Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P06_pT_N20
(770 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 70 4e-13
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 70 4e-13
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 56 6e-09
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 31 0.24
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 5.2
SPBC13G1.03c |pex14||peroxisomal membrane anchor protein|Schizos... 26 6.9
SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pomb... 26 6.9
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 6.9
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 26 6.9
SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces pombe... 25 9.1
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 69.7 bits (163), Expect = 4e-13
Identities = 47/151 (31%), Positives = 75/151 (49%), Gaps = 3/151 (1%)
Frame = -1
Query: 734 MSDLSKNDVERASF--AFSXYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXX 561
M+ + D + A F AFS +D + G I + LG ++R+L +PT A +
Sbjct: 1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDAD 60
Query: 560 XXXXXXXE-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKL 384
FL + ++ KD D E+ E K++DK+ NG + ELTH L +LGE+L
Sbjct: 61 GNGTIDFTEFLTMMARKMKDTDNE--EEVREAFKVFDKDGNGYITVEELTHVLTSLGERL 118
Query: 383 DDSEVAEVTKDCMDPEDDDGMIPYAAFLKKV 291
EVA++ ++ D DG+I Y F + +
Sbjct: 119 SQEEVADMIREA--DTDGDGVINYEEFSRVI 147
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 69.7 bits (163), Expect = 4e-13
Identities = 44/136 (32%), Positives = 71/136 (52%)
Frame = -1
Query: 692 AFSXYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXXXEFLPIYSQA 513
AFS +D G G+I ++GDLLRA NPTLA I +FL + ++
Sbjct: 11 AFSLFDRHGTGRIPKTSIGDLLRACGQNPTLAEI---TEIESTLPAEVDMEQFLQVLNRP 67
Query: 512 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 333
G E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K
Sbjct: 68 NGFDMPGDPEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PV 124
Query: 332 DDGMIPYAAFLKKVMA 285
DGM+ Y F++ ++A
Sbjct: 125 KDGMVNYHDFVQMILA 140
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 56.0 bits (129), Expect = 6e-09
Identities = 37/146 (25%), Positives = 70/146 (47%)
Frame = -1
Query: 722 SKNDVERASFAFSXYDFEGKGKIDAFNLGDLLRALNSNPTLATIXXXXXXXXXXXXXXXX 543
SK + AF YD + G I ++G +LR+L N T A +
Sbjct: 4 SKEQTDEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKF 63
Query: 542 XEFLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAE 363
F+ + K ++ + E++++ +++DK+ +G + A+ + LGEKL D+EV
Sbjct: 64 MSFV-----SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQL 118
Query: 362 VTKDCMDPEDDDGMIPYAAFLKKVMA 285
+ ++ DP + G Y F++++MA
Sbjct: 119 MVQEA-DP-TNSGSFDYYDFVQRIMA 142
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 30.7 bits (66), Expect = 0.24
Identities = 34/161 (21%), Positives = 63/161 (39%), Gaps = 1/161 (0%)
Frame = -1
Query: 767 RPGGNRXTPHKMSDLSKNDVERASFAFSXYDFEGKGKIDAFNLGDLLRALNSNPTLATIX 588
R GG ++++ + D+ A F +D + ID L +RAL N + +
Sbjct: 20 RLGGYAPLRVEITEEQRQDINEA---FKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVL 76
Query: 587 XXXXXXXXXXXXXXXXE-FLPIYSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTH 411
E F+ + ++ ++D E+ +L+D +E G + L
Sbjct: 77 KILRDFDKTGKGYLQMEDFVRVMTEKIVERDP--LEEIKRAFELFDDDETGKISLRNLRR 134
Query: 410 TLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAFLKKVM 288
L E +DD E+ + ++ D DG I F+ +M
Sbjct: 135 VAKELNENIDDQELEAMIEEF--DLDQDGEINEQEFIAIMM 173
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.2 bits (55), Expect = 5.2
Identities = 12/30 (40%), Positives = 14/30 (46%)
Frame = +3
Query: 135 PPEXLSPPRALPAPVPQSRASVF*GPSHRT 224
PP PP A P P+P S A P R+
Sbjct: 1721 PPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750
>SPBC13G1.03c |pex14||peroxisomal membrane anchor
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 286
Score = 25.8 bits (54), Expect = 6.9
Identities = 17/48 (35%), Positives = 22/48 (45%)
Frame = +1
Query: 595 VASVGFELSALRRSPRLKASILPLPSKSXIEKAKDALSTSFLLRSLIL 738
V S GF SA + A + PS++ E K+AL FL IL
Sbjct: 77 VISTGFAWSAYSLVKKYIAPMFRAPSQNAYEADKNALDAKFLEAHKIL 124
>SPCC895.08c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 490
Score = 25.8 bits (54), Expect = 6.9
Identities = 19/75 (25%), Positives = 37/75 (49%)
Frame = -2
Query: 715 TTLKGRLSPSQXTTLKAKARSMPSTLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHSKS 536
++L +L+P T KAK+ S+ S S S+ + W + V++ A S S++
Sbjct: 348 SSLSRKLAPPFLRTHKAKSNSLFSMKRPSSSSSSLSGSWHGDTENSVKQSLA---SPSEA 404
Query: 535 SFPSTAKQRKTKTRE 491
S P+ +K + ++
Sbjct: 405 SLPNLSKYSRKNAKK 419
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 6.9
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = -1
Query: 398 LGEKLDDSEVAEVTKDCMDPED 333
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 25.8 bits (54), Expect = 6.9
Identities = 13/29 (44%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
Frame = -1
Query: 734 MSDLSKNDVER-ASFAFSXYDFEGKGKID 651
+++L DV R SF F YDF G G +D
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMD 633
>SPCC584.03c |||RanGTP-binding protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 551
Score = 25.4 bits (53), Expect = 9.1
Identities = 21/75 (28%), Positives = 35/75 (46%), Gaps = 2/75 (2%)
Frame = -2
Query: 715 TTLKGRLSPSQXTTLKAKAR-SMPS-TLAIS*ERSTQTPHWQPSRNSVVQRRRARSCSHS 542
T +PS L+A S+P+ +L S E + + +R ++ ++ S +
Sbjct: 309 TEYSSNTAPSNWIALEALPLVSIPNESLDESDELAESLSDSEAARLQLLGIKKQESVAKK 368
Query: 541 KSSFPSTAKQRKTKT 497
KSSFPST K + T
Sbjct: 369 KSSFPSTIKDQPNLT 383
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,480,695
Number of Sequences: 5004
Number of extensions: 42059
Number of successful extensions: 131
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 371330890
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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