Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P07_F_D09
(874 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 397 e-111
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 394 e-110
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 204 2e-53
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 165 7e-42
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 32 0.093
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.1
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.1
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 397 bits (977), Expect = e-111
Identities = 177/252 (70%), Positives = 211/252 (83%)
Frame = +3
Query: 114 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 293
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 294 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 473
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 474 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 653
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 654 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGXIVSSITA 833
VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI +VSSITA
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITA 240
Query: 834 SLXFDGALNVDL 869
SL F+G+LNVDL
Sbjct: 241 SLRFEGSLNVDL 252
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 394 bits (969), Expect = e-110
Identities = 181/257 (70%), Positives = 213/257 (82%), Gaps = 5/257 (1%)
Frame = +3
Query: 114 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 278
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 279 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 458
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 459 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAP 638
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 639 QVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGXIV 818
QVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI +V
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVV 239
Query: 819 SSITASLXFDGALNVDL 869
SSITASL F G+LNVDL
Sbjct: 240 SSITASLRFAGSLNVDL 256
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 204 bits (497), Expect = 2e-53
Identities = 99/252 (39%), Positives = 143/252 (56%)
Frame = +3
Query: 114 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 293
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 294 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 473
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 474 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQVSTA 653
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 654 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGXIVSSITA 833
VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L+ +++ +T
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTT 238
Query: 834 SLXFDGALNVDL 869
S F G LN DL
Sbjct: 239 SFRFPGELNSDL 250
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 165 bits (401), Expect = 7e-42
Identities = 89/254 (35%), Positives = 142/254 (55%), Gaps = 3/254 (1%)
Frame = +3
Query: 117 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 296
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 297 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVL 470
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 471 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFAIYPAPQ-VS 647
D I + AD L+GF + H L+ERL+ Y KK ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 648 TAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGXIVSSI 827
VV+PYNS+L ++D ++DN A+ I L + PT+ N+L+ ++S+
Sbjct: 180 DVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSAS 239
Query: 828 TASLXFDGALNVDL 869
T +L + G +N DL
Sbjct: 240 TTTLRYPGYMNNDL 253
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 32.3 bits (70), Expect = 0.093
Identities = 16/43 (37%), Positives = 26/43 (60%)
Frame = -3
Query: 863 HIQSAVEXQRSSDRGDNXSDETIQVGVGWALNVEITAADVIDG 735
H ++ + Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 176 HRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.1
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +1
Query: 193 STASSLMARCPQTRPSGVETILSTLSSARPELAST 297
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 212 WPDAHRQDHRGWRRFFQHFLQR 277
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,441,805
Number of Sequences: 5004
Number of extensions: 70343
Number of successful extensions: 216
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 205
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 211
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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