Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P07_pT_K08
(799 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 107 2e-24
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 97 3e-21
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 85 1e-17
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 82 1e-16
SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomy... 77 4e-15
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 74 2e-14
SPCC1281.04 |||pyridoxal reductase |Schizosaccharomyces pombe|ch... 35 0.016
SPCC737.06c |||glutamate-cysteine ligase regulatory subunit |Sch... 32 0.083
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 29 0.58
SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak... 27 2.4
SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Ma... 27 4.1
SPCC895.04c |ufe1||SNARE Ufe1|Schizosaccharomyces pombe|chr 3|||... 26 5.4
SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 26 7.2
SPBC1718.07c |zfs1|moc4|transcription factor Zfs1 |Schizosacchar... 25 9.5
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 107 bits (257), Expect = 2e-24
Identities = 59/167 (35%), Positives = 102/167 (61%), Gaps = 1/167 (0%)
Frame = -3
Query: 749 DYLETWQGMQDARQLGLARSIGVSNFNATQITRLV-SNSYIRPVINQIEVNPTNTQEPLV 573
D +E+W+ ++ + G R+IGVSNF I L+ S+ I P +NQIE++P +Q+ +V
Sbjct: 116 DRIESWKALEKGVEEGKLRAIGVSNFGPHHIQELLDSHPKIIPCVNQIELHPFCSQQKVV 175
Query: 572 AHCQSLGIAVMAYSPFGFVVSRGQTGAPPPRSDDPTLTALANKYRKSVGQILLRYLIDRG 393
+C+S GI + AY+P + G+ + + L A+A+KY KS QI++RY + RG
Sbjct: 176 DYCESKGIQLAAYAP----LVHGE------KFGNKQLLAIASKYNKSEAQIMIRYCLQRG 225
Query: 392 LIPIPKSTNKQRIAQNIDLFDFQLTFEEVAAINQFNKNHRVIDISDW 252
I +PKS+ +RI +N D+FDF+++ E++ + ++++ SDW
Sbjct: 226 FIVLPKSSTPRRIKENGDVFDFEISKEDMEKLYNLDEDYH----SDW 268
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 96.7 bits (230), Expect = 3e-21
Identities = 52/156 (33%), Positives = 89/156 (57%), Gaps = 1/156 (0%)
Frame = -3
Query: 743 LETWQGMQDARQLGLARSIGVSNFNATQITRL-VSNSYIRPVINQIEVNPTNTQEPLVAH 567
+ +W+ M++ G RS+GVSN+ + L SN P +NQIE++P +Q+ +V +
Sbjct: 125 IASWKAMEEFVDSGDIRSVGVSNYGVKHLQELYASNPKFYPCVNQIELHPFLSQDDIVKY 184
Query: 566 CQSLGIAVMAYSPFGFVVSRGQTGAPPPRSDDPTLTALANKYRKSVGQILLRYLIDRGLI 387
CQS IA+ AYSP + R +D L +A K SV Q+L+R+ + +G I
Sbjct: 185 CQSHDIAIEAYSPLTHGI----------RLNDEKLVPIAKKLNISVAQLLIRWSLQKGYI 234
Query: 386 PIPKSTNKQRIAQNIDLFDFQLTFEEVAAINQFNKN 279
PI KST K+ + ++D+F+F + + V ++ F+++
Sbjct: 235 PIIKSTKKEHMLSDLDVFNFTIPDDVVQELSSFDEH 270
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 85.0 bits (201), Expect = 1e-17
Identities = 60/181 (33%), Positives = 91/181 (50%), Gaps = 13/181 (7%)
Frame = -3
Query: 797 IHFPIXTK------PDDSPDNIDYL-----ETWQGMQDARQLGLARSIGVSNFNATQITR 651
IH+P+ K P D N+ Y ETW+ M+ + G R IG+SNFN T + R
Sbjct: 117 IHWPVSFKTGEDKFPKDKDGNLIYEKNPIEETWKAMEKLLETGKVRHIGLSNFNDTNLER 176
Query: 650 LVSNSYIRPVINQIEVNPTNTQEPLVAHCQSLGIAVMAYSPFGFVVSRGQTGAPPPRSDD 471
++ + ++P ++Q+E++P Q V + LGI V AYSPFG + ++ P +
Sbjct: 177 ILKVAKVKPAVHQMELHPFLPQTEFVEKHKKLGIHVTAYSPFGNQNTIYESKI-PKLIEH 235
Query: 470 PTLTALANKYRKSV--GQILLRYLIDRGLIPIPKSTNKQRIAQNIDLFDFQLTFEEVAAI 297
T+ +A + V I + + I RG IPKS N+QRI N LT E++ I
Sbjct: 236 ETIQKIAKSKGEGVTGATIAVSWAITRGTSVIPKSVNEQRIKSNFKY--IPLTKEDMDEI 293
Query: 296 N 294
N
Sbjct: 294 N 294
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 81.8 bits (193), Expect = 1e-16
Identities = 51/157 (32%), Positives = 78/157 (49%), Gaps = 2/157 (1%)
Frame = -3
Query: 740 ETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRPVINQIEVNPT--NTQEPLVAH 567
E W+ M+ A GL S+GVSNF + L+ S I P +NQIE +P +PLV
Sbjct: 123 EGWKAMETALGTGLVHSVGVSNFRIPDLEELLKTSTITPRVNQIEFHPQVYKAAKPLVEF 182
Query: 566 CQSLGIAVMAYSPFGFVVSRGQTGAPPPRSDDPTLTALANKYRKSVGQILLRYLIDRGLI 387
CQS GI V Y P +V Q P ++ +L +KY S QILL++ +G+I
Sbjct: 183 CQSKGIIVEGYGPLSPLVRDAQ----GPVAE--FTKSLESKYHVSDTQILLKWAYSKGVI 236
Query: 386 PIPKSTNKQRIAQNIDLFDFQLTFEEVAAINQFNKNH 276
PI ++ +R+ + ++ F L ++ + H
Sbjct: 237 PITTTSKIERMKECLNFDSFTLDKADIDELGTLGVQH 273
>SPBC28F2.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 276
Score = 76.6 bits (180), Expect = 4e-15
Identities = 45/141 (31%), Positives = 75/141 (53%)
Frame = -3
Query: 743 LETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRPVINQIEVNPTNTQEPLVAHC 564
+E+W+ + ++ G IGVSN+N + ++S P INQ+E + N + +++C
Sbjct: 119 IESWRALLQHQKEGRINKIGVSNYNIHHLEEIISLGLPLPAINQVEFSAFNNRPTFLSYC 178
Query: 563 QSLGIAVMAYSPFGFVVSRGQTGAPPPRSDDPTLTALANKYRKSVGQILLRYLIDRGLIP 384
+ GI V A+SP ++RG R D L L+ KY K+ ILLRY + +G+ P
Sbjct: 179 FNHGILVQAFSP----LTRGY------RLSDIRLLDLSLKYNKTPANILLRYCLQKGVSP 228
Query: 383 IPKSTNKQRIAQNIDLFDFQL 321
I K+++ I +N+ F L
Sbjct: 229 IFKASSFVHIHENVKAEQFML 249
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 74.1 bits (174), Expect = 2e-14
Identities = 48/157 (30%), Positives = 80/157 (50%), Gaps = 2/157 (1%)
Frame = -3
Query: 758 DNIDYLETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRPVINQIEVNPTNTQEP 579
+++ ETWQ M++ + G R IG+SNFN + R++ + ++P I+Q+E++P Q
Sbjct: 140 EDVPIEETWQAMEELLETGKVRYIGISNFNNEYLDRVLKIAKVKPTIHQMELHPYLPQTE 199
Query: 578 LVAHCQSLGIAVMAYSPFGFVVSRGQTGAPPPRSDDPTLTALANKYRKSV--GQILLRYL 405
+ + L I V AYSP + + TL +AN + + I + +
Sbjct: 200 YLEKHKKLQIHVSAYSPLA-NQNDAYNSDISKLIEHKTLVDIANARGEGITPANIAISWA 258
Query: 404 IDRGLIPIPKSTNKQRIAQNIDLFDFQLTFEEVAAIN 294
+ RG +PKS N+ RI N L+ LT +E+ AIN
Sbjct: 259 VKRGTSVLPKSVNESRIVSNF-LY-IPLTDKEMEAIN 293
>SPCC1281.04 |||pyridoxal reductase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 333
Score = 34.7 bits (76), Expect = 0.016
Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 3/68 (4%)
Frame = -3
Query: 455 LANKYRKSVGQILLRYLIDRG---LIPIPKSTNKQRIAQNIDLFDFQLTFEEVAAINQFN 285
LA K+ S+ + L ++I G +IPIP ST QR N+ L+ E++ +
Sbjct: 254 LAQKFGMSMPEFALNFIIANGKGMIIPIPGSTTVQRAESNLSALKKSLSSEQLEEAKKVL 313
Query: 284 KNHRVIDI 261
H++ +
Sbjct: 314 DKHQIFGL 321
>SPCC737.06c |||glutamate-cysteine ligase regulatory subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 287
Score = 32.3 bits (70), Expect = 0.083
Identities = 13/48 (27%), Positives = 28/48 (58%)
Frame = -3
Query: 746 YLETWQGMQDARQLGLARSIGVSNFNATQITRLVSNSYIRPVINQIEV 603
+++TW+ +++ G ++GVS F ++ RL+S+ + P QI +
Sbjct: 158 WVDTWKLLEEKVGEGKIGTLGVSEFGVNELQRLISSVNVVPESTQINI 205
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 29.5 bits (63), Expect = 0.58
Identities = 25/82 (30%), Positives = 39/82 (47%), Gaps = 6/82 (7%)
Frame = -3
Query: 464 LTALANKYRKSVGQILLRYLID--RGLI---PIPKSTNKQRIAQNIDLFDFQLTFEEVAA 300
L+AL KS + YL++ RG I P+P T K++ A + + F+ F +
Sbjct: 2189 LSALVQIIEKSSSYEICNYLLEIVRGWIMNSPVPVPTVKEKAALLLKMVTFEGRFSQNEQ 2248
Query: 299 INQFNKNHRVI-DISDWKDYPN 237
+ FNK I DI + + Y N
Sbjct: 2249 NDLFNKYLSFIADIYEMEPYKN 2270
>SPBC1861.03 |mak10||NatC N-acetyltransferase complex subunit Mak10
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 708
Score = 27.5 bits (58), Expect = 2.4
Identities = 16/38 (42%), Positives = 22/38 (57%), Gaps = 4/38 (10%)
Frame = +3
Query: 594 CRIHLNLVNNRPDVTVAD---QSRDLRSVEVGY-SNGP 695
CR NL+N P++TVA ++ DL+ V SNGP
Sbjct: 443 CRFRRNLINLLPEITVAHFEAEALDLKFVAKSLPSNGP 480
>SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr
2|||Manual
Length = 918
Score = 26.6 bits (56), Expect = 4.1
Identities = 13/28 (46%), Positives = 18/28 (64%), Gaps = 1/28 (3%)
Frame = +1
Query: 100 SFNQKILERNKKSSKL-IFLIYFNFIHI 180
SFNQK++E K KL F I+ F+H+
Sbjct: 505 SFNQKLMEYFKGFVKLDYFSIFITFLHV 532
>SPCC895.04c |ufe1||SNARE Ufe1|Schizosaccharomyces pombe|chr
3|||Manual
Length = 319
Score = 26.2 bits (55), Expect = 5.4
Identities = 14/61 (22%), Positives = 26/61 (42%)
Frame = +3
Query: 276 MILVKLVNGRYLFKRQLEIE*IDVLSDPLLVCGFRNRD*TSVDQISE*NLSNTLPVFISE 455
++ +K + G YL R + LS PL+ C +D++ + + + IS
Sbjct: 48 LVFLKKIRGAYLKDRTFSN--VQRLSKPLMECSLEELSKLELDEVQRDEIEHEVSSAISS 105
Query: 456 C 458
C
Sbjct: 106 C 106
>SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor
Thi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 25.8 bits (54), Expect = 7.2
Identities = 24/87 (27%), Positives = 41/87 (47%), Gaps = 3/87 (3%)
Frame = -3
Query: 689 IGVSNFNATQI-TRLVSNSY-IRP-VINQIEVNPTNTQEPLVAHCQSLGIAVMAYSPFGF 519
+ SNF+A+ I RL S + I P V+ Q +V+ + + + QS +++ FG
Sbjct: 309 VSKSNFHASNIGNRLFSAALSIFPLVLLQSDVSAVQSSLLIGLYLQSTIYEKSSFAYFGL 368
Query: 518 VVSRGQTGAPPPRSDDPTLTALANKYR 438
+ SDDP+LT + + R
Sbjct: 369 AIKFAVALGLHKNSDDPSLTQNSKELR 395
>SPBC1718.07c |zfs1|moc4|transcription factor Zfs1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 404
Score = 25.4 bits (53), Expect = 9.5
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +2
Query: 500 SDHATPRTRTDCTPSRRYRDSGSVPLAALVCL*DSPQS 613
S+H +P + + PSR SGS+ + L L D +S
Sbjct: 147 SNHPSPISSLESLPSRSSTGSGSLDFSGLANLHDDSKS 184
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,291,965
Number of Sequences: 5004
Number of extensions: 67812
Number of successful extensions: 198
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 389395636
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -