Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P08_F_E15
(794 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC106.20 |exo70|SPBC582.02|exocyst complex subunit Exo70 |Schi... 31 0.25
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 29 1.0
SPAC4H3.03c |||glucan 1,4-alpha-glucosidase |Schizosaccharomyces... 28 1.3
SPCC285.09c |cgs2|pde1|cAMP-specific phosphodiesterase Cgs2|Schi... 28 1.8
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 27 3.1
SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces ... 27 4.1
SPBC1289.05c |vma10||V-type ATPase subunit G|Schizosaccharomyces... 27 4.1
SPBC8D2.14c |sed5||SNARE Sed5 |Schizosaccharomyces pombe|chr 2||... 27 4.1
SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|... 26 5.4
SPCC4G3.14 |mdj1||DNAJ domain protein Mdj1 |Schizosaccharomyces ... 26 5.4
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 26 5.4
SPAC8F11.05c |mug130||sequence orphan|Schizosaccharomyces pombe|... 26 7.1
SPBC1348.10c |||phospholipase |Schizosaccharomyces pombe|chr 2||... 26 7.1
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 26 7.1
SPAC1687.20c |mis6||inner centromere protein Mis6|Schizosaccharo... 26 7.1
SPAC20G4.03c |hri1||eIF2 alpha kinase Hri1|Schizosaccharomyces p... 26 7.1
SPAC977.09c |||phospholipase |Schizosaccharomyces pombe|chr 1|||... 26 7.1
SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase |Schizosac... 25 9.4
SPBC336.04 |cdc6|pol3, pold, mis10|DNA polymerase delta catalyti... 25 9.4
SPCC1795.01c |mad3|SPCC895.02|mitotic spindle checkpoint protein... 25 9.4
SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces ... 25 9.4
>SPBC106.20 |exo70|SPBC582.02|exocyst complex subunit Exo70
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 615
Score = 30.7 bits (66), Expect = 0.25
Identities = 23/100 (23%), Positives = 42/100 (42%), Gaps = 1/100 (1%)
Frame = +3
Query: 210 FVDFVKEKQLDGRKIADVTEGIVKLWRPQSNAKKFIAFLEDLKRDPDKYLNTSESI-DDK 386
F F K + ++D + ++ + + S + L D D Y S +I D
Sbjct: 13 FETFQKNLNSVAKNVSDASNILLSMDKRLSGLEASAGILRD---DVTNYNRVSSNIYDTL 69
Query: 387 SEAEMALIESQYQTVKKKGTEELGNVNNSIEDLLKKLVPV 506
E E + + V +KG +E N+N S+ LK ++ +
Sbjct: 70 KEMESLQVIHSHLPVLQKGLQECQNLNKSVSQNLKSVMDI 109
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 28.7 bits (61), Expect = 1.0
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = -2
Query: 283 SFTIPSVTSAIFRPSSCFSLTKSTNSTKSHVCNNFTTPSVSKQSSF 146
S TI S +S+ P+S S S++S+ S + ++ S+S SSF
Sbjct: 287 SSTISSSSSSSSSPTSTSSTISSSSSSSSSFSSTLSSSSMSSSSSF 332
>SPAC4H3.03c |||glucan 1,4-alpha-glucosidase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 649
Score = 28.3 bits (60), Expect = 1.3
Identities = 20/85 (23%), Positives = 38/85 (44%)
Frame = +3
Query: 375 IDDKSEAEMALIESQYQTVKKKGTEELGNVNNSIEDLLKKLVPVKSFLYRNQPKQINKAA 554
+D + E A+++S Y +KG NN I D ++P+ SF+ P+ ++
Sbjct: 473 MDTRDEIYEAIMDSGYNM--EKGFFAQSFENNDILDASVLVMPLVSFISPTDPRFLSTMD 530
Query: 555 PSYLPMKPVSPKRSKKFFRLNSYEY 629
P++ + FR N++ Y
Sbjct: 531 NIMKPLEKDGLMSNGLIFRYNNFVY 555
>SPCC285.09c |cgs2|pde1|cAMP-specific phosphodiesterase
Cgs2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 346
Score = 27.9 bits (59), Expect = 1.8
Identities = 18/61 (29%), Positives = 25/61 (40%), Gaps = 3/61 (4%)
Frame = -2
Query: 331 SSRNAMNFLALDCGLHSFTIPSVTSAIF---RPSSCFSLTKSTNSTKSHVCNNFTTPSVS 161
S + FL C L + SA+F P + L ++ K HV NN PS+
Sbjct: 103 SEQRIKTFLITHCHLDHIYGAVINSAMFGPQNPRTIVGLNYVIDTLKKHVFNNLLWPSLD 162
Query: 160 K 158
K
Sbjct: 163 K 163
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 27.1 bits (57), Expect = 3.1
Identities = 12/42 (28%), Positives = 22/42 (52%)
Frame = -1
Query: 224 NEVHKLNQVPCLQ*LHNTLCIQAIQFFISVLYTHYDSTTTNI 99
N +HK +++ CL +HN L Q + S+++ S N+
Sbjct: 3981 NHLHKNSRIVCLSEIHNQLPHQLLCISRSIVFNKQTSFKNNL 4022
>SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 582
Score = 26.6 bits (56), Expect = 4.1
Identities = 13/39 (33%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
Frame = -2
Query: 274 IPSVTSAIFR--PSSCFSLTKSTNSTKSHVCNNFTTPSV 164
+PS T+ + + PS C +++ ST S + +N + PSV
Sbjct: 284 VPSYTTLVSQLPPSPCLTVSSGPLSTASSIPSNCSCPSV 322
>SPBC1289.05c |vma10||V-type ATPase subunit G|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 108
Score = 26.6 bits (56), Expect = 4.1
Identities = 20/64 (31%), Positives = 31/64 (48%)
Frame = +3
Query: 261 VTEGIVKLWRPQSNAKKFIAFLEDLKRDPDKYLNTSESIDDKSEAEMALIESQYQTVKKK 440
V IV+ R + A LE KR+ D+Y + E KSE++ + I SQ + KK
Sbjct: 18 VARNIVEKARQHRTQRLKDARLE-AKREIDEYASKKEEEFKKSESQASGIYSQAEAESKK 76
Query: 441 GTEE 452
++
Sbjct: 77 QVQD 80
>SPBC8D2.14c |sed5||SNARE Sed5 |Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.6 bits (56), Expect = 4.1
Identities = 13/43 (30%), Positives = 25/43 (58%)
Frame = +3
Query: 357 LNTSESIDDKSEAEMALIESQYQTVKKKGTEELGNVNNSIEDL 485
LN + + + E +MAL+ESQ T ++ + N+ ++I +L
Sbjct: 197 LNLGDGANTRYE-QMALLESQTDTYSQQRMSSIQNIESTITEL 238
>SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein
5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 736
Score = 26.2 bits (55), Expect = 5.4
Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 2/62 (3%)
Frame = +3
Query: 360 NTSESIDDK--SEAEMALIESQYQTVKKKGTEELGNVNNSIEDLLKKLVPVKSFLYRNQP 533
N ESI S ++ L +YQT +K E N+SI + + K+F + QP
Sbjct: 164 NKKESISSSTNSNIKLHLAARKYQTKPEKFNE-----NSSISQVTSAIKATKAFSHLTQP 218
Query: 534 KQ 539
K+
Sbjct: 219 KR 220
>SPCC4G3.14 |mdj1||DNAJ domain protein Mdj1 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 528
Score = 26.2 bits (55), Expect = 5.4
Identities = 17/58 (29%), Positives = 24/58 (41%)
Frame = -2
Query: 295 CGLHSFTIPSVTSAIFRPSSCFSLTKSTNSTKSHVCNNFTTPSVSKQSSFLFLSCTLI 122
CGLHSFT S +F S S + N S C F ++ + + F C +
Sbjct: 11 CGLHSFT-NSSAQQLFSKSIAHSSRR--NFVISSSCTKFRNVAIQRNAKREFSRCAAL 65
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 26.2 bits (55), Expect = 5.4
Identities = 22/78 (28%), Positives = 38/78 (48%)
Frame = +3
Query: 327 EDLKRDPDKYLNTSESIDDKSEAEMALIESQYQTVKKKGTEELGNVNNSIEDLLKKLVPV 506
E L D + YL+ ++ ID SEA +++ T+ + GN +NS LK + +
Sbjct: 113 EHLGADTESYLSANDFIDSTSEALTKIVD--VDTL----SAPFGNDSNSRPHSLKNVEKL 166
Query: 507 KSFLYRNQPKQINKAAPS 560
+F + P I++ PS
Sbjct: 167 HTF---SAPYTISEETPS 181
>SPAC8F11.05c |mug130||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 241
Score = 25.8 bits (54), Expect = 7.1
Identities = 12/42 (28%), Positives = 20/42 (47%)
Frame = +3
Query: 564 LPMKPVSPKRSKKFFRLNSYEYPLFDLRSRLKQETTDKYYPM 689
L + P+S K K FR N +++S + +KY P+
Sbjct: 80 LYLSPISKKSEKLIFRFNLNHRGYLEVKSGFRAFIENKYSPL 121
>SPBC1348.10c |||phospholipase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 673
Score = 25.8 bits (54), Expect = 7.1
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +3
Query: 582 SPKRSKKFFRLNSYEYPLFDLRSRLKQET 668
S R++ +F+ Y YP+ SRL++ET
Sbjct: 268 SSMRNQSWFQNADYPYPIIVADSRLEEET 296
>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2310
Score = 25.8 bits (54), Expect = 7.1
Identities = 17/49 (34%), Positives = 22/49 (44%)
Frame = -1
Query: 365 CIQIFIWVAF*VLEECNEFLSIRLWSP*LYYPFCNICDFPSIQLFFFNE 219
CI+ +W A V E L + S L+ P I DFP L F N+
Sbjct: 684 CIEFLLWAALLVEPFPFELLRLITTSMHLFIPKEEILDFPLNVLQFDND 732
>SPAC1687.20c |mis6||inner centromere protein
Mis6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 672
Score = 25.8 bits (54), Expect = 7.1
Identities = 19/67 (28%), Positives = 30/67 (44%), Gaps = 1/67 (1%)
Frame = -2
Query: 253 IFRPSSCFSLTKSTNSTKSHVCNNFTTPSVSKQSSFLF-LSCTLIMTQLQQIYLKILXKT 77
+FR + + N T C T + S F + +S +++L Q+Y KIL K
Sbjct: 423 LFRMLNLRIASMGNNHTLLEKCLLITNHATFLVSHFSWDVSLAYQLSRLFQLYYKILTKI 482
Query: 76 HKIFEPH 56
K EP+
Sbjct: 483 RKQIEPN 489
>SPAC20G4.03c |hri1||eIF2 alpha kinase Hri1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 704
Score = 25.8 bits (54), Expect = 7.1
Identities = 15/44 (34%), Positives = 20/44 (45%), Gaps = 1/44 (2%)
Frame = -2
Query: 313 NFLA-LDCGLHSFTIPSVTSAIFRPSSCFSLTKSTNSTKSHVCN 185
NF+ +D GL T PS +F+ S STK+ CN
Sbjct: 321 NFMFDMDTGLLQHTYPSSVQILFQEDSVADDLTPCYSTKNSTCN 364
>SPAC977.09c |||phospholipase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 673
Score = 25.8 bits (54), Expect = 7.1
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +3
Query: 582 SPKRSKKFFRLNSYEYPLFDLRSRLKQET 668
S R++ +F+ Y YP+ SRL++ET
Sbjct: 268 SSMRNQSWFQNADYPYPIIVADSRLEEET 296
>SPBP35G2.12 |||nucleoside diphosphate-sugar hydrolase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 205
Score = 25.4 bits (53), Expect = 9.4
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +1
Query: 214 WTSLKKNNWMDGKSQM 261
WTSLKK W D K ++
Sbjct: 22 WTSLKKIRWQDEKGKI 37
>SPBC336.04 |cdc6|pol3, pold, mis10|DNA polymerase delta catalytic
subunit Cdc6 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1086
Score = 25.4 bits (53), Expect = 9.4
Identities = 16/55 (29%), Positives = 25/55 (45%)
Frame = -1
Query: 263 NICDFPSIQLFFFNEVHKLNQVPCLQ*LHNTLCIQAIQFFISVLYTHYDSTTTNI 99
NIC+F L + +++ P L +HN + F S Y +S TT+I
Sbjct: 381 NICNFDIPYLLDRAKSLRIHNFPLLGRIHNFFSVAKETTFSSKAYGTRESKTTSI 435
>SPCC1795.01c |mad3|SPCC895.02|mitotic spindle checkpoint protein
Mad3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 310
Score = 25.4 bits (53), Expect = 9.4
Identities = 15/65 (23%), Positives = 34/65 (52%)
Frame = +3
Query: 189 QTWDLVEFVDFVKEKQLDGRKIADVTEGIVKLWRPQSNAKKFIAFLEDLKRDPDKYLNTS 368
+ W ++ ++ KE ++ RK + K +++ +K +A L+ + ++ + TS
Sbjct: 8 KNWVHMDVIEQSKEN-IEPRKAGHSASALAKS-SSRNHTEKEVAGLQKERMGHERKIETS 65
Query: 369 ESIDD 383
ES+DD
Sbjct: 66 ESLDD 70
>SPAC144.14 |klp8||kinesin-like protein Klp8|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 511
Score = 25.4 bits (53), Expect = 9.4
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -3
Query: 93 KSSTKHTKYSNRTNEPRT 40
K KH+++ NR N PRT
Sbjct: 43 KHGLKHSRHKNRVNGPRT 60
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,425,996
Number of Sequences: 5004
Number of extensions: 75638
Number of successful extensions: 259
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 245
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 259
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 387388442
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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