BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P06_pT_I05
(504 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 29 0.52
SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 27 2.1
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 25 6.5
SPAC8C9.09c |mug129||sequence orphan|Schizosaccharomyces pombe|c... 25 6.5
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 25 8.5
SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces po... 25 8.5
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 25 8.5
>SPAC821.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 485
Score = 28.7 bits (61), Expect = 0.52
Identities = 17/49 (34%), Positives = 26/49 (53%), Gaps = 1/49 (2%)
Frame = -2
Query: 287 DDADRELHYQSFKKHLAEINQLNEKNPYTTFGINKF-ADYTPEEQQSRL 144
DD+D E Y+ + +L++KN Y+TF ++F TP QS L
Sbjct: 367 DDSDSERSYRRVRDQYLSKPRLSDKNRYSTF--SEFPGQGTPSASQSNL 413
>SPBC211.03c |||guanyl-nucleotide exchange
factor|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1462
Score = 26.6 bits (56), Expect = 2.1
Identities = 17/33 (51%), Positives = 20/33 (60%)
Frame = +3
Query: 129 RQPQSQPALLFLRSIISKFVNAKRSIWILLVQL 227
R ++Q ALL L I SKF N + W LLVQL
Sbjct: 866 RDFRAQLALLVLFWISSKFGNIIDASWPLLVQL 898
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 25.0 bits (52), Expect = 6.5
Identities = 15/52 (28%), Positives = 25/52 (48%)
Frame = +3
Query: 36 ILNILQAVTIHLFRLEXHSDRLKSQNLCLLSRQPQSQPALLFLRSIISKFVN 191
++NI + L R SD L + LL R+P + A +R I+++ N
Sbjct: 99 MINIFEVFLPQLLRYPNASDPLNGEAAALLLREPNTYYAK--VRDYIARYAN 148
>SPAC8C9.09c |mug129||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 302
Score = 25.0 bits (52), Expect = 6.5
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = -1
Query: 279 RQRAALSIIQETLGRDKPIEREESIYYVW 193
R+RAALS + P+ +ES +Y W
Sbjct: 154 RKRAALSKLGHPAFHFLPMNNKESFHYAW 182
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 24.6 bits (51), Expect = 8.5
Identities = 8/22 (36%), Positives = 11/22 (50%)
Frame = -2
Query: 269 LHYQSFKKHLAEINQLNEKNPY 204
+H FK H+A + NPY
Sbjct: 1295 MHIDCFKNHIATVTLATRANPY 1316
>SPAC222.10c |byr4||two-component GAP Byr4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 665
Score = 24.6 bits (51), Expect = 8.5
Identities = 13/44 (29%), Positives = 26/44 (59%), Gaps = 4/44 (9%)
Frame = -2
Query: 305 HNRE-YKDDADRELHYQS---FKKHLAEINQLNEKNPYTTFGIN 186
HN + + DD D + + ++ F+K + N +N+K+PY + I+
Sbjct: 221 HNDDGFWDDFDIDFNNETESIFRKKIRSPNTINQKHPYISSTIS 264
>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 321
Score = 24.6 bits (51), Expect = 8.5
Identities = 13/32 (40%), Positives = 19/32 (59%)
Frame = -3
Query: 364 LRDIMTSIKPKNCSKFSSKNITGNTRMMPTES 269
L+D+ +S+K K SK SS + T + TES
Sbjct: 76 LKDMFSSMKKKKKSKKSSASAEEQTEDITTES 107
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,957,572
Number of Sequences: 5004
Number of extensions: 37081
Number of successful extensions: 117
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 116
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 117
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 200198394
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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