sbjct: 83 SVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQIVDKTIME 142
Query: 535 LDKTGDGAITIDDIKGVYSVDSQT 606
+DK DG I+ ++ K + S + T
Sbjct: 143 VDKDRDGKISFEEFKDIVSGSNVT 166
Score = 42.3 bits (95), Expect = 8e-05
Identities = 37/145 (25%), Positives = 68/145 (46%), Gaps = 1/145 (0%)
Frame = +1
Query: 310 FRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLIKIRP- 486
F ++D + S +++ EFL I + A LFS D D G + EF+ +
Sbjct: 30 FIKIDANQSGSIDRNEFL-SIPSVA---SNPLASRLFSVVDEDGGGDVDFQEFINSLSVF 85
Query: 487 PMSESRRNIVEQAFKKLDKTGDGAITIDDIKGVYSVDSQTRYKSGEETEDVIMKRFLANF 666
+ ++ ++ AFK D DG I+ ++ V + T + ++ + ++ K +
Sbjct: 86 SVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLRE-DQLQQIVDKTIM--- 141
Query: 667 ESDGTVDGKVTLEEFMNYYSGISVS 741
E D DGK++ EEF + SG +V+
Sbjct: 142 EVDKDRDGKISFEEFKDIVSGSNVT 166
>SPAC18B11.04 |ncs1||related to neuronal calcium sensor
Ncs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 190
Score = 40.7 bits (91), Expect = 3e-04
Identities = 37/129 (28%), Positives = 60/129 (46%), Gaps = 6/129 (4%)
Frame = +1
Query: 343 LNKEEFLYGIKETGLELNKSE-AEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVE 519
LNK EF K+ + S AE +F+ FD D +G I EF+ + + +
Sbjct: 43 LNKSEFQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFICALSVTSRGELNDKLI 102
Query: 520 QAFKKLDKTGDGAITIDD----IKGVYS-VDSQTRYKSGEETEDVIMKRFLANFESDGTV 684
AF+ D +G I+ D+ + +Y V S + E+T + + + + N D
Sbjct: 103 WAFQLYDLDNNGLISYDEMLRIVDAIYKMVGSMVKLPEDEDTPEKRVNK-IFNM-MDKNK 160
Query: 685 DGKVTLEEF 711
DG++TLEEF
Sbjct: 161 DGQLTLEEF 169
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 38.7 bits (86), Expect = 0.001
Identities = 26/140 (18%), Positives = 60/140 (42%), Gaps = 1/140 (0%)
Frame = +1
Query: 298 LGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFL-I 474
+ F+ D D ++ E ++ G KSE ++ FD G + +++F+ +
Sbjct: 39 INEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQMEDFVRV 98
Query: 475 KIRPPMSESRRNIVEQAFKKLDKTGDGAITIDDIKGVYSVDSQTRYKSGEETEDVIMKRF 654
+ +++AF+ D G I++ +++ V + E +D ++
Sbjct: 99 MTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAK-------ELNENIDDQELEAM 151
Query: 655 LANFESDGTVDGKVTLEEFM 714
+ F+ D DG++ +EF+
Sbjct: 152 IEEFDLD--QDGEINEQEFI 169
Score = 37.1 bits (82), Expect = 0.003
Identities = 29/121 (23%), Positives = 53/121 (43%)
Frame = +1
Query: 388 ELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVEQAFKKLDKTGDGAITI 567
E + + E F FD+D +I E +R + ++ V + + DKTG G + +
Sbjct: 33 EEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRDFDKTGKGYLQM 92
Query: 568 DDIKGVYSVDSQTRYKSGEETEDVIMKRFLANFESDGTVDGKVTLEEFMNYYSGISVSID 747
+D V + R + E++ KR F+ D T GK++L ++ +ID
Sbjct: 93 EDFVRVMTEKIVER----DPLEEI--KRAFELFDDDET--GKISLRNLRRVAKELNENID 144
Query: 748 N 750
+
Sbjct: 145 D 145
>SPAC15A10.08 |ain1||alpha-actinin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 621
Score = 31.9 bits (69), Expect = 0.12
Identities = 22/96 (22%), Positives = 40/96 (41%), Gaps = 2/96 (2%)
Frame = +1
Query: 295 GLGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLI 474
G+ ++FR + S +LN+ EF + GL + E LF + G ++ + F
Sbjct: 488 GITKVFRHFEKKKSNMLNEVEFYAALASLGLVYDTEEGTALFHRAANSEEG-VTYERFTE 546
Query: 475 KIRPPMSESRRNIVEQAFKKLDKTGDGA--ITIDDI 576
+ + + R+ Q DG +T DD+
Sbjct: 547 IVMEELED--RDSARQVLYAFCDVADGKSYVTSDDL 580
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 29.9 bits (64), Expect = 0.47
Identities = 31/114 (27%), Positives = 55/114 (48%), Gaps = 2/114 (1%)
Frame = +1
Query: 385 LELNKSEA--EELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVEQAFKKLDKTGDGA 558
L LN S EE F + D D+SG +S +EF + + ++R IV+ FK+ +G
Sbjct: 326 LHLNASMEFLEETFQKADADHSGKLSFEEFQHFV--SLLKTRSEIVD-IFKEY-TSGSDK 381
Query: 559 ITIDDIKGVYSVDSQTRYKSGEETEDVIMKRFLANFESDGTVDGKVTLEEFMNY 720
++++ + S + R S D I +++ +D D K+ L EF ++
Sbjct: 382 MSLEQFRHFLSTSQKARLDS-----DSIRTLYVSFCSND---DSKMGLIEFTSF 427
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 29.5 bits (63), Expect = 0.62
Identities = 18/74 (24%), Positives = 31/74 (41%)
Frame = +1
Query: 355 EFLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVEQAFKK 534
E+ G+ + + S LF +FD +GS+SL + + I + F+
Sbjct: 564 EWAKGLDAAAINNSSSFLRHLFLRFDKSMTGSLSLQDLVSGIAELKFRDVMRNISFIFEL 623
Query: 535 LDKTGDGAITIDDI 576
D GDG + D+
Sbjct: 624 YDFNGDGFMDKPDV 637
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 28.3 bits (60), Expect = 1.4
Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 1/58 (1%)
Frame = +2
Query: 398 KVKPKNSSVNSTQTIVAQSVLMNSLLKSVLLCRNRVVT-L*NKHSRSLTRLVTVQSQL 568
K+ KN+ S + + ++ L L L + +++ L NK S TRL +QSQL
Sbjct: 765 KLNKKNADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEESTRLQELQSQL 822
>SPAC17G8.05 |med20||mediator complex subunit
Med20|Schizosaccharomyces pombe|chr 1|||Manual
Length = 180
Score = 27.9 bits (59), Expect = 1.9
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +1
Query: 592 VDSQTRYKSGEETEDVIMKRFLANFESDGTVDGKV 696
VD T + E ED+I++ L NF T++G +
Sbjct: 55 VDEATMIDAEPELEDIIVRTKLWNFRQSFTIEGSI 89
>SPBC1709.03 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 398
Score = 27.9 bits (59), Expect = 1.9
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = +1
Query: 553 GAITIDDIKGVYSVDSQTRYKSGEETEDV 639
G I+I D GVYS + T YKS E+ E +
Sbjct: 126 GVISIHDSTGVYSQITTTPYKSLEDYEQL 154
>SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1369
Score = 27.9 bits (59), Expect = 1.9
Identities = 38/151 (25%), Positives = 61/151 (40%), Gaps = 11/151 (7%)
Frame = +1
Query: 238 DPLEKLRLLCLSRGASGILGLGRIFRRMDDDGSKLLNKEEFLYGIKETGLELNKSEAEEL 417
D E+ + S +S + L I + GS+L E F I G+ + AE
Sbjct: 793 DNYEQAESIISSLSSSALSELSYISESSMNIGSRL--DERF---IDANGVAIRDFSAE-- 845
Query: 418 FSQFDTDNS-GSISLDEFLIKIRPPMSESRRNIVEQAFKK--------LDKTGDGAITID 570
+ +NS G +S+D FL K++ + + + F+K DK D
Sbjct: 846 LTYLTPENSKGKLSIDHFLNKVQSRWHDEEHHYYKTGFRKRVYKYLKIKDKKSKDVDPDD 905
Query: 571 DIKGVYSVDSQT--RYKSGEETEDVIMKRFL 657
D+ +++ T RYKS T I +R L
Sbjct: 906 DLVNQLPLNAYTKPRYKSAASTRLNIYQRIL 936
>SPAC926.03 |rlc1||myosin II regulatory light chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 27.5 bits (58), Expect = 2.5
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +1
Query: 490 MSESRRNIVEQAFKKLDKTGDGAITIDDIK 579
++ S+ +++AF LDK GDG I +D+K
Sbjct: 42 LTSSQIQELKEAFALLDKDGDGNIGREDVK 71
>SPBC405.06 |||DNAJ protein Xdj1 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 413
Score = 27.1 bits (57), Expect = 3.3
Identities = 13/36 (36%), Positives = 22/36 (61%)
Frame = +1
Query: 571 DIKGVYSVDSQTRYKSGEETEDVIMKRFLANFESDG 678
D+ G+ S DS +++ G +DV+ + F NFE+ G
Sbjct: 70 DMYGMNS-DSNSQFDGGVNLDDVLAQMFGMNFEAGG 104
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 27.1 bits (57), Expect = 3.3
Identities = 11/45 (24%), Positives = 22/45 (48%)
Frame = -3
Query: 186 DGTSRHWSVPCN*TLHFSFYFWRILVVEARVFGILRRRSASRHQS 52
+G S++W VPC H + FW + + + +++ + QS
Sbjct: 257 NGVSQNWHVPC----HMIYKFWNVKLCQKTSIETSKKKDSELSQS 297
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.6 bits (56), Expect = 4.4
Identities = 20/101 (19%), Positives = 42/101 (41%), Gaps = 3/101 (2%)
Frame = +1
Query: 388 ELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVEQAFKK---LDKTGDGA 558
+L ++E E +++ D + GS+ DEF + + + N V T +
Sbjct: 853 KLTRTELEHIWNLCDHGDKGSLDRDEFAVALHLIYRKLNGNEVPAVLPPELIPPSTRNFT 912
Query: 559 ITIDDIKGVYSVDSQTRYKSGEETEDVIMKRFLANFESDGT 681
+++ +K + D+ R G E + + K + S+ T
Sbjct: 913 ESLNQVKNLIKNDTSNRKPFGAENQSKLKKNSFYDNPSETT 953
>SPCC613.04c |rng3||UCS-domain protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 746
Score = 26.6 bits (56), Expect = 4.4
Identities = 10/34 (29%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -2
Query: 367 HKGIL-LCSVVYFHHHPCDGKFFQVPRSLKHLET 269
H+G++ +C++VY KF + P++++ L T
Sbjct: 686 HRGLVCICNIVYSKDQEIFNKFIKTPKAVETLRT 719
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 26.2 bits (55), Expect = 5.8
Identities = 21/71 (29%), Positives = 32/71 (45%)
Frame = +1
Query: 358 FLYGIKETGLELNKSEAEELFSQFDTDNSGSISLDEFLIKIRPPMSESRRNIVEQAFKKL 537
F+ +E+ LE E FSQ ++ + SL+ I P SRR IVEQ ++
Sbjct: 680 FILPNEESLLEKYWINYNESFSQLSRESLFT-SLESPFTDIESPTIVSRRKIVEQRKLRM 738
Query: 538 DKTGDGAITID 570
+K +D
Sbjct: 739 EKESFQETNVD 749
>SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 964
Score = 25.8 bits (54), Expect = 7.7
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = -3
Query: 705 FKSNLPIDCSIGLKIREEPLHYYIFSFF 622
++S+ P S+G E L YYI+ +F
Sbjct: 19 YESSAPASSSLGNSHPNESLDYYIYDYF 46
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 7.7
Identities = 10/34 (29%), Positives = 17/34 (50%)
Frame = -3
Query: 561 DCTVTSLVKLLECLFYNVTTRFRHRRTDFNKEFI 460
D TV + +E LF+N+T +H F + +
Sbjct: 699 DATVANRKDFIEFLFHNITVSSKHTAVIFTSDLL 732
>SPCC825.02 |||glucosidase II Gtb1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 506
Score = 25.8 bits (54), Expect = 7.7
Identities = 11/32 (34%), Positives = 14/32 (43%)
Frame = +3
Query: 138 NGEFSCMAQTNVCWFRPGGRADAKECSCYDTS 233
NG+F C + + P R D C C D S
Sbjct: 81 NGKFFCKNTGYISSYIPSNRVDDTVCDCCDGS 112
>SPBC418.01c |his4|SPBC887.20c|imidazoleglycerol-phosphate
synthase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 541
Score = 25.8 bits (54), Expect = 7.7
Identities = 17/55 (30%), Positives = 23/55 (41%)
Frame = -3
Query: 831 C*SETGKVXSQALELVRLTHH*VKIAVVVNGDADATVVVHELFKSNLPIDCSIGL 667
C + G +ELVRL + V I V+ + A E+FK DC L
Sbjct: 462 CMDQDGSNAGYDIELVRLVKNSVNIPVIASSGAGIPQHFEEVFKET---DCDAAL 513
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,273,050
Number of Sequences: 5004
Number of extensions: 66512
Number of successful extensions: 255
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 233
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 251
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 416455520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -