Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P07_F_B21
(724 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1105.18c ||SPBC887.21c|peptide release factor|Schizosaccharo... 76 6e-15
SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces p... 50 4e-07
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 36 0.006
SPBC13G1.06c |isd11||iron sulfur cluster assembly protein Isd11|... 31 0.17
SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|ch... 28 1.2
SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyc... 28 1.2
SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces p... 28 1.6
SPAC589.11 |mug82||translation release factor |Schizosaccharomyc... 28 1.6
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 27 3.6
SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces... 26 4.7
SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogam... 26 4.7
SPBC13G1.11 |ykt6||SNARE Ykt6|Schizosaccharomyces pombe|chr 2|||... 26 6.3
SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyce... 25 8.3
SPMIT.06 |||mitochondrial DNA binding endonuclease|Schizosacchar... 25 8.3
SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces pomb... 25 8.3
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 25 8.3
SPBC887.08 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 8.3
SPAC22G7.05 |||krr family protein|Schizosaccharomyces pombe|chr ... 25 8.3
>SPBC1105.18c ||SPBC887.21c|peptide release
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 162
Score = 75.8 bits (178), Expect = 6e-15
Identities = 33/106 (31%), Positives = 68/106 (64%), Gaps = 2/106 (1%)
Frame = +2
Query: 362 KVPKINECELSEKFVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKCHTSRCQDDNRKKARE 541
++ ++ E ++ E F+ G GPGG +NK S + HIPTG +++ +R ++ NR AR+
Sbjct: 43 QLERLQEEDIEETFICGKGPGGQKINKTSIVAQVKHIPTGIIVRSQDTRSREQNRCIARK 102
Query: 542 LLIEKLDDMINGPESVSAQR--KLIEEKKYKRNATKKQKISKLKDE 673
L EK+D+ +G +S+ A++ +++++KK + +K++ +K+ D+
Sbjct: 103 RLTEKVDEFKHGNDSLLARKVQRIVKKKKQREKKSKRKYGNKIDDQ 148
>SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 396
Score = 49.6 bits (113), Expect = 4e-07
Identities = 28/90 (31%), Positives = 48/90 (53%)
Frame = +2
Query: 386 ELSEKFVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKCHTSRCQDDNRKKARELLIEKLDD 565
E+ + +R G GG VN+ + V LTHIPTG + SR Q N++KA +L +L
Sbjct: 256 EVKIEVMRSRGAGGQHVNRTESAVRLTHIPTGITVSMQDSRSQHQNKEKAFLVLNSRL-A 314
Query: 566 MINGPESVSAQRKLIEEKKYKRNATKKQKI 655
+N + A+R ++ K ++ + +K+
Sbjct: 315 ALNAAKENEAER--LKRKNQVTSSDRSEKL 342
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 35.9 bits (79), Expect = 0.006
Identities = 19/54 (35%), Positives = 33/54 (61%), Gaps = 1/54 (1%)
Frame = +2
Query: 527 KKARELLIEK-LDDMINGPESVSAQRKLIEEKKYKRNATKKQKISKLKDEWKKR 685
K+ E++++ +D+ + ES+SA RK + K Y + T+KQK+ + E KKR
Sbjct: 575 KQLYEVILQAVIDNRVVARESISALRKNVTAKCYGGDVTRKQKLLNKQKEGKKR 628
>SPBC13G1.06c |isd11||iron sulfur cluster assembly protein
Isd11|Schizosaccharomyces pombe|chr 2|||Manual
Length = 102
Score = 31.1 bits (67), Expect = 0.17
Identities = 21/70 (30%), Positives = 36/70 (51%), Gaps = 3/70 (4%)
Frame = +1
Query: 76 MXLTRKAVLRLYXNLLMYSKSLKLTDPDYFRRSIKIEFKNNQNLTDLEKISFAYQKGQAL 255
M ++++ V+RLY N+L SK T +Y R + +FK + +D K + + L
Sbjct: 1 MSVSKQHVVRLYRNILKTSKLFPYTYREYTIRRTRDKFKELKVESDPAKFEQGIKDSEKL 60
Query: 256 L---QRGSVV 276
L QR S++
Sbjct: 61 LEIIQRQSII 70
>SPAC27D7.02c |||GRIP domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 750
Score = 28.3 bits (60), Expect = 1.2
Identities = 21/64 (32%), Positives = 34/64 (53%)
Frame = +2
Query: 518 DNRKKARELLIEKLDDMINGPESVSAQRKLIEEKKYKRNATKKQKISKLKDEWKKREGII 697
+N K E IEKL+ I+ S+ Q+K EK +R + ++KI+ L+ + +I
Sbjct: 122 ENLKHQFEDQIEKLNSEISNQNSLILQKKDELEKSIQRCSELEEKINSLESAQSIEQEVI 181
Query: 698 *SSL 709
SSL
Sbjct: 182 -SSL 184
>SPCC4B3.15 |mid1|dmf1|medial ring protein Mid1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 920
Score = 28.3 bits (60), Expect = 1.2
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = +2
Query: 515 DDNRKKARELLIEKLDDMINGPESVSAQRKLIEEKKYKRNATKKQKISKLKDEWKKRE 688
DDN K + L ++D NG + VSA + + K + + ++ KL + KKR+
Sbjct: 638 DDNFKVSFMFLDIPIEDKSNGSKGVSATKDVSNGKPAETKSKARKFFDKLFNRRKKRK 695
>SPBC31E1.05 |gle1||RNA export factor Gle1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 480
Score = 27.9 bits (59), Expect = 1.6
Identities = 29/123 (23%), Positives = 54/123 (43%), Gaps = 1/123 (0%)
Frame = +2
Query: 323 RLAAAFKHTIDYSKVPKINECELSEKFVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKCHT 502
R+ TID++K + E L E+ + S S + + I+
Sbjct: 68 RIFPPLHKTIDWAKKRNVEEQNLIEQSITESQRIFSEKQRLEQERFNRELLEKKRIEAER 127
Query: 503 SRCQDDNRKKARELLIEKLDDMINGPESVSAQR-KLIEEKKYKRNATKKQKISKLKDEWK 679
R +D+ ++ +EL+ EK E +R +LIEE+K+K N ++ K ++ + K
Sbjct: 128 QRLKDEEERRKKELM-EK--------EKKEKERIRLIEEQKHKENEQRRLKQEQIDAKRK 178
Query: 680 KRE 688
+ E
Sbjct: 179 EEE 181
>SPAC589.11 |mug82||translation release factor |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 182
Score = 27.9 bits (59), Expect = 1.6
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = +2
Query: 401 FVRGSGPGGSAVNKNSNCVVLTHIPTGTVIKC 496
F R SGPGG VNK N V+ ++P + C
Sbjct: 53 FSRSSGPGGQNVNK-LNTKVIVNLPFKQLESC 83
>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 26.6 bits (56), Expect = 3.6
Identities = 14/60 (23%), Positives = 32/60 (53%), Gaps = 1/60 (1%)
Frame = +2
Query: 512 QDDNRKKARELLIEKLDDMINGPESVSAQR-KLIEEKKYKRNATKKQKISKLKDEWKKRE 688
++ ++K RE ++K + + QR + EEK+ +++K+ K ++E ++RE
Sbjct: 663 EEKQKQKEREKKLKKQQQEADREKMAREQRLREEEEKRILEERKRREKLDKEEEERRRRE 722
Score = 25.8 bits (54), Expect = 6.3
Identities = 12/38 (31%), Positives = 26/38 (68%), Gaps = 2/38 (5%)
Frame = +2
Query: 581 ESVSAQR--KLIEEKKYKRNATKKQKISKLKDEWKKRE 688
E V+ QR KL+EE + + +++++ K++++ KKR+
Sbjct: 562 EKVAQQRQAKLLEEIEEENKRKQERELKKIREKEKKRD 599
>SPAC1952.03 |||cysteine protease, OTU family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 324
Score = 26.2 bits (55), Expect = 4.7
Identities = 14/58 (24%), Positives = 30/58 (51%)
Frame = +2
Query: 512 QDDNRKKARELLIEKLDDMINGPESVSAQRKLIEEKKYKRNATKKQKISKLKDEWKKR 685
Q KK ELL ++ ++ ++ RK ++E K+ +QKIS+++ + ++
Sbjct: 18 QKRKSKKMEELLSKQREECKELQSKITNLRKQLKEGNKKQKRALQQKISQMEADLSQK 75
>SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogamy
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 543
Score = 26.2 bits (55), Expect = 4.7
Identities = 14/53 (26%), Positives = 23/53 (43%)
Frame = +2
Query: 116 IC*CTRNH*NSPTQIIFGAQLKSNSKITKT*LIWRKLASRIRKVRPCCREGQL 274
+C R+ +P++ + G+Q + SK+ T W S V CR L
Sbjct: 64 LCDFERSQILAPSECVRGSQSECVSKLESTSTWWLSFTSHFHDVNHLCRLANL 116
>SPBC13G1.11 |ykt6||SNARE Ykt6|Schizosaccharomyces pombe|chr
2|||Manual
Length = 197
Score = 25.8 bits (54), Expect = 6.3
Identities = 10/29 (34%), Positives = 19/29 (65%)
Frame = +2
Query: 551 EKLDDMINGPESVSAQRKLIEEKKYKRNA 637
EKLDD+I +++S Q ++ + K+N+
Sbjct: 164 EKLDDLIQRSDNLSTQSRMFYKSAKKQNS 192
>SPAC20G8.06 |||CCR4-Not complex subunit Not1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2100
Score = 25.4 bits (53), Expect = 8.3
Identities = 15/38 (39%), Positives = 24/38 (63%)
Frame = +1
Query: 109 YXNLLMYSKSLKLTDPDYFRRSIKIEFKNNQNLTDLEK 222
Y NL++ SL+L PD + SI+ +N+N T++EK
Sbjct: 789 YCNLILGIPSLQLVRPDIY-DSIREMIASNEN-TEVEK 824
>SPMIT.06 |||mitochondrial DNA binding
endonuclease|Schizosaccharomyces pombe|chr
mitochondrial|||Manual
Length = 807
Score = 25.4 bits (53), Expect = 8.3
Identities = 9/19 (47%), Positives = 14/19 (73%)
Frame = -3
Query: 713 HKEKIILCLPVSSIHLLIW 657
H+E+ ILC+ + +IHL W
Sbjct: 11 HRERDILCVKIWTIHLGSW 29
>SPAC2C4.17c |||MS ion channel protein 2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 840
Score = 25.4 bits (53), Expect = 8.3
Identities = 11/32 (34%), Positives = 22/32 (68%), Gaps = 1/32 (3%)
Frame = +1
Query: 127 YSKSLKLTDPDYFRRSIKIEFKN-NQNLTDLE 219
YS+++ L + + F R I IEF++ + +L D++
Sbjct: 408 YSRTVTLDEMEQFTREISIEFRSISSSLRDVD 439
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 25.4 bits (53), Expect = 8.3
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +2
Query: 341 KHTIDYSKVPKINECELSEKFVRGSGPG 424
++TI SK+PK+ E E +K + G G
Sbjct: 221 QNTIYESKIPKLIEHETIQKIAKSKGEG 248
>SPBC887.08 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 124
Score = 25.4 bits (53), Expect = 8.3
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = +2
Query: 608 IEEKKYKRNATKKQKISKLKDEWKKR 685
+ E K +RN KQK+S D W R
Sbjct: 12 VSESKLERNKRLKQKVSIETDGWTPR 37
>SPAC22G7.05 |||krr family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 598
Score = 25.4 bits (53), Expect = 8.3
Identities = 17/53 (32%), Positives = 29/53 (54%)
Frame = +2
Query: 506 RCQDDNRKKARELLIEKLDDMINGPESVSAQRKLIEEKKYKRNATKKQKISKL 664
R +DD+RK+AR+ E+L++ A +K +EE +N +K+ KL
Sbjct: 366 RRKDDSRKRARDRKKERLEE---------ASQKRLEEVNRLKNLKRKELEEKL 409
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,880,840
Number of Sequences: 5004
Number of extensions: 58812
Number of successful extensions: 202
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 202
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 339215786
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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