Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P07_F_J21
(730 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 40 4e-04
SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3 Rfp1|Schizo... 39 6e-04
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 39 6e-04
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 36 0.006
SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomy... 36 0.008
SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr 1|||... 33 0.042
SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch... 32 0.073
SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb... 31 0.22
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 30 0.29
SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi... 29 0.68
SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3 Brl2|Schizosa... 29 0.90
SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 29 0.90
SPCC1494.04c |tyr1||prephenate dehydrogenase [NADP+] |Schizosacc... 28 1.6
SPAC343.03 |apc11||anaphase-promoting complex subunit Apc11|Schi... 28 1.6
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 28 1.6
SPBC776.18c |pmh1|mcr1|transcription factor TFIIH complex subuni... 27 3.6
SPBC216.06c |swi1||replication fork protection complex subunit S... 26 4.8
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 26 4.8
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 26 6.3
SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p... 25 8.4
SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 25 8.4
SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar... 25 8.4
SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c... 25 8.4
>SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 673
Score = 39.9 bits (89), Expect = 4e-04
Identities = 18/45 (40%), Positives = 26/45 (57%), Gaps = 2/45 (4%)
Frame = +2
Query: 236 IIESSIKFGDCPICCEELGTNPLAS--TKCGHVYCLKCLERCLKT 364
+ S + CP C EE P+A+ ++CGHVYC CL R ++T
Sbjct: 204 LCSSDFQLAACPFCLEE---KPVAARMSRCGHVYCFSCLLRFVET 245
>SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3
Rfp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 254
Score = 39.1 bits (87), Expect = 6e-04
Identities = 21/42 (50%), Positives = 25/42 (59%), Gaps = 6/42 (14%)
Frame = +2
Query: 266 CPICCEELGTNP------LASTKCGHVYCLKCLERCLKTEKK 373
CP C E LGT+ L +TKCGHVYC C + LKT K+
Sbjct: 189 CPRCQEPLGTSKSKEKSALWATKCGHVYCGSC-AKVLKTSKR 229
>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 468
Score = 39.1 bits (87), Expect = 6e-04
Identities = 18/36 (50%), Positives = 21/36 (58%)
Frame = +2
Query: 263 DCPICCEELGTNPLASTKCGHVYCLKCLERCLKTEK 370
+CPIC E L P +T CGH YC +CL LK K
Sbjct: 84 ECPICTEAL-QRPF-TTHCGHTYCYECLLNWLKESK 117
>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1133
Score = 35.9 bits (79), Expect = 0.006
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +2
Query: 263 DCPICCEELGTNPLASTKCGHVYCLKCLERCLKTEKKMSYL 385
+CPICC E NPL C H C CL ++ +K+ + +
Sbjct: 876 ECPICCNEPIQNPLL-LNCKHACCGDCLSEHIQYQKRRNII 915
>SPAC1002.14 |itt1||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 435
Score = 35.5 bits (78), Expect = 0.008
Identities = 15/38 (39%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Frame = +2
Query: 236 IIESSIKFGDCPICCEEL-GTNPLASTKCGHVYCLKCL 346
++E I+ C +C +E GT+ T+CGHV C CL
Sbjct: 164 LLEFQIRKFQCNVCFDEFNGTDCFQLTRCGHVSCQSCL 201
>SPAC328.02 |||Ariadne homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 504
Score = 33.1 bits (72), Expect = 0.042
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +2
Query: 260 GDCPICCEELGTNPLASTKCGHVYCLKCLERCL 358
G C IC +E G P S +C H +CL C + L
Sbjct: 129 GTCEICYDE-GCLPFFSAECDHEFCLACYRQYL 160
>SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 269
Score = 32.3 bits (70), Expect = 0.073
Identities = 16/48 (33%), Positives = 25/48 (52%), Gaps = 2/48 (4%)
Frame = +2
Query: 227 RKPIIESSIKFGD--CPICCEELGTNPLASTKCGHVYCLKCLERCLKT 364
+K ++ SS + D C IC + L+ T CGH++C C+ L T
Sbjct: 191 KKQVVPSSQRLADYKCVICLDS--PENLSCTPCGHIFCNFCILSALGT 236
>SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 741
Score = 30.7 bits (66), Expect = 0.22
Identities = 14/47 (29%), Positives = 26/47 (55%), Gaps = 3/47 (6%)
Frame = +2
Query: 230 KPIIESSIKFGDCPICCEELGTNP-LASTK--CGHVYCLKCLERCLK 361
+P+ + CPIC +++ N +TK CGH++ CL++ L+
Sbjct: 95 EPLSNDQLMDLTCPICYDDMNENDEKQATKMPCGHIFGKNCLQKWLE 141
>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1375
Score = 30.3 bits (65), Expect = 0.29
Identities = 17/48 (35%), Positives = 25/48 (52%)
Frame = +2
Query: 218 TSLRKPIIESSIKFGDCPICCEELGTNPLASTKCGHVYCLKCLERCLK 361
T+L + I+ + C IC + + + T CGH+YC CLE LK
Sbjct: 1076 TNLYEHIVLKAESHQICIICRDIIKQGFI--TTCGHLYCSFCLEAWLK 1121
>SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 29.1 bits (62), Expect = 0.68
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +2
Query: 266 CPICCEELGTNPLASTKCGHVYCLKCL 346
C +C E + A+T+CGH++C C+
Sbjct: 256 CSLCMEFIHCP--AATECGHIFCWSCI 280
>SPCC970.10c |brl2|rfp1|ubiquitin-protein ligase E3
Brl2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 680
Score = 28.7 bits (61), Expect = 0.90
Identities = 10/36 (27%), Positives = 22/36 (61%)
Frame = +2
Query: 266 CPICCEELGTNPLASTKCGHVYCLKCLERCLKTEKK 373
C +C E + + S CGH +C +C+++ ++T ++
Sbjct: 627 CSVCNFERWKDRIISL-CGHGFCYQCIQKRIETRQR 661
>SPAC23A1.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 251
Score = 28.7 bits (61), Expect = 0.90
Identities = 15/46 (32%), Positives = 22/46 (47%), Gaps = 7/46 (15%)
Frame = +2
Query: 263 DCPIC--CEELGTNPLA-----STKCGHVYCLKCLERCLKTEKKMS 379
+C +C C + G +A +T CGH YC C+ LK +S
Sbjct: 188 ECGLCMMCVQRGDERVAITTPYTTDCGHTYCYACIMSRLKLVNNVS 233
>SPCC1494.04c |tyr1||prephenate dehydrogenase [NADP+]
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 431
Score = 27.9 bits (59), Expect = 1.6
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = -2
Query: 462 ILDYYNITNFPKIEDDMHNSLSIL 391
+LD Y+I+N PK E ++ LSIL
Sbjct: 292 LLDQYSISNIPKDESKRNSHLSIL 315
>SPAC343.03 |apc11||anaphase-promoting complex subunit
Apc11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 94
Score = 27.9 bits (59), Expect = 1.6
Identities = 11/35 (31%), Positives = 16/35 (45%)
Frame = +2
Query: 260 GDCPICCEELGTNPLASTKCGHVYCLKCLERCLKT 364
G CP C P+ KC H++ C++ L T
Sbjct: 33 GCCPQCTSPGDNCPIVWGKCKHIFHAHCIQNWLAT 67
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 27.9 bits (59), Expect = 1.6
Identities = 21/58 (36%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
Frame = -2
Query: 672 VVTYQSEKLLSIFYLLCRL*LVKPVQPFFETLINSI-TKTMIILVTYKQNTFIFLLNY 502
VV ++KLLS+ L +VK V PF E L++ I K + L + Q I +NY
Sbjct: 1212 VVRSSTQKLLSLLSELSNTPIVKLVSPFKERLLSPIFAKPLRALPFHIQIGHIDAVNY 1269
>SPBC776.18c |pmh1|mcr1|transcription factor TFIIH complex subunit
Pmh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 318
Score = 26.6 bits (56), Expect = 3.6
Identities = 12/37 (32%), Positives = 18/37 (48%), Gaps = 4/37 (10%)
Frame = +2
Query: 266 CPICCEELGTNP----LASTKCGHVYCLKCLERCLKT 364
CP+C + NP L + +C H C C++R T
Sbjct: 13 CPLCQADRYLNPNMKLLINPECYHKMCESCVDRIFTT 49
>SPBC216.06c |swi1||replication fork protection complex subunit
Swi1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 971
Score = 26.2 bits (55), Expect = 4.8
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = +3
Query: 93 KKQSTMSFRDEIIDLTDSFTLANHCALADFIIELDEES 206
+K+ M + IID T NH A +I++ D++S
Sbjct: 884 RKRKKMKTNETIIDHTTRKKKENHLRSAKYIVDSDDDS 921
>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 486
Score = 26.2 bits (55), Expect = 4.8
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +2
Query: 263 DCPICCEELGTNPLASTKCGHVYCLKCLERCL 358
+C IC L +P+ S CGH +C CL + L
Sbjct: 168 ECQICFGML-YDPVVSP-CGHTFCGPCLMQAL 197
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 25.8 bits (54), Expect = 6.3
Identities = 16/64 (25%), Positives = 29/64 (45%)
Frame = -1
Query: 205 DSSSNSIMKSARAQWFANVNESVKSIISSLKDIVLCFFIKYKLRFLKMFFHKIQTSFI*K 26
+++ MK + W+ N++ + +I+SLK I +K K KI +
Sbjct: 225 ETADKERMKKYKQTWYDNMSRKSEPVITSLKKPDEYTKITFKPDLAKFGMDKIDDDMV-S 283
Query: 25 IVKR 14
I+KR
Sbjct: 284 IIKR 287
>SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1040
Score = 25.4 bits (53), Expect = 8.4
Identities = 10/38 (26%), Positives = 18/38 (47%)
Frame = +2
Query: 266 CPICCEELGTNPLASTKCGHVYCLKCLERCLKTEKKMS 379
C +C + + L CGH C +CL + + + M+
Sbjct: 746 CSLCMDVVA-ELLIIVPCGHFLCRECLTHVITSSEDMA 782
>SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 456
Score = 25.4 bits (53), Expect = 8.4
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = +2
Query: 263 DCPICCEELGTNPLASTKCGHVYCLKCL 346
+C IC + P+ C HV+CL CL
Sbjct: 359 ECAIC-SNVAYKPVR-LGCSHVFCLHCL 384
>SPAC343.18 |rfp2||ubiquitin-protein ligase E3
Rfp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 205
Score = 25.4 bits (53), Expect = 8.4
Identities = 12/35 (34%), Positives = 17/35 (48%), Gaps = 3/35 (8%)
Frame = +2
Query: 266 CPICCEELGTNPLAS---TKCGHVYCLKCLERCLK 361
C C EL ++ S KCGH++C C + K
Sbjct: 147 CAKCGNELVSDEKKSIFAAKCGHLFCSTCAKELRK 181
>SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 25.4 bits (53), Expect = 8.4
Identities = 23/84 (27%), Positives = 39/84 (46%), Gaps = 1/84 (1%)
Frame = -2
Query: 639 IFYLLCRL*LVKPVQPFFETLINSITKTMIILVTYKQNTFIFLLNYLHSYCVSTIN*VTI 460
IF +LC V V F+ T IT + ++ T + + ++LH Y VSTI
Sbjct: 320 IFGILCIAGFV--VNEFYTTRTRIITPSAFKTLSL---TSVMVTSFLHYYIVSTITYYIP 374
Query: 459 LDYYNITNFPKIEDDMHN-SLSIL 391
+ + NI + +H SL+++
Sbjct: 375 VYFQNIKGDGPLMSGVHTLSLAVV 398
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,938,411
Number of Sequences: 5004
Number of extensions: 61130
Number of successful extensions: 161
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 150
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 343230174
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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