Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= fcaL-P06_F_N20
(667 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 57 3e-09
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 46 5e-06
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 43 3e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 29 0.46
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.4
SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 5.6
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 56.8 bits (131), Expect = 3e-09
Identities = 29/75 (38%), Positives = 44/75 (58%)
Frame = +3
Query: 279 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 458
A+K KD E+ E K++DK+ NG + ELTH L +LGE+L EVA++ ++
Sbjct: 75 ARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA--DT 132
Query: 459 DDDGMIPYAAFLKKV 503
D DG+I Y F + +
Sbjct: 133 DGDGVINYEEFSRVI 147
Score = 29.9 bits (64), Expect = 0.34
Identities = 18/68 (26%), Positives = 36/68 (52%)
Frame = +3
Query: 312 DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAAF 491
+F E L+D++++G + EL + +LG+ +E+ ++ + D +G I + F
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEV--DADGNGTIDFTEF 70
Query: 492 LKKVMAXK 515
L +MA K
Sbjct: 71 L-TMMARK 77
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 46.0 bits (104), Expect = 5e-06
Identities = 33/128 (25%), Positives = 58/128 (45%)
Frame = +3
Query: 126 GKGKLXAFNXXXXXXXXNSNPTLATIXXXXXXXXXXXXXXXXXXFLPIYSQAKKDKDQGA 305
G G++ + NPTLA I FL + ++ G
Sbjct: 19 GTGRIPKTSIGDLLRACGQNPTLAEITEIESTLPAEVDMEQ---FLQVLNRPNGFDMPGD 75
Query: 306 YEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYA 485
E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K DGM+ Y
Sbjct: 76 PEEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PVKDGMVNYH 132
Query: 486 AFLKKVMA 509
F++ ++A
Sbjct: 133 DFVQMILA 140
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 43.2 bits (97), Expect = 3e-05
Identities = 21/77 (27%), Positives = 46/77 (59%)
Frame = +3
Query: 279 AKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPE 458
+ K ++ + E++++ +++DK+ +G + A+ + LGEKL D+EV + ++ DP
Sbjct: 68 SNKLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP- 125
Query: 459 DDDGMIPYAAFLKKVMA 509
+ G Y F++++MA
Sbjct: 126 TNSGSFDYYDFVQRIMA 142
Score = 30.7 bits (66), Expect = 0.20
Identities = 14/60 (23%), Positives = 34/60 (56%)
Frame = +3
Query: 309 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEDDDGMIPYAA 488
++ E LYD +++GL+ + + L +LG + D+E+A+++ + D D+ + + +
Sbjct: 9 DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGDAIDEKKFMSFVS 68
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 29.5 bits (63), Expect = 0.46
Identities = 15/58 (25%), Positives = 30/58 (51%)
Frame = +3
Query: 270 YSQAKKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKD 443
Y+ + + + +D E KL+D +++ + EL + ALG + SEV ++ +D
Sbjct: 24 YAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALGFNAEKSEVLKILRD 81
Score = 27.1 bits (57), Expect = 2.4
Identities = 18/75 (24%), Positives = 33/75 (44%)
Frame = +3
Query: 282 KKDKDQGAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPED 461
+K ++ E+ +L+D +E G + L L E +DD E+ + ++ D
Sbjct: 101 EKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEF--DLD 158
Query: 462 DDGMIPYAAFLKKVM 506
DG I F+ +M
Sbjct: 159 QDGEINEQEFIAIMM 173
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 1.4
Identities = 13/31 (41%), Positives = 15/31 (48%)
Frame = -3
Query: 662 APPEELSPPRALPAPVPQSRASVF*GPSHRT 570
APP PP A P P+P S A P R+
Sbjct: 1720 APPMPAGPPSAPPPPLPASSAPSVPNPGDRS 1750
>SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1142
Score = 25.8 bits (54), Expect = 5.6
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = +3
Query: 396 LGEKLDDSEVAEVTKDCMDPED 461
L EK+ D + + DC+DP+D
Sbjct: 777 LAEKVKDFQTMVILLDCLDPKD 798
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,136,656
Number of Sequences: 5004
Number of extensions: 35799
Number of successful extensions: 107
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 96
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 107
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 303841898
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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