Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= fcaL-P07_pT_J03
         (841 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po...    83   5e-17
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ...    80   4e-16
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po...    67   3e-12
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac...    38   0.002
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ...    36   0.009
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc...    33   0.067
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha...    31   0.20 
SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces po...    29   1.1  
SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase Cmk2|Schiz...    28   1.9  
SPAC1687.15 |gsk3|skp1|serine/threonine protein kinase Gsk3|Schi...    27   2.5  
SPAC1006.03c |||human CCDC131 homolog|Schizosaccharomyces pombe|...    27   3.3  
SPCC4G3.08 |psk1||serine/threonine protein kinase Psk1|Schizosac...    26   5.8  
SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase Ubp8|Schizos...    26   7.6  

>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 492

 Score = 83.0 bits (196), Expect = 5e-17
 Identities = 40/87 (45%), Positives = 53/87 (60%), Gaps = 1/87 (1%)
 Frame = -2

Query: 819 YAPWCGHCQKLAPVWEELGEKLKDE-EVDIIKIDATANDWPKSQFDVSGFPTIFWKPKDS 643
           YAPWCGHC+ LAP +E+L E+  D+  V + KIDAT ND   S   +SGFPTI +   + 
Sbjct: 381 YAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDATENDISVS---ISGFPTIMFFKAND 437

Query: 642 SKKPQRYNGGRALEDFIKYVSEQATSE 562
              P RY G R LED   ++ + A+ E
Sbjct: 438 KVNPVRYEGDRTLEDLSAFIDKHASFE 464



 Score = 66.5 bits (155), Expect = 4e-12
 Identities = 27/90 (30%), Positives = 53/90 (58%)
 Frame = -2

Query: 825 RXYAPWCGHCQKLAPVWEELGEKLKDEEVDIIKIDATANDWPKSQFDVSGFPTIFWKPKD 646
           + YAPWCGHC+ LAP +E   ++L+ + + ++++D T      S++ + G+PT+      
Sbjct: 45  KFYAPWCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRGYPTL--NVFK 102

Query: 645 SSKKPQRYNGGRALEDFIKYVSEQATSELK 556
           + K+  +Y+G R  +  +KY+ +Q    +K
Sbjct: 103 NGKQISQYSGPRKHDALVKYMRKQLLPTVK 132


>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 359

 Score = 79.8 bits (188), Expect = 4e-16
 Identities = 36/87 (41%), Positives = 58/87 (66%), Gaps = 1/87 (1%)
 Frame = -2

Query: 819 YAPWCGHCQKLAPVWEELGEKLKDE-EVDIIKIDATANDWPKSQFDVSGFPTIFWKPKDS 643
           YA WCG+C++LAP +E LG+  K+E  V+I+KI+A          +V+ FPTI + PKD 
Sbjct: 166 YADWCGYCKRLAPTYETLGKVFKNEPNVEIVKINADVFADIGRLHEVASFPTIKFFPKDD 225

Query: 642 SKKPQRYNGGRALEDFIKYVSEQATSE 562
             KP+ Y G R+LE  I+Y+++++ ++
Sbjct: 226 KDKPELYEGDRSLESLIEYINKKSGTQ 252



 Score = 79.4 bits (187), Expect = 6e-16
 Identities = 37/90 (41%), Positives = 56/90 (62%), Gaps = 1/90 (1%)
 Frame = -2

Query: 840 QGRTHRXYAPWCGHCQKLAPVWEELGEKLKD-EEVDIIKIDATANDWPKSQFDVSGFPTI 664
           +G     YA WCGHC+ LAPV+EELG   +D  +V I KIDA  +     ++ ++GFPT+
Sbjct: 40  KGALIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGKIDADTHSDVADKYHITGFPTL 99

Query: 663 FWKPKDSSKKPQRYNGGRALEDFIKYVSEQ 574
            W P D S +P +Y+  R ++   ++VSE+
Sbjct: 100 IWFPPDGS-EPVQYSNARDVDSLTQFVSEK 128


>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 363

 Score = 67.3 bits (157), Expect = 3e-12
 Identities = 36/92 (39%), Positives = 50/92 (54%), Gaps = 4/92 (4%)
 Frame = -2

Query: 819 YAPWCGHCQKLAPVWEELGEKLKD-EEVDIIKIDATANDWPKSQFDVSGFPTI---FWKP 652
           YAPWCG+C+KL P +++L   L     V  +  DA  N    SQ+ V GFPTI   +   
Sbjct: 56  YAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSS 115

Query: 651 KDSSKKPQRYNGGRALEDFIKYVSEQATSELK 556
           K SS     YNG R+ +   K+VS+   S++K
Sbjct: 116 KGSSLSSTDYNGDRSYKSLQKFVSDSIPSKVK 147


>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
           Txl1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 290

 Score = 37.5 bits (83), Expect = 0.002
 Identities = 21/86 (24%), Positives = 40/86 (46%), Gaps = 2/86 (2%)
 Frame = -2

Query: 819 YAPWCGHCQKLAPVWEELGEKLKDEEVDIIKIDATANDWPKSQFDVSGFPTIFWKPKDSS 640
           YA WCG C+ ++P++ +L  K    +    K++        S   V   PT  +   ++ 
Sbjct: 27  YADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVF--FENG 84

Query: 639 KKPQRYNGG--RALEDFIKYVSEQAT 568
           K+     G   +AL++ +  +S +AT
Sbjct: 85  KQIDMLTGANPQALKEKVALISSKAT 110


>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 726

 Score = 35.5 bits (78), Expect = 0.009
 Identities = 11/27 (40%), Positives = 19/27 (70%)
 Frame = -2

Query: 825 RXYAPWCGHCQKLAPVWEELGEKLKDE 745
           + Y P CG C++L P+W+ + EK K++
Sbjct: 48  KYYLPSCGACKRLGPMWDNMVEKAKEQ 74


>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 103

 Score = 32.7 bits (71), Expect = 0.067
 Identities = 15/53 (28%), Positives = 24/53 (45%)
 Frame = -2

Query: 819 YAPWCGHCQKLAPVWEELGEKLKDEEVDIIKIDATANDWPKSQFDVSGFPTIF 661
           +A WCG C+ +AP +E+      D     IK+D        ++  V   P+ F
Sbjct: 26  FATWCGPCKAIAPKFEQFSNTYSD--ATFIKVDVDQLSEIAAEAGVHAMPSFF 76


>SPBC12D12.07c |trx2||mitochondrial thioredoxin
           Trx2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 121

 Score = 31.1 bits (67), Expect = 0.20
 Identities = 13/34 (38%), Positives = 21/34 (61%)
 Frame = -2

Query: 819 YAPWCGHCQKLAPVWEELGEKLKDEEVDIIKIDA 718
           YA WCG C+ L P  E+L E  ++++   I ++A
Sbjct: 43  YADWCGPCKYLKPFLEKLSE--QNQKASFIAVNA 74


>SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1313

 Score = 28.7 bits (61), Expect = 1.1
 Identities = 12/42 (28%), Positives = 19/42 (45%)
 Frame = +2

Query: 326 LQEERQCLSTQRMLFISAFPWSDITHINSEVIFHCFSHYIHP 451
           + E R   +    +F   FP   +  +N +V+ HC SH   P
Sbjct: 592 ISEHRAMCAFILSVFCRGFPQGQLACLNPQVLSHCLSHLNSP 633


>SPAC23A1.06c |cmk2|mkp2|MAPK-activated protein kinase
           Cmk2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 504

 Score = 27.9 bits (59), Expect = 1.9
 Identities = 15/61 (24%), Positives = 30/61 (49%)
 Frame = -2

Query: 804 GHCQKLAPVWEELGEKLKDEEVDIIKIDATANDWPKSQFDVSGFPTIFWKPKDSSKKPQR 625
           G  + L+P W+++ +  KD    ++ +D      P+ ++D+  F    W  K  SK P+ 
Sbjct: 308 GEFEFLSPWWDDISDSAKDLITHLLTVD------PRERYDIHQFFQHPW-IKGESKMPEN 360

Query: 624 Y 622
           +
Sbjct: 361 F 361


>SPAC1687.15 |gsk3|skp1|serine/threonine protein kinase
           Gsk3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 387

 Score = 27.5 bits (58), Expect = 2.5
 Identities = 13/35 (37%), Positives = 20/35 (57%), Gaps = 3/35 (8%)
 Frame = +1

Query: 58  RTSQLM*LIYHPQVE---VYQYTTYDNTSDFYKNV 153
           R  Q+M ++ HP +     Y YTT DN+ + Y N+
Sbjct: 72  RELQIMRIMKHPNIVDLIAYYYTTGDNSDEVYLNL 106


>SPAC1006.03c |||human CCDC131 homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 714

 Score = 27.1 bits (57), Expect = 3.3
 Identities = 11/29 (37%), Positives = 18/29 (62%)
 Frame = -2

Query: 816 APWCGHCQKLAPVWEELGEKLKDEEVDII 730
           APW    +++A V EE   ++K  E+DI+
Sbjct: 453 APWLRKTEQIAVVHEEHPAQIKKSEIDIL 481


>SPCC4G3.08 |psk1||serine/threonine protein kinase
           Psk1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 436

 Score = 26.2 bits (55), Expect = 5.8
 Identities = 16/45 (35%), Positives = 25/45 (55%)
 Frame = -3

Query: 332 LAVVMLHSLTIIFTDNRSRITLLDLQNNTDA*TQFHLSLLSVYGA 198
           LA++ LH L I++ D +    LLD + +    T F LS ++  GA
Sbjct: 201 LALIHLHKLGIVYRDLKPENCLLDAEGHI-LLTDFGLSKVAENGA 244


>SPAC13A11.04c |ubp8||ubiquitin C-terminal hydrolase
           Ubp8|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 449

 Score = 25.8 bits (54), Expect = 7.6
 Identities = 14/41 (34%), Positives = 21/41 (51%)
 Frame = -2

Query: 660 WKPKDSSKKPQRYNGGRALEDFIKYVSEQATSELKGWDRKG 538
           W+  D  + P++YN    LE + KY    AT+ L+G    G
Sbjct: 112 WQ-SDHKRLPEKYNQMVCLEAYRKYPPVCATAGLRGIQNLG 151


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,491,553
Number of Sequences: 5004
Number of extensions: 75622
Number of successful extensions: 272
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 235
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 266
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 414453330
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)