Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P06_pT_D07
(737 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 217 2e-57
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 53 5e-08
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 51 2e-07
SPAC1F3.03 |||Lgl family protein|Schizosaccharomyces pombe|chr 1... 29 0.69
SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces ... 28 1.2
SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit ... 28 1.6
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 27 2.1
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 27 2.1
SPCP31B10.03c |med31|soh1, sep10|mediator complex subunit Med31|... 27 2.8
SPCC18B5.07c |nup61||nucleoporin Nup61|Schizosaccharomyces pombe... 27 3.7
SPBC12C2.02c |ste20|ste16|sterility protein Ste20|Schizosaccharo... 26 4.9
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 26 4.9
SPAC14C4.16 |dad3||DASH complex subunit Dad3|Schizosaccharomyces... 25 8.5
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 217 bits (529), Expect = 2e-57
Identities = 101/163 (61%), Positives = 133/163 (81%)
Frame = -1
Query: 713 APKIRKTTVLDVMQRLLQPKNMMVSLSPDRANQHCYISILNIIQGEVEPSQVHKSLQRIR 534
A IRKTTVLDVM+RLL PKN MVS++P + + C+ISIL+IIQGE +P+ VHKSL RIR
Sbjct: 284 AKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKS--CFISILDIIQGEADPADVHKSLLRIR 341
Query: 533 ERRLAAFIPWGPASVQVALSRRSPHVTAAHKVSGLLLANHTNISSLFDRCLQQFDKLRKR 354
ERR A+FIPWGPAS+QVALS++SP++ H+VSGL+LANHT+I+SLF R L Q+D+LRKR
Sbjct: 342 ERRYASFIPWGPASIQVALSKKSPYIKTNHRVSGLMLANHTSIASLFKRTLDQYDRLRKR 401
Query: 353 EAFLEVFRKEPMFRESLDELDASRGVVDELVHEYRAAATPDYV 225
AFLE ++KE +F + L+E D+SR VV +L++EY A P+Y+
Sbjct: 402 NAFLEQYKKEAIFEDDLNEFDSSRDVVADLINEYEACEDPNYL 444
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 52.8 bits (121), Expect = 5e-08
Identities = 38/144 (26%), Positives = 73/144 (50%), Gaps = 9/144 (6%)
Frame = -1
Query: 692 TVLDVMQRLLQPKNMMVSLSPDRANQHCYISILNIIQGEVEPSQVHKSLQRIRERRLAAF 513
+V ++ + +P N MV P RA + Y++ + +G+V P V ++ I+ +R F
Sbjct: 287 SVQEITNQCFEPYNQMVKCDP-RAGR--YMATCLLYRGDVIPRDVQAAVTTIKAKRTIQF 343
Query: 512 IPWGPASVQVALSRRSP-HVTAAH--KV--SGLLLANHTNISSLFDRCLQQFDKLRKREA 348
+ W P ++ + R P H+ + KV + +L+N T+I+ + R +FD + + A
Sbjct: 344 VDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRA 403
Query: 347 FLEVFRKEPM----FRESLDELDA 288
F+ + E M F E+ ++L A
Sbjct: 404 FVHWYVGEGMEEGEFSEAREDLAA 427
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 50.8 bits (116), Expect = 2e-07
Identities = 37/144 (25%), Positives = 69/144 (47%), Gaps = 9/144 (6%)
Frame = -1
Query: 692 TVLDVMQRLLQPKNMMVSLSPDRANQHCYISILNIIQGEVEPSQVHKSLQRIRERRLAAF 513
+V ++ + +P N MV P Y++ + +G+V P V ++ I+ RR F
Sbjct: 291 SVQEITNQCFEPYNQMVKCDPRTGR---YMATCLLYRGDVIPRDVQAAVTSIKSRRTIQF 347
Query: 512 IPWGPASVQVALSRRSP-HV--TAAHKVSG--LLLANHTNISSLFDRCLQQFDKLRKREA 348
+ W P ++ + P HV + KV+ +L+N T+I+ + R +FD + + A
Sbjct: 348 VDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRA 407
Query: 347 FLEVFRKEPM----FRESLDELDA 288
F+ + E M F E+ ++L A
Sbjct: 408 FVHWYVGEGMEEGEFSEAREDLAA 431
>SPAC1F3.03 |||Lgl family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1004
Score = 29.1 bits (62), Expect = 0.69
Identities = 15/47 (31%), Positives = 20/47 (42%)
Frame = -1
Query: 530 RRLAAFIPWGPASVQVALSRRSPHVTAAHKVSGLLLANHTNISSLFD 390
+R FI P + + SPH +H LLL + N SL D
Sbjct: 367 QRFFPFIDIPPVRDMLVIPSSSPHYNGSHNPKNLLLLSEDNSLSLLD 413
>SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 2493
Score = 28.3 bits (60), Expect = 1.2
Identities = 13/69 (18%), Positives = 32/69 (46%)
Frame = -1
Query: 458 VTAAHKVSGLLLANHTNISSLFDRCLQQFDKLRKREAFLEVFRKEPMFRESLDELDASRG 279
+ AHK+ ++ S F CL++++ L ++ R + +SL ++ +
Sbjct: 26 IDVAHKIPRAADLEDEDVESYFISCLRKWEDLNLSTHYVNFLRSVTPYSQSLPQIVYHQK 85
Query: 278 VVDELVHEY 252
+ +L+ +Y
Sbjct: 86 TIFDLIVQY 94
>SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit
Pmc1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 879
Score = 27.9 bits (59), Expect = 1.6
Identities = 18/44 (40%), Positives = 24/44 (54%)
Frame = +3
Query: 66 LRYKLNCQRLRIIDLTTHLWHSSLNSAGDELRDMNPTFRRLYVL 197
+RY+L+ RLR DL T L + S G LR N +LY+L
Sbjct: 104 IRYQLSFARLRNSDLVTAL---DILSTGTSLRLANAPTSKLYML 144
>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1428
Score = 27.5 bits (58), Expect = 2.1
Identities = 17/57 (29%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = -1
Query: 713 APKIRKTTVLDVMQRLLQPKNMMVSLSPDRANQHCYISILNIIQGEVEPSQV-HKSL 546
APKI +TT + + +R + N++ + + ISI + G + P+Q H SL
Sbjct: 944 APKIFETTGIPISKRAKKALNLITPGELNNKGKVALISINKLEDGSIIPTQFSHSSL 1000
>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1067
Score = 27.5 bits (58), Expect = 2.1
Identities = 17/71 (23%), Positives = 35/71 (49%), Gaps = 2/71 (2%)
Frame = -1
Query: 362 RKREAFLEVFRKEPMFRESLDELDASRGVVDELVHEYRAAATPDYVH--WNPESSQI*HI 189
++ + +L + + + RE ELD+ +V E + + PD+V +N E S+ H+
Sbjct: 444 QRHKEYLLTEQLKQIKRELGQELDSKEALVTEFKKRTESLSMPDHVKKVFNDELSKFQHL 503
Query: 188 QATECWIHITK 156
+ +IT+
Sbjct: 504 EPMAAEFNITR 514
>SPCP31B10.03c |med31|soh1, sep10|mediator complex subunit
Med31|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 27.1 bits (57), Expect = 2.8
Identities = 11/34 (32%), Positives = 16/34 (47%)
Frame = -1
Query: 329 KEPMFRESLDELDASRGVVDELVHEYRAAATPDY 228
K P FR + D S+ V DE+ +E+ Y
Sbjct: 79 KNPQFRNDISRADLSKQVNDEIYYEWLGKGLQQY 112
>SPCC18B5.07c |nup61||nucleoporin Nup61|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 565
Score = 26.6 bits (56), Expect = 3.7
Identities = 20/72 (27%), Positives = 32/72 (44%), Gaps = 2/72 (2%)
Frame = -1
Query: 410 NISSLFDRCLQQFDKLRKREAFLEVFRKEPMFRESLD-ELDASRGVVDEL-VHEYRAAAT 237
N+S LFD Q F + K+ A F F ++ E S VV E+ + ++ +
Sbjct: 118 NLSPLFDEYRQHFSSIEKKPAEGNAFIVSTSFLSNVFLEQPTSNAVVSEVNPQQQKSQDS 177
Query: 236 PDYVHWNPESSQ 201
+V P SS+
Sbjct: 178 SSFVTEKPASSE 189
>SPBC12C2.02c |ste20|ste16|sterility protein
Ste20|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1309
Score = 26.2 bits (55), Expect = 4.9
Identities = 16/41 (39%), Positives = 25/41 (60%)
Frame = -1
Query: 383 LQQFDKLRKREAFLEVFRKEPMFRESLDELDASRGVVDELV 261
LQ FD K+E RKE + ++ DEL++S+ ++ELV
Sbjct: 61 LQVFDTNFKKET---KERKEMLKKKCTDELESSKKKIEELV 98
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 26.2 bits (55), Expect = 4.9
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -2
Query: 259 TSTAPPPRPTTCTGTQRAAKYNT 191
TST PPP T+ TGT + +T
Sbjct: 367 TSTPPPPASTSSTGTSSSPLLST 389
Score = 25.4 bits (53), Expect = 8.5
Identities = 10/15 (66%), Positives = 11/15 (73%)
Frame = -2
Query: 259 TSTAPPPRPTTCTGT 215
TST PPP T+ TGT
Sbjct: 313 TSTPPPPASTSSTGT 327
>SPAC14C4.16 |dad3||DASH complex subunit Dad3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 86
Score = 25.4 bits (53), Expect = 8.5
Identities = 10/39 (25%), Positives = 21/39 (53%)
Frame = +3
Query: 99 IIDLTTHLWHSSLNSAGDELRDMNPTFRRLYVLYLAALW 215
++ + + ++ N+ D LRD+ +Y LY A++W
Sbjct: 27 LVKVLQDMVYNPSNNILDSLRDLEKEVGLVYTLYKASVW 65
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,559,785
Number of Sequences: 5004
Number of extensions: 45588
Number of successful extensions: 178
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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