Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P07_F_B22
(732 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 29 0.52
SPBC25H2.06c |hrf1||COPII-coated vesicle component Hrf1 |Schizos... 28 1.6
SPAC4F8.08 |mug114||sequence orphan|Schizosaccharomyces pombe|ch... 28 1.6
SPBC17D11.07c |rpn2||19S proteasome regulatory subunit Rpn2|Schi... 26 4.8
SPAC8E11.01c ||SPAC959.01|beta-fructofuranosidase|Schizosaccharo... 26 4.8
SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces pomb... 26 4.8
SPAC9E9.15 |||CIA30 family protein|Schizosaccharomyces pombe|chr... 26 6.4
SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces ... 25 8.4
SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 25 8.4
SPBP8B7.04 |mug45||sequence orphan|Schizosaccharomyces pombe|chr... 25 8.4
>SPAC19A8.08 |upf2||nonsense-mediated decay protein
Upf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1049
Score = 29.5 bits (63), Expect = 0.52
Identities = 13/66 (19%), Positives = 35/66 (53%)
Frame = +1
Query: 517 MRDTNGNTREASVTKSFANFIDVNGTVVQNIVSNEITKLYHSLSSEKKEK*LILSICNLS 696
++D+ G+ V+ NF+ ++V ++NE+ ++ L+++ LI ++C +
Sbjct: 429 LKDSTGDLNTTQVSSRVDNFLLKLPSMVSLELTNEMALEFYDLNTKASRNRLIKALCTIP 488
Query: 697 RSDIYV 714
R+ ++
Sbjct: 489 RTSSFL 494
>SPBC25H2.06c |hrf1||COPII-coated vesicle component Hrf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 293
Score = 27.9 bits (59), Expect = 1.6
Identities = 19/72 (26%), Positives = 35/72 (48%), Gaps = 2/72 (2%)
Frame = -2
Query: 635 YSLVISLDTMF*TTVPFTSMKLANDLVTDASRV--LPFVSRITITRLYLSSCFFT*ELAS 462
Y L IS + + F+ K ++T S++ +P+V+R +YL++ FF L S
Sbjct: 192 YLLNISSQSQVLDLLAFSGYKFVGLILTSLSKLFEMPWVTRFVFLYMYLATAFFL--LRS 249
Query: 461 QTLVLFPTFSLA 426
+ P ++A
Sbjct: 250 LKYAVLPESTMA 261
>SPAC4F8.08 |mug114||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 151
Score = 27.9 bits (59), Expect = 1.6
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +1
Query: 403 KEKGIFVVAKEKVGNNTRVWEASSYVK 483
K+ G+F +AK K+ N T+VW + Y K
Sbjct: 4 KKGGLFKIAK-KLRNGTKVWARAGYFK 29
>SPBC17D11.07c |rpn2||19S proteasome regulatory subunit
Rpn2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 965
Score = 26.2 bits (55), Expect = 4.8
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = +3
Query: 507 RNCYARHEWQHSRGI 551
+ CYA++EW+H GI
Sbjct: 147 QKCYAKNEWRHVLGI 161
>SPAC8E11.01c
||SPAC959.01|beta-fructofuranosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 508
Score = 26.2 bits (55), Expect = 4.8
Identities = 10/24 (41%), Positives = 15/24 (62%), Gaps = 1/24 (4%)
Frame = +2
Query: 269 WLLVWPFTHCYGIIFIHSLN-QDW 337
W++V Y ++F HSLN +DW
Sbjct: 162 WIMVVVLAQKYKVLFYHSLNLRDW 185
>SPBC216.05 |rad3||ATR checkpoint kinase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 2386
Score = 26.2 bits (55), Expect = 4.8
Identities = 11/41 (26%), Positives = 23/41 (56%)
Frame = +1
Query: 574 FIDVNGTVVQNIVSNEITKLYHSLSSEKKEK*LILSICNLS 696
F+ ++ +VQ + + +YH L +K K +++S NL+
Sbjct: 275 FLHLHSRIVQTLCCFSLNFIYHGLPICEKSKHILMSSINLT 315
>SPAC9E9.15 |||CIA30 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 219
Score = 25.8 bits (54), Expect = 6.4
Identities = 15/47 (31%), Positives = 25/47 (53%)
Frame = -2
Query: 701 DLLKLHIERINYFSFFSDERLWYSLVISLDTMF*TTVPFTSMKLAND 561
D+ K+ I SFF+ + Y LV++ VPFT+ K++N+
Sbjct: 152 DVSKITQFSIMIRSFFNSQSGDYELVLNSIRAIPKNVPFTTHKMSNE 198
>SPAC3H1.11 |hsr1||transcription factor Hsr1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 582
Score = 25.4 bits (53), Expect = 8.4
Identities = 12/33 (36%), Positives = 15/33 (45%)
Frame = -3
Query: 508 RDCIYHRVSSHKNWLPRPWCYFQPSPWLPQRCP 410
RD + H N PR C+FQPS + P
Sbjct: 463 RDNLRQHERLHVNASPRLACFFQPSGYYSSGAP 495
>SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1242
Score = 25.4 bits (53), Expect = 8.4
Identities = 17/64 (26%), Positives = 26/64 (40%)
Frame = +1
Query: 385 TLYTTFKEKGIFVVAKEKVGNNTRVWEASSYVKKHDDKYNLVIVMRDTNGNTREASVTKS 564
T++ K +GIF + ++V NN W D Y +I T V +S
Sbjct: 527 TVFVKLKLQGIFSSSFQQVSNNMYSWIYDHVFSSSDHAYESLIYYMKTG----HKQVVQS 582
Query: 565 FANF 576
F+ F
Sbjct: 583 FSIF 586
>SPBP8B7.04 |mug45||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 819
Score = 25.4 bits (53), Expect = 8.4
Identities = 19/59 (32%), Positives = 26/59 (44%)
Frame = +1
Query: 490 DDKYNLVIVMRDTNGNTREASVTKSFANFIDVNGTVVQNIVSNEITKLYHSLSSEKKEK 666
D K+ LV GN R F + VN +QN VSN++ K +S + E K
Sbjct: 41 DRKWELVCCKELNIGNERNPLNVPLFE--LPVNSLELQNSVSNDMFKHVYSQNVELNHK 97
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,829,260
Number of Sequences: 5004
Number of extensions: 57334
Number of successful extensions: 147
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 345237368
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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