BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P07_F_E21
(768 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5 |Schiz... 72 1e-13
SPAC20G4.04c |hus1||checkpoint clamp complex protein Hus1|Schizo... 29 0.97
SPAC926.08c |||Brix doamin protein Rpf2 |Schizosaccharomyces pom... 26 6.8
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 26 6.8
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 25 9.0
SPBC16E9.13 |ksp1|ppk20|serine/threonine protein kinase Ksp1 |Sc... 25 9.0
SPAC22E12.11c |set3||histone lysine methyltransferase Set3|Schiz... 25 9.0
>SPBC4B4.10c |mug77|atg5|autophagy associated protein Atg5
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 261
Score = 71.7 bits (168), Expect = 1e-13
Identities = 35/106 (33%), Positives = 56/106 (52%), Gaps = 5/106 (4%)
Frame = +2
Query: 248 VPRLSYFPLVIDKMKRHFLRFISQENSDSEMWLDFNGQPLKWHYPIGFLYDLYCGNDPQL 427
VPR SYF ++ ++R ++ SE WLD+NG PLKWH+P+G L+DL DP
Sbjct: 36 VPRQSYFAQILPNVQR----LLAPSIPLSECWLDYNGVPLKWHWPVGLLFDLLTVFDPDT 91
Query: 428 P-----WTLTVHFTKFPEDILLHCPNKDVVEAHYMSTVKEADVLKH 550
P W + + FP +L D ++ + +KE+D +++
Sbjct: 92 PRAPVLWRIQLRSGLFPTTKILQMETMDTFRTYFFNCLKESDYVRN 137
>SPAC20G4.04c |hus1||checkpoint clamp complex protein
Hus1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 287
Score = 28.7 bits (61), Expect = 0.97
Identities = 16/50 (32%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = +2
Query: 209 IMEIQQPDP-FYVMVPRLSYFPLVIDKMKRHFLRFISQENSDSEMWLDFN 355
I E P+P ++ +P+L++ V+DK K R I N E+ L N
Sbjct: 140 IKEPTAPEPDCHIFLPQLNFLRHVVDKYKSLSDRIIMSANMSGELQLSVN 189
>SPAC926.08c |||Brix doamin protein Rpf2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 317
Score = 25.8 bits (54), Expect = 6.8
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = -3
Query: 259 KSRHHNVKWIRLLNFHYFFLSKMKTYR*FSIPYFSKYLPVV 137
K R HN+ W+R N+ + ++ SI FS P+V
Sbjct: 94 KKRPHNLTWVRFFNYRVLDMIELGIVNYKSIQSFSA-TPIV 133
>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase
Win1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1436
Score = 25.8 bits (54), Expect = 6.8
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = -3
Query: 538 ISLFHCRHIMCFYYIFVWAMKKNVFRELCE 449
+ LF +++ +Y I V K N+F ELC+
Sbjct: 1173 LELFDHPNVVSYYGIEVHREKVNIFMELCQ 1202
>SPBC119.07 |ppk19||serine/threonine protein kinase
Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1706
Score = 25.4 bits (53), Expect = 9.0
Identities = 13/36 (36%), Positives = 23/36 (63%)
Frame = +1
Query: 622 SLISFGLSTDVLWSHMVIMMDSNTYHLGFILMMVYT 729
+LIS G ++ +S V M+++T+H G I ++V T
Sbjct: 1310 TLISAGRIPNLDFSDSVTSMEASTFHDGSIRLVVVT 1345
>SPBC16E9.13 |ksp1|ppk20|serine/threonine protein kinase Ksp1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 474
Score = 25.4 bits (53), Expect = 9.0
Identities = 16/42 (38%), Positives = 22/42 (52%)
Frame = -2
Query: 542 VHQPLSL*TYNVLLLHLCLGNEEECLQGTL*SVLSKSRVVED 417
V LS TYNVL LC+ E+ L T +VL+ ++ D
Sbjct: 241 VFPTLSQDTYNVLRACLCVSPEKRSLAKTREAVLAVTKWTTD 282
>SPAC22E12.11c |set3||histone lysine methyltransferase
Set3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 859
Score = 25.4 bits (53), Expect = 9.0
Identities = 11/32 (34%), Positives = 21/32 (65%)
Frame = +1
Query: 505 STLYVYSERG*CTETQRTSDVNYAKKRITISY 600
+TL ++ +TQR+SDVN++++ T S+
Sbjct: 684 NTLRTVKDKSHVHDTQRSSDVNFSRQNGTRSH 715
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,278,061
Number of Sequences: 5004
Number of extensions: 71866
Number of successful extensions: 164
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 369323696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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