Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P08_F_D21
(838 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 30 0.47
SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||M... 30 0.47
SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon... 29 0.62
SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16 |Schizosa... 28 1.4
SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa... 27 2.5
SPBC530.15c ||SPBC661.01|spermidine family transporter |Schizosa... 26 5.8
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 26 5.8
SPAC4D7.09 |tif223||translation initiation factor eIF2B|Schizosa... 26 5.8
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 26 7.6
SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase Mpg1|Sc... 26 7.6
SPAC8F11.03 |msh3|swi4|MutS protein homolog 3|Schizosaccharomyce... 26 7.6
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 29.9 bits (64), Expect = 0.47
Identities = 13/41 (31%), Positives = 22/41 (53%)
Frame = +2
Query: 593 IEDNSVLPAETIVPSFARYSGSPAHLITTLPEAMPDLMTEF 715
+ED +LPA + + S P + T+P +P ++TEF
Sbjct: 646 MEDTRLLPASLELANTQGASNPPTSALPTVPVPLPSIITEF 686
>SPAC11D3.15 |||oxoprolinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1317
Score = 29.9 bits (64), Expect = 0.47
Identities = 14/43 (32%), Positives = 24/43 (55%), Gaps = 1/43 (2%)
Frame = -2
Query: 435 FLKGGRITEPLLIIQNLPVLTLAKSP-LMMASLCTITLPCKTI 310
+ +GGR+ + + NLPV T+ P L++ TI +P K +
Sbjct: 676 YFEGGRVPSEVYSLDNLPVGTIVNGPSLIVDKTQTIVVPPKAV 718
>SPAC8C9.15c |tif225||translation initiation factor eIF2B epsilon
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 678
Score = 29.5 bits (63), Expect = 0.62
Identities = 23/92 (25%), Positives = 43/92 (46%), Gaps = 5/92 (5%)
Frame = +2
Query: 371 NVKTGRFCIISKGSVIRPPFKKFSKGVAFFPLQMGDHVFVGENTVVNAAVVGSYVYIGKN 550
+V R CII ++I K V + +G + +G N +++A + V IG N
Sbjct: 318 DVVLARSCIIKARTLIGAYTKVGDASVVANTI-IGRNCTIGSNCSIDSAFLWEDVVIGDN 376
Query: 551 VVIGRRCV-----LKDCCMIEDNSVLPAETIV 631
IG+ + + + C IED +++ A ++
Sbjct: 377 CRIGKAILANSVKIGNNCSIEDGAIVAAGVVI 408
Score = 29.1 bits (62), Expect = 0.82
Identities = 14/59 (23%), Positives = 30/59 (50%)
Frame = +2
Query: 476 DHVFVGENTVVNAAVVGSYVYIGKNVVIGRRCVLKDCCMIEDNSVLPAETIVPSFARYS 652
+ V +G+N + A++ + V IG N I ++ +I DN+++ + +F +S
Sbjct: 369 EDVVIGDNCRIGKAILANSVKIGNNCSIEDGAIVAAGVVIGDNTIIEKNKRLTTFESHS 427
>SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 759
Score = 28.3 bits (60), Expect = 1.4
Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 3/55 (5%)
Frame = +2
Query: 593 IEDNSVLPAETIVPSFARYSGSPAHLITTLPEAMPDLMTEFT---KSYYQHFLPT 748
++D S++ TI+ S+++Y G+ L L +P+ M FT KS Y PT
Sbjct: 664 LKDRSIVTKTTIM-SWSKYQGTKESLFQFLSIYIPECMLPFTKLLKSIYSPDCPT 717
>SPCC550.03c |||RNA helicase involved in mRNA
catabolism|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1213
Score = 27.5 bits (58), Expect = 2.5
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = +2
Query: 452 AFFPLQMGDHVFVGENTVVNAAVVGSY 532
A + L+MGD VFV +T VV Y
Sbjct: 288 AIYHLEMGDSVFVAAHTSAGKTVVAEY 314
>SPBC530.15c ||SPBC661.01|spermidine family transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 516
Score = 26.2 bits (55), Expect = 5.8
Identities = 14/54 (25%), Positives = 23/54 (42%)
Frame = +2
Query: 311 IVLHGKVIVQSDAIIRGDLANVKTGRFCIISKGSVIRPPFKKFSKGVAFFPLQM 472
I+ H + ++ IR V TG +C+ +K F F FPL++
Sbjct: 248 IIFHRETYTRTITEIRASKVRVLTGNYCLHAKSEEEPLEFSYFFHKYFTFPLRL 301
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 26.2 bits (55), Expect = 5.8
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = -2
Query: 393 QNLPVLTLAKSPLMMASLCTITLP 322
Q + VL+LA L+MAS C ++LP
Sbjct: 1039 QMVQVLSLATFTLLMASTCIMSLP 1062
>SPAC4D7.09 |tif223||translation initiation factor
eIF2B|Schizosaccharomyces pombe|chr 1|||Manual
Length = 468
Score = 26.2 bits (55), Expect = 5.8
Identities = 11/38 (28%), Positives = 22/38 (57%)
Frame = +2
Query: 488 VGENTVVNAAVVGSYVYIGKNVVIGRRCVLKDCCMIED 601
+ +N+ + +++G IGK VV+ +L D ++ED
Sbjct: 378 IKDNSNIKKSIIGKNCVIGKGVVVS-NSILMDNIVVED 414
>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1428
Score = 25.8 bits (54), Expect = 7.6
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = -2
Query: 663 AGLPEYLAKEGTIVSAGRTELSSIMQQSLSTHLLPI 556
AG+P L K+ + + G +I Q SLS ++P+
Sbjct: 1041 AGVPTLLIKQENLCNNGSLLFEAIEQNSLSKVMIPL 1076
>SPCC1906.01 |mpg1||mannose-1-phosphate guanyltransferase
Mpg1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 363
Score = 25.8 bits (54), Expect = 7.6
Identities = 26/109 (23%), Positives = 46/109 (42%)
Frame = +2
Query: 302 SQNIVLHGKVIVQSDAIIRGDLANVKTGRFCIISKGSVIRPPFKKFSKGVAFFPLQMGDH 481
S NI+ G V++ A I N K G +I I + + ++ DH
Sbjct: 249 SSNII--GNVLIDPSATIG---KNCKIGPNVVIGPNVTIGDGVR-LQRCAILKSSRVRDH 302
Query: 482 VFVGENTVVNAAVVGSYVYIGKNVVIGRRCVLKDCCMIEDNSVLPAETI 628
+V + V + +GS+ + V+G V+ D + S+LP ++I
Sbjct: 303 AWVKSSIVGWNSTLGSWSRLENVSVLGDDVVVNDEIYVNGGSILPHKSI 351
>SPAC8F11.03 |msh3|swi4|MutS protein homolog 3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1004
Score = 25.8 bits (54), Expect = 7.6
Identities = 39/175 (22%), Positives = 71/175 (40%), Gaps = 8/175 (4%)
Frame = +2
Query: 278 SRQTVLCGSQNIVLHGKVI--VQSDAIIRGDLANVKTGRFCI--ISKGSVIRPPFKKFSK 445
S + ++ GS+ I LH + + + S +II D+A F + + S PF S
Sbjct: 342 SSKCIMSGSKIIELHMEKVKSLHSLSIICLDMAISYLMEFSLEDLFVASNFYQPFDSISS 401
Query: 446 GVAFFPLQMGDHVFVGENTVVNAAVVGSYVYIGKNVV--IGRRCVLK--DCCMIEDNSVL 613
V G +FV + + VGS ++ G+R + + +++ +++
Sbjct: 402 MVLSKQALEGLELFVNQT---DHTPVGSLFWVLDRTYTRFGQRMLQRWLQKPLVDKENII 458
Query: 614 PAETIVPSFARYSGSPAHLITTLPEAMPDLMTEFTKSYYQHFLPTTA*G*IEHFY 778
V A S S I + +PDL ++ YYQ P+ ++ FY
Sbjct: 459 ERLDAVEELAFNSNSQVQAIRKMLYRLPDLEKGLSRIYYQRCTPSEMFHVLKGFY 513
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,269,527
Number of Sequences: 5004
Number of extensions: 67892
Number of successful extensions: 174
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 166
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 412451140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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