Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P07_F_D17
(756 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 66 5e-12
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 65 9e-12
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 52 9e-08
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 29 0.54
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.7
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 6.7
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 66.1 bits (154), Expect = 5e-12
Identities = 42/136 (30%), Positives = 69/136 (50%)
Frame = +3
Query: 117 AFSIYXFEGKGKXDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQA 296
AFS++ G G+ ++GDLLRA NPTLA I FL + ++
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVDMEQ---FLQVLNRP 67
Query: 297 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 476
G E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K
Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124
Query: 477 DDGMIPYAAFLKKVMA 524
DGM+ Y F++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 65.3 bits (152), Expect = 9e-12
Identities = 41/135 (30%), Positives = 67/135 (49%), Gaps = 1/135 (0%)
Frame = +3
Query: 117 AFSIYXFEGKGKXDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXX-FLPIYSQ 293
AFS++ + G + LG ++R+L +PT A + FL + ++
Sbjct: 17 AFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMAR 76
Query: 294 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 473
KD D E+ E K++DK+ NG + ELTH L +LGE+L EVA++ ++
Sbjct: 77 KMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA--DT 132
Query: 474 DDDGMIPYAAFLKKV 518
D DG+I Y F + +
Sbjct: 133 DGDGVINYEEFSRVI 147
Score = 29.1 bits (62), Expect = 0.72
Identities = 15/61 (24%), Positives = 32/61 (52%)
Frame = +3
Query: 327 DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 506
+F E L+D++++G + EL + +LG+ +E+ ++ + D +G I + F
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEF 70
Query: 507 L 509
L
Sbjct: 71 L 71
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 52.0 bits (119), Expect = 9e-08
Identities = 34/148 (22%), Positives = 70/148 (47%)
Frame = +3
Query: 81 PSASHXVERASFAFSIYXFEGKGKXDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXX 260
P++ + AF +Y + G ++G +LR+L N T A +
Sbjct: 2 PASKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEK 61
Query: 261 XXXXFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEV 440
F+ + K ++ + E++++ +++DK+ +G + A+ + LGEKL D+EV
Sbjct: 62 KFMSFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEV 116
Query: 441 AEVTKDCMDPEDDDGMIPYAAFLKKVMA 524
+ ++ DP + G Y F++++MA
Sbjct: 117 QLMVQEA-DP-TNSGSFDYYDFVQRIMA 142
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 29.5 bits (63), Expect = 0.54
Identities = 15/58 (25%), Positives = 30/58 (51%)
Frame = +3
Query: 285 YSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKD 458
Y+ + + + +D E KL+D +++ + EL + ALG + SEV ++ +D
Sbjct: 24 YAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81
Score = 27.1 bits (57), Expect = 2.9
Identities = 18/75 (24%), Positives = 33/75 (44%)
Frame = +3
Query: 297 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 476
+K ++ E+ +L+D +E G + L L E +DD E+ + ++ D
Sbjct: 101 EKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DLD 158
Query: 477 DDGMIPYAAFLKKVM 521
DG I F+ +M
Sbjct: 159 QDGEINEQEFIAIMM 173
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 1.7
Identities = 13/31 (41%), Positives = 15/31 (48%)
Frame = -3
Query: 673 APPEELSPPRALPAPVPQSRASVF*GPSHRT 581
APP PP A P P+P S A P R+
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 6.7
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +3
Query: 411 LGEKLDDSEVAEVTKDCMDPED 476
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,553,302
Number of Sequences: 5004
Number of extensions: 42818
Number of successful extensions: 113
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 102
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 110
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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