Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P07_F_C19
(769 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC27F1.03c |uch1||ubiquitin C-terminal hydrolase Uch1|Schizosa... 98 1e-21
SPBC409.06 |uch2||ubiquitin C-terminal hydrolase Uch2|Schizosacc... 92 7e-20
SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces ... 29 0.97
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar... 27 2.2
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 27 2.2
SPAC24C9.11 |||MIF4G/MA4 domain protein|Schizosaccharomyces pomb... 27 2.2
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.9
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 27 3.9
SPBC106.08c |mug2||DUF1773 family protein 1|Schizosaccharomyces ... 25 9.0
SPAPB1A10.12c |alo1||D-arabinono-1,4-lactone oxidase|Schizosacch... 25 9.0
>SPAC27F1.03c |uch1||ubiquitin C-terminal hydrolase
Uch1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 222
Score = 98.3 bits (234), Expect = 1e-21
Identities = 67/211 (31%), Positives = 108/211 (51%), Gaps = 6/211 (2%)
Frame = +2
Query: 110 PLESNPDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHKK 289
PLE+ P+VL +LQK+GV + ++ D+ L+ E ++PRPV +++ +FP S +K
Sbjct: 4 PLENTPEVLEPYLQKIGVQDA-SVFDLFSLE-EIPEYIPRPVHALLFVFPSSGTKTIYKG 61
Query: 290 TEENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDIIELSDG-HMQKFLNEAK 466
+ IL K S + + Q I NACGTI L+H+V+N ++++ ++ + A+
Sbjct: 62 SR---ILPKD---SDKVLWYPQTIPNACGTIGLLHAVSNGELRRKVNENDFIKSLIRTAE 115
Query: 467 GLDATARGKLLEKSEGIINAHKELAQEGQTNTPSAED-PVNHHFISFV----QKDGALYE 631
G R KL+E S+ + H A S ED + HFI FV + D YE
Sbjct: 116 GSSIEERAKLIEDSKELEALHAAFAGPPLEVEGSEEDVETDLHFICFVKGKSKDDNHFYE 175
Query: 632 LDGRKAFPVNHGPTSQETLLEDAAKICKEFM 724
LDGR+ PV H + L + + K ++
Sbjct: 176 LDGRQEGPVQHSEIESDLLNAEVLSVIKNYI 206
>SPBC409.06 |uch2||ubiquitin C-terminal hydrolase
Uch2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 300
Score = 92.3 bits (219), Expect = 7e-20
Identities = 64/224 (28%), Positives = 113/224 (50%), Gaps = 6/224 (2%)
Frame = +2
Query: 113 LESNPDVLNKFLQKLGVPNKWNIVDVMGLDPETLSWVPRPVLSVMLLFPISDAYENHKKT 292
+ES+ V ++ LGV + + ++ LD ++L P + ++ LF + + T
Sbjct: 6 IESDAGVFTDLIENLGVKDV-EVDELYSLDVDSLRQFP-DIYGIIFLFKWNSKVDKPDGT 63
Query: 293 EENEILSKGQEVSGNIFYMKQNISNACGTIALVHSVANNTDIIELSDGHMQKFLNEAKGL 472
+ + + NIF+ KQ I+NAC T AL+ + N++D I+L + +F + +K L
Sbjct: 64 MDYDSMD-------NIFFAKQVINNACATQALLSVLLNHSDEIDLGTT-LSEFKDFSKTL 115
Query: 473 DATARGKLLEKSEGIINAHKELAQEG-----QTNTPSAEDPVNHHFISFVQKDGALYELD 637
+G+ L SE I H A+ + + ED V +HFI++ + YELD
Sbjct: 116 PPELKGEALGNSEHIRCCHNSFARSDPFISEEVRAATDEDEV-YHFIAYTNINNVFYELD 174
Query: 638 GRKAFPVNHGPTSQETLLEDAAKICKEFMAR-DPNEVRFTVIAL 766
G +A P+NHG ++E E A + + +A DP E+RF ++ +
Sbjct: 175 GLQAAPINHGSCTKEEFAEKAVSVIQARIANYDPAEIRFNLMVI 218
>SPCC74.01 |sly1||SNARE binding protein Sly1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 639
Score = 28.7 bits (61), Expect = 0.97
Identities = 17/62 (27%), Positives = 28/62 (45%)
Frame = +2
Query: 506 SEGIINAHKELAQEGQTNTPSAEDPVNHHFISFVQKDGALYELDGRKAFPVNHGPTSQET 685
S ++ ELA + TNT V ++++V + + L K F H P+S E
Sbjct: 126 SRALLEQFAELASK--TNTSHMIHQVYDQYLNYVVLESDFFSLQLPKIFHTFHNPSSDEA 183
Query: 686 LL 691
L+
Sbjct: 184 LI 185
>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 27.5 bits (58), Expect = 2.2
Identities = 14/36 (38%), Positives = 17/36 (47%)
Frame = +3
Query: 612 KMEHCMS*MAVKLFLSIMAQLHRKLCWKMQQKFAKN 719
+M HC S L I L R L W+ +QK A N
Sbjct: 508 RMSHCSSNSNKVLASEISESLRRDLLWERRQKAAMN 543
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2244
Score = 27.5 bits (58), Expect = 2.2
Identities = 19/80 (23%), Positives = 38/80 (47%), Gaps = 1/80 (1%)
Frame = +2
Query: 515 IINAHKELAQEGQTNTPSAEDPVNHHFISFVQKDGALYELDGRKAFPVNH-GPTSQETLL 691
+I+ + E A+E + + + E + H IS ++ ++ D P PT+ E ++
Sbjct: 1205 VISKYIENAKEIELDAVAREGKMVMHVISEHVENAGVHSGDATLVLPPQDLAPTTIERIV 1264
Query: 692 EDAAKICKEFMARDPNEVRF 751
+ AAKI + P ++F
Sbjct: 1265 DAAAKIGEALNITGPYNIQF 1284
>SPAC24C9.11 |||MIF4G/MA4 domain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 775
Score = 27.5 bits (58), Expect = 2.2
Identities = 16/48 (33%), Positives = 22/48 (45%)
Frame = -1
Query: 196 SHDIYNIPFVWYAELLQKLVQDIGIGFKRD*SFGRHFXHSNCGVDEGD 53
+H + F W E L + + DIG+ D S+G H S D GD
Sbjct: 133 AHSKIDKEFGWLLEDLDQEIDDIGVPGTEDPSYG-HSEDSEVSDDTGD 179
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.9
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +2
Query: 569 AEDPVNHHFISFVQKDGALYELDGR 643
AE+ V HF+ KD LY++D R
Sbjct: 1099 AEESVKRHFLRLWLKDNGLYDVDIR 1123
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 26.6 bits (56), Expect = 3.9
Identities = 10/31 (32%), Positives = 22/31 (70%)
Frame = +2
Query: 518 INAHKELAQEGQTNTPSAEDPVNHHFISFVQ 610
++A + L ++ +++T + E+PV+HH S V+
Sbjct: 337 LSARRALFEKKESSTKNVENPVSHHLKSPVR 367
>SPBC106.08c |mug2||DUF1773 family protein 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 296
Score = 25.4 bits (53), Expect = 9.0
Identities = 21/77 (27%), Positives = 38/77 (49%), Gaps = 1/77 (1%)
Frame = +2
Query: 404 NNTDIIELSDGHMQ-KFLNEAKGLDATARGKLLEKSEGIINAHKELAQEGQTNTPSAEDP 580
+NT+++ + ++ K ++ K +D + G L + E +H + GQ +DP
Sbjct: 31 SNTNMLSTEENIVKAKAVSRKKPVDVSYAG--LARHE----SHVDTDYHGQALNTDNQDP 84
Query: 581 VNHHFISFVQKDGALYE 631
N+ SFV K+ LYE
Sbjct: 85 KNYQARSFV-KENELYE 100
>SPAPB1A10.12c |alo1||D-arabinono-1,4-lactone
oxidase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 461
Score = 25.4 bits (53), Expect = 9.0
Identities = 17/47 (36%), Positives = 21/47 (44%), Gaps = 8/47 (17%)
Frame = +2
Query: 107 VPLESNPDVLNKFLQ-------KLGVPNKWNI-VDVMGLDPETLSWV 223
+PLES PD L K + K+G W I V V PE W+
Sbjct: 326 IPLESAPDALEKLINYTVDDAGKIGAYTHWPIEVRVCAPTPEDECWL 372
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,240,799
Number of Sequences: 5004
Number of extensions: 68624
Number of successful extensions: 203
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 199
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 369323696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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