Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P07_pT_F18
(752 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 35 0.014
SPBC359.04c |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Ma... 33 0.058
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 33 0.058
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 31 0.23
SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar... 30 0.41
SPBC1D7.02c |scr1||transcription factor Scr1|Schizosaccharomyces... 29 0.54
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 29 0.71
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 29 0.94
SPBC800.10c |||EPS15 repeat family actin cortical patch componen... 27 2.2
SPBPB2B2.09c |||2-dehydropantoate 2-reductase |Schizosaccharomyc... 27 2.9
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 26 5.0
SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase Shk1|Schizosacc... 26 6.6
SPMIT.02 |||mitochondrial DNA binding endonuclease|Schizosacchar... 26 6.6
SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1 |Schizosacch... 26 6.6
SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha... 26 6.6
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 26 6.6
SPAP27G11.15 |slx1||structure-specific endonuclease catalytic su... 26 6.6
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ... 26 6.6
SPAC1039.09 |isp5||amino acid permease Isp5|Schizosaccharomyces ... 25 8.8
SPCC18.05c |||notchless-like protein|Schizosaccharomyces pombe|c... 25 8.8
SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 25 8.8
SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb... 25 8.8
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 34.7 bits (76), Expect = 0.014
Identities = 47/239 (19%), Positives = 94/239 (39%), Gaps = 3/239 (1%)
Frame = -3
Query: 711 LQAPNSSAPXKTASMKTTGSAISSSNVLMESPQPSSARTVS-CSTLLSGRXXXXXXXXXX 535
+ + +SS A T+ S +SSS+V+ S PSS+ + + S+ LS
Sbjct: 189 VSSTSSSTFSSAAPTSTSSSYLSSSSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTSSSSS 248
Query: 534 XXAIGLSSSPPSLTHNVPAAMDSLPIPTPLCATSSTTAXXXXXXXXXXXPAFTSTSTPVP 355
+ LSSS S T + ++ S+ + ++S T+ + TSTS+ +
Sbjct: 249 STSSSLSSSSSSSTASSSSSSSSIISSSSSSSSSPTSTSSTISSSSSSSSSPTSTSSTIS 308
Query: 354 ASGPIPPEDKDVTNNKKKQRTA--SSAPKSSWLMPKDRXXXXXXXXXXXTANVSTYA*TV 181
+S ++ ++ SS+P SS T + S + +
Sbjct: 309 SSSSSSSSFSSTLSSSSMSSSSSFSSSPTSSSSTISSSSSSPSSSSFSSTTSSSKSSSSF 368
Query: 180 SSPVTSAAKSERFTMRRARNAMRLRTFADARTGTRTLRKPHQDPNRDLKPYSDTTLYYN 4
SS V+S++ + T+ + ++ + + + + + K P S + Y+N
Sbjct: 369 SSTVSSSSSTSSSTLTSSSSSSSRPASSSSHSSSLSSHKSSSSSKSSSAPVS-SAFYHN 426
>SPBC359.04c |||DIPSY family|Schizosaccharomyces pombe|chr
2|||Manual
Length = 358
Score = 32.7 bits (71), Expect = 0.058
Identities = 16/42 (38%), Positives = 28/42 (66%)
Frame = -3
Query: 696 SSAPXKTASMKTTGSAISSSNVLMESPQPSSARTVSCSTLLS 571
SS+ TAS+ T+ S +S+S + SP P+++ T + S++LS
Sbjct: 94 SSSTPITASVPTSSSILSNSTIPTTSPVPTTSSTPTSSSILS 135
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 32.7 bits (71), Expect = 0.058
Identities = 16/38 (42%), Positives = 27/38 (71%), Gaps = 1/38 (2%)
Frame = -3
Query: 678 TASMKTTGSAISSSNVLMESPQPS-SARTVSCSTLLSG 568
T+S + S+ISSS+ ++ SP PS S+ +S S+++SG
Sbjct: 608 TSSPSSISSSISSSSTILSSPTPSTSSLMISSSSIISG 645
Score = 32.3 bits (70), Expect = 0.076
Identities = 34/141 (24%), Positives = 58/141 (41%)
Frame = -3
Query: 702 PNSSAPXKTASMKTTGSAISSSNVLMESPQPSSARTVSCSTLLSGRXXXXXXXXXXXXAI 523
P SS+ ++S+ T S ISSS++ SS + S S+ S +
Sbjct: 546 PTSSSSDFSSSITTISSGISSSSIPSTFSSVSSILSSSTSSPSSTSLSISSSSTSSTFSS 605
Query: 522 GLSSSPPSLTHNVPAAMDSLPIPTPLCATSSTTAXXXXXXXXXXXPAFTSTSTPVPASGP 343
+SSP S++ ++ ++ L PTP +TSS +S ST +P S
Sbjct: 606 ASTSSPSSISSSISSSSTILSSPTP--STSSLMISSSSIISGSSSILSSSIST-IPISSS 662
Query: 342 IPPEDKDVTNNKKKQRTASSA 280
+ V + ++SS+
Sbjct: 663 LSTYSSSVIPSSSTLVSSSSS 683
Score = 26.2 bits (55), Expect = 5.0
Identities = 33/142 (23%), Positives = 55/142 (38%), Gaps = 7/142 (4%)
Frame = -3
Query: 672 SMKTTGSAISSSNVLMES-----PQPSSAR--TVSCSTLLSGRXXXXXXXXXXXXAIGLS 514
S T SA S+++++ S QP+S R T + S++ + ++ S
Sbjct: 414 STSTLISASDSTSIIVSSYVSTVTQPASTRVQTTTVSSISTSVKQPTASVASSSVSVPSS 473
Query: 513 SSPPSLTHNVPAAMDSLPIPTPLCATSSTTAXXXXXXXXXXXPAFTSTSTPVPASGPIPP 334
SS + ++ S P +TSS A STS+ P+S I
Sbjct: 474 SSVQPQSSTPISSSSSASSPQSTLSTSSEVVSEVSSTLLSGSSAIPSTSSSTPSSSIISS 533
Query: 333 EDKDVTNNKKKQRTASSAPKSS 268
V ++ T+SS+ SS
Sbjct: 534 PMTSVLSSSSSIPTSSSSDFSS 555
Score = 25.4 bits (53), Expect = 8.8
Identities = 19/48 (39%), Positives = 26/48 (54%)
Frame = -3
Query: 720 SXQLQAPNSSAPXKTASMKTTGSAISSSNVLMESPQPSSARTVSCSTL 577
S L A +SS+ T+ +T SSSN L+ S SSA VS +T+
Sbjct: 720 SLSLTAMSSSSAIPTSVNSSTLITASSSNTLLSSITSSSA-IVSSTTV 766
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 30.7 bits (66), Expect = 0.23
Identities = 36/147 (24%), Positives = 60/147 (40%), Gaps = 1/147 (0%)
Frame = -3
Query: 705 APNSSAPXKTASMKTTGSAISSSNVLMESPQPSSARTVSCSTLLSGRXXXXXXXXXXXXA 526
A +SS AS T S+++SS++ + +++ ++S ST+ S +
Sbjct: 208 ATSSSLSSTAASNSATSSSLASSSLNSTTSATATSSSIS-STVSSSTPLTSSNSTTAATS 266
Query: 525 IGLSSSPPSLTHNVPAAM-DSLPIPTPLCATSSTTAXXXXXXXXXXXPAFTSTSTPVPAS 349
+SS S +N + + S P TPL + +STTA TS ++ S
Sbjct: 267 ASATSS--SAQYNTSSLLPSSTPSSTPLSSANSTTA------TSASSTPLTSVNSTTTTS 318
Query: 348 GPIPPEDKDVTNNKKKQRTASSAPKSS 268
P + N + SS P SS
Sbjct: 319 ASSTPLSSVSSANSTTATSTSSTPLSS 345
Score = 30.7 bits (66), Expect = 0.23
Identities = 26/118 (22%), Positives = 48/118 (40%)
Frame = -3
Query: 720 SXQLQAPNSSAPXKTASMKTTGSAISSSNVLMESPQPSSARTVSCSTLLSGRXXXXXXXX 541
S L + NS+ +AS ++ + ++S++L S S+ + + ST +
Sbjct: 252 STPLTSSNSTTAATSASATSSSAQYNTSSLLPSSTPSSTPLSSANSTTATSASSTPLTSV 311
Query: 540 XXXXAIGLSSSPPSLTHNVPAAMDSLPIPTPLCATSSTTAXXXXXXXXXXXPAFTSTS 367
SS+P S + + + TPL + +STTA + T+TS
Sbjct: 312 NSTTTTSASSTPLSSVSSANSTTATSTSSTPLSSVNSTTATSASSTPLTSVNSTTATS 369
>SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 988
Score = 29.9 bits (64), Expect = 0.41
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = -3
Query: 375 STSTPVPASGPIPPEDKDVTNNKKKQRTASSAPK 274
S+STP PAS PP ++++K R+ +P+
Sbjct: 619 SSSTPTPASVLAPPSSASLSSSKDANRSVPESPR 652
>SPBC1D7.02c |scr1||transcription factor Scr1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 565
Score = 29.5 bits (63), Expect = 0.54
Identities = 20/44 (45%), Positives = 23/44 (52%)
Frame = -3
Query: 699 NSSAPXKTASMKTTGSAISSSNVLMESPQPSSARTVSCSTLLSG 568
NSS P K AS G SSN S + SS+ VS S LL+G
Sbjct: 490 NSSMPHKPASQSNVGPVRISSN--RRSRKFSSSSRVSVSNLLAG 531
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 29.1 bits (62), Expect = 0.71
Identities = 36/131 (27%), Positives = 47/131 (35%), Gaps = 7/131 (5%)
Frame = -3
Query: 522 GLSSSPPSLTHNVP-AAMDSLPIPTPLCATSSTTAXXXXXXXXXXXPAFTSTSTP----- 361
G SS +T VP + S IP P +TSST TSTS P
Sbjct: 239 GSSSLSTPITPTVPPTSTSSTSIPIPPTSTSSTDTNSSPLPTTSTSCT-TSTSIPPTGNS 297
Query: 360 -VPASGPIPPEDKDVTNNKKKQRTASSAPKSSWLMPKDRXXXXXXXXXXXTANVSTYA*T 184
P + +PP T+ + SS SS +P T N ST T
Sbjct: 298 TTPVTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGN-STTPVT 356
Query: 183 VSSPVTSAAKS 151
+ P TS + +
Sbjct: 357 PTVPPTSTSST 367
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 28.7 bits (61), Expect = 0.94
Identities = 32/151 (21%), Positives = 60/151 (39%)
Frame = -3
Query: 720 SXQLQAPNSSAPXKTASMKTTGSAISSSNVLMESPQPSSARTVSCSTLLSGRXXXXXXXX 541
S + +SS+P +++ TT + SSS+ S SS+ + S S+ S
Sbjct: 133 SSTTSSSSSSSPSSSSTTTTTSPSSSSSS---SSSSSSSSSSSSSSSSSSSSSSSSSSSS 189
Query: 540 XXXXAIGLSSSPPSLTHNVPAAMDSLPIPTPLCATSSTTAXXXXXXXXXXXPAFTSTSTP 361
+ SSS S + +VP + + ++SS+++ S+ST
Sbjct: 190 SSSSSSSSSSSSSSSSSSVPITSSTSSSHSSSSSSSSSSSSSSRPSSSSSFITTMSSSTF 249
Query: 360 VPASGPIPPEDKDVTNNKKKQRTASSAPKSS 268
+ P T+++ TA+ A +S
Sbjct: 250 ISTVTVTPSSSSSSTSSEVPSSTAALALNAS 280
>SPBC800.10c |||EPS15 repeat family actin cortical patch component
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1116
Score = 27.5 bits (58), Expect = 2.2
Identities = 23/102 (22%), Positives = 36/102 (35%)
Frame = -3
Query: 627 MESPQPSSARTVSCSTLLSGRXXXXXXXXXXXXAIGLSSSPPSLTHNVPAAMDSLPIPTP 448
ME PQ + V ST S R +++ P + + +++ PTP
Sbjct: 835 MEEPQHQDSSVVDVSTSASQRGSPVLSDLSKLTGSARNTAEP-VENTSAEPIENTSAPTP 893
Query: 447 LCATSSTTAXXXXXXXXXXXPAFTSTSTPVPASGPIPPEDKD 322
+ A A + STP P P+PP +D
Sbjct: 894 FEIANKQQATEPISAPF----ATETISTPAPVKPPVPPSRRD 931
>SPBPB2B2.09c |||2-dehydropantoate 2-reductase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 350
Score = 27.1 bits (57), Expect = 2.9
Identities = 21/61 (34%), Positives = 30/61 (49%), Gaps = 9/61 (14%)
Frame = -2
Query: 217 NDCQRFYV--CLNGVEPRDLGCQVGEVYNEESQK----CDAPENVR---GCEDWYKDSEE 65
N C++ + C+N L C GE+YN+ES K C E V C +K++EE
Sbjct: 209 NQCEKLVINACINPTTAT-LDCVNGELYNDESAKELFRCIIKECVDIFFKCIPLFKNNEE 267
Query: 64 A 62
A
Sbjct: 268 A 268
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 26.2 bits (55), Expect = 5.0
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = -2
Query: 670 YEDDRQCDKFFECVDGVATTKLCPDGLVFDPTIRK 566
Y DD ++ E V G T +C ++FD TIR+
Sbjct: 494 YLDDFPLEEIDEHVLGSTITLVCQQPVIFDMTIRE 528
>SPBC1604.14c |shk1|pak1, orb2|PAK-related kinase
Shk1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 658
Score = 25.8 bits (54), Expect = 6.6
Identities = 36/164 (21%), Positives = 59/164 (35%), Gaps = 5/164 (3%)
Frame = -3
Query: 726 RQSXQLQAPNSSAPXKTASMKTTGSAISSSNVLMESPQPSSARTVSCSTLLSGRXXXXXX 547
R+S + + SA ++ ++ T + SSS + P P+ + S S LLS +
Sbjct: 220 RESTEKPLLSVSALSSSSHLQPTSATSSSSRLYPSRPAPTPPASSSSSPLLSSQTVKTTT 279
Query: 546 XXXXXXAIGLSSSPPSLTHNVPAAMDSLPIPTPLCATSSTTAXXXXXXXXXXXPAFTST- 370
L SS T N+ + + + +TS T A TST
Sbjct: 280 SNASRQPSPLVSS--KSTDNIIRSHSPVLLTPQTLSTSETKHIRPNNSTPYQRRAETSTK 337
Query: 369 ----STPVPASGPIPPEDKDVTNNKKKQRTASSAPKSSWLMPKD 250
+TP P P + ++ +A A S PK+
Sbjct: 338 PKAVATPQKVEAPSAPRLQKRAPRQQSNDSAVLAKLQSICNPKN 381
>SPMIT.02 |||mitochondrial DNA binding
endonuclease|Schizosaccharomyces pombe|chr
mitochondrial|||Manual
Length = 384
Score = 25.8 bits (54), Expect = 6.6
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +2
Query: 260 INQLLFGALEAVLCFFLLFVTSLSSG 337
I LE +LCFFL++ T+ S G
Sbjct: 47 IRDSFLSQLENILCFFLVYRTTYSFG 72
>SPCC895.09c |ucp12||ATP-dependent RNA helicase Ucp1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1327
Score = 25.8 bits (54), Expect = 6.6
Identities = 10/31 (32%), Positives = 18/31 (58%)
Frame = -3
Query: 342 IPPEDKDVTNNKKKQRTASSAPKSSWLMPKD 250
+PPE +++ + +KQ+ K SWL +D
Sbjct: 162 LPPEHRNIWFDMEKQKKEELKNKHSWLYNED 192
>SPAPJ696.01c |vps17||retromer complex subunit
Vps17|Schizosaccharomyces pombe|chr 1|||Manual
Length = 549
Score = 25.8 bits (54), Expect = 6.6
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = -3
Query: 675 ASMKTTGSAISSSNVLMESPQPSSART 595
+S T S +S++N L +SP+ S ART
Sbjct: 356 SSKNQTNSYLSAANRLQDSPKISKART 382
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 25.8 bits (54), Expect = 6.6
Identities = 18/77 (23%), Positives = 27/77 (35%)
Frame = -3
Query: 486 VPAAMDSLPIPTPLCATSSTTAXXXXXXXXXXXPAFTSTSTPVPASGPIPPEDKDVTNNK 307
VP+ +PIPT +++ P+ S P P+ P P +
Sbjct: 1036 VPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVP 1095
Query: 306 KKQRTASSAPKSSWLMP 256
K A PK S +P
Sbjct: 1096 KPSVAAPPVPKPSVAVP 1112
>SPAP27G11.15 |slx1||structure-specific endonuclease catalytic
subunit |Schizosaccharomyces pombe|chr 1|||Manual
Length = 271
Score = 25.8 bits (54), Expect = 6.6
Identities = 8/21 (38%), Positives = 11/21 (52%)
Frame = -2
Query: 454 DPSVCNIFYNCIEGEATEVKC 392
+P CN+ Y CIE + C
Sbjct: 176 EPVKCNLCYECIESDELRANC 196
>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
2|||Manual
Length = 962
Score = 25.8 bits (54), Expect = 6.6
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = +3
Query: 69 SESLYQSSHPRTFSGASHFWLSSL*TSPTWQPRSR 173
++ L +S HP + SG S+ +++S WQ R R
Sbjct: 728 NDKLIKSIHPGSLSGISNSFVNSKHPIEQWQSRLR 762
>SPAC1039.09 |isp5||amino acid permease Isp5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 580
Score = 25.4 bits (53), Expect = 8.8
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = +2
Query: 257 GINQLLFGALEAVLCFFLLFVTSLSSGGIGPDAG 358
GI +GA +L F F S+ G PDAG
Sbjct: 480 GIYGACYGAFIIILVFITEFYVSIFPIGASPDAG 513
>SPCC18.05c |||notchless-like protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 502
Score = 25.4 bits (53), Expect = 8.8
Identities = 12/48 (25%), Positives = 21/48 (43%)
Frame = +3
Query: 573 IVGSNTRPSGQSLVVATPSTHSKNLSHCLSSSYWPSLXGHLNWALAIA 716
I+ + PS S +V + L C + + ++ GH NW +A
Sbjct: 137 IISAQFSPSTSSRLVTGSGDFTARLWDCDTQTPIATMKGHTNWVSCVA 184
>SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 3971
Score = 25.4 bits (53), Expect = 8.8
Identities = 15/44 (34%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
Frame = -3
Query: 699 NSSAPXKTASMKTTGSAISSSNVLMESPQPSSARTVS-CSTLLS 571
NSS P ++S+ + + ISSS V+ S S+ +S S+++S
Sbjct: 3463 NSSTPITSSSVLNSSTPISSSTVITSSVVIGSSSVLSYASSIVS 3506
>SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 368
Score = 25.4 bits (53), Expect = 8.8
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = -3
Query: 378 TSTSTPVPASGPIPPEDKDVTNNKKKQRTASSAPKSS 268
TSTSTP A+ P P V K + +++ A +S
Sbjct: 77 TSTSTPKSAASPAPNPPASVPEKKVEAPSSTVAESTS 113
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,098,903
Number of Sequences: 5004
Number of extensions: 66491
Number of successful extensions: 307
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 257
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 298
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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